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The map label for this gene is ruvB

Identifier: 88608213

GI number: 88608213

Start: 501204

End: 502199

Strand: Direct

Name: ruvB

Synonym: NSE_0575

Alternate gene names: 88608213

Gene position: 501204-502199 (Clockwise)

Preceding gene: 88608038

Following gene: 88608381

Centisome position: 58.35

GC content: 41.57

Gene sequence:

>996_bases
GTGCGAAATAGTATATTTGAACAAGAGGAGACTTTCCAGGACCTTTCACTTAGACCGAAATCTATCGAAAAGTTCGTTGG
ACAGCAACGTGTCGTAGAAAACTTGCAAGTTTTTATAGACTCCGCACAAAAAAGGAATGACTCCCTTGATCACGTACTTT
TCTGTGGTCCACCAGGTCTCGGAAAAACAACATTAGCACATATTATAAGTAACGAGCTTGAAAGTAGAATCCACACAACC
GCTGGACCGCTTCTCTCAAAGGCTGGCGATATTGCAGCAATACTTACAAACTTGCACAAAAATGACATCCTCTTCATAGA
TGAAATACATAGACTACCTTCAGCTGTAGAAGAAGTCCTCTATCCCGCTATGGAGGACTACCACCTTGATCTCATTGTCG
GAGATGGACCAGCAGCAAAGAGTATTAGAATAAATCTAGCTAAATTCACACTAGTTGCCGCAACTACGCGTATAGGGATG
CTCTCCAATCCACTCAGAGATAGATTTGGTATTACACTTAGGCTCGACTTTTATACTGTAAGCGAACTACTTCAACTTCT
TCAGCAAGCAGCAGAAAGGTTATCTGTTAATATAGAAAATGGCGCCATGATAGAGCTTGCAAAACGCTCTAGAGGAACAC
CAAGAATCGCTCTTAGGCTGCTCAAAAGGGTGCGTGACTTTCTTGAAGTCAGCGATTCTGATGTCATAACTCGCGAATTT
GCAGACCTAGCACTGAATAAGATGGAGGTAGACCAATTCGGTTTAGATAAGTTAGACTACACGTATATGGATTTCATAGC
AAAAAATTATTCTGATAATCCGGTGGGTATCAAAACCATTGCTGCAGCAATCTCGGAAAAAGAGGATTCGATAGAAGAGT
TAATAGAGCCATACTTGATAAAAATAGGCTTTCTGAGTCGTACACAACGGGGTCGTCGACTAACGGGAAAAGCATTAGAC
TACTTGTCAACAATTAATTCAGCAAGGCTGCCCTGA

Upstream 100 bases:

>100_bases
CACATCAGGAATTCAAGGGAAATAAGACCATCATCACCACTATTTTGGTGATGGAAAGTATAGTAAAGTTACGTTAGCTG
CTGGCCCCAACCAAGAGAAA

Downstream 100 bases:

>100_bases
GAAAAAGATTCGTATGGGATATGAAGTTATATTTGCTTTTGTTCTGCAGGTAGGAATAGCATATTCAGTGTTCTTCTTAG
GAGAAGTCCAAATAAGGGAC

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MRNSIFEQEETFQDLSLRPKSIEKFVGQQRVVENLQVFIDSAQKRNDSLDHVLFCGPPGLGKTTLAHIISNELESRIHTT
AGPLLSKAGDIAAILTNLHKNDILFIDEIHRLPSAVEEVLYPAMEDYHLDLIVGDGPAAKSIRINLAKFTLVAATTRIGM
LSNPLRDRFGITLRLDFYTVSELLQLLQQAAERLSVNIENGAMIELAKRSRGTPRIALRLLKRVRDFLEVSDSDVITREF
ADLALNKMEVDQFGLDKLDYTYMDFIAKNYSDNPVGIKTIAAAISEKEDSIEELIEPYLIKIGFLSRTQRGRRLTGKALD
YLSTINSARLP

Sequences:

>Translated_331_residues
MRNSIFEQEETFQDLSLRPKSIEKFVGQQRVVENLQVFIDSAQKRNDSLDHVLFCGPPGLGKTTLAHIISNELESRIHTT
AGPLLSKAGDIAAILTNLHKNDILFIDEIHRLPSAVEEVLYPAMEDYHLDLIVGDGPAAKSIRINLAKFTLVAATTRIGM
LSNPLRDRFGITLRLDFYTVSELLQLLQQAAERLSVNIENGAMIELAKRSRGTPRIALRLLKRVRDFLEVSDSDVITREF
ADLALNKMEVDQFGLDKLDYTYMDFIAKNYSDNPVGIKTIAAAISEKEDSIEELIEPYLIKIGFLSRTQRGRRLTGKALD
YLSTINSARLP
>Mature_331_residues
MRNSIFEQEETFQDLSLRPKSIEKFVGQQRVVENLQVFIDSAQKRNDSLDHVLFCGPPGLGKTTLAHIISNELESRIHTT
AGPLLSKAGDIAAILTNLHKNDILFIDEIHRLPSAVEEVLYPAMEDYHLDLIVGDGPAAKSIRINLAKFTLVAATTRIGM
LSNPLRDRFGITLRLDFYTVSELLQLLQQAAERLSVNIENGAMIELAKRSRGTPRIALRLLKRVRDFLEVSDSDVITREF
ADLALNKMEVDQFGLDKLDYTYMDFIAKNYSDNPVGIKTIAAAISEKEDSIEELIEPYLIKIGFLSRTQRGRRLTGKALD
YLSTINSARLP

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family

Homologues:

Organism=Escherichia coli, GI1788167, Length=322, Percent_Identity=50, Blast_Score=335, Evalue=3e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVB_NEOSM (Q2GDJ0)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_506456.1
- ProteinModelPortal:   Q2GDJ0
- SMR:   Q2GDJ0
- STRING:   Q2GDJ0
- GeneID:   3931683
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0575
- TIGR:   NSE_0575
- eggNOG:   COG2255
- HOGENOM:   HBG339960
- OMA:   EKFDGGP
- ProtClustDB:   PRK00080
- BioCyc:   NSEN222891:NSE_0575-MONOMER
- HAMAP:   MF_00016
- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- ProDom:   PD005323
- SMART:   SM00382
- TIGRFAMs:   TIGR00635

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N

EC number: =3.6.4.12

Molecular weight: Translated: 37214; Mature: 37214

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNSIFEQEETFQDLSLRPKSIEKFVGQQRVVENLQVFIDSAQKRNDSLDHVLFCGPPGL
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCC
GKTTLAHIISNELESRIHTTAGPLLSKAGDIAAILTNLHKNDILFIDEIHRLPSAVEEVL
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHH
YPAMEDYHLDLIVGDGPAAKSIRINLAKFTLVAATTRIGMLSNPLRDRFGITLRLDFYTV
HHHHCCCEEEEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCHHHHCCEEEEEHHHHH
SELLQLLQQAAERLSVNIENGAMIELAKRSRGTPRIALRLLKRVRDFLEVSDSDVITREF
HHHHHHHHHHHHHHEEECCCCCEEEEHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
ADLALNKMEVDQFGLDKLDYTYMDFIAKNYSDNPVGIKTIAAAISEKEDSIEELIEPYLI
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHH
KIGFLSRTQRGRRLTGKALDYLSTINSARLP
HHHHHHHCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRNSIFEQEETFQDLSLRPKSIEKFVGQQRVVENLQVFIDSAQKRNDSLDHVLFCGPPGL
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCC
GKTTLAHIISNELESRIHTTAGPLLSKAGDIAAILTNLHKNDILFIDEIHRLPSAVEEVL
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHH
YPAMEDYHLDLIVGDGPAAKSIRINLAKFTLVAATTRIGMLSNPLRDRFGITLRLDFYTV
HHHHCCCEEEEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCHHHHCCEEEEEHHHHH
SELLQLLQQAAERLSVNIENGAMIELAKRSRGTPRIALRLLKRVRDFLEVSDSDVITREF
HHHHHHHHHHHHHHEEECCCCCEEEEHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
ADLALNKMEVDQFGLDKLDYTYMDFIAKNYSDNPVGIKTIAAAISEKEDSIEELIEPYLI
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHH
KIGFLSRTQRGRRLTGKALDYLSTINSARLP
HHHHHHHCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA