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The map label for this gene is fumC

Identifier: 161511033

GI number: 161511033

Start: 48009

End: 49421

Strand: Reverse

Name: fumC

Synonym: NSE_0056

Alternate gene names: 161511033

Gene position: 49421-48009 (Counterclockwise)

Preceding gene: 88608486

Following gene: 88608626

Centisome position: 5.75

GC content: 42.53

Gene sequence:

>1413_bases
ATGGATTATAGAATTGAAACTGATTCCATCGGATCGATAAAAGTTCCGCAGGATAAGTATTGGGGCGCACAAACTCAGAG
AGCAATGGAAAATTTCAAAATAGGGACAGAAAAGGTTCCTATGGAAGTAGTAAAGGCCATCGCAATAATTAAACTCGCTG
CGGCGCGCACAAATGTAAAAGCAGGTGCATTATCAAAGGATTACCTTGGAGCAATTGAAGAAGCGTGTCACGAGATTATC
GATGGGAAACTAGATGATCAGTTCCAGCTTTCGGTCTGGCAGACAGGGTCCGGAACTCAGACGAACATGAATGTTAATGA
AGTTATCTCTAATCGGGCTATAGAGAAGTTTAATGGTGTGATCGGCAGCAAAACCCCAATCCATCCAAACGATCATGTTA
ACATGGCGCAATCATCAAATGACACCTTTCCCTCTGCAATGCATATTGCAGCTGTTGAAAAGGTACTCAAAAAACTTCTC
CCCTCACTTTTTTACCTTGTTGAGGTGTTATCAGAAAAAATAAAAAGTTTTTCTGGCATTGTTAAGATCGGGAGGACTCA
TATGCAGGATGCAGTTCCTATCACATTAAGTCAGGAGTTCTCATGTTTTTGTGAACAACTAAATAAAAGCGCATCCCGTT
TGAATGAAAATTTATCTAGGCTCCTTGAAATACCACAAGGAGGTACGGCTGTCGGTACTGGGTTAAACACGAGGAGGAAC
TTCGATCATGAGATCGTCGCTGAAATCGCAAGAATTACTTCTATAGAGTTCACACCTGCGAAAAACAAGTGTGAGATGCT
TGCCTCACACGACACATTAGTTGAGTTAAGCGGTAATTTAAATACGTTGGCTGTGTCGTTGATGAAAATAGCGAATGACA
TTCGATTACTCGGTTCTGGTCCGAGGTGTGGTATAGGAGAACTTATATTACCGATGAATGAACCCGGCTCATCAATTATG
CCAGGAAAGGTGAATCCAACCCAGTGCGAAGCCTTAACAATGGTTTGTGCGCAGGTCATCGGAAATCACTCAGCCGTAAC
TGTAGCTGGTGCTGGCGGCCAATTACAGCTCAACACATTCAAACCAATGATCATATACAATCTGCTACAATCAATGGATT
TACTTTCAGATGGAATGATCAGTTTCGGAGAGAAATGTGTAATGGGAATAGTGCCGAATAAAAAGCGAATAGACAACATG
TTACAGGAATCGTTGATGCTGATTACTGCTTTGAACAAGGAAATTGGATACGACAACGCAGCAAAGATTGCGAAATTTGC
CTACTCAAAGGATTGCACGCTTAAGGAAGCAGCCATGGAACTCGAACTTCTCTCCGAAGATGAGTTTGACAGGATTGTCA
ACCCCGCACACATGCTAGGACCAGACCAACAAGAGAAAACGGGCCCAGCTTAA

Upstream 100 bases:

>100_bases
TTGCTTTTGTAAATCTTTCTTTAAGCAAACTCTAGCTGAACCCTGCCGGAAGGATTATAATCGGCCTATGCTGGTGCACT
TTGGTTTTGCTTACAGAGAC

Downstream 100 bases:

>100_bases
GGCACTAGGGTACGGCTGCTGCATTTCTGCCCTGACCGGGTTCGCCTGGCACCTTACCTGGACCTAAAAAAGGATAGCAT
CCCCTTAGTGTTTGTGCAAT

Product: fumarate hydratase

Products: NA

Alternate protein names: Fumarase C 1 [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MDYRIETDSIGSIKVPQDKYWGAQTQRAMENFKIGTEKVPMEVVKAIAIIKLAAARTNVKAGALSKDYLGAIEEACHEII
DGKLDDQFQLSVWQTGSGTQTNMNVNEVISNRAIEKFNGVIGSKTPIHPNDHVNMAQSSNDTFPSAMHIAAVEKVLKKLL
PSLFYLVEVLSEKIKSFSGIVKIGRTHMQDAVPITLSQEFSCFCEQLNKSASRLNENLSRLLEIPQGGTAVGTGLNTRRN
FDHEIVAEIARITSIEFTPAKNKCEMLASHDTLVELSGNLNTLAVSLMKIANDIRLLGSGPRCGIGELILPMNEPGSSIM
PGKVNPTQCEALTMVCAQVIGNHSAVTVAGAGGQLQLNTFKPMIIYNLLQSMDLLSDGMISFGEKCVMGIVPNKKRIDNM
LQESLMLITALNKEIGYDNAAKIAKFAYSKDCTLKEAAMELELLSEDEFDRIVNPAHMLGPDQQEKTGPA

Sequences:

>Translated_470_residues
MDYRIETDSIGSIKVPQDKYWGAQTQRAMENFKIGTEKVPMEVVKAIAIIKLAAARTNVKAGALSKDYLGAIEEACHEII
DGKLDDQFQLSVWQTGSGTQTNMNVNEVISNRAIEKFNGVIGSKTPIHPNDHVNMAQSSNDTFPSAMHIAAVEKVLKKLL
PSLFYLVEVLSEKIKSFSGIVKIGRTHMQDAVPITLSQEFSCFCEQLNKSASRLNENLSRLLEIPQGGTAVGTGLNTRRN
FDHEIVAEIARITSIEFTPAKNKCEMLASHDTLVELSGNLNTLAVSLMKIANDIRLLGSGPRCGIGELILPMNEPGSSIM
PGKVNPTQCEALTMVCAQVIGNHSAVTVAGAGGQLQLNTFKPMIIYNLLQSMDLLSDGMISFGEKCVMGIVPNKKRIDNM
LQESLMLITALNKEIGYDNAAKIAKFAYSKDCTLKEAAMELELLSEDEFDRIVNPAHMLGPDQQEKTGPA
>Mature_470_residues
MDYRIETDSIGSIKVPQDKYWGAQTQRAMENFKIGTEKVPMEVVKAIAIIKLAAARTNVKAGALSKDYLGAIEEACHEII
DGKLDDQFQLSVWQTGSGTQTNMNVNEVISNRAIEKFNGVIGSKTPIHPNDHVNMAQSSNDTFPSAMHIAAVEKVLKKLL
PSLFYLVEVLSEKIKSFSGIVKIGRTHMQDAVPITLSQEFSCFCEQLNKSASRLNENLSRLLEIPQGGTAVGTGLNTRRN
FDHEIVAEIARITSIEFTPAKNKCEMLASHDTLVELSGNLNTLAVSLMKIANDIRLLGSGPRCGIGELILPMNEPGSSIM
PGKVNPTQCEALTMVCAQVIGNHSAVTVAGAGGQLQLNTFKPMIIYNLLQSMDLLSDGMISFGEKCVMGIVPNKKRIDNM
LQESLMLITALNKEIGYDNAAKIAKFAYSKDCTLKEAAMELELLSEDEFDRIVNPAHMLGPDQQEKTGPA

Specific function: Tricarboxylic acid cycle [C]

COG id: COG0114

COG function: function code C; Fumarase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Fumarase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=461, Percent_Identity=60.0867678958785, Blast_Score=550, Evalue=1e-157,
Organism=Homo sapiens, GI266458395, Length=77, Percent_Identity=49.3506493506493, Blast_Score=73, Evalue=7e-13,
Organism=Escherichia coli, GI1787896, Length=457, Percent_Identity=54.9234135667396, Blast_Score=507, Evalue=1e-145,
Organism=Escherichia coli, GI87082375, Length=466, Percent_Identity=39.0557939914163, Blast_Score=313, Evalue=2e-86,
Organism=Escherichia coli, GI1790398, Length=334, Percent_Identity=23.9520958083832, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17553882, Length=460, Percent_Identity=58.2608695652174, Blast_Score=551, Evalue=1e-157,
Organism=Caenorhabditis elegans, GI32565146, Length=327, Percent_Identity=58.4097859327217, Blast_Score=396, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6324993, Length=461, Percent_Identity=57.2668112798265, Blast_Score=525, Evalue=1e-150,
Organism=Drosophila melanogaster, GI24640179, Length=463, Percent_Identity=57.0194384449244, Blast_Score=528, Evalue=1e-150,
Organism=Drosophila melanogaster, GI24640177, Length=463, Percent_Identity=57.0194384449244, Blast_Score=527, Evalue=1e-150,
Organism=Drosophila melanogaster, GI78710009, Length=463, Percent_Identity=54.4276457883369, Blast_Score=511, Evalue=1e-145,
Organism=Drosophila melanogaster, GI24662684, Length=465, Percent_Identity=54.6236559139785, Blast_Score=509, Evalue=1e-144,
Organism=Drosophila melanogaster, GI24583245, Length=465, Percent_Identity=50.1075268817204, Blast_Score=470, Evalue=1e-133,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005677
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 51287; Mature: 51287

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYRIETDSIGSIKVPQDKYWGAQTQRAMENFKIGTEKVPMEVVKAIAIIKLAAARTNVK
CCCEECCCCCCCEECCCHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCC
AGALSKDYLGAIEEACHEIIDGKLDDQFQLSVWQTGSGTQTNMNVNEVISNRAIEKFNGV
CCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCC
IGSKTPIHPNDHVNMAQSSNDTFPSAMHIAAVEKVLKKLLPSLFYLVEVLSEKIKSFSGI
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKIGRTHMQDAVPITLSQEFSCFCEQLNKSASRLNENLSRLLEIPQGGTAVGTGLNTRRN
HHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCC
FDHEIVAEIARITSIEFTPAKNKCEMLASHDTLVELSGNLNTLAVSLMKIANDIRLLGSG
CCHHHHHHHHHHHCCEECCCCCHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHEECCC
PRCGIGELILPMNEPGSSIMPGKVNPTQCEALTMVCAQVIGNHSAVTVAGAGGQLQLNTF
CCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEECCC
KPMIIYNLLQSMDLLSDGMISFGEKCVMGIVPNKKRIDNMLQESLMLITALNKEIGYDNA
CHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHH
AKIAKFAYSKDCTLKEAAMELELLSEDEFDRIVNPAHMLGPDQQEKTGPA
HHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHCCCHHHCCCCCCCCCCCC
>Mature Secondary Structure
MDYRIETDSIGSIKVPQDKYWGAQTQRAMENFKIGTEKVPMEVVKAIAIIKLAAARTNVK
CCCEECCCCCCCEECCCHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCC
AGALSKDYLGAIEEACHEIIDGKLDDQFQLSVWQTGSGTQTNMNVNEVISNRAIEKFNGV
CCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCC
IGSKTPIHPNDHVNMAQSSNDTFPSAMHIAAVEKVLKKLLPSLFYLVEVLSEKIKSFSGI
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKIGRTHMQDAVPITLSQEFSCFCEQLNKSASRLNENLSRLLEIPQGGTAVGTGLNTRRN
HHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCC
FDHEIVAEIARITSIEFTPAKNKCEMLASHDTLVELSGNLNTLAVSLMKIANDIRLLGSG
CCHHHHHHHHHHHCCEECCCCCHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHEECCC
PRCGIGELILPMNEPGSSIMPGKVNPTQCEALTMVCAQVIGNHSAVTVAGAGGQLQLNTF
CCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEECCC
KPMIIYNLLQSMDLLSDGMISFGEKCVMGIVPNKKRIDNMLQESLMLITALNKEIGYDNA
CHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHH
AKIAKFAYSKDCTLKEAAMELELLSEDEFDRIVNPAHMLGPDQQEKTGPA
HHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHCCCHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1856685; 12597275 [H]