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The map label for this gene is pnp

Identifier: 88608486

GI number: 88608486

Start: 49726

End: 51999

Strand: Reverse

Name: pnp

Synonym: NSE_0057

Alternate gene names: 88608486

Gene position: 51999-49726 (Counterclockwise)

Preceding gene: 88608422

Following gene: 161511033

Centisome position: 6.05

GC content: 45.82

Gene sequence:

>2274_bases
ATGTTTGAAATAAAAGAATCCTCCATGGAATGGGAGGGGAAGAGTTTGCACATAAAAACCGGAGAGGTAGCACGTCAGGC
TGCTGGTGCAGCTTGCGTTTCATACGGTGGAACCGTTGTGCTGGCTGTAGTAACTCTTCAGAAGGATACTGCAGCTTCTA
AAAAGACTTCTGACTTGTCGGGTCTAGCACTTGTGACAAACTTCCTAGCCAAATCATACGCTCTGGGGCGTATACCAAAC
GGCTTCTTCAAACGGGAAGGCAAGCTCTCAGAGAGAGAGGTGTTGGCCTCCAGGGTGGTTGATAGAGCCGTAAGACCCTT
GATAGAAGAAAATCTCGTTAATGAGGTAAACATAGTGTGCAAACTTCTTGCACATGGCAATAGAAAGGTTCTACCTGAAA
TACCAGCTCTGATAGCGGCCTCCGCTGCGTTGCAGCTCTCTGGAATTCCATTTAGCGGTCCCGTTATCGGGGTCGATGTT
GACATGCGTGGGTCAGAAGTCACATGTAACGAGATCAGGGAGACAGAAGGTGGACTCGAACTCTTTGTTGCTTGTACAGA
GGAATCGGTTGTAATGGTGGAAGCCGAAGCAAGTGAAGCCTCTGAAGAGGAGGTTGTAAATGCCCTTTCAGAGGCGCTAA
AAAGCGCAAAACCGGTTTTTTCCTTTATCAACGAGTTTGTTAAAAGCGTCACTACGGTAAAGCCGATAGGGGTGCTCTAT
GACAATAAAGAGCTCTGTGAGAAGGTGCGTGCAGCATGTAGTGGACAATTAGAGAGCGTCTATAATGAGAGAATAAGTAG
TAAGGAAAAGCGACACAACAAACTTGGCCAGATCTATACAGATACCTTTGCTCAACTGTCTGACGATGGCTATGCTGAGT
CCGAGGTCTTATTCTTCATTAAGAAGCTCGAAAAAGAAATCGTGAGAAAGAACGTTTTGGAGGAGGGCATAAGACCAGAC
GGGAGGAGCCTGACAGAAATACGTCCAATCAGTATCGCTCTGGATTATCTTCCTGGCACTCATGGTTCTGCTCTTTTCAC
TCGTGGTGGGACACAGAGTTTAGTCGTAGCTACGCTGGGGTCTTATCAAGATGAGCAAGTTATGGATGATATCGATGGTG
AGAGAAGGGAGTCCGTACTTTTGCACTATAACTTTTTGCCGTACGCGGTTGGTGAAGTTGGTGCCCTGCGCGCACCAGGA
AGACGGGAAATCGGGCATGGACGCTTAGCCCTAAAAGCGGTGAAGGCTGTTCTGCCAGGTAAAGAGGTTTTCCCTTATAC
TTTACGTTTGGTCTCTGAAATTACAGAATCTGACGGTTCTTCTTCTATGGCAACAGTATGTGGGTCATCTTTGGCTCTAA
TGGATACTGGAGTTCCTATTACGAAGCATGTAGCCGGAATAGCTATGGGCTTGATAACTGATGGAGTAAGGTCTGCCGTT
CTGAGTGACATCTCGGGTGATGAAGACATGCTCGGAGATATGGACTTCAAGGTTGCGGGTACACGAAATGGAATTGTCGC
ACTTCAGATGGATATGAAAGTGCGTGGCATAAGTATAGCAACAATTAGGGATGCACTAAATCAGGCACTGGCTGGAAGGT
TGCACATCCTTGAAAAGATGGAGCATGTAATTGCTAAGCCCCGGGAAAGTCTAAAGGATTCGGCCCCAAAGATACTGTGC
TACAAGATTGATAAGGATGTCGTACATAAGGTGATAGGCAGTGGTGGGAAAACTATTAGGGGGATTTCGTCTGATACCTC
TGCAAAGATTGATATAGATCAGAATAACTACGTTTATATAATGGCTGACACCGAGGAGGCATTGATGGAAGCAAAAACTA
GGGTTGATATGGCTTCAGGTGCTTCTGATTCCAATGTCCCGCAGCTTAAAATTGGTGAACTATATGACGGAAAAATAGTG
AGCGTAGTTGACTTTGGCTTGTTCGTTGTTCTTCCAAATAAGCAGGAGGGATTGGTCCATATTAGTGAAATTAGTAAGAA
CAGGGTTAATGATATACGCGCAGACTACACTGAAGGACAGGCAGTTACGGTGCGGATCAAGGATATAGGTTCGGATGGAA
AAATTAAGCTGACTATGCGCATTGATGAGGATAGAGTAGGTAGTGGTGGAAGTAGCTCCTCACCTAAGAAGCGTTTTGGA
GCCCATCCTAGAAAAAATGGTAAGGATAACCGATCCAATAACAGTGAGAGGGGTTTCAACGAGAGGTCCGGCTCTGCTGA
AGGTTCGTCTATTAGTAGAAAGCGATTTTTCTAA

Upstream 100 bases:

>100_bases
GATCATAAGCAAGAGACGTAGGCTACTCAATTATCTAGCAAAGAATGATTGTCAGGGGTGTAAGAATTTGATGGAAATGC
TTGATATAAGAAAGTAGATA

Downstream 100 bases:

>100_bases
ATCTGGGAGCGTTTTATCTCTAAGAAGCCGGATTTAGCTGGTTTCCACCTTTGTTCGATAACTTCGACGTCTTTTGCCTC
TTGTGATAGCTGTGATCTTG

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 757; Mature: 757

Protein sequence:

>757_residues
MFEIKESSMEWEGKSLHIKTGEVARQAAGAACVSYGGTVVLAVVTLQKDTAASKKTSDLSGLALVTNFLAKSYALGRIPN
GFFKREGKLSEREVLASRVVDRAVRPLIEENLVNEVNIVCKLLAHGNRKVLPEIPALIAASAALQLSGIPFSGPVIGVDV
DMRGSEVTCNEIRETEGGLELFVACTEESVVMVEAEASEASEEEVVNALSEALKSAKPVFSFINEFVKSVTTVKPIGVLY
DNKELCEKVRAACSGQLESVYNERISSKEKRHNKLGQIYTDTFAQLSDDGYAESEVLFFIKKLEKEIVRKNVLEEGIRPD
GRSLTEIRPISIALDYLPGTHGSALFTRGGTQSLVVATLGSYQDEQVMDDIDGERRESVLLHYNFLPYAVGEVGALRAPG
RREIGHGRLALKAVKAVLPGKEVFPYTLRLVSEITESDGSSSMATVCGSSLALMDTGVPITKHVAGIAMGLITDGVRSAV
LSDISGDEDMLGDMDFKVAGTRNGIVALQMDMKVRGISIATIRDALNQALAGRLHILEKMEHVIAKPRESLKDSAPKILC
YKIDKDVVHKVIGSGGKTIRGISSDTSAKIDIDQNNYVYIMADTEEALMEAKTRVDMASGASDSNVPQLKIGELYDGKIV
SVVDFGLFVVLPNKQEGLVHISEISKNRVNDIRADYTEGQAVTVRIKDIGSDGKIKLTMRIDEDRVGSGGSSSSPKKRFG
AHPRKNGKDNRSNNSERGFNERSGSAEGSSISRKRFF

Sequences:

>Translated_757_residues
MFEIKESSMEWEGKSLHIKTGEVARQAAGAACVSYGGTVVLAVVTLQKDTAASKKTSDLSGLALVTNFLAKSYALGRIPN
GFFKREGKLSEREVLASRVVDRAVRPLIEENLVNEVNIVCKLLAHGNRKVLPEIPALIAASAALQLSGIPFSGPVIGVDV
DMRGSEVTCNEIRETEGGLELFVACTEESVVMVEAEASEASEEEVVNALSEALKSAKPVFSFINEFVKSVTTVKPIGVLY
DNKELCEKVRAACSGQLESVYNERISSKEKRHNKLGQIYTDTFAQLSDDGYAESEVLFFIKKLEKEIVRKNVLEEGIRPD
GRSLTEIRPISIALDYLPGTHGSALFTRGGTQSLVVATLGSYQDEQVMDDIDGERRESVLLHYNFLPYAVGEVGALRAPG
RREIGHGRLALKAVKAVLPGKEVFPYTLRLVSEITESDGSSSMATVCGSSLALMDTGVPITKHVAGIAMGLITDGVRSAV
LSDISGDEDMLGDMDFKVAGTRNGIVALQMDMKVRGISIATIRDALNQALAGRLHILEKMEHVIAKPRESLKDSAPKILC
YKIDKDVVHKVIGSGGKTIRGISSDTSAKIDIDQNNYVYIMADTEEALMEAKTRVDMASGASDSNVPQLKIGELYDGKIV
SVVDFGLFVVLPNKQEGLVHISEISKNRVNDIRADYTEGQAVTVRIKDIGSDGKIKLTMRIDEDRVGSGGSSSSPKKRFG
AHPRKNGKDNRSNNSERGFNERSGSAEGSSISRKRFF
>Mature_757_residues
MFEIKESSMEWEGKSLHIKTGEVARQAAGAACVSYGGTVVLAVVTLQKDTAASKKTSDLSGLALVTNFLAKSYALGRIPN
GFFKREGKLSEREVLASRVVDRAVRPLIEENLVNEVNIVCKLLAHGNRKVLPEIPALIAASAALQLSGIPFSGPVIGVDV
DMRGSEVTCNEIRETEGGLELFVACTEESVVMVEAEASEASEEEVVNALSEALKSAKPVFSFINEFVKSVTTVKPIGVLY
DNKELCEKVRAACSGQLESVYNERISSKEKRHNKLGQIYTDTFAQLSDDGYAESEVLFFIKKLEKEIVRKNVLEEGIRPD
GRSLTEIRPISIALDYLPGTHGSALFTRGGTQSLVVATLGSYQDEQVMDDIDGERRESVLLHYNFLPYAVGEVGALRAPG
RREIGHGRLALKAVKAVLPGKEVFPYTLRLVSEITESDGSSSMATVCGSSLALMDTGVPITKHVAGIAMGLITDGVRSAV
LSDISGDEDMLGDMDFKVAGTRNGIVALQMDMKVRGISIATIRDALNQALAGRLHILEKMEHVIAKPRESLKDSAPKILC
YKIDKDVVHKVIGSGGKTIRGISSDTSAKIDIDQNNYVYIMADTEEALMEAKTRVDMASGASDSNVPQLKIGELYDGKIV
SVVDFGLFVVLPNKQEGLVHISEISKNRVNDIRADYTEGQAVTVRIKDIGSDGKIKLTMRIDEDRVGSGGSSSSPKKRFG
AHPRKNGKDNRSNNSERGFNERSGSAEGSSISRKRFF

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=716, Percent_Identity=35.0558659217877, Blast_Score=365, Evalue=1e-100,
Organism=Escherichia coli, GI145693187, Length=705, Percent_Identity=43.9716312056738, Blast_Score=533, Evalue=1e-152,
Organism=Escherichia coli, GI157672248, Length=209, Percent_Identity=25.3588516746411, Blast_Score=63, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI115534063, Length=672, Percent_Identity=33.4821428571429, Blast_Score=330, Evalue=2e-90,
Organism=Drosophila melanogaster, GI281362905, Length=696, Percent_Identity=32.6149425287356, Blast_Score=355, Evalue=8e-98,
Organism=Drosophila melanogaster, GI24651641, Length=696, Percent_Identity=32.6149425287356, Blast_Score=355, Evalue=8e-98,
Organism=Drosophila melanogaster, GI24651643, Length=696, Percent_Identity=32.6149425287356, Blast_Score=355, Evalue=8e-98,
Organism=Drosophila melanogaster, GI161079377, Length=637, Percent_Identity=33.1240188383046, Blast_Score=337, Evalue=2e-92,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_NEOSM (Q2GEY8)

Other databases:

- EMBL:   CP000237
- RefSeq:   YP_505958.1
- ProteinModelPortal:   Q2GEY8
- SMR:   Q2GEY8
- STRING:   Q2GEY8
- GeneID:   3931805
- GenomeReviews:   CP000237_GR
- KEGG:   nse:NSE_0057
- TIGR:   NSE_0057
- eggNOG:   COG1185
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- ProtClustDB:   PRK11824
- BioCyc:   NSEN222891:NSE_0057-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF54814 KH_prok; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 82012; Mature: 82012

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEIKESSMEWEGKSLHIKTGEVARQAAGAACVSYGGTVVLAVVTLQKDTAASKKTSDLS
CCCCCCCCCCCCCCEEEEECHHHHHHHHCHHHHHCCCEEEEEEEEECCCCCCCCCCCCCH
GLALVTNFLAKSYALGRIPNGFFKREGKLSEREVLASRVVDRAVRPLIEENLVNEVNIVC
HHHHHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLLAHGNRKVLPEIPALIAASAALQLSGIPFSGPVIGVDVDMRGSEVTCNEIRETEGGLE
HHHHCCCCCCCHHHHHHHHHHHHHEECCCCCCCCEEEEEECCCCCCCCHHHHHCCCCCEE
LFVACTEESVVMVEAEASEASEEEVVNALSEALKSAKPVFSFINEFVKSVTTVKPIGVLY
EEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEE
DNKELCEKVRAACSGQLESVYNERISSKEKRHNKLGQIYTDTFAQLSDDGYAESEVLFFI
CCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
KKLEKEIVRKNVLEEGIRPDGRSLTEIRPISIALDYLPGTHGSALFTRGGTQSLVVATLG
HHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCCCEEEECCCCCEEEEEECC
SYQDEQVMDDIDGERRESVLLHYNFLPYAVGEVGALRAPGRREIGHGRLALKAVKAVLPG
CCCHHHHHHHCCCCCCCEEEEEECCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
KEVFPYTLRLVSEITESDGSSSMATVCGSSLALMDTGVPITKHVAGIAMGLITDGVRSAV
CCCHHHHHHHHHHHHHCCCCCHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
LSDISGDEDMLGDMDFKVAGTRNGIVALQMDMKVRGISIATIRDALNQALAGRLHILEKM
HHHCCCCHHHHCCCCEEEECCCCCEEEEEECCEECCEEHHHHHHHHHHHHHHHHHHHHHH
EHVIAKPRESLKDSAPKILCYKIDKDVVHKVIGSGGKTIRGISSDTSAKIDIDQNNYVYI
HHHHHCCHHHHHCCCCEEEEEECCHHHHHHHHCCCCCEEEECCCCCCEEEEECCCCEEEE
MADTEEALMEAKTRVDMASGASDSNVPQLKIGELYDGKIVSVVDFGLFVVLPNKQEGLVH
EECCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCEEEEEECCEEEEECCCCCCCEE
ISEISKNRVNDIRADYTEGQAVTVRIKDIGSDGKIKLTMRIDEDRVGSGGSSSSPKKRFG
HHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCHHHHC
AHPRKNGKDNRSNNSERGFNERSGSAEGSSISRKRFF
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCC
>Mature Secondary Structure
MFEIKESSMEWEGKSLHIKTGEVARQAAGAACVSYGGTVVLAVVTLQKDTAASKKTSDLS
CCCCCCCCCCCCCCEEEEECHHHHHHHHCHHHHHCCCEEEEEEEEECCCCCCCCCCCCCH
GLALVTNFLAKSYALGRIPNGFFKREGKLSEREVLASRVVDRAVRPLIEENLVNEVNIVC
HHHHHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLLAHGNRKVLPEIPALIAASAALQLSGIPFSGPVIGVDVDMRGSEVTCNEIRETEGGLE
HHHHCCCCCCCHHHHHHHHHHHHHEECCCCCCCCEEEEEECCCCCCCCHHHHHCCCCCEE
LFVACTEESVVMVEAEASEASEEEVVNALSEALKSAKPVFSFINEFVKSVTTVKPIGVLY
EEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEE
DNKELCEKVRAACSGQLESVYNERISSKEKRHNKLGQIYTDTFAQLSDDGYAESEVLFFI
CCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
KKLEKEIVRKNVLEEGIRPDGRSLTEIRPISIALDYLPGTHGSALFTRGGTQSLVVATLG
HHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCCCEEEECCCCCEEEEEECC
SYQDEQVMDDIDGERRESVLLHYNFLPYAVGEVGALRAPGRREIGHGRLALKAVKAVLPG
CCCHHHHHHHCCCCCCCEEEEEECCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
KEVFPYTLRLVSEITESDGSSSMATVCGSSLALMDTGVPITKHVAGIAMGLITDGVRSAV
CCCHHHHHHHHHHHHHCCCCCHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
LSDISGDEDMLGDMDFKVAGTRNGIVALQMDMKVRGISIATIRDALNQALAGRLHILEKM
HHHCCCCHHHHCCCCEEEECCCCCEEEEEECCEECCEEHHHHHHHHHHHHHHHHHHHHHH
EHVIAKPRESLKDSAPKILCYKIDKDVVHKVIGSGGKTIRGISSDTSAKIDIDQNNYVYI
HHHHHCCHHHHHCCCCEEEEEECCHHHHHHHHCCCCCEEEECCCCCCEEEEECCCCEEEE
MADTEEALMEAKTRVDMASGASDSNVPQLKIGELYDGKIVSVVDFGLFVVLPNKQEGLVH
EECCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCEEEEEECCEEEEECCCCCCCEE
ISEISKNRVNDIRADYTEGQAVTVRIKDIGSDGKIKLTMRIDEDRVGSGGSSSSPKKRFG
HHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCHHHHC
AHPRKNGKDNRSNNSERGFNERSGSAEGSSISRKRFF
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA