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The map label for this gene is ispF

Identifier: 88607925

GI number: 88607925

Start: 1345210

End: 1345683

Strand: Reverse

Name: ispF

Synonym: APH_1276

Alternate gene names: 88607925

Gene position: 1345683-1345210 (Counterclockwise)

Preceding gene: 88607380

Following gene: 88607247

Centisome position: 91.46

GC content: 41.98

Gene sequence:

>474_bases
ATGGGTTTTGATGTACATAGATTTACCGAGTCAAGAGAGGAAGGGCAGTACATACCCATATGTGGAATAAAGGTAGCGCA
TACACAGGGAATAGTTGCGCATTCGGATGGGGATGTTGCACTTCATGCACTTACAGATGCATTATTAGGCTGCATGGGAG
AGGGGAGTATAGGGCAGCATTTTCCTAATAGTGATCCTCAATGGAAGAATGCAAGCTCATCTTATTTCTTGTTGGAAGCA
CAAAAAAAGGCTGCCGCAAAATCTTATGCGATACTAAATTTTGACGTGACTATAGTGTGTGAGCAACCAAAAATAATGCC
CCATGTACCTAAAATGAAAGAGTTTCTGTCTAAGCTGTTAGATGTTGATGTATCACAAATGAACATTAAGGCAGTAACGA
CGGAAAAGCTTGGGTTTTTGGGTCGCGGTGAAGGTATTGCAGCACATGCCGTGTTGATGTGTTATAGGGTGTAA

Upstream 100 bases:

>100_bases
AGAGCGGTGTAAATTGCTTATTGAGTCTGTATGATTTATACCGGCGGGGTGTTTTGTCATATTGTAAGTGGGGTACTTAG
GGATGCCGTTCAGAGTGGGA

Downstream 100 bases:

>100_bases
CTCAAAACCAGCTCTTAATCTTTGGAGTTCTATAGGCACAACTTCCTAACGTCTCAGGAAACTACAAGATATCAGTGTAA
TATACCGCAGCTGTATAGAG

Product: 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 157; Mature: 156

Protein sequence:

>157_residues
MGFDVHRFTESREEGQYIPICGIKVAHTQGIVAHSDGDVALHALTDALLGCMGEGSIGQHFPNSDPQWKNASSSYFLLEA
QKKAAAKSYAILNFDVTIVCEQPKIMPHVPKMKEFLSKLLDVDVSQMNIKAVTTEKLGFLGRGEGIAAHAVLMCYRV

Sequences:

>Translated_157_residues
MGFDVHRFTESREEGQYIPICGIKVAHTQGIVAHSDGDVALHALTDALLGCMGEGSIGQHFPNSDPQWKNASSSYFLLEA
QKKAAAKSYAILNFDVTIVCEQPKIMPHVPKMKEFLSKLLDVDVSQMNIKAVTTEKLGFLGRGEGIAAHAVLMCYRV
>Mature_156_residues
GFDVHRFTESREEGQYIPICGIKVAHTQGIVAHSDGDVALHALTDALLGCMGEGSIGQHFPNSDPQWKNASSSYFLLEAQ
KKAAAKSYAILNFDVTIVCEQPKIMPHVPKMKEFLSKLLDVDVSQMNIKAVTTEKLGFLGRGEGIAAHAVLMCYRV

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=155, Percent_Identity=47.0967741935484, Blast_Score=151, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_ANAPZ (Q2GIK8)

Other databases:

- EMBL:   CP000235
- RefSeq:   YP_505793.1
- ProteinModelPortal:   Q2GIK8
- SMR:   Q2GIK8
- STRING:   Q2GIK8
- GeneID:   3930761
- GenomeReviews:   CP000235_GR
- KEGG:   aph:APH_1276
- NMPDR:   fig|212042.5.peg.1223
- TIGR:   APH_1276
- eggNOG:   COG0245
- HOGENOM:   HBG630070
- PhylomeDB:   Q2GIK8
- ProtClustDB:   CLSK749413
- BioCyc:   APHA212042:APH_1276-MONOMER
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 17178; Mature: 17047

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFDVHRFTESREEGQYIPICGIKVAHTQGIVAHSDGDVALHALTDALLGCMGEGSIGQH
CCCCHHHHHCHHCCCCEEEEECEEEECCCCEEEECCCCEEHHHHHHHHHHHCCCCCCCCC
FPNSDPQWKNASSSYFLLEAQKKAAAKSYAILNFDVTIVCEQPKIMPHVPKMKEFLSKLL
CCCCCCCCCCCCCCEEEEECCHHHHHCEEEEEEEEEEEEECCCCCCCCCHHHHHHHHHHH
DVDVSQMNIKAVTTEKLGFLGRGEGIAAHAVLMCYRV
CCCCCCEEEEEEEHHHHCCCCCCCCHHHHHHEEEECC
>Mature Secondary Structure 
GFDVHRFTESREEGQYIPICGIKVAHTQGIVAHSDGDVALHALTDALLGCMGEGSIGQH
CCCHHHHHCHHCCCCEEEEECEEEECCCCEEEECCCCEEHHHHHHHHHHHCCCCCCCCC
FPNSDPQWKNASSSYFLLEAQKKAAAKSYAILNFDVTIVCEQPKIMPHVPKMKEFLSKLL
CCCCCCCCCCCCCCEEEEECCHHHHHCEEEEEEEEEEEEECCCCCCCCCHHHHHHHHHHH
DVDVSQMNIKAVTTEKLGFLGRGEGIAAHAVLMCYRV
CCCCCCEEEEEEEHHHHCCCCCCCCHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA