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The map label for this gene is ispD [H]

Identifier: 88607380

GI number: 88607380

Start: 1345938

End: 1346645

Strand: Reverse

Name: ispD [H]

Synonym: APH_1277

Alternate gene names: 88607380

Gene position: 1346645-1345938 (Counterclockwise)

Preceding gene: 88607251

Following gene: 88607925

Centisome position: 91.53

GC content: 41.81

Gene sequence:

>708_bases
ATGCCTCTTTTCGTTGTACTTATAGTAGCTGCTGGAGAAAGCAGGAGATTGGGAAATAAGAACCAACTGAAGCAATATCT
CAGGATTCAACGTCATTCTGTGCTTGCGCATACCATCACTCAGATGGTTACTTCATTTGTACATTCTGTGAAGGTTGTGA
TCGGACAGGGGCAGGATGCTTTCTACGAACATTCCCTCTTATCTCTTCCTAAAAAGGTGACGGATTTGCTTATACCACCA
GTATATGGTGGAGCTCGCAGACAAGATTCTGTGCGCATAGGTTTGGAAAGCATTGAACACATTAATCCAGAATTTGTAAT
AATACATGATGCCTGTCGCCCTTTTGCTAGTTTGCCAAGTGCTGACGTTATAACTTACCATCTACGGGATCATGCTGGGA
TTGTACCTGTTTTGCAGCCTAGCGATACCATAAGTCGGGTTGAAAACTCAGTTATTATGGAGGAAATAGCGAAGGATTCC
GTTAGAATAGTTCAGACACCTCAAATATACAGGTACAAGGATATTTTGAGATGTCACAAAGAAGTTTACAGCACAGATCC
GGAGAAATATTTCTCAGATGAGTCATCAGTAATGGTAGCGTGTGGGATGTCAGTTATCACTACAGAGGGAGATTATAACA
ACTTTAAGATTACTACCGCCGAGGATGTTACTAGGGCTGAGTTGTATATGCAACACATGGGTAGTTAG

Upstream 100 bases:

>100_bases
AAGTTGTATAACGCGCTACTAGCCGGCTTTATATGCATATCAAAGTGTAGTATGTTGCTATAAATTGCCAGATTATCTTT
TGTTTGCTTGGAGAACTATG

Downstream 100 bases:

>100_bases
AAGTGTGCTAGTTTTGATAGGTTGTGGGGCCTTTAAATGGTGTGATTTTATTGTTCTGCTATGCTACCACGACTTGGATT
TTGTGCATCATGTGTAATAA

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 235; Mature: 234

Protein sequence:

>235_residues
MPLFVVLIVAAGESRRLGNKNQLKQYLRIQRHSVLAHTITQMVTSFVHSVKVVIGQGQDAFYEHSLLSLPKKVTDLLIPP
VYGGARRQDSVRIGLESIEHINPEFVIIHDACRPFASLPSADVITYHLRDHAGIVPVLQPSDTISRVENSVIMEEIAKDS
VRIVQTPQIYRYKDILRCHKEVYSTDPEKYFSDESSVMVACGMSVITTEGDYNNFKITTAEDVTRAELYMQHMGS

Sequences:

>Translated_235_residues
MPLFVVLIVAAGESRRLGNKNQLKQYLRIQRHSVLAHTITQMVTSFVHSVKVVIGQGQDAFYEHSLLSLPKKVTDLLIPP
VYGGARRQDSVRIGLESIEHINPEFVIIHDACRPFASLPSADVITYHLRDHAGIVPVLQPSDTISRVENSVIMEEIAKDS
VRIVQTPQIYRYKDILRCHKEVYSTDPEKYFSDESSVMVACGMSVITTEGDYNNFKITTAEDVTRAELYMQHMGS
>Mature_234_residues
PLFVVLIVAAGESRRLGNKNQLKQYLRIQRHSVLAHTITQMVTSFVHSVKVVIGQGQDAFYEHSLLSLPKKVTDLLIPPV
YGGARRQDSVRIGLESIEHINPEFVIIHDACRPFASLPSADVITYHLRDHAGIVPVLQPSDTISRVENSVIMEEIAKDSV
RIVQTPQIYRYKDILRCHKEVYSTDPEKYFSDESSVMVACGMSVITTEGDYNNFKITTAEDVTRAELYMQHMGS

Specific function: Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl- D-erythritol 4-phosphate (MEP) (ispD), and converts 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C- methyl-D-erythri

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the ispF family [H]

Homologues:

Organism=Escherichia coli, GI1789104, Length=230, Percent_Identity=26.5217391304348, Blast_Score=71, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF01128 IspD; PF02542 YgbB [H]

EC number: =2.7.7.60; =4.6.1.12 [H]

Molecular weight: Translated: 26487; Mature: 26356

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLFVVLIVAAGESRRLGNKNQLKQYLRIQRHSVLAHTITQMVTSFVHSVKVVIGQGQDA
CCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHH
FYEHSLLSLPKKVTDLLIPPVYGGARRQDSVRIGLESIEHINPEFVIIHDACRPFASLPS
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHCCCC
ADVITYHLRDHAGIVPVLQPSDTISRVENSVIMEEIAKDSVRIVQTPQIYRYKDILRCHK
CCEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHH
EVYSTDPEKYFSDESSVMVACGMSVITTEGDYNNFKITTAEDVTRAELYMQHMGS
HHHCCCCHHHHCCCCCEEEEECCEEEEECCCCCCEEEEEHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PLFVVLIVAAGESRRLGNKNQLKQYLRIQRHSVLAHTITQMVTSFVHSVKVVIGQGQDA
CEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHH
FYEHSLLSLPKKVTDLLIPPVYGGARRQDSVRIGLESIEHINPEFVIIHDACRPFASLPS
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHCCCC
ADVITYHLRDHAGIVPVLQPSDTISRVENSVIMEEIAKDSVRIVQTPQIYRYKDILRCHK
CCEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHH
EVYSTDPEKYFSDESSVMVACGMSVITTEGDYNNFKITTAEDVTRAELYMQHMGS
HHHCCCCHHHHCCCCCEEEEECCEEEEECCCCCCEEEEEHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA