Click here to switch to the map view.

The map label for this gene is nuoE [H]

Identifier: 88607823

GI number: 88607823

Start: 776053

End: 776568

Strand: Reverse

Name: nuoE [H]

Synonym: APH_0731

Alternate gene names: 88607823

Gene position: 776568-776053 (Counterclockwise)

Preceding gene: 88607271

Following gene: 88606785

Centisome position: 52.78

GC content: 42.25

Gene sequence:

>516_bases
ATGAACTCTGCAGATCAGGAAAGCTTTAGGTTCACTGATGGTAACCTAGAAGAAGCATATAAGTGCATAAGTAGATATCC
TGAAGGTAGGCAAGCGAGTGCAGTTATGCCTTTGCTGCATTTGGTGCAACAGCAAGCTGGAGGGTTTGTTCCTCGTTCTG
CTATTGAGTATATAGCGAAGCTTTTGAGTATGCGTCCTGTGCATGTGCGTGAAGTGGTTGAGTTTTACTCAATGTATAAT
ACTGCTCCTGTAGGCAAATATTTGGTACAAGTATGTAAGACTACTCCGTGTTGGTTACGGCGCAGCGATGATGTTCTCAA
TGCATGTAAGAGGGTGCTATGTGTGCGTGTTGGGGAAACAACTAAAGACAACCTTTTTACTTTAAGAGAAGTAGAATGTC
TAGGTGCGTGCGTGAATGCTCCCGTTGTACAGATAAATGATGACTATTACGAAAATCTAGATGCGGAATCTATGGAGAAG
ATTTTGCTGAAATTAAAGGAGGGCAATGAAGGTTAG

Upstream 100 bases:

>100_bases
CTCACTTGCAGGCCATAGATGCTATGGCGCGTGGACACATGCTTGCGGATTTGCCGGTTATTATAGGCTCTTTGGATATT
GTTTTTGGTGAAATAGATAG

Downstream 100 bases:

>100_bases
AGAGATTGTTTTAGATACGGAAACAACTGGCCTTGATGCTGATGGTGGCGACAGAATAATAGAGATAGGGTGCGTTGAGC
TAGTAGATTATGTTATGACT

Product: NADH dehydrogenase subunit E

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E; NDH-1 subunit E [H]

Number of amino acids: Translated: 171; Mature: 171

Protein sequence:

>171_residues
MNSADQESFRFTDGNLEEAYKCISRYPEGRQASAVMPLLHLVQQQAGGFVPRSAIEYIAKLLSMRPVHVREVVEFYSMYN
TAPVGKYLVQVCKTTPCWLRRSDDVLNACKRVLCVRVGETTKDNLFTLREVECLGACVNAPVVQINDDYYENLDAESMEK
ILLKLKEGNEG

Sequences:

>Translated_171_residues
MNSADQESFRFTDGNLEEAYKCISRYPEGRQASAVMPLLHLVQQQAGGFVPRSAIEYIAKLLSMRPVHVREVVEFYSMYN
TAPVGKYLVQVCKTTPCWLRRSDDVLNACKRVLCVRVGETTKDNLFTLREVECLGACVNAPVVQINDDYYENLDAESMEK
ILLKLKEGNEG
>Mature_171_residues
MNSADQESFRFTDGNLEEAYKCISRYPEGRQASAVMPLLHLVQQQAGGFVPRSAIEYIAKLLSMRPVHVREVVEFYSMYN
TAPVGKYLVQVCKTTPCWLRRSDDVLNACKRVLCVRVGETTKDNLFTLREVECLGACVNAPVVQINDDYYENLDAESMEK
ILLKLKEGNEG

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=160, Percent_Identity=54.375, Blast_Score=181, Evalue=3e-46,
Organism=Escherichia coli, GI1788621, Length=160, Percent_Identity=30.625, Blast_Score=80, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17561328, Length=162, Percent_Identity=49.3827160493827, Blast_Score=168, Evalue=1e-42,
Organism=Drosophila melanogaster, GI18859877, Length=158, Percent_Identity=52.5316455696203, Blast_Score=173, Evalue=5e-44,
Organism=Drosophila melanogaster, GI21355353, Length=158, Percent_Identity=41.1392405063291, Blast_Score=140, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 19362; Mature: 19362

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS01099 COMPLEX1_24K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
4.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSADQESFRFTDGNLEEAYKCISRYPEGRQASAVMPLLHLVQQQAGGFVPRSAIEYIAK
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LLSMRPVHVREVVEFYSMYNTAPVGKYLVQVCKTTPCWLRRSDDVLNACKRVLCVRVGET
HHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHCCCC
TKDNLFTLREVECLGACVNAPVVQINDDYYENLDAESMEKILLKLKEGNEG
CCCHHHHHHHHHHHHHHHCCCEEEECCHHHHCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNSADQESFRFTDGNLEEAYKCISRYPEGRQASAVMPLLHLVQQQAGGFVPRSAIEYIAK
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LLSMRPVHVREVVEFYSMYNTAPVGKYLVQVCKTTPCWLRRSDDVLNACKRVLCVRVGET
HHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHCCCC
TKDNLFTLREVECLGACVNAPVVQINDDYYENLDAESMEKILLKLKEGNEG
CCCHHHHHHHHHHHHHHHCCCEEEECCHHHHCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]