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The map label for this gene is grpE [H]

Identifier: 88607566

GI number: 88607566

Start: 32641

End: 33276

Strand: Reverse

Name: grpE [H]

Synonym: APH_0036

Alternate gene names: 88607566

Gene position: 33276-32641 (Counterclockwise)

Preceding gene: 88607103

Following gene: 88607522

Centisome position: 2.26

GC content: 44.18

Gene sequence:

>636_bases
ATGACAGAAGAGAAACGCGTAAATTCTAAGCCTGAATCTGATGACGTGGTCGGCGCGTGTGCTGGCGCTGCGGAATCGCA
TAAGGAAAAGAGTTCAAAGGTTGAATCTGGTAAAAATGCAAAACCGGAAGTCGGTAAGTTTGCAGCTAGTGTTGGAAAGA
CACAGAGTAGGATAAGTGCAGACCTCGCTGAGGTAGAAAAGTTGCGTGCAGAGGCAGAGCTACTTCGCAATCAACTTCGC
CTTGCAGTGGCAGATAGCAAAAACTTGGAGCGCTTAATGAACAGAGAGATTAGTGATGCTAAAGTTTTCTCAATCTCTGG
TTTTGTACGCGATCTGGTACCTTCATTTGATAATCTAGAAGCATCTTTGAAGAATCTCAATGCTGATGATAGCATTCATG
CTGGAATAGAGATGACATGGAATAGCTTAATGGCGGTGTTGAACAGCCATGGTGTCACGAGGATATGTCCTGTTGGTGAG
GCGTTTGACACAAAGTTTCACACAGCCGTTACACAGGTTATAGATAATGATAAGCCTGCGGGTACTATAATTGAGGTGGT
TCAAGCTGGCTATGTGCTTAATGGTAAAGTGTTAAGACCTGCTTCTGTTGTGGTATCAGCACAGGCTAATAACTAG

Upstream 100 bases:

>100_bases
GAATCTCTCGTCAGGCACCATCGGTAAATGCTTTTGCTCTTGAAATTTACTTATAAAGCTACTATATACCTAAATGTTCT
TGTGTAGAAGTTTTTGTGCC

Downstream 100 bases:

>100_bases
TTCACCATGATTTTTTCTGCCGTACAGCCCAGTGGTGGCTCGTTACATCTGGGCAATTATCTTGGAGCAGTGAAGAAGTG
GGTTGCGTTGCAGGACGCCG

Product: co-chaperone GrpE

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MTEEKRVNSKPESDDVVGACAGAAESHKEKSSKVESGKNAKPEVGKFAASVGKTQSRISADLAEVEKLRAEAELLRNQLR
LAVADSKNLERLMNREISDAKVFSISGFVRDLVPSFDNLEASLKNLNADDSIHAGIEMTWNSLMAVLNSHGVTRICPVGE
AFDTKFHTAVTQVIDNDKPAGTIIEVVQAGYVLNGKVLRPASVVVSAQANN

Sequences:

>Translated_211_residues
MTEEKRVNSKPESDDVVGACAGAAESHKEKSSKVESGKNAKPEVGKFAASVGKTQSRISADLAEVEKLRAEAELLRNQLR
LAVADSKNLERLMNREISDAKVFSISGFVRDLVPSFDNLEASLKNLNADDSIHAGIEMTWNSLMAVLNSHGVTRICPVGE
AFDTKFHTAVTQVIDNDKPAGTIIEVVQAGYVLNGKVLRPASVVVSAQANN
>Mature_210_residues
TEEKRVNSKPESDDVVGACAGAAESHKEKSSKVESGKNAKPEVGKFAASVGKTQSRISADLAEVEKLRAEAELLRNQLRL
AVADSKNLERLMNREISDAKVFSISGFVRDLVPSFDNLEASLKNLNADDSIHAGIEMTWNSLMAVLNSHGVTRICPVGEA
FDTKFHTAVTQVIDNDKPAGTIIEVVQAGYVLNGKVLRPASVVVSAQANN

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=191, Percent_Identity=29.3193717277487, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI40255109, Length=161, Percent_Identity=28.5714285714286, Blast_Score=73, Evalue=2e-13,
Organism=Escherichia coli, GI1788967, Length=127, Percent_Identity=32.2834645669291, Blast_Score=72, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17552458, Length=155, Percent_Identity=29.0322580645161, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6324806, Length=188, Percent_Identity=30.3191489361702, Blast_Score=99, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24653432, Length=169, Percent_Identity=33.7278106508876, Blast_Score=92, Evalue=2e-19,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 22661; Mature: 22530

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEEKRVNSKPESDDVVGACAGAAESHKEKSSKVESGKNAKPEVGKFAASVGKTQSRISA
CCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
DLAEVEKLRAEAELLRNQLRLAVADSKNLERLMNREISDAKVFSISGFVRDLVPSFDNLE
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCEEHHHHHHHHHCCCHHHHH
ASLKNLNADDSIHAGIEMTWNSLMAVLNSHGVTRICPVGEAFDTKFHTAVTQVIDNDKPA
HHHHHCCCCCCHHCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCH
GTIIEVVQAGYVLNGKVLRPASVVVSAQANN
HHHHHHHHCCEEECCCEECCCEEEEEECCCC
>Mature Secondary Structure 
TEEKRVNSKPESDDVVGACAGAAESHKEKSSKVESGKNAKPEVGKFAASVGKTQSRISA
CCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
DLAEVEKLRAEAELLRNQLRLAVADSKNLERLMNREISDAKVFSISGFVRDLVPSFDNLE
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCEEHHHHHHHHHCCCHHHHH
ASLKNLNADDSIHAGIEMTWNSLMAVLNSHGVTRICPVGEAFDTKFHTAVTQVIDNDKPA
HHHHHCCCCCCHHCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCH
GTIIEVVQAGYVLNGKVLRPASVVVSAQANN
HHHHHHHHCCEEECCCEECCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA