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The map label for this gene is trpS [H]

Identifier: 88607522

GI number: 88607522

Start: 31639

End: 32634

Strand: Reverse

Name: trpS [H]

Synonym: APH_0035

Alternate gene names: 88607522

Gene position: 32634-31639 (Counterclockwise)

Preceding gene: 88607566

Following gene: 88606808

Centisome position: 2.22

GC content: 44.48

Gene sequence:

>996_bases
ATGATTTTTTCTGCCGTACAGCCCAGTGGTGGCTCGTTACATCTGGGCAATTATCTTGGAGCAGTGAAGAAGTGGGTTGC
GTTGCAGGACGCCGGCAAGTGCGTGTTTTGCATCGTGGACATGCATGCTTTAACCTCGGGAAGTGCAGAAACACTGAGTA
TACGGGCAAATACGCTCAGTTTATTAGCTTCTTATATCGCTTGTGGCATAGATCCTGCAAAAGCGGTAGTTTTTTTACAG
TCGTCTGTTCCTGAACATGCAGAGCTCTGCTGGATACTAGGATGTTTGACGCCCGTGGGGTGGTTGAATAGGATGACACA
GTTCAAGGACAAGAGCCGTTCAGATTCCTACCGGGCTAATTTGGGTCTATACAGTTACCCTGTGCTTATGGCTGCAGACA
TTCTTTTGTATAAAGCGGATTTGGTTCCTGTAGGAAATGATCAAAAACAACACTTGGAGCTTGCACAGAGTATTGCTAGG
ACGTTCAATACTATTTATGGAGTTGACTTTTTTTGCATTCCGGAAGCAATGCCTTTTGATTCCGCCGCTAGGATCATGAG
CTTGAAAACGGGTACAAAGAAGATGAGCAAGTCCGACCCTTCTGACTTCTCTCGTATAAATTTGAGTGATGATAACGATG
TTATAGCCTTGAAGATAAAAAAGGCTACAACCGATTCTGCAACTGGCTTTTGCTATGGAGGACTCAACCAGCGGCCAGAG
GTGAACAATCTCGTAAACATATTTGCAGCACTATCTGATTCAGAACCCAGGGAGGTGTGTGTAAGGTTTACAAACAGTAG
CAATAAAGATTTTAAAGATGCGTTAACGGAGTTACTGATCGAAAGAATATCGCCCATTAGAACCAAGACCAGGGAATTAC
TGGACGATCCTGGTTATCTCAACTCCATACTTTCCTCTGGAAATGAACAAGCTCGGTCAATCGCGGGTAAAAATCTGCGG
GAAGTGAAGGAGATCATAGGTTTGTACAGTGTGTAG

Upstream 100 bases:

>100_bases
TACTATAATTGAGGTGGTTCAAGCTGGCTATGTGCTTAATGGTAAAGTGTTAAGACCTGCTTCTGTTGTGGTATCAGCAC
AGGCTAATAACTAGTTCACC

Downstream 100 bases:

>100_bases
CACGCTCTGTTAGTGATGTCTCTAATAATGCTGTATTTTTCTGTGACATATTAGCTGCTGTGATACTTCGTAACCTACTT
AATTCAGCAGCACAGTAATT

Product: tryptophanyl-tRNA synthetase

Products: NA

Alternate protein names: Tryptophan--tRNA ligase; TrpRS [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MIFSAVQPSGGSLHLGNYLGAVKKWVALQDAGKCVFCIVDMHALTSGSAETLSIRANTLSLLASYIACGIDPAKAVVFLQ
SSVPEHAELCWILGCLTPVGWLNRMTQFKDKSRSDSYRANLGLYSYPVLMAADILLYKADLVPVGNDQKQHLELAQSIAR
TFNTIYGVDFFCIPEAMPFDSAARIMSLKTGTKKMSKSDPSDFSRINLSDDNDVIALKIKKATTDSATGFCYGGLNQRPE
VNNLVNIFAALSDSEPREVCVRFTNSSNKDFKDALTELLIERISPIRTKTRELLDDPGYLNSILSSGNEQARSIAGKNLR
EVKEIIGLYSV

Sequences:

>Translated_331_residues
MIFSAVQPSGGSLHLGNYLGAVKKWVALQDAGKCVFCIVDMHALTSGSAETLSIRANTLSLLASYIACGIDPAKAVVFLQ
SSVPEHAELCWILGCLTPVGWLNRMTQFKDKSRSDSYRANLGLYSYPVLMAADILLYKADLVPVGNDQKQHLELAQSIAR
TFNTIYGVDFFCIPEAMPFDSAARIMSLKTGTKKMSKSDPSDFSRINLSDDNDVIALKIKKATTDSATGFCYGGLNQRPE
VNNLVNIFAALSDSEPREVCVRFTNSSNKDFKDALTELLIERISPIRTKTRELLDDPGYLNSILSSGNEQARSIAGKNLR
EVKEIIGLYSV
>Mature_331_residues
MIFSAVQPSGGSLHLGNYLGAVKKWVALQDAGKCVFCIVDMHALTSGSAETLSIRANTLSLLASYIACGIDPAKAVVFLQ
SSVPEHAELCWILGCLTPVGWLNRMTQFKDKSRSDSYRANLGLYSYPVLMAADILLYKADLVPVGNDQKQHLELAQSIAR
TFNTIYGVDFFCIPEAMPFDSAARIMSLKTGTKKMSKSDPSDFSRINLSDDNDVIALKIKKATTDSATGFCYGGLNQRPE
VNNLVNIFAALSDSEPREVCVRFTNSSNKDFKDALTELLIERISPIRTKTRELLDDPGYLNSILSSGNEQARSIAGKNLR
EVKEIIGLYSV

Specific function: Unknown

COG id: COG0180

COG function: function code J; Tryptophanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7710154, Length=328, Percent_Identity=38.719512195122, Blast_Score=239, Evalue=2e-63,
Organism=Homo sapiens, GI41352700, Length=177, Percent_Identity=46.8926553672316, Blast_Score=169, Evalue=3e-42,
Organism=Escherichia coli, GI1789786, Length=329, Percent_Identity=42.8571428571429, Blast_Score=267, Evalue=7e-73,
Organism=Caenorhabditis elegans, GI71982800, Length=333, Percent_Identity=38.7387387387387, Blast_Score=234, Evalue=4e-62,
Organism=Caenorhabditis elegans, GI71982793, Length=333, Percent_Identity=38.7387387387387, Blast_Score=233, Evalue=1e-61,
Organism=Saccharomyces cerevisiae, GI6320474, Length=347, Percent_Identity=41.7867435158501, Blast_Score=262, Evalue=4e-71,
Organism=Drosophila melanogaster, GI24666151, Length=330, Percent_Identity=43.3333333333333, Blast_Score=254, Evalue=7e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002306 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.2 [H]

Molecular weight: Translated: 36253; Mature: 36253

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFSAVQPSGGSLHLGNYLGAVKKWVALQDAGKCVFCIVDMHALTSGSAETLSIRANTLS
CCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHCCCCCCEEEEEHHHHH
LLASYIACGIDPAKAVVFLQSSVPEHAELCWILGCLTPVGWLNRMTQFKDKSRSDSYRAN
HHHHHHHCCCCHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHC
LGLYSYPVLMAADILLYKADLVPVGNDQKQHLELAQSIARTFNTIYGVDFFCIPEAMPFD
CCHHHHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCC
SAARIMSLKTGTKKMSKSDPSDFSRINLSDDNDVIALKIKKATTDSATGFCYGGLNQRPE
HHHHHHHHCCCHHHHCCCCCCCCCEEECCCCCCEEEEEEEECCCCCCCEEEECCCCCCCC
VNNLVNIFAALSDSEPREVCVRFTNSSNKDFKDALTELLIERISPIRTKTRELLDDPGYL
HHHHHHHHHHHCCCCHHHHHEEECCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHCCCHHH
NSILSSGNEQARSIAGKNLREVKEIIGLYSV
HHHHHCCCHHHHHHHCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MIFSAVQPSGGSLHLGNYLGAVKKWVALQDAGKCVFCIVDMHALTSGSAETLSIRANTLS
CCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHCCCCCCEEEEEHHHHH
LLASYIACGIDPAKAVVFLQSSVPEHAELCWILGCLTPVGWLNRMTQFKDKSRSDSYRAN
HHHHHHHCCCCHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHC
LGLYSYPVLMAADILLYKADLVPVGNDQKQHLELAQSIARTFNTIYGVDFFCIPEAMPFD
CCHHHHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCC
SAARIMSLKTGTKKMSKSDPSDFSRINLSDDNDVIALKIKKATTDSATGFCYGGLNQRPE
HHHHHHHHCCCHHHHCCCCCCCCCEEECCCCCCEEEEEEEECCCCCCCEEEECCCCCCCC
VNNLVNIFAALSDSEPREVCVRFTNSSNKDFKDALTELLIERISPIRTKTRELLDDPGYL
HHHHHHHHHHHCCCCHHHHHEEECCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHCCCHHH
NSILSSGNEQARSIAGKNLREVKEIIGLYSV
HHHHHCCCHHHHHHHCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]