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The map label for this gene is alaS [H]

Identifier: 88607554

GI number: 88607554

Start: 1157660

End: 1157944

Strand: Reverse

Name: alaS [H]

Synonym: APH_1096

Alternate gene names: 88607554

Gene position: 1157944-1157660 (Counterclockwise)

Preceding gene: 88607712

Following gene: 88606744

Centisome position: 78.7

GC content: 41.4

Gene sequence:

>285_bases
GTGATTTTTTGTAACTTTTGCAATGATTCCGAGCTTGTCAAGTCAAAAGAAGTAGCGATCTCAACGACAGATGGGAAGAG
AACAACGTTTATAGTAGGGGTAGGGGAGTCTGCAATCAAAAGGATAAAAGCAACAGACATAGTAAAAGCATTACAGCAAA
TCCAAGGCAAAGGGGGAGGAAATGCCAGTATAGCTAGAGCAAGTCTACCAAGCGAATATTCTGCAAAAGCAGCTGAGATC
ATAAGGCAAACTGTCATTGACGCAATTCAAAATAGCGGAGCATAG

Upstream 100 bases:

>100_bases
TGTTCGGATAGTTGAGCAGTACAAGTTGCTTTTGAAGTTTTTGTATCGGTTCAAGTAAAATTCTCACTAAATGGAGAAGG
AGCGTTTTATTATTCCCCAA

Downstream 100 bases:

>100_bases
GTATCCAGACCACTCGGATACCCTGACCCAGGCATTCTTTATCAAACGCTTCATCTAGTGAATCATCGATTGCATCCCCC
AATTTACTGTAGTTACCAAT

Product: alanyl-tRNA synthetase domain-containing protein

Products: NA

Alternate protein names: Alanine--tRNA ligase; AlaRS [H]

Number of amino acids: Translated: 94; Mature: 94

Protein sequence:

>94_residues
MIFCNFCNDSELVKSKEVAISTTDGKRTTFIVGVGESAIKRIKATDIVKALQQIQGKGGGNASIARASLPSEYSAKAAEI
IRQTVIDAIQNSGA

Sequences:

>Translated_94_residues
MIFCNFCNDSELVKSKEVAISTTDGKRTTFIVGVGESAIKRIKATDIVKALQQIQGKGGGNASIARASLPSEYSAKAAEI
IRQTVIDAIQNSGA
>Mature_94_residues
MIFCNFCNDSELVKSKEVAISTTDGKRTTFIVGVGESAIKRIKATDIVKALQQIQGKGGGNASIARASLPSEYSAKAAEI
IRQTVIDAIQNSGA

Specific function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction:alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editin

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002318
- InterPro:   IPR018162
- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR023033
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: =6.1.1.7 [H]

Molecular weight: Translated: 9902; Mature: 9902

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFCNFCNDSELVKSKEVAISTTDGKRTTFIVGVGESAIKRIKATDIVKALQQIQGKGGG
CEEEECCCCHHHHHCCCEEEEECCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCC
NASIARASLPSEYSAKAAEIIRQTVIDAIQNSGA
CCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIFCNFCNDSELVKSKEVAISTTDGKRTTFIVGVGESAIKRIKATDIVKALQQIQGKGGG
CEEEECCCCHHHHHCCCEEEEECCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCC
NASIARASLPSEYSAKAAEIIRQTVIDAIQNSGA
CCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA