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The map label for this gene is dnaN [H]

Identifier: 88607712

GI number: 88607712

Start: 1158212

End: 1159339

Strand: Reverse

Name: dnaN [H]

Synonym: APH_1097

Alternate gene names: 88607712

Gene position: 1159339-1158212 (Counterclockwise)

Preceding gene: 88607131

Following gene: 88607554

Centisome position: 78.8

GC content: 44.59

Gene sequence:

>1128_bases
ATGAGCGGTGAGATAAGTAGAGGGCTTGATTTCAGTATCAGTCGAGAAGATCTGCTACAAGCGCTGAATTATGTTAGCAG
TGTTATCGAGCGGCGTAACAGTATTGCTGTTCTGTCCTGTGTTAGGATAGAAGCTAAGGGCTCAGTTATTGAGTTTACTT
CTACAGATTTAGACATATCAATAGAAGCGTGTGTACAGGCCAATGTTGCTACGGGAGGTGTGGCTGTAGCGTCTGCGCAG
CTGCTGTACGATATAGTTAGAAAGCTTTCTGGTGAAGCGGACGTTAGGTTCAAACTTAATGATGGCAAGCTGCTAGTGGC
GTGTGGAAATGCGAGATTCTATCTTCACGTTATATCTCCGGAGAAGTTTCCTGACATTGAGGGTAGTTCCCTTGATTGCG
GGTTTGATATACCTGCGACTAGTCTTGCGGAGCTTTTGAATAAGACCAAGTTTTCCATGTCTGTTGAGGAAAGTAGGTAT
AATCTCCGGGGTGTGTATTTCCATGTTGATGACGAAATGCTATGTTGTGTGGCAACAGATGGTCATCGTTTATCGCTGGC
GAAGATAAGTAAGCCTGAGGGTTTGCATTCTGGTATTGCGGTTATAATCCCTCGTAAGACCGTGAATGAGGTTTTGAAGA
TCATTGGCCATGGTGGAGGCGATGCGGCGCAAATGGTCTCTGTAGGAATATCTAATAGGAAAATATGCTTTAGGTATAAG
GGCTACTTTATGGTGTCCAAGGTGTTGGACGGTACGTTTCCAGACTACGCAGCCATTATCCCAACGGAATGCGGTAATAG
TGTATCAATTGACGCGAGCGTATTGCAGAGTGCAGTAGACCGTGTATCAGTAGTAGTATTCGACAAGGTAAAGGCAATCA
AGCTCTCGTTTACAGAGGGTAAGTTGGTATTAAGTTCAGCGTCGTCAGATCAGGGAGATGCAAGCGAAGAGTTGTCTGTA
GACTATGAAGGAGATGAAGTTGTAATAGGATTAAATGCTAGATACCTAATGGAGGTATTGGGAAGCATCAAGGGTGTGTG
CGAAATAGTATTTAGCGACGGTAGTGCGGCGGTACTGGTTAAGGATGGCGAATATGGTATGCACATCGTTATGCCTATGA
GAATATAG

Upstream 100 bases:

>100_bases
TATCAGTGGGTGTATTACGCCTTCTGTTGTGCTTGTCGTATTGACATTATTCTGGATTATGTTTAGAATGCGCAACTTTG
GGAAGTAGTCTTAGGGGCTT

Downstream 100 bases:

>100_bases
GTGTTCAGAGATCGTCACGTTTAATTTTCCTAAGTTCTTCTCAAAGAGAAGAGTTGTGTGCTTATGAGACTACAGTCTCT
CTTAAGTATGCTCTTATCTT

Product: DNA polymerase III, beta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MSGEISRGLDFSISREDLLQALNYVSSVIERRNSIAVLSCVRIEAKGSVIEFTSTDLDISIEACVQANVATGGVAVASAQ
LLYDIVRKLSGEADVRFKLNDGKLLVACGNARFYLHVISPEKFPDIEGSSLDCGFDIPATSLAELLNKTKFSMSVEESRY
NLRGVYFHVDDEMLCCVATDGHRLSLAKISKPEGLHSGIAVIIPRKTVNEVLKIIGHGGGDAAQMVSVGISNRKICFRYK
GYFMVSKVLDGTFPDYAAIIPTECGNSVSIDASVLQSAVDRVSVVVFDKVKAIKLSFTEGKLVLSSASSDQGDASEELSV
DYEGDEVVIGLNARYLMEVLGSIKGVCEIVFSDGSAAVLVKDGEYGMHIVMPMRI

Sequences:

>Translated_375_residues
MSGEISRGLDFSISREDLLQALNYVSSVIERRNSIAVLSCVRIEAKGSVIEFTSTDLDISIEACVQANVATGGVAVASAQ
LLYDIVRKLSGEADVRFKLNDGKLLVACGNARFYLHVISPEKFPDIEGSSLDCGFDIPATSLAELLNKTKFSMSVEESRY
NLRGVYFHVDDEMLCCVATDGHRLSLAKISKPEGLHSGIAVIIPRKTVNEVLKIIGHGGGDAAQMVSVGISNRKICFRYK
GYFMVSKVLDGTFPDYAAIIPTECGNSVSIDASVLQSAVDRVSVVVFDKVKAIKLSFTEGKLVLSSASSDQGDASEELSV
DYEGDEVVIGLNARYLMEVLGSIKGVCEIVFSDGSAAVLVKDGEYGMHIVMPMRI
>Mature_374_residues
SGEISRGLDFSISREDLLQALNYVSSVIERRNSIAVLSCVRIEAKGSVIEFTSTDLDISIEACVQANVATGGVAVASAQL
LYDIVRKLSGEADVRFKLNDGKLLVACGNARFYLHVISPEKFPDIEGSSLDCGFDIPATSLAELLNKTKFSMSVEESRYN
LRGVYFHVDDEMLCCVATDGHRLSLAKISKPEGLHSGIAVIIPRKTVNEVLKIIGHGGGDAAQMVSVGISNRKICFRYKG
YFMVSKVLDGTFPDYAAIIPTECGNSVSIDASVLQSAVDRVSVVVFDKVKAIKLSFTEGKLVLSSASSDQGDASEELSVD
YEGDEVVIGLNARYLMEVLGSIKGVCEIVFSDGSAAVLVKDGEYGMHIVMPMRI

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=373, Percent_Identity=29.7587131367292, Blast_Score=190, Evalue=1e-49,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40392; Mature: 40261

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGEISRGLDFSISREDLLQALNYVSSVIERRNSIAVLSCVRIEAKGSVIEFTSTDLDIS
CCCCCCCCCCCEECHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCEEEEECCCCEEE
IEACVQANVATGGVAVASAQLLYDIVRKLSGEADVRFKLNDGKLLVACGNARFYLHVISP
EEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEECCCEEEEEEECC
EKFPDIEGSSLDCGFDIPATSLAELLNKTKFSMSVEESRYNLRGVYFHVDDEMLCCVATD
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEEEEECCCCCEEEEEEEEECCCEEEEEECC
GHRLSLAKISKPEGLHSGIAVIIPRKTVNEVLKIIGHGGGDAAQMVSVGISNRKICFRYK
CCEEEEEECCCCCCCCCCEEEEEEHHHHHHHHHHHCCCCCCHHHHEEECCCCCEEEEEEC
GYFMVSKVLDGTFPDYAAIIPTECGNSVSIDASVLQSAVDRVSVVVFDKVKAIKLSFTEG
CEEEEEHHHCCCCCCCEEEEECCCCCEEEECHHHHHHHHHHEEEEEEECEEEEEEEECCC
KLVLSSASSDQGDASEELSVDYEGDEVVIGLNARYLMEVLGSIKGVCEIVFSDGSAAVLV
EEEEEECCCCCCCCCCCEEEEECCCEEEEECCHHHHHHHHHHHCEEEEEEECCCCEEEEE
KDGEYGMHIVMPMRI
ECCCCCEEEEEEECC
>Mature Secondary Structure 
SGEISRGLDFSISREDLLQALNYVSSVIERRNSIAVLSCVRIEAKGSVIEFTSTDLDIS
CCCCCCCCCCEECHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCEEEEECCCCEEE
IEACVQANVATGGVAVASAQLLYDIVRKLSGEADVRFKLNDGKLLVACGNARFYLHVISP
EEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEECCCEEEEEEECC
EKFPDIEGSSLDCGFDIPATSLAELLNKTKFSMSVEESRYNLRGVYFHVDDEMLCCVATD
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEEEEECCCCCEEEEEEEEECCCEEEEEECC
GHRLSLAKISKPEGLHSGIAVIIPRKTVNEVLKIIGHGGGDAAQMVSVGISNRKICFRYK
CCEEEEEECCCCCCCCCCEEEEEEHHHHHHHHHHHCCCCCCHHHHEEECCCCCEEEEEEC
GYFMVSKVLDGTFPDYAAIIPTECGNSVSIDASVLQSAVDRVSVVVFDKVKAIKLSFTEG
CEEEEEHHHCCCCCCCEEEEECCCCCEEEECHHHHHHHHHHEEEEEEECEEEEEEEECCC
KLVLSSASSDQGDASEELSVDYEGDEVVIGLNARYLMEVLGSIKGVCEIVFSDGSAAVLV
EEEEEECCCCCCCCCCCEEEEECCCEEEEECCHHHHHHHHHHHCEEEEEEECCCCEEEEE
KDGEYGMHIVMPMRI
ECCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9079919; 8226640 [H]