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The map label for this gene is def [H]

Identifier: 88607030

GI number: 88607030

Start: 1435671

End: 1436234

Strand: Reverse

Name: def [H]

Synonym: APH_1372

Alternate gene names: 88607030

Gene position: 1436234-1435671 (Counterclockwise)

Preceding gene: 88606748

Following gene: 88606724

Centisome position: 97.62

GC content: 46.1

Gene sequence:

>564_bases
GTGTCCGTAAGACCTTTGGTTGTTTTGCCTGATAGCCGGTTGTTTCTGTGCTCTGAAGAGGTACGGGAGACTGACTTCTG
TGAGAAGTTCAATTCATTAGTTGAAGACATGTTTGACACCATGTATGCCGAGCAGGGTATAGGTTTAGCCGCAGTTCAGG
TAGGTGTCCATAAACGCATTTTTGTAATTGACTTGGGTCCTAAAACCGAGGAATCTAGCGAAATCGCTGATGATCCTGAT
GGTTATCATTCTACCTGTGGCCCTATGGTTGTTATCAACCCCGAAATTGTAGAAAAATCTGTTGATCTGGTCTCCATGGA
GGAAGGGTGCTTATCTGTGCCTGATCAGAGAGAGTTGGTTGTCCGTCCAGAGCGAATCGTGATGCAGTACACTGACTTAC
ACGGCAAGCGCAAGATCTTAAAGGCGCAGGGGTTACTTTCTAGATGCCTACAGCACGAGATCGACCACTTGAACGGTACC
GTATTTCTCAAGCATATCTCAAAGCTAAAACGCGATCTGGTGATGCAAAAGATGAGAAAAGCTGCCTCGCTTAGGAAGAA
CTAG

Upstream 100 bases:

>100_bases
ACATAGAATGACAAGGCTTTGCCCTCGCAGTACATTGTGATGTACAAGGGTACCAGCGCTCTTGATAATGTAAAGCTTTA
TCGGTTTGAGGAAAAAGGGT

Downstream 100 bases:

>100_bases
TAAATTTGAAATCATAACGCAGCACGAATTTCTCTGAAGATCTTTAGCGCAAATTACAGAGTAGGTGATCTAATGTGGGT
ATAAACATACATTATTGCAT

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 187; Mature: 186

Protein sequence:

>187_residues
MSVRPLVVLPDSRLFLCSEEVRETDFCEKFNSLVEDMFDTMYAEQGIGLAAVQVGVHKRIFVIDLGPKTEESSEIADDPD
GYHSTCGPMVVINPEIVEKSVDLVSMEEGCLSVPDQRELVVRPERIVMQYTDLHGKRKILKAQGLLSRCLQHEIDHLNGT
VFLKHISKLKRDLVMQKMRKAASLRKN

Sequences:

>Translated_187_residues
MSVRPLVVLPDSRLFLCSEEVRETDFCEKFNSLVEDMFDTMYAEQGIGLAAVQVGVHKRIFVIDLGPKTEESSEIADDPD
GYHSTCGPMVVINPEIVEKSVDLVSMEEGCLSVPDQRELVVRPERIVMQYTDLHGKRKILKAQGLLSRCLQHEIDHLNGT
VFLKHISKLKRDLVMQKMRKAASLRKN
>Mature_186_residues
SVRPLVVLPDSRLFLCSEEVRETDFCEKFNSLVEDMFDTMYAEQGIGLAAVQVGVHKRIFVIDLGPKTEESSEIADDPDG
YHSTCGPMVVINPEIVEKSVDLVSMEEGCLSVPDQRELVVRPERIVMQYTDLHGKRKILKAQGLLSRCLQHEIDHLNGTV
FLKHISKLKRDLVMQKMRKAASLRKN

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=185, Percent_Identity=42.1621621621622, Blast_Score=137, Evalue=5e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 21196; Mature: 21065

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVRPLVVLPDSRLFLCSEEVRETDFCEKFNSLVEDMFDTMYAEQGIGLAAVQVGVHKRI
CCCCCEEEECCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEECCCCEE
FVIDLGPKTEESSEIADDPDGYHSTCGPMVVINPEIVEKSVDLVSMEEGCLSVPDQRELV
EEEECCCCCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHCCCCCCCCCCCCEE
VRPERIVMQYTDLHGKRKILKAQGLLSRCLQHEIDHLNGTVFLKHISKLKRDLVMQKMRK
ECHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
AASLRKN
HHHHCCC
>Mature Secondary Structure 
SVRPLVVLPDSRLFLCSEEVRETDFCEKFNSLVEDMFDTMYAEQGIGLAAVQVGVHKRI
CCCCEEEECCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEECCCCEE
FVIDLGPKTEESSEIADDPDGYHSTCGPMVVINPEIVEKSVDLVSMEEGCLSVPDQRELV
EEEECCCCCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHCCCCCCCCCCCCEE
VRPERIVMQYTDLHGKRKILKAQGLLSRCLQHEIDHLNGTVFLKHISKLKRDLVMQKMRK
ECHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
AASLRKN
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA