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The map label for this gene is def [H]

Identifier: 88606748

GI number: 88606748

Start: 1439294

End: 1439569

Strand: Reverse

Name: def [H]

Synonym: APH_1375

Alternate gene names: 88606748

Gene position: 1439569-1439294 (Counterclockwise)

Preceding gene: 88606898

Following gene: 88607030

Centisome position: 97.84

GC content: 46.01

Gene sequence:

>276_bases
ATGATAAACTCCTTTGATCTGGTCTCCATGGAGGAAGGGTGCTTATCTGTGCCTAATCAGAGAGAGTTGGTTGTCCGTCC
AGAGAGAGTCGTGATGCAGTACACTGACTTACACGGCAAGCGCAAGATCTTAAAGGCGCAGGGGTTACTTTCTAGATGCC
TACAGCACGAGATCGACCACTTGAACGGTACCGTATTTATCAAGCACATCTCAAAGCTAAAACGCGATCTGGTGATGCAA
AAAATGAGAAAGGCTGCTTCACTCATGAAGAACTAA

Upstream 100 bases:

>100_bases
GTACTTTTGATAGAAATAATCCATGCGCACTTTCATCTGTACTACCCCGTCTTTATAGCGAAGCACATGTATTTGCCATT
CTATTTCTGGGAGTTAGAGA

Downstream 100 bases:

>100_bases
TCATAACGCAACACGAATTTCTTTGAAGATCCTTAGTACAAATTACAATAATGGTGCTCTTTATGTAAGGCTAGTGATCT
AATGTGGGTATAAACATACA

Product: peptide deformylase, truncation

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 91; Mature: 91

Protein sequence:

>91_residues
MINSFDLVSMEEGCLSVPNQRELVVRPERVVMQYTDLHGKRKILKAQGLLSRCLQHEIDHLNGTVFIKHISKLKRDLVMQ
KMRKAASLMKN

Sequences:

>Translated_91_residues
MINSFDLVSMEEGCLSVPNQRELVVRPERVVMQYTDLHGKRKILKAQGLLSRCLQHEIDHLNGTVFIKHISKLKRDLVMQ
KMRKAASLMKN
>Mature_91_residues
MINSFDLVSMEEGCLSVPNQRELVVRPERVVMQYTDLHGKRKILKAQGLLSRCLQHEIDHLNGTVFIKHISKLKRDLVMQ
KMRKAASLMKN

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=77, Percent_Identity=48.0519480519481, Blast_Score=76, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 10545; Mature: 10545

Theoretical pI: Translated: 10.54; Mature: 10.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
6.6 %Met     (Translated Protein)
8.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
6.6 %Met     (Mature Protein)
8.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINSFDLVSMEEGCLSVPNQRELVVRPERVVMQYTDLHGKRKILKAQGLLSRCLQHEIDH
CCCCCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
LNGTVFIKHISKLKRDLVMQKMRKAASLMKN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MINSFDLVSMEEGCLSVPNQRELVVRPERVVMQYTDLHGKRKILKAQGLLSRCLQHEIDH
CCCCCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
LNGTVFIKHISKLKRDLVMQKMRKAASLMKN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA