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The map label for this gene is gsiB [H]

Identifier: 87200957

GI number: 87200957

Start: 3162340

End: 3163869

Strand: Reverse

Name: gsiB [H]

Synonym: Saro_2945

Alternate gene names: 87200957

Gene position: 3163869-3162340 (Counterclockwise)

Preceding gene: 87200958

Following gene: 87200956

Centisome position: 88.83

GC content: 63.66

Gene sequence:

>1530_bases
ATGGATCTTAGCCGCCGTACACTCATCGGTTCCGGCATCGCCGCTGCTGGCACGCTGGTGCTTCCCGCTTTTGCCGGGCC
CGTGCGCGCCGGCCCCCGACAGGGTGGTTCGATCCGCGTGGCGATGCAGTCCTCGTCCACTGCCGATACGCTCGACCCCG
CCAAGGGCGCGATGGCCACGGACTACGTGCGCCACTTCTGTCTCTATAGCGGTCTCACGCGGATGGAGGCGGACCTGCGC
CCGCGCCCTTTCCTTGCCGAGGCACTAGAGACGGCTGACCGGATAACCTGGCACATAGCCCTCAAGCGCGGCGTCCGCTT
TGCCGATGGTCGTGAACTGACCAGTGCCGATGTCGTGCATTCGTTGCAGCGCCATCTCGATCCCAAGCTGGGCTCGAAGG
TCGCTTCGATCGCCAAGCAGTTCGCCGAAGTGCGCGCCGATGGCCGGCATGGCGTCGTCATCAAGCTGTCCGGGGCCAAC
GCCGACCTTCCCGCGATCCTCTCGCAGTCGCATTTCCTGATCGTGCCTGCTGGAGAGGACAAGCCCAGTGGCAACGGTTG
TGGTCCGTTTCTGCTTGCCGACTTCCGACCCGGCGTGCGCACCGTGGTCACGCGCAATCCCGAATATTGGCGCAACGGCG
AGCCCTATCTCGACCGCATCGAGATCATTGCGATTCCCGACGAGATAAGCCGGGTGAATGCGCTGCTTGCTGGCGACGTG
CAACTTGTCATCGCGGTTCAGCCGCTATCGACGCGGCGGATCGAGCAGTCGTCGCAGCACGGCATTCTGACCACGCCTTC
TCCGCTCTATACTGATCTGGTCATGCGGCAGACCCAGTTGCCAACCGGCAATCCGGACTTCGTGGCGGCGGTCAAGCATC
TGATTGACCGGCCGCTGATCAAGCGCGCGCTGTTTCGGGGTTATGCGACCATCGGAAACGACCAGCCGATTCCTCCCTTC
CACCCCTATTTCAATCCGGCGGTGCCGCAGACGGTGCTCGATCTGGACCGGGCGAAGTGGCACATCGCGCGTTCGGGGCT
GAAGGGCGTGCGGCTGCCGATCTACTGTTCGCCTGCCGCCGCTGGCTCGGTCGACATGGCGTCGGTGTTGCAGGAATATG
GCGCGCAGGCAGGTCTCGAATTTGCGGTGAACCGGATGCCGGCCGACGGCTACTGGTCGACGCACTGGATGAAGCACCCC
ATGAGCTTCGGGAACACCAACCCGCGCCCCACGGCGGACCTCGTGTTCAGTCAGTTCTTCCGTTCGGACGCGGAGTGGAA
CGAGAGCGGGTGGAAGAACCCGCGCTTCGACGAACTGCTGACGCTGGCCCGTGCCGAGGCCGATGAAGCACGTCGCAAGG
AGCTTTATGGCGAGATGCAGCAACTGGTGCATGACCATTGCGGCGTGGCGATTCCGGTATTCATCAACATGCTCGACGGC
CACGACCGGCGACTGAAAGGGATGTCTGCAATCCCCCTCGGCGGCCTGATGGGCTATCGCTTTGCCGAATACGCCTGGTG
GGACGCGTGA

Upstream 100 bases:

>100_bases
TCGCAGATCTGACCTGGCCCGCGATACCGGGACATCTCGGAAAGCCCTGGTTCCTGCCGATGGTAGGCGCCTGGTACCGC
CTTCAGGACTGGCTCCACTG

Downstream 100 bases:

>100_bases
TGGCTGGTTCGGCTACATCCGCGCCGAAATCCGCTCTCGTCGGCGTGCTGCCGCTGATCGGCAAGCGCATCGGTTCTGCG
TTGCTTACGCTGCTGCTGGT

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MDLSRRTLIGSGIAAAGTLVLPAFAGPVRAGPRQGGSIRVAMQSSSTADTLDPAKGAMATDYVRHFCLYSGLTRMEADLR
PRPFLAEALETADRITWHIALKRGVRFADGRELTSADVVHSLQRHLDPKLGSKVASIAKQFAEVRADGRHGVVIKLSGAN
ADLPAILSQSHFLIVPAGEDKPSGNGCGPFLLADFRPGVRTVVTRNPEYWRNGEPYLDRIEIIAIPDEISRVNALLAGDV
QLVIAVQPLSTRRIEQSSQHGILTTPSPLYTDLVMRQTQLPTGNPDFVAAVKHLIDRPLIKRALFRGYATIGNDQPIPPF
HPYFNPAVPQTVLDLDRAKWHIARSGLKGVRLPIYCSPAAAGSVDMASVLQEYGAQAGLEFAVNRMPADGYWSTHWMKHP
MSFGNTNPRPTADLVFSQFFRSDAEWNESGWKNPRFDELLTLARAEADEARRKELYGEMQQLVHDHCGVAIPVFINMLDG
HDRRLKGMSAIPLGGLMGYRFAEYAWWDA

Sequences:

>Translated_509_residues
MDLSRRTLIGSGIAAAGTLVLPAFAGPVRAGPRQGGSIRVAMQSSSTADTLDPAKGAMATDYVRHFCLYSGLTRMEADLR
PRPFLAEALETADRITWHIALKRGVRFADGRELTSADVVHSLQRHLDPKLGSKVASIAKQFAEVRADGRHGVVIKLSGAN
ADLPAILSQSHFLIVPAGEDKPSGNGCGPFLLADFRPGVRTVVTRNPEYWRNGEPYLDRIEIIAIPDEISRVNALLAGDV
QLVIAVQPLSTRRIEQSSQHGILTTPSPLYTDLVMRQTQLPTGNPDFVAAVKHLIDRPLIKRALFRGYATIGNDQPIPPF
HPYFNPAVPQTVLDLDRAKWHIARSGLKGVRLPIYCSPAAAGSVDMASVLQEYGAQAGLEFAVNRMPADGYWSTHWMKHP
MSFGNTNPRPTADLVFSQFFRSDAEWNESGWKNPRFDELLTLARAEADEARRKELYGEMQQLVHDHCGVAIPVFINMLDG
HDRRLKGMSAIPLGGLMGYRFAEYAWWDA
>Mature_509_residues
MDLSRRTLIGSGIAAAGTLVLPAFAGPVRAGPRQGGSIRVAMQSSSTADTLDPAKGAMATDYVRHFCLYSGLTRMEADLR
PRPFLAEALETADRITWHIALKRGVRFADGRELTSADVVHSLQRHLDPKLGSKVASIAKQFAEVRADGRHGVVIKLSGAN
ADLPAILSQSHFLIVPAGEDKPSGNGCGPFLLADFRPGVRTVVTRNPEYWRNGEPYLDRIEIIAIPDEISRVNALLAGDV
QLVIAVQPLSTRRIEQSSQHGILTTPSPLYTDLVMRQTQLPTGNPDFVAAVKHLIDRPLIKRALFRGYATIGNDQPIPPF
HPYFNPAVPQTVLDLDRAKWHIARSGLKGVRLPIYCSPAAAGSVDMASVLQEYGAQAGLEFAVNRMPADGYWSTHWMKHP
MSFGNTNPRPTADLVFSQFFRSDAEWNESGWKNPRFDELLTLARAEADEARRKELYGEMQQLVHDHCGVAIPVFINMLDG
HDRRLKGMSAIPLGGLMGYRFAEYAWWDA

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=515, Percent_Identity=24.6601941747573, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI1789966, Length=463, Percent_Identity=23.9740820734341, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1787495, Length=276, Percent_Identity=24.2753623188406, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1787762, Length=471, Percent_Identity=23.5668789808917, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI1789887, Length=447, Percent_Identity=22.5950782997763, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 56043; Mature: 56043

Theoretical pI: Translated: 8.57; Mature: 8.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLSRRTLIGSGIAAAGTLVLPAFAGPVRAGPRQGGSIRVAMQSSSTADTLDPAKGAMAT
CCCCCCHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHH
DYVRHFCLYSGLTRMEADLRPRPFLAEALETADRITWHIALKRGVRFADGRELTSADVVH
HHHHHHHHHHCHHHHHHCCCCCCHHHHHHHHHHHHEEEEEHHHCCEECCCCCCHHHHHHH
SLQRHLDPKLGSKVASIAKQFAEVRADGRHGVVIKLSGANADLPAILSQSHFLIVPAGED
HHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHCCCCEEEEECCCC
KPSGNGCGPFLLADFRPGVRTVVTRNPEYWRNGEPYLDRIEIIAIPDEISRVNALLAGDV
CCCCCCCCCEEEECCCCCCEEEEECCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHCCCE
QLVIAVQPLSTRRIEQSSQHGILTTPSPLYTDLVMRQTQLPTGNPDFVAAVKHLIDRPLI
EEEEEECCCHHHHHHHCCCCCEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHH
KRALFRGYATIGNDQPIPPFHPYFNPAVPQTVLDLDRAKWHIARSGLKGVRLPIYCSPAA
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCC
AGSVDMASVLQEYGAQAGLEFAVNRMPADGYWSTHWMKHPMSFGNTNPRPTADLVFSQFF
CCCHHHHHHHHHHCHHCCHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHH
RSDAEWNESGWKNPRFDELLTLARAEADEARRKELYGEMQQLVHDHCGVAIPVFINMLDG
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEHHHHHHHHCC
HDRRLKGMSAIPLGGLMGYRFAEYAWWDA
CCHHHCCCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDLSRRTLIGSGIAAAGTLVLPAFAGPVRAGPRQGGSIRVAMQSSSTADTLDPAKGAMAT
CCCCCCHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHH
DYVRHFCLYSGLTRMEADLRPRPFLAEALETADRITWHIALKRGVRFADGRELTSADVVH
HHHHHHHHHHCHHHHHHCCCCCCHHHHHHHHHHHHEEEEEHHHCCEECCCCCCHHHHHHH
SLQRHLDPKLGSKVASIAKQFAEVRADGRHGVVIKLSGANADLPAILSQSHFLIVPAGED
HHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHCCCCEEEEECCCC
KPSGNGCGPFLLADFRPGVRTVVTRNPEYWRNGEPYLDRIEIIAIPDEISRVNALLAGDV
CCCCCCCCCEEEECCCCCCEEEEECCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHCCCE
QLVIAVQPLSTRRIEQSSQHGILTTPSPLYTDLVMRQTQLPTGNPDFVAAVKHLIDRPLI
EEEEEECCCHHHHHHHCCCCCEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHH
KRALFRGYATIGNDQPIPPFHPYFNPAVPQTVLDLDRAKWHIARSGLKGVRLPIYCSPAA
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCC
AGSVDMASVLQEYGAQAGLEFAVNRMPADGYWSTHWMKHPMSFGNTNPRPTADLVFSQFF
CCCHHHHHHHHHHCHHCCHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHH
RSDAEWNESGWKNPRFDELLTLARAEADEARRKELYGEMQQLVHDHCGVAIPVFINMLDG
HCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEHHHHHHHHCC
HDRRLKGMSAIPLGGLMGYRFAEYAWWDA
CCHHHCCCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA