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The map label for this gene is puuB [H]

Identifier: 87200958

GI number: 87200958

Start: 3163869

End: 3165152

Strand: Reverse

Name: puuB [H]

Synonym: Saro_2946

Alternate gene names: 87200958

Gene position: 3165152-3163869 (Counterclockwise)

Preceding gene: 87200959

Following gene: 87200957

Centisome position: 88.87

GC content: 68.38

Gene sequence:

>1284_bases
ATGACGCCGCAGTCCCTCTCCTACTGGCAGGCCAGCACGCCGCCCTTCGCCTCGGGTGAGAAGGCGCGGGTCGAGGGGGA
CTGCGACGTCGCGATCATCGGGGGCGGCTTCACCGGCCTGTCCGCCGCGCTGGAACTGGCGCGCGGCGGGGCGAGCGTGG
TCCTCCTCGAGGCAGGCCGAATTGCCGGAGCCGCTTCGGGTCGCAACGGGGGCCAATGCAACGCGGGCACAGCGCATGAC
TTCGGCGCCCTGGCCTCCGCGCACGGCGTGGACCTTGCCCGCAAATGGTATCTGGCCCATGTCGACGCGGTCGAGACGGT
GGGGCGCATCGCCGCGCGTGAAGGCATCGCTTGCGATTACACCCGCTGCGGCCGGGCCAAGCTTGCGGCAAAGCCGGAAC
ATTTCGCCAAGCTCGAAGCCGCGCACGCGGTGCTGCTGAAGGAAGTCGATCCAAACGTCCTTCTTGTCGGCCCGGGCGAG
CTGTCTTCGGAGATCAACGCCCGCGGCTTCCACGGTGCGCTGGTGCAGACGACGAGCGCGCAGCTCCATCCCGGCAAGCT
TGGCGTGGGCCTTGGCGAAGCCGCCGTCCGCGCCGGCGCCCGGATCTGGGAAAATGCGCCGGTCGAGGCGATGGAACGTG
CGGGCACGGAGTGGAAGCTGTCCACTCCGCGCGGACGGGTAAAGGCACGGCAGGTGCTGGTGGCGACGGGTGGCAACAGT
CCCTATCCGCCCTTCGGATGGTTCCGACGCCGCATCGTGACCGTGGGCAGCTTCGTGATCGCGACCGATCCGCTGCCCGC
CGACCTTGCCGCCCATCTCTTCCCGACCCGCCGCAACTACGTGACCAGCCGGATTATCGGCAACTACTTCCGCCTGACCG
CCGACAACCGCCTCATTTTCGGCGGTCGCGCCCGCTTTGCGCTGTCCAACCCGGCATCCGATGCCAAGTCCGGCCGCATT
CTCGAGGCCAAGCTGCGCGAACTGTTTCCCGAACTCGCCGGGGTCGGTATCGCGGCCTGCTGGGGCGGCGCGATCGACCT
CACCGCCGATCGCCTGCCGCGCGCGGGAGAGCATGACGGGCTGTTCTATGCCATGGGCTACAGCGGCCATGGCGTGCAAA
TGGCGACCGCAATGGGCGAGCACATGGCCCGGGTCATGGGCGGCGACGCTTCTGCCAATCCCTTCGCAGATCTGACCTGG
CCCGCGATACCGGGACATCTCGGAAAGCCCTGGTTCCTGCCGATGGTAGGCGCCTGGTACCGCCTTCAGGACTGGCTCCA
CTGA

Upstream 100 bases:

>100_bases
GTCTTCGTCGGCCGTGGCCACGAACCCTCGAACGCCAATTACCGCGACGTCGAGATCCCGCACATCGGCGCGCTGCCGGC
GGTGGTCGGTCTCTGATCCG

Downstream 100 bases:

>100_bases
TGGATCTTAGCCGCCGTACACTCATCGGTTCCGGCATCGCCGCTGCTGGCACGCTGGTGCTTCCCGCTTTTGCCGGGCCC
GTGCGCGCCGGCCCCCGACA

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MTPQSLSYWQASTPPFASGEKARVEGDCDVAIIGGGFTGLSAALELARGGASVVLLEAGRIAGAASGRNGGQCNAGTAHD
FGALASAHGVDLARKWYLAHVDAVETVGRIAAREGIACDYTRCGRAKLAAKPEHFAKLEAAHAVLLKEVDPNVLLVGPGE
LSSEINARGFHGALVQTTSAQLHPGKLGVGLGEAAVRAGARIWENAPVEAMERAGTEWKLSTPRGRVKARQVLVATGGNS
PYPPFGWFRRRIVTVGSFVIATDPLPADLAAHLFPTRRNYVTSRIIGNYFRLTADNRLIFGGRARFALSNPASDAKSGRI
LEAKLRELFPELAGVGIAACWGGAIDLTADRLPRAGEHDGLFYAMGYSGHGVQMATAMGEHMARVMGGDASANPFADLTW
PAIPGHLGKPWFLPMVGAWYRLQDWLH

Sequences:

>Translated_427_residues
MTPQSLSYWQASTPPFASGEKARVEGDCDVAIIGGGFTGLSAALELARGGASVVLLEAGRIAGAASGRNGGQCNAGTAHD
FGALASAHGVDLARKWYLAHVDAVETVGRIAAREGIACDYTRCGRAKLAAKPEHFAKLEAAHAVLLKEVDPNVLLVGPGE
LSSEINARGFHGALVQTTSAQLHPGKLGVGLGEAAVRAGARIWENAPVEAMERAGTEWKLSTPRGRVKARQVLVATGGNS
PYPPFGWFRRRIVTVGSFVIATDPLPADLAAHLFPTRRNYVTSRIIGNYFRLTADNRLIFGGRARFALSNPASDAKSGRI
LEAKLRELFPELAGVGIAACWGGAIDLTADRLPRAGEHDGLFYAMGYSGHGVQMATAMGEHMARVMGGDASANPFADLTW
PAIPGHLGKPWFLPMVGAWYRLQDWLH
>Mature_426_residues
TPQSLSYWQASTPPFASGEKARVEGDCDVAIIGGGFTGLSAALELARGGASVVLLEAGRIAGAASGRNGGQCNAGTAHDF
GALASAHGVDLARKWYLAHVDAVETVGRIAAREGIACDYTRCGRAKLAAKPEHFAKLEAAHAVLLKEVDPNVLLVGPGEL
SSEINARGFHGALVQTTSAQLHPGKLGVGLGEAAVRAGARIWENAPVEAMERAGTEWKLSTPRGRVKARQVLVATGGNSP
YPPFGWFRRRIVTVGSFVIATDPLPADLAAHLFPTRRNYVTSRIIGNYFRLTADNRLIFGGRARFALSNPASDAKSGRIL
EAKLRELFPELAGVGIAACWGGAIDLTADRLPRAGEHDGLFYAMGYSGHGVQMATAMGEHMARVMGGDASANPFADLTWP
AIPGHLGKPWFLPMVGAWYRLQDWLH

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=433, Percent_Identity=33.7182448036951, Blast_Score=206, Evalue=3e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 45232; Mature: 45101

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPQSLSYWQASTPPFASGEKARVEGDCDVAIIGGGFTGLSAALELARGGASVVLLEAGR
CCCCCCCCCCCCCCCCCCCCCEEECCCCCEEEECCCHHHHHHHHHHHCCCCEEEEEECCC
IAGAASGRNGGQCNAGTAHDFGALASAHGVDLARKWYLAHVDAVETVGRIAAREGIACDY
EEECCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEECC
TRCGRAKLAAKPEHFAKLEAAHAVLLKEVDPNVLLVGPGELSSEINARGFHGALVQTTSA
HHCCCCCCCCCCHHHHHHHHHHHHHEEECCCCEEEECCCCCCCCCCCCCCCCEEEEECCC
QLHPGKLGVGLGEAAVRAGARIWENAPVEAMERAGTEWKLSTPRGRVKARQVLVATGGNS
CCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCEEECCCCCCCEEEEEEEEECCCC
PYPPFGWFRRRIVTVGSFVIATDPLPADLAAHLFPTRRNYVTSRIIGNYFRLTADNRLIF
CCCCHHHHHHHHHHHCCEEEECCCCCHHHHHHHCCCHHHHHHHHHHCCEEEEEECCEEEE
GGRARFALSNPASDAKSGRILEAKLRELFPELAGVGIAACWGGAIDLTADRLPRAGEHDG
CCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCEEEEEHHHCCCCCCCCC
LFYAMGYSGHGVQMATAMGEHMARVMGGDASANPFADLTWPAIPGHLGKPWFLPMVGAWY
EEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
RLQDWLH
HHHHHCC
>Mature Secondary Structure 
TPQSLSYWQASTPPFASGEKARVEGDCDVAIIGGGFTGLSAALELARGGASVVLLEAGR
CCCCCCCCCCCCCCCCCCCCEEECCCCCEEEECCCHHHHHHHHHHHCCCCEEEEEECCC
IAGAASGRNGGQCNAGTAHDFGALASAHGVDLARKWYLAHVDAVETVGRIAAREGIACDY
EEECCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEECC
TRCGRAKLAAKPEHFAKLEAAHAVLLKEVDPNVLLVGPGELSSEINARGFHGALVQTTSA
HHCCCCCCCCCCHHHHHHHHHHHHHEEECCCCEEEECCCCCCCCCCCCCCCCEEEEECCC
QLHPGKLGVGLGEAAVRAGARIWENAPVEAMERAGTEWKLSTPRGRVKARQVLVATGGNS
CCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCEEECCCCCCCEEEEEEEEECCCC
PYPPFGWFRRRIVTVGSFVIATDPLPADLAAHLFPTRRNYVTSRIIGNYFRLTADNRLIF
CCCCHHHHHHHHHHHCCEEEECCCCCHHHHHHHCCCHHHHHHHHHHCCEEEEEECCEEEE
GGRARFALSNPASDAKSGRILEAKLRELFPELAGVGIAACWGGAIDLTADRLPRAGEHDG
CCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCEEEEEHHHCCCCCCCCC
LFYAMGYSGHGVQMATAMGEHMARVMGGDASANPFADLTWPAIPGHLGKPWFLPMVGAWY
EEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
RLQDWLH
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]