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The map label for this gene is manX [C]

Identifier: 87200915

GI number: 87200915

Start: 3120524

End: 3120928

Strand: Reverse

Name: manX [C]

Synonym: Saro_2903

Alternate gene names: 87200915

Gene position: 3120928-3120524 (Counterclockwise)

Preceding gene: 87200916

Following gene: 87200914

Centisome position: 87.63

GC content: 60.49

Gene sequence:

>405_bases
ATGATCGGTCTGATTCTCGTTACCCACGGCGCCCTTGCCGATGAATTCATCCATGCGATGGAGCATGTGGTTGGCCGGCA
AAGCGATGTCATCGGCGTCTGCATCGGCCCCAACGACGACATGGAGAAGCGCCGCAAGGAGATTGCCGACGCGATCAAGC
GCGTGGACAGCGGCGAAGGCGTTATCATCCTGACCGACCTGTTCGGCGGAACGCCCTCCAACCTCGCGATCTCGCTCATG
CAGGCAGGCAAGATCGAAGTCATTGCCGGTATCAACCTGCCCATGCTGATCCGCCTGGCCAAGGCGCGCAACTGCATGGG
CGTCAAGGAAGCGACTGCGGCGGCGCGCGACGCCGGACGCAACTACATAACGATCGCTTCCGAATTTCTCGGACAGGATG
CATGA

Upstream 100 bases:

>100_bases
TCGATACCCAGTCGTCTTGCGTCCCCCGTATTCACGCCAAGCCTGCGTTAAGCCGCGCTTCGGCAGGATACACGGTCGCA
CCTCGGAGCCTGTCCCTTTC

Downstream 100 bases:

>100_bases
TATGGCGGAACAGGGCGCGCAGCAGGGATACGTCACTCGCGAAACGGTGACGATCGTCAACCAGCGCGGCCTTCATGCCC
GGGCCAGCGCGAAGTTCGTG

Product: PTS system fructose subfamily IIA component

Products: protein histidine; sugar phosphate

Alternate protein names: PTS System IIA Component; PTS System Mannose/Fructose/Sorbose Family IIA Subunit; PTS System Fructose IIA Component; PTS System Mannose-Specific IIAB Component; PTS System IIA Component Man Family; PTS System Protein; IIA Component Of Sugar Transport PTS System; Pts System Fructose Subfamily IIa Component; Fructose-Specific IIA Component; PTS System Transporter Subunit IIA; Protein-N P-Phosphohistidine-Sugar Phosphotransferase; PTS System Mannose/Fructose-Specific IIA Component; PTS System IIa Component; PTS Mannose-Specific IIA Component; Pts System Mannose-Specific Eiiab Component; PTS Mannose-Specific IIA Component; PTS System Mannose Subfamily IIA Subunit; PTS System Mannose/Fructose/Sorbose Family IIB Component; Mannose-Specific Phosphotransferase System Component IIAB; Pts System Mannose/Fructose/Sorbose Family IIa Component; Phosphotransferase System Subunit; PTS System Fructose Subfamily Transprotein Subunit IIA; AhaA Protein; Pts System Fructose IIA Component; PTS System Mannose/Fructose-Specific Component IIA; Mannose-Specific PTS System Component IIAB; Phosphotransferase System Fructose Subfamily IIA Component; PTS System Mannose/Fructose/Sorbose Family IIAB Subunit; Mannose-Specific Phosphotransferase System Component IIA; PTS System Mannose-Specific II AB Component; PTS System Mannose-Type Transporter Subunit IIA; Pentitol Phosphotransferase IIA Component; PTS System Component IIA; PTS System Mannose-Specific Transporter Subunit IIAB; Phosphotransferase System IIA Component; PTS System Mannose/Fructose IIA Component; Phosphotransferase Mannose/Fructose-Specific Component IIA; PTS System Mannose/Fructose/Sorbose Family IIA Component; PTS System Mannose-Specific IIAB Components; PTS System Mannose-Specific Transporter Subunit IIA; PTS System Sorbose Subfamily IIB Component; Phosphotransferase System Fructose-Specific IIA

Number of amino acids: Translated: 134; Mature: 134

Protein sequence:

>134_residues
MIGLILVTHGALADEFIHAMEHVVGRQSDVIGVCIGPNDDMEKRRKEIADAIKRVDSGEGVIILTDLFGGTPSNLAISLM
QAGKIEVIAGINLPMLIRLAKARNCMGVKEATAAARDAGRNYITIASEFLGQDA

Sequences:

>Translated_134_residues
MIGLILVTHGALADEFIHAMEHVVGRQSDVIGVCIGPNDDMEKRRKEIADAIKRVDSGEGVIILTDLFGGTPSNLAISLM
QAGKIEVIAGINLPMLIRLAKARNCMGVKEATAAARDAGRNYITIASEFLGQDA
>Mature_134_residues
MIGLILVTHGALADEFIHAMEHVVGRQSDVIGVCIGPNDDMEKRRKEIADAIKRVDSGEGVIILTDLFGGTPSNLAISLM
QAGKIEVIAGINLPMLIRLAKARNCMGVKEATAAARDAGRNYITIASEFLGQDA

Specific function: This Is A Component Of The Phosphoenolpyruvate-Dependent Sugar Phosphotransferase System (Pts), A Major Carbohydrate Active -Transport System. The IIcd Domains Contain The Sugar Binding Site And The Transmembrane Channel; The IIa Domain Contains The Prim

COG id: COG2893

COG function: function code G; Phosphotransferase system, mannose/fructose-specific component IIA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788120, Length=121, Percent_Identity=30.5785123966942, Blast_Score=62, Evalue=1e-11,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.1.69

Molecular weight: Translated: 14257; Mature: 14257

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS51096 PTS_EIIA_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGLILVTHGALADEFIHAMEHVVGRQSDVIGVCIGPNDDMEKRRKEIADAIKRVDSGEG
CEEEEEEECCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCC
VIILTDLFGGTPSNLAISLMQAGKIEVIAGINLPMLIRLAKARNCMGVKEATAAARDAGR
EEEEEECCCCCCCHHHHHEECCCCEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHCCC
NYITIASEFLGQDA
CEEEEEHHHCCCCC
>Mature Secondary Structure
MIGLILVTHGALADEFIHAMEHVVGRQSDVIGVCIGPNDDMEKRRKEIADAIKRVDSGEG
CEEEEEEECCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCC
VIILTDLFGGTPSNLAISLMQAGKIEVIAGINLPMLIRLAKARNCMGVKEATAAARDAGR
EEEEEECCCCCCCHHHHHEECCCCEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHCCC
NYITIASEFLGQDA
CEEEEEHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA