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The map label for this gene is truA

Identifier: 87200906

GI number: 87200906

Start: 3113088

End: 3113852

Strand: Reverse

Name: truA

Synonym: Saro_2893

Alternate gene names: 87200906

Gene position: 3113852-3113088 (Counterclockwise)

Preceding gene: 87200907

Following gene: 87200905

Centisome position: 87.43

GC content: 66.54

Gene sequence:

>765_bases
TTGGTTGACGTCCCTACCCGCTTCGCGCTGACCCTCGAATGGAACGGCGGTCCCTACATGGGCTTCCAGCGCCAGTCGCA
CGGCCCCAGCGTCCAGCAGGCGGTGGAGGAAGCGGTCCATTCCGTCACCGGCGAGGCGGTCGCGCTCAATGCGGCGGGCC
GCACCGATACCGGGGTCCATGCGCTTGGCATGCGCGCGCATTTCGACCTGTCGCGCGCCTTCGATCCGTTCCGCATGATG
GAGGCGCTGAATGCGCGCCTGCGGCTTGCCGGGCACCCGGTTGCGGTGCTGGATTGCATCGAGGTGGCGCCGGACTGGCA
CGCCCGCTTCTCCTGCACCGGTCGTGCCTATGAATATCGCATCATCAACCGCCGCGCCCCGCTGACGCTCGATCTCGGTC
GCGCGTGGCGCATCGCCAAGCCCCTTGACGAGACGGTAATGCACGAGGCGGCGCAACTGCTTGTCGGCCATCATGATTTC
ACCACCTTCCGTTCGGTCCATTGCCAGTCGGCCAGCCCGTTGAAGACGCTCGACCGGCTCGACGTGCGGCGCGACGGCGA
CCGGGTCATCGTCGAGGCCGCAGCGCGCAGCTTTCTCCACCACCAGGTCCGCTCGATGGTGGGATGCCTTGCACTGGTCG
GGCAGGGGCAATGGAGCGTCGCCCAGCTGGGCGAGGCGCTGGCCGCAAAAGACCGTAGCAGATTAGGACTTAACGCGCCC
CCGGAAGGCCTGTACTTCGTCTCCGCTCAATATCCCGGAGAATGA

Upstream 100 bases:

>100_bases
ACCGGCGCGATCCGACCGACCCTGATCCAGCGCGCGGGTCGCCCGGCCATGGACACTGCCTCGCTGCTGCGCGGGCGCGC
CATTCCCGGGGGAACGCGTC

Downstream 100 bases:

>100_bases
TCCCATGACCCAGCTTCGCGGCCAGCGCATCGTGTCCCAGCTCGATGCCGACGGCACCCTCGCCGTCTCGCTCCAGGACT
TCGACGTGCCCGAACCCACC

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MVDVPTRFALTLEWNGGPYMGFQRQSHGPSVQQAVEEAVHSVTGEAVALNAAGRTDTGVHALGMRAHFDLSRAFDPFRMM
EALNARLRLAGHPVAVLDCIEVAPDWHARFSCTGRAYEYRIINRRAPLTLDLGRAWRIAKPLDETVMHEAAQLLVGHHDF
TTFRSVHCQSASPLKTLDRLDVRRDGDRVIVEAAARSFLHHQVRSMVGCLALVGQGQWSVAQLGEALAAKDRSRLGLNAP
PEGLYFVSAQYPGE

Sequences:

>Translated_254_residues
MVDVPTRFALTLEWNGGPYMGFQRQSHGPSVQQAVEEAVHSVTGEAVALNAAGRTDTGVHALGMRAHFDLSRAFDPFRMM
EALNARLRLAGHPVAVLDCIEVAPDWHARFSCTGRAYEYRIINRRAPLTLDLGRAWRIAKPLDETVMHEAAQLLVGHHDF
TTFRSVHCQSASPLKTLDRLDVRRDGDRVIVEAAARSFLHHQVRSMVGCLALVGQGQWSVAQLGEALAAKDRSRLGLNAP
PEGLYFVSAQYPGE
>Mature_254_residues
MVDVPTRFALTLEWNGGPYMGFQRQSHGPSVQQAVEEAVHSVTGEAVALNAAGRTDTGVHALGMRAHFDLSRAFDPFRMM
EALNARLRLAGHPVAVLDCIEVAPDWHARFSCTGRAYEYRIINRRAPLTLDLGRAWRIAKPLDETVMHEAAQLLVGHHDF
TTFRSVHCQSASPLKTLDRLDVRRDGDRVIVEAAARSFLHHQVRSMVGCLALVGQGQWSVAQLGEALAAKDRSRLGLNAP
PEGLYFVSAQYPGE

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=250, Percent_Identity=36.4, Blast_Score=109, Evalue=2e-24,
Organism=Escherichia coli, GI1788657, Length=247, Percent_Identity=40.4858299595142, Blast_Score=170, Evalue=7e-44,
Organism=Drosophila melanogaster, GI116007752, Length=300, Percent_Identity=26.3333333333333, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 28015; Mature: 28015

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDVPTRFALTLEWNGGPYMGFQRQSHGPSVQQAVEEAVHSVTGEAVALNAAGRTDTGVH
CCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCE
ALGMRAHFDLSRAFDPFRMMEALNARLRLAGHPVAVLDCIEVAPDWHARFSCTGRAYEYR
EECCEEECCHHHCCCHHHHHHHHCHHEEECCCCCHHHHHHHHCCCCCCEEEECCCEEEEE
IINRRAPLTLDLGRAWRIAKPLDETVMHEAAQLLVGHHDFTTFRSVHCQSASPLKTLDRL
EECCCCCEEEECCCCEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHH
DVRRDGDRVIVEAAARSFLHHQVRSMVGCLALVGQGQWSVAQLGEALAAKDRSRLGLNAP
HCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHCCCCCCC
PEGLYFVSAQYPGE
CCCEEEEEECCCCC
>Mature Secondary Structure
MVDVPTRFALTLEWNGGPYMGFQRQSHGPSVQQAVEEAVHSVTGEAVALNAAGRTDTGVH
CCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCE
ALGMRAHFDLSRAFDPFRMMEALNARLRLAGHPVAVLDCIEVAPDWHARFSCTGRAYEYR
EECCEEECCHHHCCCHHHHHHHHCHHEEECCCCCHHHHHHHHCCCCCCEEEECCCEEEEE
IINRRAPLTLDLGRAWRIAKPLDETVMHEAAQLLVGHHDFTTFRSVHCQSASPLKTLDRL
EECCCCCEEEECCCCEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHH
DVRRDGDRVIVEAAARSFLHHQVRSMVGCLALVGQGQWSVAQLGEALAAKDRSRLGLNAP
HCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHCCCCCCC
PEGLYFVSAQYPGE
CCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA