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The map label for this gene is fmt

Identifier: 87200907

GI number: 87200907

Start: 3113845

End: 3114750

Strand: Reverse

Name: fmt

Synonym: Saro_2894

Alternate gene names: 87200907

Gene position: 3114750-3113845 (Counterclockwise)

Preceding gene: 87200912

Following gene: 87200906

Centisome position: 87.45

GC content: 69.87

Gene sequence:

>906_bases
ATGCGCATCATCTTCATGGGGACGCCCGATTTCGCGGTGCCGACGCTGGAGGCGCTCGTCGCGGCCGGGCACGATGTGGT
CGCCGCCTACAGCCAGCCGCCGCGTCCGGCGGGACGGGGCAAGAAGCTGCAACCCTCGCCCGTCCATCTGGCCGCCGAAG
CCCACGGGATCGACGTGCGCACGCCCGTCTCTCTCAAAGGGGCGGATGAGCAGACCACGCTTGCCGCGTTCGATGCGGAT
GTGGCGGTCGTCGCGGCCTATGGCCTGATCCTGCCGCAGGCCGTGCTGGATGCGCCGCGCCTCGGCTGCCTCAACGTCCA
CGGCTCGCTCCTGCCGCGTTGGCGCGGCGCCGCGCCGGTCCAGCGCGCGATCCTCGCGGGCGACGAGATGACCGGCGTCA
CCATCATGCAGATGGAGCGGGGACTCGATACCGGGCCGATGCTGGCGAGGATCGAAACTCCGGTCGACGGCAAGACCGCG
GGCGATCTCACCGCCGAACTTGCGGTGAAAGGTGCCGCGCTGATGGTGCAGGTGCTGGCCGACCTGGCGAGCTATCCTGC
GGTGGTGCAGCCGGAGCAGGGCGTGACCTACGCCCACAAGATCGACAAGGCGGAGAGCCGGTTGGACTTCACCCGCGATG
CGGTGGACGTGGAACGGCAAGTCCGCGCCTTTTCCCCGGCTCCCGGTGCCTTCTTCGAACTTGAAGGCGAACGCTACCGC
GTGCTGGCGGCCGAAGTCCTCGGCGTGGCGGGCGAACCGGGCGTAACGGTCGACGACGTGCTTGCCATTGCCTGCGGCAC
CGGCGCGATCCGACCGACCCTGATCCAGCGCGCGGGTCGCCCGGCCATGGACACTGCCTCGCTGCTGCGCGGGCGCGCCA
TTCCCGGGGGAACGCGTCTTGGTTGA

Upstream 100 bases:

>100_bases
GGAGTCGCGCCAGAGCGCCGGAAAGTGCCTCGATCTCTTGCGATGCCATGGCAGCACAGATAGGGCCGGAGCCGACAAAC
GGAAGGCCTTCTGACAGCTT

Downstream 100 bases:

>100_bases
CGTCCCTACCCGCTTCGCGCTGACCCTCGAATGGAACGGCGGTCCCTACATGGGCTTCCAGCGCCAGTCGCACGGCCCCA
GCGTCCAGCAGGCGGTGGAG

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MRIIFMGTPDFAVPTLEALVAAGHDVVAAYSQPPRPAGRGKKLQPSPVHLAAEAHGIDVRTPVSLKGADEQTTLAAFDAD
VAVVAAYGLILPQAVLDAPRLGCLNVHGSLLPRWRGAAPVQRAILAGDEMTGVTIMQMERGLDTGPMLARIETPVDGKTA
GDLTAELAVKGAALMVQVLADLASYPAVVQPEQGVTYAHKIDKAESRLDFTRDAVDVERQVRAFSPAPGAFFELEGERYR
VLAAEVLGVAGEPGVTVDDVLAIACGTGAIRPTLIQRAGRPAMDTASLLRGRAIPGGTRLG

Sequences:

>Translated_301_residues
MRIIFMGTPDFAVPTLEALVAAGHDVVAAYSQPPRPAGRGKKLQPSPVHLAAEAHGIDVRTPVSLKGADEQTTLAAFDAD
VAVVAAYGLILPQAVLDAPRLGCLNVHGSLLPRWRGAAPVQRAILAGDEMTGVTIMQMERGLDTGPMLARIETPVDGKTA
GDLTAELAVKGAALMVQVLADLASYPAVVQPEQGVTYAHKIDKAESRLDFTRDAVDVERQVRAFSPAPGAFFELEGERYR
VLAAEVLGVAGEPGVTVDDVLAIACGTGAIRPTLIQRAGRPAMDTASLLRGRAIPGGTRLG
>Mature_301_residues
MRIIFMGTPDFAVPTLEALVAAGHDVVAAYSQPPRPAGRGKKLQPSPVHLAAEAHGIDVRTPVSLKGADEQTTLAAFDAD
VAVVAAYGLILPQAVLDAPRLGCLNVHGSLLPRWRGAAPVQRAILAGDEMTGVTIMQMERGLDTGPMLARIETPVDGKTA
GDLTAELAVKGAALMVQVLADLASYPAVVQPEQGVTYAHKIDKAESRLDFTRDAVDVERQVRAFSPAPGAFFELEGERYR
VLAAEVLGVAGEPGVTVDDVLAIACGTGAIRPTLIQRAGRPAMDTASLLRGRAIPGGTRLG

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI164663775, Length=221, Percent_Identity=35.7466063348416, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI238814322, Length=294, Percent_Identity=26.530612244898, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI21614513, Length=288, Percent_Identity=27.7777777777778, Blast_Score=88, Evalue=8e-18,
Organism=Escherichia coli, GI1789683, Length=301, Percent_Identity=49.1694352159468, Blast_Score=275, Evalue=3e-75,
Organism=Escherichia coli, GI1788589, Length=308, Percent_Identity=27.2727272727273, Blast_Score=91, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI133930964, Length=264, Percent_Identity=26.5151515151515, Blast_Score=81, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6319458, Length=263, Percent_Identity=28.5171102661597, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI45550868, Length=228, Percent_Identity=30.7017543859649, Blast_Score=97, Evalue=9e-21,
Organism=Drosophila melanogaster, GI28571984, Length=150, Percent_Identity=35.3333333333333, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24585660, Length=243, Percent_Identity=28.3950617283951, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_NOVAD (Q2G493)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_498164.1
- ProteinModelPortal:   Q2G493
- SMR:   Q2G493
- STRING:   Q2G493
- GeneID:   3915533
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_2894
- NMPDR:   fig|48935.1.peg.3546
- eggNOG:   COG0223
- HOGENOM:   HBG571560
- OMA:   IMQMDEG
- PhylomeDB:   Q2G493
- ProtClustDB:   CLSK844891
- BioCyc:   NARO279238:SARO_2894-MONOMER
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 31476; Mature: 31476

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIIFMGTPDFAVPTLEALVAAGHDVVAAYSQPPRPAGRGKKLQPSPVHLAAEAHGIDVR
CEEEEECCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCCC
TPVSLKGADEQTTLAAFDADVAVVAAYGLILPQAVLDAPRLGCLNVHGSLLPRWRGAAPV
CCCEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCHH
QRAILAGDEMTGVTIMQMERGLDTGPMLARIETPVDGKTAGDLTAELAVKGAALMVQVLA
HHHHHCCCCCCCEEEEEHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHH
DLASYPAVVQPEQGVTYAHKIDKAESRLDFTRDAVDVERQVRAFSPAPGAFFELEGERYR
HHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEE
VLAAEVLGVAGEPGVTVDDVLAIACGTGAIRPTLIQRAGRPAMDTASLLRGRAIPGGTRL
EEEHHHHCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHCCCCCHHHHHHHHCCCCCCCCCC
G
C
>Mature Secondary Structure
MRIIFMGTPDFAVPTLEALVAAGHDVVAAYSQPPRPAGRGKKLQPSPVHLAAEAHGIDVR
CEEEEECCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCCC
TPVSLKGADEQTTLAAFDADVAVVAAYGLILPQAVLDAPRLGCLNVHGSLLPRWRGAAPV
CCCEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCHH
QRAILAGDEMTGVTIMQMERGLDTGPMLARIETPVDGKTAGDLTAELAVKGAALMVQVLA
HHHHHCCCCCCCEEEEEHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHH
DLASYPAVVQPEQGVTYAHKIDKAESRLDFTRDAVDVERQVRAFSPAPGAFFELEGERYR
HHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEE
VLAAEVLGVAGEPGVTVDDVLAIACGTGAIRPTLIQRAGRPAMDTASLLRGRAIPGGTRL
EEEHHHHCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHCCCCCHHHHHHHHCCCCCCCCCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA