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The map label for this gene is ligA [H]

Identifier: 87200826

GI number: 87200826

Start: 3037312

End: 3037749

Strand: Reverse

Name: ligA [H]

Synonym: Saro_2813

Alternate gene names: 87200826

Gene position: 3037749-3037312 (Counterclockwise)

Preceding gene: 87200827

Following gene: 87200825

Centisome position: 85.29

GC content: 61.42

Gene sequence:

>438_bases
GTGACTGACAACAGCTCGACCGATAAGCCCAAGCCTGTGCAGAACATCCACGAGTACCTCGCCGAGTTCGAGGACATCCC
CGGCACGCGCATCTATACCGCTGCCCGCGCCCGCAAGGGGTACTGGATCAACCAGTTCGCGATGAGCCTGATGAAGCCGG
AGAACCGCGAGCGGTTCAAGGCGAACGAGCGGGCCTATCTTGACGAATGGAAGATCAGCGAGGAGGCCAAGGAAGCGCTG
CTCGCGCGGGACTACAACCGCCTGCTCGACCTTGGCGGCAACGTCTATTTCCTGTCGAAGCTGTTCTCCTCGGACGGACT
GCCGTTCGCCGAGGCGGTCAGCACGATGACCGACATGACCTGGCCGGAATACCGCCAGATGATGCTGGACGGCGGACGCA
AGCCCGAAGGCAACCGATCGATCAAGGGAGGCTATTGA

Upstream 100 bases:

>100_bases
ACGCGCTCGACATCAGCGATGCTGAACGCCACGCGATCTTCGAGGGCAACGCGCGCCGCGTGTTCCCGCGCCTCGACGCC
AAGCTGAAGGAGAGGGGCCT

Downstream 100 bases:

>100_bases
TATGGCCCGCATAACCGCTGGCGTTGGCTCCAGCCACGTTCCGCTGCTGGGTGTCGCTGTTGATCAGGGCAAGTGGCAGG
ACGACTATTTCGGCCCGATC

Product: protocatechuate 4,5-dioxygenase subunit alpha

Products: NA

Alternate protein names: 4,5-PCD [H]

Number of amino acids: Translated: 145; Mature: 144

Protein sequence:

>145_residues
MTDNSSTDKPKPVQNIHEYLAEFEDIPGTRIYTAARARKGYWINQFAMSLMKPENRERFKANERAYLDEWKISEEAKEAL
LARDYNRLLDLGGNVYFLSKLFSSDGLPFAEAVSTMTDMTWPEYRQMMLDGGRKPEGNRSIKGGY

Sequences:

>Translated_145_residues
MTDNSSTDKPKPVQNIHEYLAEFEDIPGTRIYTAARARKGYWINQFAMSLMKPENRERFKANERAYLDEWKISEEAKEAL
LARDYNRLLDLGGNVYFLSKLFSSDGLPFAEAVSTMTDMTWPEYRQMMLDGGRKPEGNRSIKGGY
>Mature_144_residues
TDNSSTDKPKPVQNIHEYLAEFEDIPGTRIYTAARARKGYWINQFAMSLMKPENRERFKANERAYLDEWKISEEAKEALL
ARDYNRLLDLGGNVYFLSKLFSSDGLPFAEAVSTMTDMTWPEYRQMMLDGGRKPEGNRSIKGGY

Specific function: Responsible for the aromatic ring fission of protocatechuate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014159
- InterPro:   IPR011986 [H]

Pfam domain/function: PF07746 LigA [H]

EC number: =1.13.11.8 [H]

Molecular weight: Translated: 16761; Mature: 16630

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDNSSTDKPKPVQNIHEYLAEFEDIPGTRIYTAARARKGYWINQFAMSLMKPENRERFK
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEHHHHHHHCCCHHHHHHHHHHCCCCHHHHH
ANERAYLDEWKISEEAKEALLARDYNRLLDLGGNVYFLSKLFSSDGLPFAEAVSTMTDMT
HCCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHCC
WPEYRQMMLDGGRKPEGNRSIKGGY
CHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TDNSSTDKPKPVQNIHEYLAEFEDIPGTRIYTAARARKGYWINQFAMSLMKPENRERFK
CCCCCCCCCCHHHHHHHHHHHHHCCCCCEEHHHHHHHCCCHHHHHHHHHHCCCCHHHHH
ANERAYLDEWKISEEAKEALLARDYNRLLDLGGNVYFLSKLFSSDGLPFAEAVSTMTDMT
HCCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHCC
WPEYRQMMLDGGRKPEGNRSIKGGY
CHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2185230 [H]