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The map label for this gene is ligB [H]

Identifier: 87200825

GI number: 87200825

Start: 3036468

End: 3037310

Strand: Reverse

Name: ligB [H]

Synonym: Saro_2812

Alternate gene names: 87200825

Gene position: 3037310-3036468 (Counterclockwise)

Preceding gene: 87200826

Following gene: 87200824

Centisome position: 85.28

GC content: 63.35

Gene sequence:

>843_bases
ATGGCCCGCATAACCGCTGGCGTTGGCTCCAGCCACGTTCCGCTGCTGGGTGTCGCTGTTGATCAGGGCAAGTGGCAGGA
CGACTATTTCGGCCCGATCTTCAAGGGTTATGAATGGACCCGCGAATGGGAAAAGCGCGAGAAGCCCGATGTGGTCATTC
TGGTCTACAACGACCACGCTTCGGCATTCGACGCGAACATCATCCCGACCTTCGCAATCGGCTGCGGCGAGCACTACAAG
TCGGCCGATGAAGGCTGGGGCCCGCGCCCGGTGCCCGACGTGGAAGGCCATGCCGACCTTGCCTGGCACATCGCGCAGAG
CCTGATCCTCGACGATTTCGACATGACCATCATCAACGAGATGGATGTGGACCACGGCCTGACCGTGCCGCTCTCGATGA
TGTTCGGCCAGCCCGAGAAGTGGCCGTGCAAGGTCGTGCCGCTGGCGGTGAACGTCGTCACCTATCCGGTGCCGTCGGGC
AACCGCTGCTGGGCGCTGGGCGAGGCGATCGCCCGCGCGGTGGAAAGCTTCCCCGAGGACCTCAACGTGCAGATCTGGGG
CACGGGCGGCATGAGCCACCAGCTCCAGGGCCCGCGCGCCGGCCTGCTCAACCGCGAGTGGGACAACAAGTTCCTCGACA
TGCTGGAATCGGACAACGACGATGTCCGCTACATTCCGCATATCGAATACCTGCGCGAGACCGGCTCGGAAGGCATCGAG
ATGGTCATGTGGCTGATCATGCGCGGCGCGCTCGGCAAGAAGGTCAAGCGCCTGAACCGCCATTACCACATTCCCTGCAG
CAACACCGCGATCGGGCACATCGTGCTCGAGCCCGCGGACTGA

Upstream 100 bases:

>100_bases
AGCACGATGACCGACATGACCTGGCCGGAATACCGCCAGATGATGCTGGACGGCGGACGCAAGCCCGAAGGCAACCGATC
GATCAAGGGAGGCTATTGAT

Downstream 100 bases:

>100_bases
CTGACCAGACAGGAGTAGTACCCATGCGTATCGCACTGGCCGGGGCGGGAGCCTTCGGCGAAAAGCACCTCGATGGCCTG
AAGAACATCGATGGCGTCGA

Product: protocatechuate 4,5-dioxygenase subunit beta

Products: NA

Alternate protein names: 4,5-PCD [H]

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MARITAGVGSSHVPLLGVAVDQGKWQDDYFGPIFKGYEWTREWEKREKPDVVILVYNDHASAFDANIIPTFAIGCGEHYK
SADEGWGPRPVPDVEGHADLAWHIAQSLILDDFDMTIINEMDVDHGLTVPLSMMFGQPEKWPCKVVPLAVNVVTYPVPSG
NRCWALGEAIARAVESFPEDLNVQIWGTGGMSHQLQGPRAGLLNREWDNKFLDMLESDNDDVRYIPHIEYLRETGSEGIE
MVMWLIMRGALGKKVKRLNRHYHIPCSNTAIGHIVLEPAD

Sequences:

>Translated_280_residues
MARITAGVGSSHVPLLGVAVDQGKWQDDYFGPIFKGYEWTREWEKREKPDVVILVYNDHASAFDANIIPTFAIGCGEHYK
SADEGWGPRPVPDVEGHADLAWHIAQSLILDDFDMTIINEMDVDHGLTVPLSMMFGQPEKWPCKVVPLAVNVVTYPVPSG
NRCWALGEAIARAVESFPEDLNVQIWGTGGMSHQLQGPRAGLLNREWDNKFLDMLESDNDDVRYIPHIEYLRETGSEGIE
MVMWLIMRGALGKKVKRLNRHYHIPCSNTAIGHIVLEPAD
>Mature_279_residues
ARITAGVGSSHVPLLGVAVDQGKWQDDYFGPIFKGYEWTREWEKREKPDVVILVYNDHASAFDANIIPTFAIGCGEHYKS
ADEGWGPRPVPDVEGHADLAWHIAQSLILDDFDMTIINEMDVDHGLTVPLSMMFGQPEKWPCKVVPLAVNVVTYPVPSGN
RCWALGEAIARAVESFPEDLNVQIWGTGGMSHQLQGPRAGLLNREWDNKFLDMLESDNDDVRYIPHIEYLRETGSEGIEM
VMWLIMRGALGKKVKRLNRHYHIPCSNTAIGHIVLEPAD

Specific function: Responsible for the aromatic ring fission of protocatechuate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786544, Length=187, Percent_Identity=32.0855614973262, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004183 [H]

Pfam domain/function: PF02900 LigB [H]

EC number: =1.13.11.8 [H]

Molecular weight: Translated: 31416; Mature: 31285

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARITAGVGSSHVPLLGVAVDQGKWQDDYFGPIFKGYEWTREWEKREKPDVVILVYNDHA
CCEEECCCCCCCCCEEEEEECCCCCCCCCCCHHHCCCHHHHHHHHCCCCCEEEEEECCCC
SAFDANIIPTFAIGCGEHYKSADEGWGPRPVPDVEGHADLAWHIAQSLILDDFDMTIINE
CCCCCCCCEEEEECCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEC
MDVDHGLTVPLSMMFGQPEKWPCKVVPLAVNVVTYPVPSGNRCWALGEAIARAVESFPED
CCCCCCCCCCHHHHCCCCCCCCEEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCC
LNVQIWGTGGMSHQLQGPRAGLLNREWDNKFLDMLESDNDDVRYIPHIEYLRETGSEGIE
CCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEECCHHHHHHHCCCHHHH
MVMWLIMRGALGKKVKRLNRHYHIPCSNTAIGHIVLEPAD
HHHHHHHHHHHHHHHHHHCCEEECCCCCCCEEEEEEECCC
>Mature Secondary Structure 
ARITAGVGSSHVPLLGVAVDQGKWQDDYFGPIFKGYEWTREWEKREKPDVVILVYNDHA
CEEECCCCCCCCCEEEEEECCCCCCCCCCCHHHCCCHHHHHHHHCCCCCEEEEEECCCC
SAFDANIIPTFAIGCGEHYKSADEGWGPRPVPDVEGHADLAWHIAQSLILDDFDMTIINE
CCCCCCCCEEEEECCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEC
MDVDHGLTVPLSMMFGQPEKWPCKVVPLAVNVVTYPVPSGNRCWALGEAIARAVESFPED
CCCCCCCCCCHHHHCCCCCCCCEEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCC
LNVQIWGTGGMSHQLQGPRAGLLNREWDNKFLDMLESDNDDVRYIPHIEYLRETGSEGIE
CCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEECCHHHHHHHCCCHHHH
MVMWLIMRGALGKKVKRLNRHYHIPCSNTAIGHIVLEPAD
HHHHHHHHHHHHHHHHHHCCEEECCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2185230 [H]