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The map label for this gene is nifS1 [H]

Identifier: 87200811

GI number: 87200811

Start: 3021704

End: 3022750

Strand: Reverse

Name: nifS1 [H]

Synonym: Saro_2798

Alternate gene names: 87200811

Gene position: 3022750-3021704 (Counterclockwise)

Preceding gene: 87200813

Following gene: 87200810

Centisome position: 84.87

GC content: 67.81

Gene sequence:

>1047_bases
TTGCCATCCAGACCGATCTATCTCGACCATGCCGCCACGACGCCCCTGCTGCCCGAGGCAATGGCTGCCATGGAAGCGGG
GTGGCGCGTCTGGGCCAACCCTTCGAGCCCGCACGCCGCAGGCCGGGCCGCGCGTGCTGCGCTGGAGGATGCGCGGGCGC
GGTTGAAGGCAGCGCTCGGCTGGCGGGGCGAAGTGATCTTCACCTCGGGCGCAAGCGAGGCCCTGGCCATCGGCATTGGC
CGCGCCAAGGTGGGGCAAGTTCTTGTTTCCGCTGTTGAACACGACGCGGCGCTGCGGGTGGCGGGCACCGCGCCGAGGCT
GCCCGTGGGGGCCGACGGCCGGGTCGACCCGAACGGCACCGAACGATACATGAGCGGCACCGACCGCGCGCTTTACGTGG
TGCAGCAGGTCAATAGCGAGACCGGCGTGATACAGGACATCGCGGCGCTGGCAGACCGGGTCCGCAGCGGGGGCCATCTC
CTGCTGTGCGACGCCGCGCAGGGGGCCGGAAAGATCGATTTGCCCGATGCGGACATGGTTGTCCTCTCCGCCCACAAGTT
CGGAGGGCCTGTGGGAGTCGGCGCGCTGCTGGTGCGCGACTACGGGCTGCTGGAGCCGAGCGGCGGGCAGGAACGGGGCT
ATCGCGGCGGGACAGAGGCGATGCCTCTGGTCCTGGGCATGGTCGCTGCGCTGGAAGCTGGAAACGGCTGGCTAAAGCGA
GCTTTAGGTTTTCGGGAATCCCTTACGAATCAAATTGTTGATATGGGGGCGGAAATTGTCGCGGGGGATGCACCGCGCAG
CCCGACGATTGCCGCCTATCGCATGCCTGGCAAGTCGAGCGCGGCACAACTGATCCGCTTCGACGGTGCGGGCATCGCGG
TCTCGGCGGGAAGCGCGTGCTCTTCGGGCTCGCTCAAGGCCAGCCATGTCCTGACCGCGATGGGCTATGCGCACCCGGGC
GAAGTGATCCGCGTTTCCATCGGGCGCGAGACGACCGAGGCCGAAATCGCGCGGTTCGTGGAAGTGTGGAAGGGCATTCG
CGGGTGA

Upstream 100 bases:

>100_bases
CGCAGCGGCAAGGACTGCCGGGGCTGGGAATTGCGTTGACGGCTATATAGAAACGACCCCCGCGAAAGCAATCTTTGCAG
ACATTGCCGGATAACCTGAT

Downstream 100 bases:

>100_bases
TCTACCTCGACTACCAGGCGACGACCCCTCTCGCCCCGGAAGCGCGCGCGGCGATGCTGCCGTGGCTGGCGGGGCCCGAG
GCACCGGGATTTGCCAATCC

Product: class V aminotransferase

Products: NA

Alternate protein names: Nitrogenase metalloclusters biosynthesis protein nifS1 [H]

Number of amino acids: Translated: 348; Mature: 347

Protein sequence:

>348_residues
MPSRPIYLDHAATTPLLPEAMAAMEAGWRVWANPSSPHAAGRAARAALEDARARLKAALGWRGEVIFTSGASEALAIGIG
RAKVGQVLVSAVEHDAALRVAGTAPRLPVGADGRVDPNGTERYMSGTDRALYVVQQVNSETGVIQDIAALADRVRSGGHL
LLCDAAQGAGKIDLPDADMVVLSAHKFGGPVGVGALLVRDYGLLEPSGGQERGYRGGTEAMPLVLGMVAALEAGNGWLKR
ALGFRESLTNQIVDMGAEIVAGDAPRSPTIAAYRMPGKSSAAQLIRFDGAGIAVSAGSACSSGSLKASHVLTAMGYAHPG
EVIRVSIGRETTEAEIARFVEVWKGIRG

Sequences:

>Translated_348_residues
MPSRPIYLDHAATTPLLPEAMAAMEAGWRVWANPSSPHAAGRAARAALEDARARLKAALGWRGEVIFTSGASEALAIGIG
RAKVGQVLVSAVEHDAALRVAGTAPRLPVGADGRVDPNGTERYMSGTDRALYVVQQVNSETGVIQDIAALADRVRSGGHL
LLCDAAQGAGKIDLPDADMVVLSAHKFGGPVGVGALLVRDYGLLEPSGGQERGYRGGTEAMPLVLGMVAALEAGNGWLKR
ALGFRESLTNQIVDMGAEIVAGDAPRSPTIAAYRMPGKSSAAQLIRFDGAGIAVSAGSACSSGSLKASHVLTAMGYAHPG
EVIRVSIGRETTEAEIARFVEVWKGIRG
>Mature_347_residues
PSRPIYLDHAATTPLLPEAMAAMEAGWRVWANPSSPHAAGRAARAALEDARARLKAALGWRGEVIFTSGASEALAIGIGR
AKVGQVLVSAVEHDAALRVAGTAPRLPVGADGRVDPNGTERYMSGTDRALYVVQQVNSETGVIQDIAALADRVRSGGHLL
LCDAAQGAGKIDLPDADMVVLSAHKFGGPVGVGALLVRDYGLLEPSGGQERGYRGGTEAMPLVLGMVAALEAGNGWLKRA
LGFRESLTNQIVDMGAEIVAGDAPRSPTIAAYRMPGKSSAAQLIRFDGAGIAVSAGSACSSGSLKASHVLTAMGYAHPGE
VIRVSIGRETTEAEIARFVEVWKGIRG

Specific function: Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Seems to participate in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for the Fe-S core formation [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=371, Percent_Identity=34.2318059299191, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI156713448, Length=413, Percent_Identity=28.3292978208232, Blast_Score=121, Evalue=8e-28,
Organism=Escherichia coli, GI48994898, Length=377, Percent_Identity=35.0132625994695, Blast_Score=145, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI25143064, Length=378, Percent_Identity=32.5396825396825, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17533177, Length=241, Percent_Identity=30.2904564315353, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319831, Length=376, Percent_Identity=32.4468085106383, Blast_Score=131, Evalue=1e-31,
Organism=Drosophila melanogaster, GI20129463, Length=371, Percent_Identity=34.2318059299191, Blast_Score=138, Evalue=6e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 36033; Mature: 35901

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSRPIYLDHAATTPLLPEAMAAMEAGWRVWANPSSPHAAGRAARAALEDARARLKAALG
CCCCCEEEECCCCCCCHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHC
WRGEVIFTSGASEALAIGIGRAKVGQVLVSAVEHDAALRVAGTAPRLPVGADGRVDPNGT
CCCEEEEECCCCCEEEEECCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCH
ERYMSGTDRALYVVQQVNSETGVIQDIAALADRVRSGGHLLLCDAAQGAGKIDLPDADMV
HHHHCCCCCEEEEEEHHCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCEE
VLSAHKFGGPVGVGALLVRDYGLLEPSGGQERGYRGGTEAMPLVLGMVAALEAGNGWLKR
EEEEHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHH
ALGFRESLTNQIVDMGAEIVAGDAPRSPTIAAYRMPGKSSAAQLIRFDGAGIAVSAGSAC
HHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEECCCCCCHHHEEEECCCCEEEECCCCC
SSGSLKASHVLTAMGYAHPGEVIRVSIGRETTEAEIARFVEVWKGIRG
CCCCCHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PSRPIYLDHAATTPLLPEAMAAMEAGWRVWANPSSPHAAGRAARAALEDARARLKAALG
CCCCEEEECCCCCCCHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHC
WRGEVIFTSGASEALAIGIGRAKVGQVLVSAVEHDAALRVAGTAPRLPVGADGRVDPNGT
CCCEEEEECCCCCEEEEECCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCH
ERYMSGTDRALYVVQQVNSETGVIQDIAALADRVRSGGHLLLCDAAQGAGKIDLPDADMV
HHHHCCCCCEEEEEEHHCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCEE
VLSAHKFGGPVGVGALLVRDYGLLEPSGGQERGYRGGTEAMPLVLGMVAALEAGNGWLKR
EEEEHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHH
ALGFRESLTNQIVDMGAEIVAGDAPRSPTIAAYRMPGKSSAAQLIRFDGAGIAVSAGSAC
HHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEECCCCCCHHHEEEECCCCEEEECCCCC
SSGSLKASHVLTAMGYAHPGEVIRVSIGRETTEAEIARFVEVWKGIRG
CCCCCHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7568132 [H]