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The map label for this gene is traC [H]

Identifier: 87200724

GI number: 87200724

Start: 2944445

End: 2945338

Strand: Direct

Name: traC [H]

Synonym: Saro_2711

Alternate gene names: 87200724

Gene position: 2944445-2945338 (Clockwise)

Preceding gene: 87200723

Following gene: 87200725

Centisome position: 82.67

GC content: 65.32

Gene sequence:

>894_bases
ATGAGTATCCACGATTCCATCGCGGACTTCATCGGCTTCATGGAAGCCAATGGCGTTGTGCCTATCGAGCCGATCGCACA
GCGGCTCGCTTCTGGTTCCCTGATCCGGTTCCGCTGCGAAGGGGACGGAAAGGGCCGGCAGAACGGTTGGGCCATCCTCT
ACCTCGACCAGCGCCCCGCCGGCGCCTTCGGCAACTACCGCATGAACACCGGCACCTTGAAGTGGAAGGCGTCGGACGAC
CGCCCGGCCCTGTCCGCAGCGGAGCGCGACGCACTGCAACGCGAGTGGCGTGCGGCGAAGGAGCGGCGCGAGGCCGAGCA
GCACAACAACCAACGCCAGGCCGCGTTAGCCGCAGCCGACCTGTGGCAGCGCGCCAGCGCCGCGACGCCCGAACATCCGT
ACGTCGCGCGCAAGCAGATCGATCCATCGCCCCTGCGCGTCCTCGGCGAAGAACTGTTGATACCGATGACCGACAACACC
GGCCTGCTGTGGAACTTGCAGCGCATCAAGCCGGACGGCGAGAAGCGATTCCTAAAGGGCGGCCGCATCAACGGCCTGTT
CGCGATCGTCGGAAGGTTCTCGCCCGAGACAGAAGAGGCCGTGATCTGCGAGGGCTATGCCACTGCGGACACCGTGCACC
AGGCAACTGGATTGCCGGTGATCGTGACCTTCAACACGTCGAACATGGCCCGCGTCGCGCGGCTCTTCGCCGAGAAGCGG
CCGGACCTGAGCTACACGGTTTTCGCTGACGACGATGAAGCGACCGCACTGCGCGAGATGGAGCATCGCGGCATCTACAA
GAATCCCGGGATCGAGACCGCCGAAGCCGTTGCTGCCGAGATCGGAGCGCACGTTGCCTATCCGCTGGGTCGGCCGCAGA
GGGGGGCAGCGTGA

Upstream 100 bases:

>100_bases
CCGCCGAGATGAAGGCCCGGCTCCTCATCGCCCGCGAACACGGCCACCTCGACGACGAGGCGGTCGAAGACCTGATCATC
TTCCACGGGCTGCAAAACGC

Downstream 100 bases:

>100_bases
GCGTGTCGGACGCAGGCAACTTTGACGCAAACGACGTCGCCGTCACCTTCGGCATCGACAAGGTGCGCGAGGCGTTCGAG
CAGCGCCGTAGCACCCTGCC

Product: hypothetical protein

Products: NA

Alternate protein names: Replication primase [H]

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MSIHDSIADFIGFMEANGVVPIEPIAQRLASGSLIRFRCEGDGKGRQNGWAILYLDQRPAGAFGNYRMNTGTLKWKASDD
RPALSAAERDALQREWRAAKERREAEQHNNQRQAALAAADLWQRASAATPEHPYVARKQIDPSPLRVLGEELLIPMTDNT
GLLWNLQRIKPDGEKRFLKGGRINGLFAIVGRFSPETEEAVICEGYATADTVHQATGLPVIVTFNTSNMARVARLFAEKR
PDLSYTVFADDDEATALREMEHRGIYKNPGIETAEAVAAEIGAHVAYPLGRPQRGAA

Sequences:

>Translated_297_residues
MSIHDSIADFIGFMEANGVVPIEPIAQRLASGSLIRFRCEGDGKGRQNGWAILYLDQRPAGAFGNYRMNTGTLKWKASDD
RPALSAAERDALQREWRAAKERREAEQHNNQRQAALAAADLWQRASAATPEHPYVARKQIDPSPLRVLGEELLIPMTDNT
GLLWNLQRIKPDGEKRFLKGGRINGLFAIVGRFSPETEEAVICEGYATADTVHQATGLPVIVTFNTSNMARVARLFAEKR
PDLSYTVFADDDEATALREMEHRGIYKNPGIETAEAVAAEIGAHVAYPLGRPQRGAA
>Mature_296_residues
SIHDSIADFIGFMEANGVVPIEPIAQRLASGSLIRFRCEGDGKGRQNGWAILYLDQRPAGAFGNYRMNTGTLKWKASDDR
PALSAAERDALQREWRAAKERREAEQHNNQRQAALAAADLWQRASAATPEHPYVARKQIDPSPLRVLGEELLIPMTDNTG
LLWNLQRIKPDGEKRFLKGGRINGLFAIVGRFSPETEEAVICEGYATADTVHQATGLPVIVTFNTSNMARVARLFAEKRP
DLSYTVFADDDEATALREMEHRGIYKNPGIETAEAVAAEIGAHVAYPLGRPQRGAA

Specific function: Required for autonomous replication in E.coli. Transferred into the recipient cell during bacterial conjugation. Catalyzes the synthesis of short oligoribonucleotide primers with CpA or pCpA at their 5'-termini on a single stranded template DNA [H]

COG id: COG4643

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013610
- InterPro:   IPR006171 [H]

Pfam domain/function: PF08401 DUF1738; PF01751 Toprim [H]

EC number: NA

Molecular weight: Translated: 32764; Mature: 32633

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIHDSIADFIGFMEANGVVPIEPIAQRLASGSLIRFRCEGDGKGRQNGWAILYLDQRPA
CCHHHHHHHHHHHHHCCCCEEHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEEEEECCCC
GAFGNYRMNTGTLKWKASDDRPALSAAERDALQREWRAAKERREAEQHNNQRQAALAAAD
CCCCCEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
LWQRASAATPEHPYVARKQIDPSPLRVLGEELLIPMTDNTGLLWNLQRIKPDGEKRFLKG
HHHHHCCCCCCCCCCHHHCCCCCHHHHHHHHHEEEEECCCCEEEEEHCCCCCCHHHHHCC
GRINGLFAIVGRFSPETEEAVICEGYATADTVHQATGLPVIVTFNTSNMARVARLFAEKR
CCCCEEEEEEECCCCCCCCEEEECCCCCHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCC
PDLSYTVFADDDEATALREMEHRGIYKNPGIETAEAVAAEIGAHVAYPLGRPQRGAA
CCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCEECCCCCCCCCCC
>Mature Secondary Structure 
SIHDSIADFIGFMEANGVVPIEPIAQRLASGSLIRFRCEGDGKGRQNGWAILYLDQRPA
CHHHHHHHHHHHHHCCCCEEHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEEEEECCCC
GAFGNYRMNTGTLKWKASDDRPALSAAERDALQREWRAAKERREAEQHNNQRQAALAAAD
CCCCCEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
LWQRASAATPEHPYVARKQIDPSPLRVLGEELLIPMTDNTGLLWNLQRIKPDGEKRFLKG
HHHHHCCCCCCCCCCHHHCCCCCHHHHHHHHHEEEEECCCCEEEEEHCCCCCCHHHHHCC
GRINGLFAIVGRFSPETEEAVICEGYATADTVHQATGLPVIVTFNTSNMARVARLFAEKR
CCCCEEEEEEECCCCCCCCEEEECCCCCHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCC
PDLSYTVFADDDEATALREMEHRGIYKNPGIETAEAVAAEIGAHVAYPLGRPQRGAA
CCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1818755 [H]