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The map label for this gene is btuB [H]

Identifier: 87200471

GI number: 87200471

Start: 2649885

End: 2652161

Strand: Reverse

Name: btuB [H]

Synonym: Saro_2458

Alternate gene names: 87200471

Gene position: 2652161-2649885 (Counterclockwise)

Preceding gene: 87200472

Following gene: 87200470

Centisome position: 74.47

GC content: 60.87

Gene sequence:

>2277_bases
ATGAAGCGACGTCTTCGGGCAATTTGCCTTGCGCCTCTGGGGGCCGCACTGGTCCTCCCGGCAGCCGCCCACGCACAGTC
GGCGCAGGCCGGTGATGCAGCCGACTCCTCGGCTCCTTCCACAGCCGAAATCATTGTCACCGCAACGAAGCAGAATACCG
CGCTCAGCCGCGTTGCTGCTGCCGTTACTGCCGTGACTGCGGCGGATATCGGTCCCGGCGGCGTCCAGAACGTGGGAGAC
CTGCAGGTTGTCGTGCCGAACGTTTCGATCGGCGATCAGTTCGGCGTCAACCGTACCTTCATTCGCGGCGTCGGCCTGAA
CAACTTCGATGTCGGCGGGGAAGGCGCGGTCGCGTTCCTCCAGAACGGCGCAATCCTGGCGCGTCCGGCCCAGCAGCTCA
GTGGCTTCTTCGACCTTTCCCAGATCGAAGTCCTGCGCGGACCGCAGTCGATCCTTTACGGTCGCGGCGCCACTGCTGGC
GCGATCAATCTCGTTACCGCAGCTCCGACCGACGAGATGGACGGGTATCTGCGAGCCACCTATGGCAACTATGACAACCG
GGTGCTTGAAGGCGCGATCGGCGGTCCGCTCGCGGGCGACCGCTTGATGGTGCGCCTCGCCGGCAAGTATGAAAAGCGCG
ACGGTTACGGCGTCAACCTTTTCACCGGTAATCCGGTCGATGACCGCGATGCCTATTCCTTGCGCGCTACCGTGGTCGCC
AAGCTCAGCGATACGTTCAAGGCGACGATCGTTGGCGATCATTTCAAGGAAGACGACAACAATTATGCCTTCCACTATTT
CGGTCCGTCGGTAATTCCCGAAGCAGGGCTGTTCCACAAGTTCATCGGTGGCAAGTCGATCTTCGACTATTACGCCGCGC
GCAACGAAAAGCCCAACCTGCGCAATATCTATTCCGATGAAGAGGCGATCAACAACCGCAAGGGCTATGGCGTGACCGGC
ACGCTCGACTGGGACCTGGGCGCTGCATCGGTCAAGTCGATCACCGCCTACCGCACATTCGACCGTTTCCAGCGCGACGA
CCTCGACGTGTCCGACGTCAATCTTGCCGGCCAGAACAACTACGTCGAAAAGAGCCGCGCCTTCAGCCAGGAAGTCACAG
TCAACTACGAAGGCGATGGTTTCACACTGCTCGGCGGTGCGATGTACCTGCACGAGAAGTTGACTGGGCAGGTGCTCGTG
CCGACCGTGAACCTCGGCGTCCTGTTCGGCCTGCCGGCCGATACGTTCGACGATGGCGCCTACGAGCAGAACGGACTGGT
CAAGATCGATGCCGTCGGCGTCTATCTCCAGGGTGCGGTCGATCTCAGCCCCACGATCAAGCTCACCGCCGGCGCGCGCT
ACAACTATGAGCATCGCAAGGGTGATGGCTACTTCCGCTTCGATGCGCTCGGCGTGAACATTCCGACCGACAAGTCCAAG
GGTTGGTCCTCGGTTACGCCGAAGGTGTTGCTGGAGTTCAAGCCGACTGACCGCACGCTCCTCTACGCAAGCGTCACAAA
GGGCTTCAAGTCGGGCGTCATCAACATCGGCAGCGTCGATGCGGCGATCGATCCAGAAACGGTCTGGAGCTACGAAGCGG
GCTTCAAACAGAAGCTGGCGGACAATCGCGTCCTGCTCAGCGGTGCGGTGTTCTACTACGATTACAAGAACCTGCAGGTC
AGCTTCGTCAACGCCAACTCGATCGTGCAGACGATCAATGCGGCAAACGCGCGCAACTACGGCGGCGAGCTGGAGCTTGA
AGGCAAGATCACGCCGCAGTTCTCCGTGAACCTCAACGCGTCCTACCTCAATGCTCAGTTCACGAAGTTCTGCAACGCCT
ACTACGGCGCGGCCTTCCCGGCGCGGTCCGGCATTTCCTATCCGGTTTGCCCCACCGATTCCGGCCTCGTCGACCTCAGC
GGAAAGCGCCTTCCGAACGCGCCGCGCTTCACCGTTGGCGGTGGCTTCAACTGGGACATCCCGCTGGGCAACGACAGCCG
CCTTGCCGTCAACGGCGAGATCAAGTGGCAGTCGAAGGTCTACTTCACCGAGTTCAACAATCGCGATGCGGAACAAGGTT
CCTATGCCATGGCGAATGCCGGTCTGACCTGGCACGCACCGGGCGACCGCTTCTCGATCGGCGGGTGGGTGAAAAACATC
ACCAACGAGTTCGTGATTGCGAACAACATCATCACTGCCGCCACGTTCGCCTATGTCCGCGTCGGCTCGGTCATGCCGCC
GCGCACCTACGGGGTGACTGCCAGCGTGAATTTCTGA

Upstream 100 bases:

>100_bases
TCGCCTGGGCCACCCTATTGATTACCAAGGGAATGCAAGATATACCCGTGAGTATGAAAACGGCGCCGTGAGACAGGCGC
CTATACAAGGGGGAGTTCAG

Downstream 100 bases:

>100_bases
CCCCTGGAAGGAGGCGAGATGGACCGCTTTGACCTCGGAATCGTCAAGGTTCATCGTATCGAGGAATGGCGCGGGACATT
CTCGCCTCCAGCCGAACTTT

Product: TonB-dependent receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 758; Mature: 758

Protein sequence:

>758_residues
MKRRLRAICLAPLGAALVLPAAAHAQSAQAGDAADSSAPSTAEIIVTATKQNTALSRVAAAVTAVTAADIGPGGVQNVGD
LQVVVPNVSIGDQFGVNRTFIRGVGLNNFDVGGEGAVAFLQNGAILARPAQQLSGFFDLSQIEVLRGPQSILYGRGATAG
AINLVTAAPTDEMDGYLRATYGNYDNRVLEGAIGGPLAGDRLMVRLAGKYEKRDGYGVNLFTGNPVDDRDAYSLRATVVA
KLSDTFKATIVGDHFKEDDNNYAFHYFGPSVIPEAGLFHKFIGGKSIFDYYAARNEKPNLRNIYSDEEAINNRKGYGVTG
TLDWDLGAASVKSITAYRTFDRFQRDDLDVSDVNLAGQNNYVEKSRAFSQEVTVNYEGDGFTLLGGAMYLHEKLTGQVLV
PTVNLGVLFGLPADTFDDGAYEQNGLVKIDAVGVYLQGAVDLSPTIKLTAGARYNYEHRKGDGYFRFDALGVNIPTDKSK
GWSSVTPKVLLEFKPTDRTLLYASVTKGFKSGVINIGSVDAAIDPETVWSYEAGFKQKLADNRVLLSGAVFYYDYKNLQV
SFVNANSIVQTINAANARNYGGELELEGKITPQFSVNLNASYLNAQFTKFCNAYYGAAFPARSGISYPVCPTDSGLVDLS
GKRLPNAPRFTVGGGFNWDIPLGNDSRLAVNGEIKWQSKVYFTEFNNRDAEQGSYAMANAGLTWHAPGDRFSIGGWVKNI
TNEFVIANNIITAATFAYVRVGSVMPPRTYGVTASVNF

Sequences:

>Translated_758_residues
MKRRLRAICLAPLGAALVLPAAAHAQSAQAGDAADSSAPSTAEIIVTATKQNTALSRVAAAVTAVTAADIGPGGVQNVGD
LQVVVPNVSIGDQFGVNRTFIRGVGLNNFDVGGEGAVAFLQNGAILARPAQQLSGFFDLSQIEVLRGPQSILYGRGATAG
AINLVTAAPTDEMDGYLRATYGNYDNRVLEGAIGGPLAGDRLMVRLAGKYEKRDGYGVNLFTGNPVDDRDAYSLRATVVA
KLSDTFKATIVGDHFKEDDNNYAFHYFGPSVIPEAGLFHKFIGGKSIFDYYAARNEKPNLRNIYSDEEAINNRKGYGVTG
TLDWDLGAASVKSITAYRTFDRFQRDDLDVSDVNLAGQNNYVEKSRAFSQEVTVNYEGDGFTLLGGAMYLHEKLTGQVLV
PTVNLGVLFGLPADTFDDGAYEQNGLVKIDAVGVYLQGAVDLSPTIKLTAGARYNYEHRKGDGYFRFDALGVNIPTDKSK
GWSSVTPKVLLEFKPTDRTLLYASVTKGFKSGVINIGSVDAAIDPETVWSYEAGFKQKLADNRVLLSGAVFYYDYKNLQV
SFVNANSIVQTINAANARNYGGELELEGKITPQFSVNLNASYLNAQFTKFCNAYYGAAFPARSGISYPVCPTDSGLVDLS
GKRLPNAPRFTVGGGFNWDIPLGNDSRLAVNGEIKWQSKVYFTEFNNRDAEQGSYAMANAGLTWHAPGDRFSIGGWVKNI
TNEFVIANNIITAATFAYVRVGSVMPPRTYGVTASVNF
>Mature_758_residues
MKRRLRAICLAPLGAALVLPAAAHAQSAQAGDAADSSAPSTAEIIVTATKQNTALSRVAAAVTAVTAADIGPGGVQNVGD
LQVVVPNVSIGDQFGVNRTFIRGVGLNNFDVGGEGAVAFLQNGAILARPAQQLSGFFDLSQIEVLRGPQSILYGRGATAG
AINLVTAAPTDEMDGYLRATYGNYDNRVLEGAIGGPLAGDRLMVRLAGKYEKRDGYGVNLFTGNPVDDRDAYSLRATVVA
KLSDTFKATIVGDHFKEDDNNYAFHYFGPSVIPEAGLFHKFIGGKSIFDYYAARNEKPNLRNIYSDEEAINNRKGYGVTG
TLDWDLGAASVKSITAYRTFDRFQRDDLDVSDVNLAGQNNYVEKSRAFSQEVTVNYEGDGFTLLGGAMYLHEKLTGQVLV
PTVNLGVLFGLPADTFDDGAYEQNGLVKIDAVGVYLQGAVDLSPTIKLTAGARYNYEHRKGDGYFRFDALGVNIPTDKSK
GWSSVTPKVLLEFKPTDRTLLYASVTKGFKSGVINIGSVDAAIDPETVWSYEAGFKQKLADNRVLLSGAVFYYDYKNLQV
SFVNANSIVQTINAANARNYGGELELEGKITPQFSVNLNASYLNAQFTKFCNAYYGAAFPARSGISYPVCPTDSGLVDLS
GKRLPNAPRFTVGGGFNWDIPLGNDSRLAVNGEIKWQSKVYFTEFNNRDAEQGSYAMANAGLTWHAPGDRFSIGGWVKNI
TNEFVIANNIITAATFAYVRVGSVMPPRTYGVTASVNF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG1629

COG function: function code P; Outer membrane receptor proteins, mostly Fe transport

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010916 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 81611; Mature: 81611

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRRLRAICLAPLGAALVLPAAAHAQSAQAGDAADSSAPSTAEIIVTATKQNTALSRVAA
CCCCEEEEEECCCCHHHHCCCHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHH
AVTAVTAADIGPGGVQNVGDLQVVVPNVSIGDQFGVNRTFIRGVGLNNFDVGGEGAVAFL
HHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEE
QNGAILARPAQQLSGFFDLSQIEVLRGPQSILYGRGATAGAINLVTAAPTDEMDGYLRAT
ECCEEEECCHHHHCCCCCHHHHHHHCCCHHHEECCCCCCCEEEEEEECCCCCCCCEEEEE
YGNYDNRVLEGAIGGPLAGDRLMVRLAGKYEKRDGYGVNLFTGNPVDDRDAYSLRATVVA
CCCCCCEEEECCCCCCCCCCEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEE
KLSDTFKATIVGDHFKEDDNNYAFHYFGPSVIPEAGLFHKFIGGKSIFDYYAARNEKPNL
ECCCCEEEEEEECCCCCCCCCEEEEEECCCCCCCCCHHHHHHCCCHHHHHHHHCCCCCCC
RNIYSDEEAINNRKGYGVTGTLDWDLGAASVKSITAYRTFDRFQRDDLDVSDVNLAGQNN
CCCCCCHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCC
YVEKSRAFSQEVTVNYEGDGFTLLGGAMYLHEKLTGQVLVPTVNLGVLFGLPADTFDDGA
CHHHHHCCCEEEEEEECCCCEEEECCCEEEHHHCCCCEEEEEECEEEEEECCCCCCCCCC
YEQNGLVKIDAVGVYLQGAVDLSPTIKLTAGARYNYEHRKGDGYFRFDALGVNIPTDKSK
CCCCCEEEEEEEEEEEEEEECCCCEEEEEECCEECCCCCCCCCEEEEEEEEEECCCCCCC
GWSSVTPKVLLEFKPTDRTLLYASVTKGFKSGVINIGSVDAAIDPETVWSYEAGFKQKLA
CCCCCCCEEEEEECCCCCEEEEEEHHHHHHCCCEEECCCCCCCCCHHHEEECCCHHHHHC
DNRVLLSGAVFYYDYKNLQVSFVNANSIVQTINAANARNYGGELELEGKITPQFSVNLNA
CCEEEEEEEEEEEEECCEEEEEEECHHHHHHHHCCCCCCCCCEEEEEEEECCEEEEEECH
SYLNAQFTKFCNAYYGAAFPARSGISYPVCPTDSGLVDLSGKRLPNAPRFTVGGGFNWDI
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCEEEECCCEEEEE
PLGNDSRLAVNGEIKWQSKVYFTEFNNRDAEQGSYAMANAGLTWHAPGDRFSIGGWVKNI
ECCCCCEEEEECEEEEEEEEEEEECCCCCCCCCCEEEEECCEEECCCCCCEECCHHHHHC
TNEFVIANNIITAATFAYVRVGSVMPPRTYGVTASVNF
CCCEEEECCHHHHHHEEHHEECCCCCCCCCEEEEEECC
>Mature Secondary Structure
MKRRLRAICLAPLGAALVLPAAAHAQSAQAGDAADSSAPSTAEIIVTATKQNTALSRVAA
CCCCEEEEEECCCCHHHHCCCHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHH
AVTAVTAADIGPGGVQNVGDLQVVVPNVSIGDQFGVNRTFIRGVGLNNFDVGGEGAVAFL
HHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEE
QNGAILARPAQQLSGFFDLSQIEVLRGPQSILYGRGATAGAINLVTAAPTDEMDGYLRAT
ECCEEEECCHHHHCCCCCHHHHHHHCCCHHHEECCCCCCCEEEEEEECCCCCCCCEEEEE
YGNYDNRVLEGAIGGPLAGDRLMVRLAGKYEKRDGYGVNLFTGNPVDDRDAYSLRATVVA
CCCCCCEEEECCCCCCCCCCEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEE
KLSDTFKATIVGDHFKEDDNNYAFHYFGPSVIPEAGLFHKFIGGKSIFDYYAARNEKPNL
ECCCCEEEEEEECCCCCCCCCEEEEEECCCCCCCCCHHHHHHCCCHHHHHHHHCCCCCCC
RNIYSDEEAINNRKGYGVTGTLDWDLGAASVKSITAYRTFDRFQRDDLDVSDVNLAGQNN
CCCCCCHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCC
YVEKSRAFSQEVTVNYEGDGFTLLGGAMYLHEKLTGQVLVPTVNLGVLFGLPADTFDDGA
CHHHHHCCCEEEEEEECCCCEEEECCCEEEHHHCCCCEEEEEECEEEEEECCCCCCCCCC
YEQNGLVKIDAVGVYLQGAVDLSPTIKLTAGARYNYEHRKGDGYFRFDALGVNIPTDKSK
CCCCCEEEEEEEEEEEEEEECCCCEEEEEECCEECCCCCCCCCEEEEEEEEEECCCCCCC
GWSSVTPKVLLEFKPTDRTLLYASVTKGFKSGVINIGSVDAAIDPETVWSYEAGFKQKLA
CCCCCCCEEEEEECCCCCEEEEEEHHHHHHCCCEEECCCCCCCCCHHHEEECCCHHHHHC
DNRVLLSGAVFYYDYKNLQVSFVNANSIVQTINAANARNYGGELELEGKITPQFSVNLNA
CCEEEEEEEEEEEEECCEEEEEEECHHHHHHHHCCCCCCCCCEEEEEEEECCEEEEEECH
SYLNAQFTKFCNAYYGAAFPARSGISYPVCPTDSGLVDLSGKRLPNAPRFTVGGGFNWDI
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCEEEECCCEEEEE
PLGNDSRLAVNGEIKWQSKVYFTEFNNRDAEQGSYAMANAGLTWHAPGDRFSIGGWVKNI
ECCCCCEEEEECEEEEEEEEEEEECCCCCCCCCCEEEEECCEEECCCCCCEECCHHHHHC
TNEFVIANNIITAATFAYVRVGSVMPPRTYGVTASVNF
CCCEEEECCHHHHHHEEHHEECCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA