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The map label for this gene is prfB [H]

Identifier: 87200328

GI number: 87200328

Start: 2454382

End: 2455509

Strand: Reverse

Name: prfB [H]

Synonym: Saro_2314

Alternate gene names: 87200328

Gene position: 2455509-2454382 (Counterclockwise)

Preceding gene: 87200329

Following gene: 87200327

Centisome position: 68.94

GC content: 66.22

Gene sequence:

>1128_bases
ATGCGTGCCGAAGGGCAGGCCCATATCGACCGCATCGAGGCCGCACTGGCGCTGGTGCGCAAGTTCCTCGACTGGGACCG
CGCGGTTCGCCGCCTTGACGAGCTGAACGCGCGCGTCGAGGACCCCAAGCTGTGGGACGATCCCAAGAAGGCCGAGGAAG
TGATGCGCGAGCGTCGCCGGCTGGAAGCCTCGATCGGCACCGTCAACCAGATCGGCCAGGAAATGGCCGATGCCATCGAG
TTCGTCGAACTCGGCGAAATGGAAGGCGACGAGGACACGATCAACGAAGGCCTCGCGACGCTTGCCGCGCTGGCCGAGCG
TGCCGACAACGACAAGGTCCAGGCCCTGCTGGCGGGCGAGGCTGATGCCAACAACGCCTTCCTCGAAGTTCACGCGGGCG
CGGGCGGCACCGAAAGCCAGGACTGGGCCGAGATGCTCCAGCGCATGTACACGCGCTGGGCCGAGCGGCACGGATACAAG
GTTGAACTGGTCGACTATCACGCTGGCGAGGCTGCCGGGATCAAGTCGGCCACGCTGCTGATCAAGGGCGAGAACGCCTA
TGGCTATGCCAAGACCGAGAGCGGCGTGCACCGCCTCGTGCGCATCAGCCCCTATGACAGCTCGGCGCGGCGCCACACCT
CGTTCAGCTCGGTCTGGGTCTATCCCGAGATCGACGACAACATCGAGGTCGAGATCAATCCGGCCGACCTCAAGATCGAC
ACCTACCGCGCGTCGGGCGCGGGTGGGCAGCACGTGAACACGACCGACTCCGCGGTCCGCATCACCCACGTGCCCACCGG
CATCATCGTCGCCAGCCAGAACGACCGCAGCCAGCACAAGAACCGCGCAACCGCGATGGGCATGCTGAAGGCGCGCATCT
ACGAGGCGGAACTGGCCAAGCGCGAGGCCGCGGCCAGCGGCGAATATGCGGCCAAGACCGAGATCGGCTGGGGCCACCAG
ATCCGCTCCTACGTGTTGCAGCCCTACCAGCAGGTGAAGGACCTGCGCACGGGCGTTGTCTCCACCAATCCCACCGAAGT
GCTCGACGGCGCGCTCGACCCGTTCATGGCGGCGGCGCTTTCGCAGCGCGTGACCGGCGAGAAGGTGACGGTGGAGGACG
ACGATTGA

Upstream 100 bases:

>100_bases
CGCGCGCTTACTGACACACGGTTGGGCTTTACATGCCTGCCCGCTTCGCTAAGCGGCGCAGTTCATATCGTGTTTCGCGT
TTGATCGGAGTTGGATTGCC

Downstream 100 bases:

>100_bases
GCAGGGCTGCGGCCCTCTCCTCTCTTGCGCTGGTGGCGCTGCTCGCCGCCTGCAAGCCGGCGCCGCAGGACGACCGGCTC
GAAACCGCGCGCGGCTTCCC

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MRAEGQAHIDRIEAALALVRKFLDWDRAVRRLDELNARVEDPKLWDDPKKAEEVMRERRRLEASIGTVNQIGQEMADAIE
FVELGEMEGDEDTINEGLATLAALAERADNDKVQALLAGEADANNAFLEVHAGAGGTESQDWAEMLQRMYTRWAERHGYK
VELVDYHAGEAAGIKSATLLIKGENAYGYAKTESGVHRLVRISPYDSSARRHTSFSSVWVYPEIDDNIEVEINPADLKID
TYRASGAGGQHVNTTDSAVRITHVPTGIIVASQNDRSQHKNRATAMGMLKARIYEAELAKREAAASGEYAAKTEIGWGHQ
IRSYVLQPYQQVKDLRTGVVSTNPTEVLDGALDPFMAAALSQRVTGEKVTVEDDD

Sequences:

>Translated_375_residues
MRAEGQAHIDRIEAALALVRKFLDWDRAVRRLDELNARVEDPKLWDDPKKAEEVMRERRRLEASIGTVNQIGQEMADAIE
FVELGEMEGDEDTINEGLATLAALAERADNDKVQALLAGEADANNAFLEVHAGAGGTESQDWAEMLQRMYTRWAERHGYK
VELVDYHAGEAAGIKSATLLIKGENAYGYAKTESGVHRLVRISPYDSSARRHTSFSSVWVYPEIDDNIEVEINPADLKID
TYRASGAGGQHVNTTDSAVRITHVPTGIIVASQNDRSQHKNRATAMGMLKARIYEAELAKREAAASGEYAAKTEIGWGHQ
IRSYVLQPYQQVKDLRTGVVSTNPTEVLDGALDPFMAAALSQRVTGEKVTVEDDD
>Mature_375_residues
MRAEGQAHIDRIEAALALVRKFLDWDRAVRRLDELNARVEDPKLWDDPKKAEEVMRERRRLEASIGTVNQIGQEMADAIE
FVELGEMEGDEDTINEGLATLAALAERADNDKVQALLAGEADANNAFLEVHAGAGGTESQDWAEMLQRMYTRWAERHGYK
VELVDYHAGEAAGIKSATLLIKGENAYGYAKTESGVHRLVRISPYDSSARRHTSFSSVWVYPEIDDNIEVEINPADLKID
TYRASGAGGQHVNTTDSAVRITHVPTGIIVASQNDRSQHKNRATAMGMLKARIYEAELAKREAAASGEYAAKTEIGWGHQ
IRSYVLQPYQQVKDLRTGVVSTNPTEVLDGALDPFMAAALSQRVTGEKVTVEDDD

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=221, Percent_Identity=42.0814479638009, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI34577120, Length=280, Percent_Identity=36.4285714285714, Blast_Score=151, Evalue=9e-37,
Organism=Homo sapiens, GI166795305, Length=147, Percent_Identity=45.578231292517, Blast_Score=127, Evalue=1e-29,
Organism=Escherichia coli, GI2367172, Length=343, Percent_Identity=52.7696793002915, Blast_Score=386, Evalue=1e-108,
Organism=Escherichia coli, GI1787462, Length=238, Percent_Identity=47.0588235294118, Blast_Score=203, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI17542784, Length=224, Percent_Identity=33.0357142857143, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6321295, Length=262, Percent_Identity=35.1145038167939, Blast_Score=143, Evalue=5e-35,
Organism=Drosophila melanogaster, GI19921226, Length=227, Percent_Identity=40.5286343612335, Blast_Score=158, Evalue=5e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 41465; Mature: 41465

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAEGQAHIDRIEAALALVRKFLDWDRAVRRLDELNARVEDPKLWDDPKKAEEVMRERRR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
LEASIGTVNQIGQEMADAIEFVELGEMEGDEDTINEGLATLAALAERADNDKVQALLAGE
HHHHHCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECC
ADANNAFLEVHAGAGGTESQDWAEMLQRMYTRWAERHGYKVELVDYHAGEAAGIKSATLL
CCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEE
IKGENAYGYAKTESGVHRLVRISPYDSSARRHTSFSSVWVYPEIDDNIEVEINPADLKID
EECCCCCCCCCCHHCCEEEEEECCCCCCHHHCCCCCEEEEEECCCCCEEEEEECCCEEEE
TYRASGAGGQHVNTTDSAVRITHVPTGIIVASQNDRSQHKNRATAMGMLKARIYEAELAK
EEECCCCCCCCCCCCCCEEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
REAAASGEYAAKTEIGWGHQIRSYVLQPYQQVKDLRTGVVSTNPTEVLDGALDPFMAAAL
HHHHCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
SQRVTGEKVTVEDDD
HHHCCCCEEEECCCC
>Mature Secondary Structure
MRAEGQAHIDRIEAALALVRKFLDWDRAVRRLDELNARVEDPKLWDDPKKAEEVMRERRR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
LEASIGTVNQIGQEMADAIEFVELGEMEGDEDTINEGLATLAALAERADNDKVQALLAGE
HHHHHCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECC
ADANNAFLEVHAGAGGTESQDWAEMLQRMYTRWAERHGYKVELVDYHAGEAAGIKSATLL
CCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEE
IKGENAYGYAKTESGVHRLVRISPYDSSARRHTSFSSVWVYPEIDDNIEVEINPADLKID
EECCCCCCCCCCHHCCEEEEEECCCCCCHHHCCCCCEEEEEECCCCCEEEEEECCCEEEE
TYRASGAGGQHVNTTDSAVRITHVPTGIIVASQNDRSQHKNRATAMGMLKARIYEAELAK
EEECCCCCCCCCCCCCCEEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
REAAASGEYAAKTEIGWGHQIRSYVLQPYQQVKDLRTGVVSTNPTEVLDGALDPFMAAAL
HHHHCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
SQRVTGEKVTVEDDD
HHHCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA