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The map label for this gene is nuoN [H]

Identifier: 87200301

GI number: 87200301

Start: 2428043

End: 2429506

Strand: Reverse

Name: nuoN [H]

Synonym: Saro_2287

Alternate gene names: 87200301

Gene position: 2429506-2428043 (Counterclockwise)

Preceding gene: 87200302

Following gene: 87200300

Centisome position: 68.21

GC content: 64.28

Gene sequence:

>1464_bases
ATGGATATCGCCCGTTCGCTCGCGCTCACCGCGCCCGAGGTTCTGATTTCGATCTCGGGGCTGGTGCTGCTGCTGATTGC
CGCCTGGGGCGGTGACAAGGCCTCGCGCCTGATCTCTATCCTGGCCGTTGCGGTGCTCGCCGCATGTGCCGTGCTTGCGG
CGCCCGCGTTGTGGGGCCAGGCCATGGGGGCAGACGTCGCCGCGTTCGGTGGCCAGCACCACGCCGACGCATTTGCCGCG
TTTGCCAAGCTTCTGATCTATGCCAGCGCAGGCGTCACGCTGATCGTCGCGCAGTCGTTCTTCGACAAGGCGAAGGCCAT
GCGTGCGGAATTCCCGCTGCTCGTGCTGTTCGCATCGCTCGGCATGGGCATGATGGTTTCCGCCGGCGATCTGCTGGCGC
TCTACATCGGCCTCGAGCTGAGCAGCCTCGCCAGCTACGTGATGGCGGCATTCCTGCGGACCGACGACCGTTCGGCGGAA
TCGGGCCTCAAGTACTTCGTGCTTGGCTCGCTGGCGTCGGGCATTCTGCTCTTCGGCATGAGCCTGACCTATGGCTTCAC
CGGTACGACCAGCTTTGCCGGCATCAATGCGGCACTTTCCGGCGGTCTCTCGACTGGCGCGACGTTCGGCGTGATCTTCG
TGCTCGCGGGCCTTGCCTTCAAGATCAGCGCCGCGCCGTTCCACATGTGGACGCCCGACGTCTATGAAGGCGCTCCCACG
CCGGTGACGACCTTCTTCGCCACGGCGTCGAAGGTCTCCGCACTTGGCTTGCTGATGCGCGTGAGCTTCGAGGCGTTCGG
CAGTCAGCCGCATGCCTGGCAGCAGGTGGTGATCTTTGCTTCTCTGCTTTCGATCATCGTCGGCGCACTCGGCGCGATCG
GGCAGAACAACATCAAGCGCCTGATGGCCTATTCGTCGATCAACAACGTCGGCTTCATCCTCATCGGCCTTGCAACCGGA
ACGAAGGCCGGTGCTTCGGCCATGCTCGTCTATCTCGCGATTTACGTCGCGATGTCGGTGGCCGGTTTCGTCGCGGTGCT
GCTGCTCCGGGACGAGAACGGGGAGCAGGTCGAGGCGATTTCCGACCTTGCCGGCCTTTCGAAGACGCGCCCGTGGCTGG
CTCTGGCACTCGCTTCGGTCATGTTCAGCCTTGCCGGCATCCCGCCGCTGTTCGGCTTCTGGGGCAAGTTCGTGGTGTTC
CAGGCCGCAGTCCAGGCCGACATGATCGTGCTGGCCGCACTCGGCATCGCAGCCTCCGTGATCGGCGCCTTCTACTACCT
GAAGGTCGTGAAGGTCATGTACTTCGACGAGGCCGTGGACAAGGTGAGGGGCGAGGGCGAACTTTCGCACGCGGTTCTCC
TCGCGCTGTCGACGCTGGCGATTTCTCCGCTGGGGTACCTGGGCAGCAAGTGCCTGGGCACGCTGGCGGACTATGCGGCC
TCCGCGCTGTTCCACGTCGGATGA

Upstream 100 bases:

>100_bases
CCCGTCTCGCGCAGGCGAAGCCCAAGGGGGACGCGCAGGTCGCAGTCGGGAAGGCCAAGCCTGCAACCGAAGAAAATTCC
GCGCATGAGGGGGCGCACTG

Downstream 100 bases:

>100_bases
TCCATTGATCGCAACCGTTGCTGAAATCCCTTCGACCAATGCCGCGCTTCTGGCGCGGCTTGGCGGGGGAGAACTGCTCA
ACGAAGGCGATTGGCTGGTT

Product: NADH dehydrogenase subunit N

Products: NA

Alternate protein names: NADH dehydrogenase I subunit N; NDH-1 subunit N [H]

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MDIARSLALTAPEVLISISGLVLLLIAAWGGDKASRLISILAVAVLAACAVLAAPALWGQAMGADVAAFGGQHHADAFAA
FAKLLIYASAGVTLIVAQSFFDKAKAMRAEFPLLVLFASLGMGMMVSAGDLLALYIGLELSSLASYVMAAFLRTDDRSAE
SGLKYFVLGSLASGILLFGMSLTYGFTGTTSFAGINAALSGGLSTGATFGVIFVLAGLAFKISAAPFHMWTPDVYEGAPT
PVTTFFATASKVSALGLLMRVSFEAFGSQPHAWQQVVIFASLLSIIVGALGAIGQNNIKRLMAYSSINNVGFILIGLATG
TKAGASAMLVYLAIYVAMSVAGFVAVLLLRDENGEQVEAISDLAGLSKTRPWLALALASVMFSLAGIPPLFGFWGKFVVF
QAAVQADMIVLAALGIAASVIGAFYYLKVVKVMYFDEAVDKVRGEGELSHAVLLALSTLAISPLGYLGSKCLGTLADYAA
SALFHVG

Sequences:

>Translated_487_residues
MDIARSLALTAPEVLISISGLVLLLIAAWGGDKASRLISILAVAVLAACAVLAAPALWGQAMGADVAAFGGQHHADAFAA
FAKLLIYASAGVTLIVAQSFFDKAKAMRAEFPLLVLFASLGMGMMVSAGDLLALYIGLELSSLASYVMAAFLRTDDRSAE
SGLKYFVLGSLASGILLFGMSLTYGFTGTTSFAGINAALSGGLSTGATFGVIFVLAGLAFKISAAPFHMWTPDVYEGAPT
PVTTFFATASKVSALGLLMRVSFEAFGSQPHAWQQVVIFASLLSIIVGALGAIGQNNIKRLMAYSSINNVGFILIGLATG
TKAGASAMLVYLAIYVAMSVAGFVAVLLLRDENGEQVEAISDLAGLSKTRPWLALALASVMFSLAGIPPLFGFWGKFVVF
QAAVQADMIVLAALGIAASVIGAFYYLKVVKVMYFDEAVDKVRGEGELSHAVLLALSTLAISPLGYLGSKCLGTLADYAA
SALFHVG
>Mature_487_residues
MDIARSLALTAPEVLISISGLVLLLIAAWGGDKASRLISILAVAVLAACAVLAAPALWGQAMGADVAAFGGQHHADAFAA
FAKLLIYASAGVTLIVAQSFFDKAKAMRAEFPLLVLFASLGMGMMVSAGDLLALYIGLELSSLASYVMAAFLRTDDRSAE
SGLKYFVLGSLASGILLFGMSLTYGFTGTTSFAGINAALSGGLSTGATFGVIFVLAGLAFKISAAPFHMWTPDVYEGAPT
PVTTFFATASKVSALGLLMRVSFEAFGSQPHAWQQVVIFASLLSIIVGALGAIGQNNIKRLMAYSSINNVGFILIGLATG
TKAGASAMLVYLAIYVAMSVAGFVAVLLLRDENGEQVEAISDLAGLSKTRPWLALALASVMFSLAGIPPLFGFWGKFVVF
QAAVQADMIVLAALGIAASVIGAFYYLKVVKVMYFDEAVDKVRGEGELSHAVLLALSTLAISPLGYLGSKCLGTLADYAA
SALFHVG

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family [H]

Homologues:

Organism=Homo sapiens, GI251831108, Length=351, Percent_Identity=22.5071225071225, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI145693160, Length=442, Percent_Identity=36.6515837104072, Blast_Score=216, Evalue=3e-57,
Organism=Escherichia coli, GI1788614, Length=359, Percent_Identity=25.0696378830084, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1788831, Length=304, Percent_Identity=25.3289473684211, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI1788827, Length=297, Percent_Identity=25.5892255892256, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1788829, Length=285, Percent_Identity=24.5614035087719, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1788613, Length=305, Percent_Identity=26.2295081967213, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI2367154, Length=267, Percent_Identity=26.2172284644195, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010096
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 50428; Mature: 50428

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIARSLALTAPEVLISISGLVLLLIAAWGGDKASRLISILAVAVLAACAVLAAPALWGQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AMGADVAAFGGQHHADAFAAFAKLLIYASAGVTLIVAQSFFDKAKAMRAEFPLLVLFASL
HHCCCHHHCCCCCHHHHHHHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHCCHHHHHHHHH
GMGMMVSAGDLLALYIGLELSSLASYVMAAFLRTDDRSAESGLKYFVLGSLASGILLFGM
CCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHHHHHHH
SLTYGFTGTTSFAGINAALSGGLSTGATFGVIFVLAGLAFKISAAPFHMWTPDVYEGAPT
HHHCCCCCCCCHHCHHHHHHCCCCCCHHHHHHHHHHHHHHEEECCCCCCCCCHHCCCCCC
PVTTFFATASKVSALGLLMRVSFEAFGSQPHAWQQVVIFASLLSIIVGALGAIGQNNIKR
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LMAYSSINNVGFILIGLATGTKAGASAMLVYLAIYVAMSVAGFVAVLLLRDENGEQVEAI
HHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHH
SDLAGLSKTRPWLALALASVMFSLAGIPPLFGFWGKFVVFQAAVQADMIVLAALGIAASV
HHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGAFYYLKVVKVMYFDEAVDKVRGEGELSHAVLLALSTLAISPLGYLGSKCLGTLADYAA
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
SALFHVG
HHHHCCC
>Mature Secondary Structure
MDIARSLALTAPEVLISISGLVLLLIAAWGGDKASRLISILAVAVLAACAVLAAPALWGQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AMGADVAAFGGQHHADAFAAFAKLLIYASAGVTLIVAQSFFDKAKAMRAEFPLLVLFASL
HHCCCHHHCCCCCHHHHHHHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHCCHHHHHHHHH
GMGMMVSAGDLLALYIGLELSSLASYVMAAFLRTDDRSAESGLKYFVLGSLASGILLFGM
CCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHHHHHHH
SLTYGFTGTTSFAGINAALSGGLSTGATFGVIFVLAGLAFKISAAPFHMWTPDVYEGAPT
HHHCCCCCCCCHHCHHHHHHCCCCCCHHHHHHHHHHHHHHEEECCCCCCCCCHHCCCCCC
PVTTFFATASKVSALGLLMRVSFEAFGSQPHAWQQVVIFASLLSIIVGALGAIGQNNIKR
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LMAYSSINNVGFILIGLATGTKAGASAMLVYLAIYVAMSVAGFVAVLLLRDENGEQVEAI
HHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHH
SDLAGLSKTRPWLALALASVMFSLAGIPPLFGFWGKFVVFQAAVQADMIVLAALGIAASV
HHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGAFYYLKVVKVMYFDEAVDKVRGEGELSHAVLLALSTLAISPLGYLGSKCLGTLADYAA
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
SALFHVG
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA