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The map label for this gene is birA [H]

Identifier: 87200300

GI number: 87200300

Start: 2427307

End: 2428038

Strand: Reverse

Name: birA [H]

Synonym: Saro_2286

Alternate gene names: 87200300

Gene position: 2428038-2427307 (Counterclockwise)

Preceding gene: 87200301

Following gene: 87200299

Centisome position: 68.17

GC content: 66.53

Gene sequence:

>732_bases
TTGATCGCAACCGTTGCTGAAATCCCTTCGACCAATGCCGCGCTTCTGGCGCGGCTTGGCGGGGGAGAACTGCTCAACGA
AGGCGATTGGCTGGTTGCCGACCGGCAGACCGCCGGGCGCGGGCGCGCGGGGCGTTCGTGGTCCGATGGCTTCGGCAATT
TCATGGGCTCCACCGTCGTGCAGTTGCGCGGCGCCGATCCGCTGCCGCAGACGCTGGCGCTGGTTACCGGTCTTGCCGCC
CACCAGGCGGTCATGGCCCTGGCACCGCGCCTTTCCGACCTGCGGCTCAAATGGCCCAACGACCTTCTGGTGGGCGAGGC
CAAGCTGGCGGGCATCCTGCTGGAACGCCATGCGAATGCGGTGGTCGTGGGGATCGGCGTCAACCTGGCACAGGCTCCCG
ACGTTCCCGACCGCAAGACGGCGTCGCTATCGGGCCTGGGGTACGCGATTGCGCGCGATGCCTTTGCCACGGAACTGGCT
GACAGGTTTGCCGATGCACTGGCGCGCTGGCATCTGGGGGAATGGCCCTTGCTGCGCGAACAATGGCTGGCGCGCGCCCT
GCCTACGGGGACGCTGGTGTCGGTCAAGGACCGCGATCAAGGCACGATTATCGGGGCCTTCGGGGGTATAGACGAGGATG
GCGTTGCCCTTATGCGCTTGGCGGACGGGTCGGTCCGTGCCATCCACGCGGGCGATATAGAAATGGTGGGCTCCCATGCT
TCTGGCGGTTGA

Upstream 100 bases:

>100_bases
TCGACGCTGGCGATTTCTCCGCTGGGGTACCTGGGCAGCAAGTGCCTGGGCACGCTGGCGGACTATGCGGCCTCCGCGCT
GTTCCACGTCGGATGATCCA

Downstream 100 bases:

>100_bases
CGTCGGCAATACCAACATCGTTTTCGCGCTGTTCGAGGGGCGCGAAATCAAGTCGCGCTGGCGCGTTGCCACCGATCCGC
GCCGCACGGCCGACGAGTAC

Product: biotin--acetyl-CoA-carboxylase ligase

Products: NA

Alternate protein names: Biotin--protein ligase [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MIATVAEIPSTNAALLARLGGGELLNEGDWLVADRQTAGRGRAGRSWSDGFGNFMGSTVVQLRGADPLPQTLALVTGLAA
HQAVMALAPRLSDLRLKWPNDLLVGEAKLAGILLERHANAVVVGIGVNLAQAPDVPDRKTASLSGLGYAIARDAFATELA
DRFADALARWHLGEWPLLREQWLARALPTGTLVSVKDRDQGTIIGAFGGIDEDGVALMRLADGSVRAIHAGDIEMVGSHA
SGG

Sequences:

>Translated_243_residues
MIATVAEIPSTNAALLARLGGGELLNEGDWLVADRQTAGRGRAGRSWSDGFGNFMGSTVVQLRGADPLPQTLALVTGLAA
HQAVMALAPRLSDLRLKWPNDLLVGEAKLAGILLERHANAVVVGIGVNLAQAPDVPDRKTASLSGLGYAIARDAFATELA
DRFADALARWHLGEWPLLREQWLARALPTGTLVSVKDRDQGTIIGAFGGIDEDGVALMRLADGSVRAIHAGDIEMVGSHA
SGG
>Mature_243_residues
MIATVAEIPSTNAALLARLGGGELLNEGDWLVADRQTAGRGRAGRSWSDGFGNFMGSTVVQLRGADPLPQTLALVTGLAA
HQAVMALAPRLSDLRLKWPNDLLVGEAKLAGILLERHANAVVVGIGVNLAQAPDVPDRKTASLSGLGYAIARDAFATELA
DRFADALARWHLGEWPLLREQWLARALPTGTLVSVKDRDQGTIIGAFGGIDEDGVALMRLADGSVRAIHAGDIEMVGSHA
SGG

Specific function: Activates biotin to form biotinyl-5'-adenylate and transfers the biotin moiety to biotin-accepting proteins [H]

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=250, Percent_Identity=31.2, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR003142
- InterPro:   IPR004143 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 25437; Mature: 25437

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIATVAEIPSTNAALLARLGGGELLNEGDWLVADRQTAGRGRAGRSWSDGFGNFMGSTVV
CCEECCCCCCCCHHEEEECCCCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHCCEE
QLRGADPLPQTLALVTGLAAHQAVMALAPRLSDLRLKWPNDLLVGEAKLAGILLERHANA
EECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCEEECHHHHHHHHHHCCCCE
VVVGIGVNLAQAPDVPDRKTASLSGLGYAIARDAFATELADRFADALARWHLGEWPLLRE
EEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
QWLARALPTGTLVSVKDRDQGTIIGAFGGIDEDGVALMRLADGSVRAIHAGDIEMVGSHA
HHHHHHCCCCCEEEEEECCCCEEEEECCCCCCCCEEEEEECCCCEEEEECCCEEEECCCC
SGG
CCC
>Mature Secondary Structure
MIATVAEIPSTNAALLARLGGGELLNEGDWLVADRQTAGRGRAGRSWSDGFGNFMGSTVV
CCEECCCCCCCCHHEEEECCCCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHCCEE
QLRGADPLPQTLALVTGLAAHQAVMALAPRLSDLRLKWPNDLLVGEAKLAGILLERHANA
EECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCEEECHHHHHHHHHHCCCCE
VVVGIGVNLAQAPDVPDRKTASLSGLGYAIARDAFATELADRFADALARWHLGEWPLLRE
EEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
QWLARALPTGTLVSVKDRDQGTIIGAFGGIDEDGVALMRLADGSVRAIHAGDIEMVGSHA
HHHHHHCCCCCEEEEEECCCCEEEEECCCCCCCCEEEEEECCCCEEEEECCCEEEECCCC
SGG
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8422400 [H]