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The map label for this gene is nuoM [H]

Identifier: 87200302

GI number: 87200302

Start: 2429506

End: 2431056

Strand: Reverse

Name: nuoM [H]

Synonym: Saro_2288

Alternate gene names: 87200302

Gene position: 2431056-2429506 (Counterclockwise)

Preceding gene: 87200303

Following gene: 87200301

Centisome position: 68.26

GC content: 62.86

Gene sequence:

>1551_bases
ATGAACGGTTTTCTTGGGACCGGCTTCCCGATCCTTTCGCTGATGCTGCTGGTGCCGCTCGCAGCGGCGGTGGCGTGCCT
TGTCGTCCCGCGCGAACAGGCGCGGACGGTAGCTCTCGTCGCGACGCTGGTGGACCTCGTCCTTGGCGCGGTGCTGTGGG
CGAACTACGACGTCGGCGGCGCGCAGTGGCAGTTTACCGAAAACGCCCCGCTCTTCGCAGGCTTTGCCTGGAAGCTGGGC
ATCGACGGCATCGCGCTATTGCTGATCGCGCTCACGGTGTTCCTGATGCCGATCTGCATCGGGGCAAGCTGGGTATCGAT
CGACAAGCGCCAGGGCGAATACTACGCGGCGTTCCTCCTGATGGAGACGCTGATGATCGGCGTCTTCGCGGCGCAGGACT
TGTTCCTGTTCTACATCATGTTCGAGGCCGGGCTTATTCCGATGTACCTCATCATCGGTGTCTGGGGTGGGGCGGATCGT
ATCTACGCCAGCTACAAGTTCTTCCTCTACACGCTGCTCGGCTCGGTCCTGATGCTGATCGCGATGTTCTGGATGGTGAA
CGAGGCCGGGACGACCGACATTCCGACGCTCATGGTCTACAACTTCCCGGTGAAGGCGCAGACCTGGCTGTGGCTCGCGT
TCTTCGCCAGCTTCGCGGTGAAGATGCCGATGTGGCCGGTACACACCTGGCTTCCCGACGCGCACGTGCAGGCACCGACC
GCCGGTTCGGTCATTCTTGCCGGCGTTCTGCTGAAGATGGGCGGCTATGGCTTCATCCGCTTCTCGCTGCCGATGTTCCC
CGAAGCCTCCGCGCAGTTCGCGCCGCTGGTGTTCGGGCTGTCGATGGCGGCGGTTGTCATCACTTCGCTGATCGCGCTCG
TGCAGCACGACATGAAGAAGCTGATCGCCTATTCGTCGGTTGCGCACATGGCGATCGTCACGGTCGGTCTCTTTGCCTTC
AACCGCCAGGGCCTCGAAGGCTCGATGATCGTGATGCTCAGCCACGGTCTGGTTTCGGGCGCGCTGTTCCTCAGCGTCGG
CGTGATCTACGATCGGCTGCATACCCGCGAAATCGATCGCTACGGCGGCCTGTCGATCAACATGCCCTATTACGCGGTCT
TCTTCCTGCTATTCACCATGGCGTCGATCGGCCTTCCGGGGACGAGCGGTTTCGTCGGCGAATTCCTGTCGCTGATCGGC
ATCTACCAGCTTTCGAGCTGGGTGGCGCTGGTCTGCACGACCGGGATCATCCTCGGCGCGGCCTACATGCTCTACCTCTA
CCGCCGGGTGGCTTATGGCGAGCAGAAGAACGCCGATGCCGCCGCCATGCCCGATCTCGACAAGCGTGAGTGGGCGATGA
TGGTGCCGCTGGCGGCCGTCGTCCTGTGGATGGGCGTCTATCCCGAAAGCTTCATCGCGCCGATGCGCAAGGACATCGCG
TCGCTCGACGCCCGTCTCGCGCAGGCGAAGCCCAAGGGGGACGCGCAGGTCGCAGTCGGGAAGGCCAAGCCTGCAACCGA
AGAAAATTCCGCGCATGAGGGGGCGCACTGA

Upstream 100 bases:

>100_bases
GCAGCCGCGCCGCCGCCAAGCTCCAGTCGGGGTATCTCTATAGCTATGCGCTGGTCATGCTCGTCGGACTTGTCGGCGCG
ATCAGCTGGGTGATCGCACA

Downstream 100 bases:

>100_bases
TGGATATCGCCCGTTCGCTCGCGCTCACCGCGCCCGAGGTTCTGATTTCGATCTCGGGGCTGGTGCTGCTGCTGATTGCC
GCCTGGGGCGGTGACAAGGC

Product: proton-translocating NADH-quinone oxidoreductase, chain M

Products: NA

Alternate protein names: NADH dehydrogenase I subunit M; NDH-1 subunit M [H]

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MNGFLGTGFPILSLMLLVPLAAAVACLVVPREQARTVALVATLVDLVLGAVLWANYDVGGAQWQFTENAPLFAGFAWKLG
IDGIALLLIALTVFLMPICIGASWVSIDKRQGEYYAAFLLMETLMIGVFAAQDLFLFYIMFEAGLIPMYLIIGVWGGADR
IYASYKFFLYTLLGSVLMLIAMFWMVNEAGTTDIPTLMVYNFPVKAQTWLWLAFFASFAVKMPMWPVHTWLPDAHVQAPT
AGSVILAGVLLKMGGYGFIRFSLPMFPEASAQFAPLVFGLSMAAVVITSLIALVQHDMKKLIAYSSVAHMAIVTVGLFAF
NRQGLEGSMIVMLSHGLVSGALFLSVGVIYDRLHTREIDRYGGLSINMPYYAVFFLLFTMASIGLPGTSGFVGEFLSLIG
IYQLSSWVALVCTTGIILGAAYMLYLYRRVAYGEQKNADAAAMPDLDKREWAMMVPLAAVVLWMGVYPESFIAPMRKDIA
SLDARLAQAKPKGDAQVAVGKAKPATEENSAHEGAH

Sequences:

>Translated_516_residues
MNGFLGTGFPILSLMLLVPLAAAVACLVVPREQARTVALVATLVDLVLGAVLWANYDVGGAQWQFTENAPLFAGFAWKLG
IDGIALLLIALTVFLMPICIGASWVSIDKRQGEYYAAFLLMETLMIGVFAAQDLFLFYIMFEAGLIPMYLIIGVWGGADR
IYASYKFFLYTLLGSVLMLIAMFWMVNEAGTTDIPTLMVYNFPVKAQTWLWLAFFASFAVKMPMWPVHTWLPDAHVQAPT
AGSVILAGVLLKMGGYGFIRFSLPMFPEASAQFAPLVFGLSMAAVVITSLIALVQHDMKKLIAYSSVAHMAIVTVGLFAF
NRQGLEGSMIVMLSHGLVSGALFLSVGVIYDRLHTREIDRYGGLSINMPYYAVFFLLFTMASIGLPGTSGFVGEFLSLIG
IYQLSSWVALVCTTGIILGAAYMLYLYRRVAYGEQKNADAAAMPDLDKREWAMMVPLAAVVLWMGVYPESFIAPMRKDIA
SLDARLAQAKPKGDAQVAVGKAKPATEENSAHEGAH
>Mature_516_residues
MNGFLGTGFPILSLMLLVPLAAAVACLVVPREQARTVALVATLVDLVLGAVLWANYDVGGAQWQFTENAPLFAGFAWKLG
IDGIALLLIALTVFLMPICIGASWVSIDKRQGEYYAAFLLMETLMIGVFAAQDLFLFYIMFEAGLIPMYLIIGVWGGADR
IYASYKFFLYTLLGSVLMLIAMFWMVNEAGTTDIPTLMVYNFPVKAQTWLWLAFFASFAVKMPMWPVHTWLPDAHVQAPT
AGSVILAGVLLKMGGYGFIRFSLPMFPEASAQFAPLVFGLSMAAVVITSLIALVQHDMKKLIAYSSVAHMAIVTVGLFAF
NRQGLEGSMIVMLSHGLVSGALFLSVGVIYDRLHTREIDRYGGLSINMPYYAVFFLLFTMASIGLPGTSGFVGEFLSLIG
IYQLSSWVALVCTTGIILGAAYMLYLYRRVAYGEQKNADAAAMPDLDKREWAMMVPLAAVVLWMGVYPESFIAPMRKDIA
SLDARLAQAKPKGDAQVAVGKAKPATEENSAHEGAH

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=359, Percent_Identity=34.5403899721448, Blast_Score=200, Evalue=3e-51,
Organism=Homo sapiens, GI251831117, Length=385, Percent_Identity=22.8571428571429, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1788613, Length=496, Percent_Identity=36.0887096774194, Blast_Score=296, Evalue=1e-81,
Organism=Escherichia coli, GI1788827, Length=298, Percent_Identity=30.5369127516779, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1788831, Length=309, Percent_Identity=28.1553398058252, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI2367154, Length=250, Percent_Identity=27.2, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI1788614, Length=374, Percent_Identity=26.4705882352941, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI145693160, Length=273, Percent_Identity=26.3736263736264, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1788829, Length=459, Percent_Identity=24.8366013071895, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918
- InterPro:   IPR000260 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01059 Oxidored_q5_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 56236; Mature: 56236

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGFLGTGFPILSLMLLVPLAAAVACLVVPREQARTVALVATLVDLVLGAVLWANYDVGG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
AQWQFTENAPLFAGFAWKLGIDGIALLLIALTVFLMPICIGASWVSIDKRQGEYYAAFLL
CEEEECCCCCEEECCHHHHCCHHHHHHHHHHHHHHHHHHHCCHHEEECCCCCHHHHHHHH
METLMIGVFAAQDLFLFYIMFEAGLIPMYLIIGVWGGADRIYASYKFFLYTLLGSVLMLI
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
AMFWMVNEAGTTDIPTLMVYNFPVKAQTWLWLAFFASFAVKMPMWPVHTWLPDAHVQAPT
HHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCC
AGSVILAGVLLKMGGYGFIRFSLPMFPEASAQFAPLVFGLSMAAVVITSLIALVQHDMKK
CHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIAYSSVAHMAIVTVGLFAFNRQGLEGSMIVMLSHGLVSGALFLSVGVIYDRLHTREIDR
HHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHH
YGGLSINMPYYAVFFLLFTMASIGLPGTSGFVGEFLSLIGIYQLSSWVALVCTTGIILGA
HCCEEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AYMLYLYRRVAYGEQKNADAAAMPDLDKREWAMMVPLAAVVLWMGVYPESFIAPMRKDIA
HHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
SLDARLAQAKPKGDAQVAVGKAKPATEENSAHEGAH
HHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNGFLGTGFPILSLMLLVPLAAAVACLVVPREQARTVALVATLVDLVLGAVLWANYDVGG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
AQWQFTENAPLFAGFAWKLGIDGIALLLIALTVFLMPICIGASWVSIDKRQGEYYAAFLL
CEEEECCCCCEEECCHHHHCCHHHHHHHHHHHHHHHHHHHCCHHEEECCCCCHHHHHHHH
METLMIGVFAAQDLFLFYIMFEAGLIPMYLIIGVWGGADRIYASYKFFLYTLLGSVLMLI
HHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
AMFWMVNEAGTTDIPTLMVYNFPVKAQTWLWLAFFASFAVKMPMWPVHTWLPDAHVQAPT
HHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCC
AGSVILAGVLLKMGGYGFIRFSLPMFPEASAQFAPLVFGLSMAAVVITSLIALVQHDMKK
CHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIAYSSVAHMAIVTVGLFAFNRQGLEGSMIVMLSHGLVSGALFLSVGVIYDRLHTREIDR
HHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHH
YGGLSINMPYYAVFFLLFTMASIGLPGTSGFVGEFLSLIGIYQLSSWVALVCTTGIILGA
HCCEEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AYMLYLYRRVAYGEQKNADAAAMPDLDKREWAMMVPLAAVVLWMGVYPESFIAPMRKDIA
HHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
SLDARLAQAKPKGDAQVAVGKAKPATEENSAHEGAH
HHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8566820 [H]