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The map label for this gene is grpE

Identifier: 87200075

GI number: 87200075

Start: 2199768

End: 2200328

Strand: Reverse

Name: grpE

Synonym: Saro_2059

Alternate gene names: 87200075

Gene position: 2200328-2199768 (Counterclockwise)

Preceding gene: 87200076

Following gene: 87200073

Centisome position: 61.78

GC content: 65.6

Gene sequence:

>561_bases
ATGAGCGATAACGACACGCGCCCCACCGACGCAGAAGTCGAAGCCGAGCTGAAGGGCGTGCCCGAGGACATGATTGACCG
GACGTCCGATAACGATGAACTGGCCAAGCTGCGCGAGGAGCTGGAAACGGCCAGGCAGGACGTGCTCTATGCCAAGGCCG
AGACGCAGAACGTGCGTCGCCGCATGGAGAAGGACGTCGCCGACGCCCGCGCCTATGCCGCGACCGGCTTTGCGCGCGAC
ATCCTGTCGGTGGCCGACAACCTTTCGCGTGCGCTCGAATCGATCCCGGCGGACCTGCGCGAGGACGACAAGTTCAAGAA
CCTTGTCGCCGGCCTCGAGGCCACGGGCCGCGAGATCGAGAAGGTGTTTTCCAGCCACGGCATCGTGCGCATCGCCGCGA
TGGGCCTGCCGCTCGACCCGCACCAGCACCAGGCGATGATCGAGATGCCTTCTGCCGATGCGGAGCCCGGCACGGTTATC
CAGGAGCTTCAGGCCGGTTACATGATCAAGGACCGCCTGCTGCGGCCCGCGATGGTGGCGGTGGCCAAGAAGCCGGACTG
A

Upstream 100 bases:

>100_bases
TCGGCCCAACCCGGTTGAATTATGCGCGCGTCGTCCCCATGGTGGACTTCACCGCCCAGACCCTGGGCAAATTCATCGGA
CAAGACGGATTTTCGAGAAA

Downstream 100 bases:

>100_bases
CCTGGTCTGCGCCGTCGGGTCTCCGTTCAGGCGGGGGCCGGGACGGCCTGTAATTGCGTTCCGTAACGCAACCCGGAGCC
CCCGCCTGCGCGGGGGCTTC

Product: GrpE protein

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 186; Mature: 185

Protein sequence:

>186_residues
MSDNDTRPTDAEVEAELKGVPEDMIDRTSDNDELAKLREELETARQDVLYAKAETQNVRRRMEKDVADARAYAATGFARD
ILSVADNLSRALESIPADLREDDKFKNLVAGLEATGREIEKVFSSHGIVRIAAMGLPLDPHQHQAMIEMPSADAEPGTVI
QELQAGYMIKDRLLRPAMVAVAKKPD

Sequences:

>Translated_186_residues
MSDNDTRPTDAEVEAELKGVPEDMIDRTSDNDELAKLREELETARQDVLYAKAETQNVRRRMEKDVADARAYAATGFARD
ILSVADNLSRALESIPADLREDDKFKNLVAGLEATGREIEKVFSSHGIVRIAAMGLPLDPHQHQAMIEMPSADAEPGTVI
QELQAGYMIKDRLLRPAMVAVAKKPD
>Mature_185_residues
SDNDTRPTDAEVEAELKGVPEDMIDRTSDNDELAKLREELETARQDVLYAKAETQNVRRRMEKDVADARAYAATGFARDI
LSVADNLSRALESIPADLREDDKFKNLVAGLEATGREIEKVFSSHGIVRIAAMGLPLDPHQHQAMIEMPSADAEPGTVIQ
ELQAGYMIKDRLLRPAMVAVAKKPD

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=154, Percent_Identity=38.3116883116883, Blast_Score=116, Evalue=1e-26,
Organism=Homo sapiens, GI40255109, Length=136, Percent_Identity=31.6176470588235, Blast_Score=80, Evalue=1e-15,
Organism=Escherichia coli, GI1788967, Length=149, Percent_Identity=39.5973154362416, Blast_Score=93, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17552458, Length=136, Percent_Identity=38.2352941176471, Blast_Score=90, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6324806, Length=178, Percent_Identity=31.4606741573034, Blast_Score=102, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24653432, Length=173, Percent_Identity=32.3699421965318, Blast_Score=101, Evalue=3e-22,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_NOVAD (Q2G6M5)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_497332.1
- ProteinModelPortal:   Q2G6M5
- SMR:   Q2G6M5
- STRING:   Q2G6M5
- GeneID:   3917706
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_2059
- NMPDR:   fig|48935.1.peg.2087
- eggNOG:   COG0576
- HOGENOM:   HBG732630
- OMA:   NVRRRME
- PhylomeDB:   Q2G6M5
- ProtClustDB:   CLSK835067
- BioCyc:   NARO279238:SARO_2059-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 20587; Mature: 20456

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDNDTRPTDAEVEAELKGVPEDMIDRTSDNDELAKLREELETARQDVLYAKAETQNVRR
CCCCCCCCCCCHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMEKDVADARAYAATGFARDILSVADNLSRALESIPADLREDDKFKNLVAGLEATGREIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
KVFSSHGIVRIAAMGLPLDPHQHQAMIEMPSADAEPGTVIQELQAGYMIKDRLLRPAMVA
HHHHHCCEEEEEECCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHH
VAKKPD
HCCCCC
>Mature Secondary Structure 
SDNDTRPTDAEVEAELKGVPEDMIDRTSDNDELAKLREELETARQDVLYAKAETQNVRR
CCCCCCCCCCHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMEKDVADARAYAATGFARDILSVADNLSRALESIPADLREDDKFKNLVAGLEATGREIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
KVFSSHGIVRIAAMGLPLDPHQHQAMIEMPSADAEPGTVIQELQAGYMIKDRLLRPAMVA
HHHHHCCEEEEEECCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHH
VAKKPD
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA