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The map label for this gene is thyA [H]

Identifier: 87199994

GI number: 87199994

Start: 2094575

End: 2095525

Strand: Reverse

Name: thyA [H]

Synonym: Saro_1977

Alternate gene names: 87199994

Gene position: 2095525-2094575 (Counterclockwise)

Preceding gene: 87199996

Following gene: 87199993

Centisome position: 58.84

GC content: 61.72

Gene sequence:

>951_bases
GTGGCCATGTCCATCCTGCGCGATTCCGGCCGCCATTACGAATGGCAATATCTCGATCTCATGCGCCGCATTTGGGAACA
TGGCGACGAGCGGGTAGATCGGACCGGCGTCGGTACGCGGTCGGTCTTCGGTGCTGAGCTCAGATTCGATCTTTCCGATG
GGCGGATGCCGTTGCTCACTACCAAACGCGTCTACTGGAAGACGGCAACGCGTGAGTTCCTATGGTTCCTGACCGGCAAT
ACCAATATCCGCCCCCTCTGCGCGCAGGGTGTGGAGATCTGGACCGATTGGCCGCTCGACCGTTACCGCAAGGAGACCGG
GGACGACATTTCGCGGAAGGACTTTTCGGCACGGATCGTCGCGGACGAGGCATTCGCGTTGCGGTGGGGCGACCTTGGAC
CGGTCTATGGCAAGCAATGGGTGGACTGGCCGGTGTTCGAGCCTGTGGGCGACGGCCTGTTCCGCCGACGCGAAGCCGGC
GTGAACCAGGTGGCGGACGTGGTTGATTCGCTGCGGCATAATCCCGGCAGCAGGCGTCACATCATCGAGGGTTGGAACGT
CGCCGAGATCGACCGGATGGCGCTGCCTCCCTGCCACAAGACATACCAGTTCCACGTCAGCGGCAATCGGCTGAACGGCT
TGCTCTATCAACGCAGTTGCGACGTCGCGCTGGGGCTTCCCTTCAACCTGTGGGGCGCCGCACTGCTCGTACGTCTGCTG
GCCCAGCAATGTGACCTTCAACCCGGCGAACTGGTTTGGATGGGTGGAGATACGCATCTCTACCTGAACCACGCGGATCT
CGTTGAAGCGCAACTTTCGCGGGAACCGGAGGGTGATCCGCGCCTTGCCATCCTGCGGCGACCGGATAGTATCTTCGGTT
ACCGGATCGAGGATTTCGAGGTGACCGGATATGCACCGCAGGGGCACCTTTCCGCGCCTGTTGCCGTCTGA

Upstream 100 bases:

>100_bases
GGGCGCCCTATACGAAGCAGTTTTCCAACTGGCAAGCGGCCTTTGGAAAAGTTTTTTTGCAGCCCGGTTTTTGCGCCTTG
GCCCAGCCCGGGATAGAAGC

Downstream 100 bases:

>100_bases
TGGCCTGATTGACTCGAAAAGGCGTGTGAAATAAAATAACACACGTCTATAACTTTGCGACTGTACGACTTTCGCATTTG
CGAAGGTTGATTCGGCGCGG

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MAMSILRDSGRHYEWQYLDLMRRIWEHGDERVDRTGVGTRSVFGAELRFDLSDGRMPLLTTKRVYWKTATREFLWFLTGN
TNIRPLCAQGVEIWTDWPLDRYRKETGDDISRKDFSARIVADEAFALRWGDLGPVYGKQWVDWPVFEPVGDGLFRRREAG
VNQVADVVDSLRHNPGSRRHIIEGWNVAEIDRMALPPCHKTYQFHVSGNRLNGLLYQRSCDVALGLPFNLWGAALLVRLL
AQQCDLQPGELVWMGGDTHLYLNHADLVEAQLSREPEGDPRLAILRRPDSIFGYRIEDFEVTGYAPQGHLSAPVAV

Sequences:

>Translated_316_residues
MAMSILRDSGRHYEWQYLDLMRRIWEHGDERVDRTGVGTRSVFGAELRFDLSDGRMPLLTTKRVYWKTATREFLWFLTGN
TNIRPLCAQGVEIWTDWPLDRYRKETGDDISRKDFSARIVADEAFALRWGDLGPVYGKQWVDWPVFEPVGDGLFRRREAG
VNQVADVVDSLRHNPGSRRHIIEGWNVAEIDRMALPPCHKTYQFHVSGNRLNGLLYQRSCDVALGLPFNLWGAALLVRLL
AQQCDLQPGELVWMGGDTHLYLNHADLVEAQLSREPEGDPRLAILRRPDSIFGYRIEDFEVTGYAPQGHLSAPVAV
>Mature_315_residues
AMSILRDSGRHYEWQYLDLMRRIWEHGDERVDRTGVGTRSVFGAELRFDLSDGRMPLLTTKRVYWKTATREFLWFLTGNT
NIRPLCAQGVEIWTDWPLDRYRKETGDDISRKDFSARIVADEAFALRWGDLGPVYGKQWVDWPVFEPVGDGLFRRREAGV
NQVADVVDSLRHNPGSRRHIIEGWNVAEIDRMALPPCHKTYQFHVSGNRLNGLLYQRSCDVALGLPFNLWGAALLVRLLA
QQCDLQPGELVWMGGDTHLYLNHADLVEAQLSREPEGDPRLAILRRPDSIFGYRIEDFEVTGYAPQGHLSAPVAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=311, Percent_Identity=40.5144694533762, Blast_Score=221, Evalue=6e-58,
Organism=Escherichia coli, GI1789191, Length=301, Percent_Identity=48.1727574750831, Blast_Score=282, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI71993377, Length=305, Percent_Identity=37.7049180327869, Blast_Score=204, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6324648, Length=318, Percent_Identity=33.3333333333333, Blast_Score=164, Evalue=2e-41,
Organism=Drosophila melanogaster, GI17137556, Length=305, Percent_Identity=41.3114754098361, Blast_Score=232, Evalue=3e-61,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 36250; Mature: 36118

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMSILRDSGRHYEWQYLDLMRRIWEHGDERVDRTGVGTRSVFGAELRFDLSDGRMPLLT
CCCHHHHCCCCCEEHHHHHHHHHHHHCCHHHHHHCCCCCHHEECEEEEEECCCCCEEEEE
TKRVYWKTATREFLWFLTGNTNIRPLCAQGVEIWTDWPLDRYRKETGDDISRKDFSARIV
CCCEEEEECCEEEEEEEECCCCCCHHHHCCCEEEECCCHHHHHHHCCCCCCCCCCCEEEE
ADEAFALRWGDLGPVYGKQWVDWPVFEPVGDGLFRRREAGVNQVADVVDSLRHNPGSRRH
ECCEEEEEECCCCCCCCCCCCCCCCCCHHCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCE
IIEGWNVAEIDRMALPPCHKTYQFHVSGNRLNGLLYQRSCDVALGLPFNLWGAALLVRLL
EECCCCCHHHHHHCCCCCCCCEEEEEECCCCCEEEEEECCCEEECCCHHHHHHHHHHHHH
AQQCDLQPGELVWMGGDTHLYLNHADLVEAQLSREPEGDPRLAILRRPDSIFGYRIEDFE
HHHHCCCCCCEEEECCCEEEEEECHHHHHHHHCCCCCCCCCEEEEECCCCHHCEEEEEEE
VTGYAPQGHLSAPVAV
EEECCCCCCCCCCCCC
>Mature Secondary Structure 
AMSILRDSGRHYEWQYLDLMRRIWEHGDERVDRTGVGTRSVFGAELRFDLSDGRMPLLT
CCHHHHCCCCCEEHHHHHHHHHHHHCCHHHHHHCCCCCHHEECEEEEEECCCCCEEEEE
TKRVYWKTATREFLWFLTGNTNIRPLCAQGVEIWTDWPLDRYRKETGDDISRKDFSARIV
CCCEEEEECCEEEEEEEECCCCCCHHHHCCCEEEECCCHHHHHHHCCCCCCCCCCCEEEE
ADEAFALRWGDLGPVYGKQWVDWPVFEPVGDGLFRRREAGVNQVADVVDSLRHNPGSRRH
ECCEEEEEECCCCCCCCCCCCCCCCCCHHCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCE
IIEGWNVAEIDRMALPPCHKTYQFHVSGNRLNGLLYQRSCDVALGLPFNLWGAALLVRLL
EECCCCCHHHHHHCCCCCCCCEEEEEECCCCCEEEEEECCCEEECCCHHHHHHHHHHHHH
AQQCDLQPGELVWMGGDTHLYLNHADLVEAQLSREPEGDPRLAILRRPDSIFGYRIEDFE
HHHHCCCCCCEEEECCCEEEEEECHHHHHHHHCCCCCCCCCEEEEECCCCHHCEEEEEEE
VTGYAPQGHLSAPVAV
EEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA