Click here to switch to the map view.

The map label for this gene is bkdA1 [H]

Identifier: 87199993

GI number: 87199993

Start: 2093149

End: 2094423

Strand: Reverse

Name: bkdA1 [H]

Synonym: Saro_1976

Alternate gene names: 87199993

Gene position: 2094423-2093149 (Counterclockwise)

Preceding gene: 87199994

Following gene: 87199992

Centisome position: 58.81

GC content: 64.08

Gene sequence:

>1275_bases
ATGGCGCGCGGCAATCTGCCGTCGCTATCGCTCCACGTGCCGGAACCGAAGTTCCGGCCGGGTGACAAGGTCGATTATTC
CGACCTTGCCATTTCGCGCGCGGGGGAACAGCCGCGACCCGACGAGCAGTGCGAGGCTTCCGAAACCCACCCGTTGTGCC
TCGATCTGGTGCGCGTGCTTGGCGATGACGACCGTGCGATCGGCCCTTGGGACCCCCGGTTGGACGCCGACACGCTGCGC
CGCATGCTGCGCACGATGGCGCTGACCCGTGCTTTCGACGACCGCATGTATCGCGGCCAGCGACAGGGCAAGACCAGCTT
CTACATGAAGTGCACGGGCGAAGAGGCGACATCGGTCGCCCCGGCCATGGCCTTGGCGGATGACGACATGGTCTTCCCCA
GCTACCGCCAGCAGGGCATCCTGATCGCGCGTGGCTATCCGTTGGTCGAGATGATCAACCAGATCTATTCCAATCGTGCC
GACAAGCTGAAGGGACGCCAGTTGCCGATCATGTATTCGGCGCGCGAGCAGTCGTTCTTCACGATCTCGGGCAACCTCGC
CACGCAGTACCCGCAGGCCGTGGGTTGGGCCATGGCAAGCGCGATCAAGGGCGACAGCCGCATCGCCGCGACCTGGATCG
GCGAAGGGTCCACGGCTGAGGGCGACTTCCATTCGGCCATGACTTTCGCAGCAGTCTACAATGCGCCCGTCATCTTCAAT
GTGGTGAACAACCAGTGGGCCATTTCCAGTTTTTCGGGTTTTGCCGGCGCGGAGAGGACGACTTTTGCCGCCCGCGCGAT
CGGCTATGGCATCGCCGGCTTGCGGGTGGACGGTAACGATCCGCTTGCTGTCTTCGCGGCAACCCAGTGGGCCGCGAACC
GCGCCCGCGCCAATGCCGGCCCTACGCTGATCGAGCACTTCACCTACCGTGCCGAGGGGCACTCGACTTCCGATGATCCC
ACCCAGTACCGTTCCGCGCAGGAGCGGGAGGAGTGGCCGCTGGGCGACCCGGTCAACCGGCTGAAGAAGCACCTCGTGGC
CCTGGGCGAGTGGTCGGACGAGCAGCACGAGGCGATGGACCGTGAACTCGTCGACCTGGTCAAGGCGGCCACGAAGGAGG
CCGAAAAGAACGGCATCCTGGGGCACGGGCTGCATCACCCGTTCCATACAATGTTCGAGGACGTCTTCGAGGAACTGCCC
TGGCATCTCCGCGAACAGAGCGAGCAGGCAATCCGCGAGCGTCGGATCAAGTGGCCGGAATGGAAAGAGTCATGA

Upstream 100 bases:

>100_bases
ACTTTGCGACTGTACGACTTTCGCATTTGCGAAGGTTGATTCGGCGCGGGAGAGTCCAGATGCAGGCGTTTGCCCTATGA
GTAGCGAGGGGATGAGCGGG

Downstream 100 bases:

>100_bases
CCTACGAAGAATCTCCGGTCAGCCTCGCCGAGGCCCCGACCCGTCGTCTGAACATGATCGAAGCGATCAATGACGCTCTC
GACATCATGATGGAGCGTGA

Product: branched-chain alpha-keto acid dehydrogenase E1 component

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain; BCKDH E1-alpha [H]

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MARGNLPSLSLHVPEPKFRPGDKVDYSDLAISRAGEQPRPDEQCEASETHPLCLDLVRVLGDDDRAIGPWDPRLDADTLR
RMLRTMALTRAFDDRMYRGQRQGKTSFYMKCTGEEATSVAPAMALADDDMVFPSYRQQGILIARGYPLVEMINQIYSNRA
DKLKGRQLPIMYSAREQSFFTISGNLATQYPQAVGWAMASAIKGDSRIAATWIGEGSTAEGDFHSAMTFAAVYNAPVIFN
VVNNQWAISSFSGFAGAERTTFAARAIGYGIAGLRVDGNDPLAVFAATQWAANRARANAGPTLIEHFTYRAEGHSTSDDP
TQYRSAQEREEWPLGDPVNRLKKHLVALGEWSDEQHEAMDRELVDLVKAATKEAEKNGILGHGLHHPFHTMFEDVFEELP
WHLREQSEQAIRERRIKWPEWKES

Sequences:

>Translated_424_residues
MARGNLPSLSLHVPEPKFRPGDKVDYSDLAISRAGEQPRPDEQCEASETHPLCLDLVRVLGDDDRAIGPWDPRLDADTLR
RMLRTMALTRAFDDRMYRGQRQGKTSFYMKCTGEEATSVAPAMALADDDMVFPSYRQQGILIARGYPLVEMINQIYSNRA
DKLKGRQLPIMYSAREQSFFTISGNLATQYPQAVGWAMASAIKGDSRIAATWIGEGSTAEGDFHSAMTFAAVYNAPVIFN
VVNNQWAISSFSGFAGAERTTFAARAIGYGIAGLRVDGNDPLAVFAATQWAANRARANAGPTLIEHFTYRAEGHSTSDDP
TQYRSAQEREEWPLGDPVNRLKKHLVALGEWSDEQHEAMDRELVDLVKAATKEAEKNGILGHGLHHPFHTMFEDVFEELP
WHLREQSEQAIRERRIKWPEWKES
>Mature_423_residues
ARGNLPSLSLHVPEPKFRPGDKVDYSDLAISRAGEQPRPDEQCEASETHPLCLDLVRVLGDDDRAIGPWDPRLDADTLRR
MLRTMALTRAFDDRMYRGQRQGKTSFYMKCTGEEATSVAPAMALADDDMVFPSYRQQGILIARGYPLVEMINQIYSNRAD
KLKGRQLPIMYSAREQSFFTISGNLATQYPQAVGWAMASAIKGDSRIAATWIGEGSTAEGDFHSAMTFAAVYNAPVIFNV
VNNQWAISSFSGFAGAERTTFAARAIGYGIAGLRVDGNDPLAVFAATQWAANRARANAGPTLIEHFTYRAEGHSTSDDPT
QYRSAQEREEWPLGDPVNRLKKHLVALGEWSDEQHEAMDRELVDLVKAATKEAEKNGILGHGLHHPFHTMFEDVFEELPW
HLREQSEQAIRERRIKWPEWKES

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCKDHA family [H]

Homologues:

Organism=Homo sapiens, GI11386135, Length=363, Percent_Identity=40.771349862259, Blast_Score=254, Evalue=1e-67,
Organism=Homo sapiens, GI258645172, Length=361, Percent_Identity=40.7202216066482, Blast_Score=254, Evalue=2e-67,
Organism=Homo sapiens, GI4885543, Length=301, Percent_Identity=30.2325581395349, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI291084742, Length=309, Percent_Identity=30.0970873786408, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI4505685, Length=309, Percent_Identity=30.0970873786408, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI291084744, Length=316, Percent_Identity=29.4303797468354, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI291084757, Length=309, Percent_Identity=27.5080906148867, Blast_Score=88, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI86563357, Length=343, Percent_Identity=40.8163265306122, Blast_Score=243, Evalue=1e-64,
Organism=Caenorhabditis elegans, GI86563355, Length=343, Percent_Identity=40.8163265306122, Blast_Score=243, Evalue=1e-64,
Organism=Caenorhabditis elegans, GI17536047, Length=330, Percent_Identity=27.2727272727273, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI32564172, Length=330, Percent_Identity=27.2727272727273, Blast_Score=122, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6321026, Length=330, Percent_Identity=28.4848484848485, Blast_Score=123, Evalue=5e-29,
Organism=Drosophila melanogaster, GI21355903, Length=357, Percent_Identity=37.5350140056022, Blast_Score=221, Evalue=7e-58,
Organism=Drosophila melanogaster, GI24639748, Length=337, Percent_Identity=29.9703264094956, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24639740, Length=306, Percent_Identity=28.1045751633987, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24639744, Length=306, Percent_Identity=28.1045751633987, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI28571106, Length=306, Percent_Identity=28.1045751633987, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24639746, Length=306, Percent_Identity=28.1045751633987, Blast_Score=105, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017
- InterPro:   IPR022593 [H]

Pfam domain/function: PF00676 E1_dh; PF12573 OxoDH_E1alpha_N [H]

EC number: =1.2.4.4 [H]

Molecular weight: Translated: 47533; Mature: 47402

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARGNLPSLSLHVPEPKFRPGDKVDYSDLAISRAGEQPRPDEQCEASETHPLCLDLVRVL
CCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHH
GDDDRAIGPWDPRLDADTLRRMLRTMALTRAFDDRMYRGQRQGKTSFYMKCTGEEATSVA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHH
PAMALADDDMVFPSYRQQGILIARGYPLVEMINQIYSNRADKLKGRQLPIMYSAREQSFF
HHHHCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCHHHHCCCCCCCEEEECCCCEEE
TISGNLATQYPQAVGWAMASAIKGDSRIAATWIGEGSTAEGDFHSAMTFAAVYNAPVIFN
EEECCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEE
VVNNQWAISSFSGFAGAERTTFAARAIGYGIAGLRVDGNDPLAVFAATQWAANRARANAG
ECCCCEEECCCCCCCCCCHHHHHHHHHHCCEEEEEECCCCCEEEEEEHHHHHHHHCCCCC
PTLIEHFTYRAEGHSTSDDPTQYRSAQEREEWPLGDPVNRLKKHLVALGEWSDEQHEAMD
CHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHH
RELVDLVKAATKEAEKNGILGHGLHHPFHTMFEDVFEELPWHLREQSEQAIRERRIKWPE
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCC
WKES
CCCC
>Mature Secondary Structure 
ARGNLPSLSLHVPEPKFRPGDKVDYSDLAISRAGEQPRPDEQCEASETHPLCLDLVRVL
CCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHH
GDDDRAIGPWDPRLDADTLRRMLRTMALTRAFDDRMYRGQRQGKTSFYMKCTGEEATSVA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHH
PAMALADDDMVFPSYRQQGILIARGYPLVEMINQIYSNRADKLKGRQLPIMYSAREQSFF
HHHHCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCHHHHCCCCCCCEEEECCCCEEE
TISGNLATQYPQAVGWAMASAIKGDSRIAATWIGEGSTAEGDFHSAMTFAAVYNAPVIFN
EEECCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEE
VVNNQWAISSFSGFAGAERTTFAARAIGYGIAGLRVDGNDPLAVFAATQWAANRARANAG
ECCCCEEECCCCCCCCCCHHHHHHHHHHCCEEEEEECCCCCEEEEEEHHHHHHHHCCCCC
PTLIEHFTYRAEGHSTSDDPTQYRSAQEREEWPLGDPVNRLKKHLVALGEWSDEQHEAMD
CHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHH
RELVDLVKAATKEAEKNGILGHGLHHPFHTMFEDVFEELPWHLREQSEQAIRERRIKWPE
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCC
WKES
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]