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The map label for this gene is ntrB [H]

Identifier: 87199944

GI number: 87199944

Start: 2038957

End: 2040078

Strand: Direct

Name: ntrB [H]

Synonym: Saro_1927

Alternate gene names: 87199944

Gene position: 2038957-2040078 (Clockwise)

Preceding gene: 87199943

Following gene: 87199945

Centisome position: 57.25

GC content: 63.81

Gene sequence:

>1122_bases
ATGATCGCGCTCCCTGACTGGCGTCGTCAGGAACCGGGGCAGAAGCCCGAACCGAAGCGCGTCGTCGCGAGCCTTCCGCT
TGCGCTGCTGCAGCTCGATCCCGACCTCATCGTGGCCGCGGTAAACCCGGCGGCCGAACAACTCATGGGGCAGGGCGCAC
GCCGGATCGTTGGAAAGTCGGTCGCAGAACTGTTCGAATTCGAGGAGCCGCTTATCCTCGGCCGCCTGGCTGAAGGTGAA
GCCCAACTTTTCGCGCGCGGAGTTGGCGTGCGCATCATGGGGCAGCCCGCGCGACGTTTTGACGTGATGACCAGCCCCGT
GACTCATTGCCCCGGCTGGCAGCTCCTGATGCTTCACGAAGGTGTGGGCGTCGAGGCCCTGTCTGGCGACGGTCGCGGCG
CAGGAGGCGGGGAGGGTGTTGCTTTGCGCGCACCCGAAGTCCTTGCCCACGAGATCAAGAACCCGCTGGCCGGCATAAAG
GGCGCGGCGCAGCTTCTTGATCGCAAGCTGTCCGAAAGCGATCGCGCGATGACCGGCCTGATCACCGCCGAGGTCGACCG
TATCGCCAAACTGATCGACCAGATGCAGTCGCTTTCCCGGCGGAGCGCCGAACCCGCGCAGCCGTGCAATCTGCACGAAG
CTGTCCGCCGGGCCGAAGCGGTGCTTGCAGCGGCCAGCCCGGAATCGGTCACGATCGTCGAGGAGTTCGACCCCTCGCTC
CCGCCGATCATGGCCAATCCGGATTCGCTCGTCCAGGTTCTGCTTAACCTGCTGAGCAATGCGCGCGAAGCCTGCCTCGC
CAATGAAGAGCCGCGCATCATCGTGCGCACGCGCTTTGCAAGCGGCATTCAGCTACATGCCGGCCCCGGTGGAAGGCCCC
TTCGCCTGCCTATCGAATTGCGCGTATCCGACAACGGACCGGGTATCGATCCCACATTGCGCGACCACATCTTCGAACCC
TTTGTCACCGCAAAGAAGAACGGCCAGGGCCTTGGTCTTGCCCTTGTCCAGAAGCTGGTGCGAGAGATGAATGGCCGCAT
TACCCATGATCGCGACGAGGTGGGTGGCTGGACCCATTTTCGCATCCATCTTCCTGTCGCCGGATCGGTTCCCACCGAAT
GA

Upstream 100 bases:

>100_bases
CAAAGGCCGTCCTGGCAAGTCTGGCGGATTTCTACGGACGAGCGATCGAACACCAGCACTCCGCCGCCGAGGCTTCCGCT
GCGGAGCGGCGCGCAGCCGC

Downstream 100 bases:

>100_bases
GCAAGCGTGTACTTCTCGTCGAGGACGATGCTTCGATTGCCCTGGTCATTACCGCGGCACTTGAAGCTGAAGGCTTCACT
GTCGATCGCTGCGATTCGAT

Product: signal transduction histidine kinase, nitrogen specific, NtrB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MIALPDWRRQEPGQKPEPKRVVASLPLALLQLDPDLIVAAVNPAAEQLMGQGARRIVGKSVAELFEFEEPLILGRLAEGE
AQLFARGVGVRIMGQPARRFDVMTSPVTHCPGWQLLMLHEGVGVEALSGDGRGAGGGEGVALRAPEVLAHEIKNPLAGIK
GAAQLLDRKLSESDRAMTGLITAEVDRIAKLIDQMQSLSRRSAEPAQPCNLHEAVRRAEAVLAAASPESVTIVEEFDPSL
PPIMANPDSLVQVLLNLLSNAREACLANEEPRIIVRTRFASGIQLHAGPGGRPLRLPIELRVSDNGPGIDPTLRDHIFEP
FVTAKKNGQGLGLALVQKLVREMNGRITHDRDEVGGWTHFRIHLPVAGSVPTE

Sequences:

>Translated_373_residues
MIALPDWRRQEPGQKPEPKRVVASLPLALLQLDPDLIVAAVNPAAEQLMGQGARRIVGKSVAELFEFEEPLILGRLAEGE
AQLFARGVGVRIMGQPARRFDVMTSPVTHCPGWQLLMLHEGVGVEALSGDGRGAGGGEGVALRAPEVLAHEIKNPLAGIK
GAAQLLDRKLSESDRAMTGLITAEVDRIAKLIDQMQSLSRRSAEPAQPCNLHEAVRRAEAVLAAASPESVTIVEEFDPSL
PPIMANPDSLVQVLLNLLSNAREACLANEEPRIIVRTRFASGIQLHAGPGGRPLRLPIELRVSDNGPGIDPTLRDHIFEP
FVTAKKNGQGLGLALVQKLVREMNGRITHDRDEVGGWTHFRIHLPVAGSVPTE
>Mature_373_residues
MIALPDWRRQEPGQKPEPKRVVASLPLALLQLDPDLIVAAVNPAAEQLMGQGARRIVGKSVAELFEFEEPLILGRLAEGE
AQLFARGVGVRIMGQPARRFDVMTSPVTHCPGWQLLMLHEGVGVEALSGDGRGAGGGEGVALRAPEVLAHEIKNPLAGIK
GAAQLLDRKLSESDRAMTGLITAEVDRIAKLIDQMQSLSRRSAEPAQPCNLHEAVRRAEAVLAAASPESVTIVEEFDPSL
PPIMANPDSLVQVLLNLLSNAREACLANEEPRIIVRTRFASGIQLHAGPGGRPLRLPIELRVSDNGPGIDPTLRDHIFEP
FVTAKKNGQGLGLALVQKLVREMNGRITHDRDEVGGWTHFRIHLPVAGSVPTE

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=370, Percent_Identity=30.8108108108108, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1788549, Length=321, Percent_Identity=29.9065420560748, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1790436, Length=222, Percent_Identity=31.5315315315315, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1789808, Length=224, Percent_Identity=27.6785714285714, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1787894, Length=238, Percent_Identity=26.0504201680672, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87081816, Length=234, Percent_Identity=29.9145299145299, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 40206; Mature: 40206

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIALPDWRRQEPGQKPEPKRVVASLPLALLQLDPDLIVAAVNPAAEQLMGQGARRIVGKS
CCCCCCCCCCCCCCCCCHHHHHHHCCHHHEECCCCEEEEECCHHHHHHHHHHHHHHHHHH
VAELFEFEEPLILGRLAEGEAQLFARGVGVRIMGQPARRFDVMTSPVTHCPGWQLLMLHE
HHHHHHCCCCCEEEEECCCHHHHHHHCCCEEEECCCHHHHHHHCCCCCCCCCCEEEEEEC
GVGVEALSGDGRGAGGGEGVALRAPEVLAHEIKNPLAGIKGAAQLLDRKLSESDRAMTGL
CCCEEEECCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITAEVDRIAKLIDQMQSLSRRSAEPAQPCNLHEAVRRAEAVLAAASPESVTIVEEFDPSL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHEECCCCCEEEEECCCCCC
PPIMANPDSLVQVLLNLLSNAREACLANEEPRIIVRTRFASGIQLHAGPGGRPLRLPIEL
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEECCCCCEEEEEEEE
RVSDNGPGIDPTLRDHIFEPFVTAKKNGQGLGLALVQKLVREMNGRITHDRDEVGGWTHF
EECCCCCCCCCHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHCCCEEEE
RIHLPVAGSVPTE
EEEEEECCCCCCC
>Mature Secondary Structure
MIALPDWRRQEPGQKPEPKRVVASLPLALLQLDPDLIVAAVNPAAEQLMGQGARRIVGKS
CCCCCCCCCCCCCCCCCHHHHHHHCCHHHEECCCCEEEEECCHHHHHHHHHHHHHHHHHH
VAELFEFEEPLILGRLAEGEAQLFARGVGVRIMGQPARRFDVMTSPVTHCPGWQLLMLHE
HHHHHHCCCCCEEEEECCCHHHHHHHCCCEEEECCCHHHHHHHCCCCCCCCCCEEEEEEC
GVGVEALSGDGRGAGGGEGVALRAPEVLAHEIKNPLAGIKGAAQLLDRKLSESDRAMTGL
CCCEEEECCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITAEVDRIAKLIDQMQSLSRRSAEPAQPCNLHEAVRRAEAVLAAASPESVTIVEEFDPSL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHEECCCCCEEEEECCCCCC
PPIMANPDSLVQVLLNLLSNAREACLANEEPRIIVRTRFASGIQLHAGPGGRPLRLPIEL
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEECCCCCEEEEEEEE
RVSDNGPGIDPTLRDHIFEPFVTAKKNGQGLGLALVQKLVREMNGRITHDRDEVGGWTHF
EECCCCCCCCCHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHCCCEEEE
RIHLPVAGSVPTE
EEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2710108; 8355615 [H]