Click here to switch to the map view.

The map label for this gene is ntrC [H]

Identifier: 87199945

GI number: 87199945

Start: 2040075

End: 2041508

Strand: Direct

Name: ntrC [H]

Synonym: Saro_1928

Alternate gene names: 87199945

Gene position: 2040075-2041508 (Clockwise)

Preceding gene: 87199944

Following gene: 87199946

Centisome position: 57.28

GC content: 60.95

Gene sequence:

>1434_bases
ATGAGCAAGCGTGTACTTCTCGTCGAGGACGATGCTTCGATTGCCCTGGTCATTACCGCGGCACTTGAAGCTGAAGGCTT
CACTGTCGATCGCTGCGATTCGATCGCTGGACGGGACCGTCTGCTTTCAGCGCAGACCTACGACCTCCTGCTGACCGATG
TCATGCTGACTGACGGTGACGGCATCGAAACGCTGGGCCCTGTGCGCGAGGCGCATCCCACGCTTCCGATCATCATCCTT
TCAGCACAGAACACCCTCGATACAGCCGTCAGGGCGAGCGACACAGGCGCATTCGAATACTTTCCCAAGCCCTTCGATCT
GGAAGAACTGGTCCGCACCGTAACCCAGGCCATCGGCAATGCCGGCGGGGTTGGTGCCGAACTGCCACAGGATGTGCCGC
AGGGCCTCCCGCTCGTTGGGCGAAGTTCCGCCATGCAGGCCGTGTATCGGATGATCACGAGGGTTCTGCGCAATGATCTG
ACAGTGCTGATTCTCGGAGAGTCCGGCACCGGCAAGGAGCTCGTGGCAGAGGCAATTCACCAGCTCGGCAACCGCCGGTC
GGGGCCTTTCGTCGCAGTGAATACCGCCGCCATTCCCGCAGAACTGATCGAAAGTGAGCTGTTCGGGCATGAAAAAGGCG
CCTTCACCGGTGCCGTAGCGCGATCCATCGGCAAGTTCGAACAGGCCAGCGGCGGGACCCTGTTCCTCGACGAAATCGGC
GACATGCCCATGCAGGCCCAGACCCGTTTGCTGCGGGCTTTGCAATCAGGCCGGATTCGACGGGTTGGCGGGCGTGAGGA
AATCATCCTCGACTGCCGCATCGTTGCCGCGACAAACCGCGATCTCCTGCCGATGATCGCGGCGGGGACATTCCGCGAGG
ACCTCTACTACCGCCTCGCCGTCGTACCGATTGAACTGCCCCCGCTGCGGGAACGGGCAGATGATATTCCAGCGTTATCG
CAGCATTTCCTCGCCAAGGCAGCCCTCGAAGGTCTGCCACGACGCCAACTTACACAAGCGGGCGCGGACCTTCTGTCCCG
CCAGCCCTGGCGAGGCAACGTCCGCGAACTGCGCAATTTCGTATACCGCCTTGCACTGCTGGCACGTGACGAAGTGATCG
ATGCCTCGACCATCGAGCCACTTCTGGCGCAAGAAGCCACGGGGGCGGCGCGTTCATCCGAATCGGACGAAAGGCGACCA
TCCGATCTTGCCTCTGCAGTGGCCGCGTGGCTGTCGGCGCAGAACCTCCAGCCGGGCGAGGTCTATGATGCAGCGCTTGC
CGCATTTGAACGACCTCTGTTCCTCCAGATCCTTGCGCTGACTGGCGGGAACCAGCTTCGTGCCGCCCAAATACTTGGTA
TCAATAGAAATACTCTGCGCAAACGGCTTTCCGACCTGAATATCACACCCGACGAGTTCGCCAGTCGCGATTAG

Upstream 100 bases:

>100_bases
TGCGAGAGATGAATGGCCGCATTACCCATGATCGCGACGAGGTGGGTGGCTGGACCCATTTTCGCATCCATCTTCCTGTC
GCCGGATCGGTTCCCACCGA

Downstream 100 bases:

>100_bases
CCAGAGCTGCCGGTGAGAAGCGGCCTAACGACGCTCGACGGTTTCCTCTTGCATTTTTGCAACAGGGGTGTTGTGAAGTC
GCAACGATGGAGCGCACACC

Product: two component sigma-54 specific Fis family transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MSKRVLLVEDDASIALVITAALEAEGFTVDRCDSIAGRDRLLSAQTYDLLLTDVMLTDGDGIETLGPVREAHPTLPIIIL
SAQNTLDTAVRASDTGAFEYFPKPFDLEELVRTVTQAIGNAGGVGAELPQDVPQGLPLVGRSSAMQAVYRMITRVLRNDL
TVLILGESGTGKELVAEAIHQLGNRRSGPFVAVNTAAIPAELIESELFGHEKGAFTGAVARSIGKFEQASGGTLFLDEIG
DMPMQAQTRLLRALQSGRIRRVGGREEIILDCRIVAATNRDLLPMIAAGTFREDLYYRLAVVPIELPPLRERADDIPALS
QHFLAKAALEGLPRRQLTQAGADLLSRQPWRGNVRELRNFVYRLALLARDEVIDASTIEPLLAQEATGAARSSESDERRP
SDLASAVAAWLSAQNLQPGEVYDAALAAFERPLFLQILALTGGNQLRAAQILGINRNTLRKRLSDLNITPDEFASRD

Sequences:

>Translated_477_residues
MSKRVLLVEDDASIALVITAALEAEGFTVDRCDSIAGRDRLLSAQTYDLLLTDVMLTDGDGIETLGPVREAHPTLPIIIL
SAQNTLDTAVRASDTGAFEYFPKPFDLEELVRTVTQAIGNAGGVGAELPQDVPQGLPLVGRSSAMQAVYRMITRVLRNDL
TVLILGESGTGKELVAEAIHQLGNRRSGPFVAVNTAAIPAELIESELFGHEKGAFTGAVARSIGKFEQASGGTLFLDEIG
DMPMQAQTRLLRALQSGRIRRVGGREEIILDCRIVAATNRDLLPMIAAGTFREDLYYRLAVVPIELPPLRERADDIPALS
QHFLAKAALEGLPRRQLTQAGADLLSRQPWRGNVRELRNFVYRLALLARDEVIDASTIEPLLAQEATGAARSSESDERRP
SDLASAVAAWLSAQNLQPGEVYDAALAAFERPLFLQILALTGGNQLRAAQILGINRNTLRKRLSDLNITPDEFASRD
>Mature_476_residues
SKRVLLVEDDASIALVITAALEAEGFTVDRCDSIAGRDRLLSAQTYDLLLTDVMLTDGDGIETLGPVREAHPTLPIIILS
AQNTLDTAVRASDTGAFEYFPKPFDLEELVRTVTQAIGNAGGVGAELPQDVPQGLPLVGRSSAMQAVYRMITRVLRNDLT
VLILGESGTGKELVAEAIHQLGNRRSGPFVAVNTAAIPAELIESELFGHEKGAFTGAVARSIGKFEQASGGTLFLDEIGD
MPMQAQTRLLRALQSGRIRRVGGREEIILDCRIVAATNRDLLPMIAAGTFREDLYYRLAVVPIELPPLRERADDIPALSQ
HFLAKAALEGLPRRQLTQAGADLLSRQPWRGNVRELRNFVYRLALLARDEVIDASTIEPLLAQEATGAARSSESDERRPS
DLASAVAAWLSAQNLQPGEVYDAALAAFERPLFLQILALTGGNQLRAAQILGINRNTLRKRLSDLNITPDEFASRD

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=472, Percent_Identity=43.6440677966102, Blast_Score=348, Evalue=4e-97,
Organism=Escherichia coli, GI1790437, Length=463, Percent_Identity=41.6846652267819, Blast_Score=320, Evalue=1e-88,
Organism=Escherichia coli, GI1788550, Length=480, Percent_Identity=37.2916666666667, Blast_Score=288, Evalue=8e-79,
Organism=Escherichia coli, GI1788905, Length=471, Percent_Identity=36.0934182590234, Blast_Score=281, Evalue=6e-77,
Organism=Escherichia coli, GI87082117, Length=350, Percent_Identity=42.5714285714286, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI1789087, Length=340, Percent_Identity=43.5294117647059, Blast_Score=239, Evalue=4e-64,
Organism=Escherichia coli, GI1789233, Length=266, Percent_Identity=46.2406015037594, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI87082152, Length=255, Percent_Identity=47.4509803921569, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI87081872, Length=335, Percent_Identity=38.8059701492537, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1786524, Length=245, Percent_Identity=46.530612244898, Blast_Score=198, Evalue=7e-52,
Organism=Escherichia coli, GI1787583, Length=235, Percent_Identity=42.9787234042553, Blast_Score=185, Evalue=5e-48,
Organism=Escherichia coli, GI87081858, Length=461, Percent_Identity=26.2472885032538, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1789828, Length=243, Percent_Identity=37.4485596707819, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1790552, Length=122, Percent_Identity=34.4262295081967, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1786784, Length=114, Percent_Identity=34.2105263157895, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI87082012, Length=113, Percent_Identity=36.283185840708, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1786599, Length=113, Percent_Identity=33.6283185840708, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 51745; Mature: 51614

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4 ; PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRVLLVEDDASIALVITAALEAEGFTVDRCDSIAGRDRLLSAQTYDLLLTDVMLTDGD
CCCCEEEEECCCCEEEEEEEECCCCCCCHHHHHHHCCHHHHHCHHHHHHHHHHHEEECCC
GIETLGPVREAHPTLPIIILSAQNTLDTAVRASDTGAFEYFPKPFDLEELVRTVTQAIGN
CCCHHCCHHHCCCCCEEEEEECCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
AGGVGAELPQDVPQGLPLVGRSSAMQAVYRMITRVLRNDLTVLILGESGTGKELVAEAIH
CCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHH
QLGNRRSGPFVAVNTAAIPAELIESELFGHEKGAFTGAVARSIGKFEQASGGTLFLDEIG
HHCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHC
DMPMQAQTRLLRALQSGRIRRVGGREEIILDCRIVAATNRDLLPMIAAGTFREDLYYRLA
CCCHHHHHHHHHHHHCCCEEECCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHEEEEE
VVPIELPPLRERADDIPALSQHFLAKAALEGLPRRQLTQAGADLLSRQPWRGNVRELRNF
EEEECCCCHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHH
VYRLALLARDEVIDASTIEPLLAQEATGAARSSESDERRPSDLASAVAAWLSAQNLQPGE
HHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCH
VYDAALAAFERPLFLQILALTGGNQLRAAQILGINRNTLRKRLSDLNITPDEFASRD
HHHHHHHHHCCCHHEEEEECCCCCCEEEEEEECCCHHHHHHHHHHCCCCHHHHCCCC
>Mature Secondary Structure 
SKRVLLVEDDASIALVITAALEAEGFTVDRCDSIAGRDRLLSAQTYDLLLTDVMLTDGD
CCCEEEEECCCCEEEEEEEECCCCCCCHHHHHHHCCHHHHHCHHHHHHHHHHHEEECCC
GIETLGPVREAHPTLPIIILSAQNTLDTAVRASDTGAFEYFPKPFDLEELVRTVTQAIGN
CCCHHCCHHHCCCCCEEEEEECCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
AGGVGAELPQDVPQGLPLVGRSSAMQAVYRMITRVLRNDLTVLILGESGTGKELVAEAIH
CCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHH
QLGNRRSGPFVAVNTAAIPAELIESELFGHEKGAFTGAVARSIGKFEQASGGTLFLDEIG
HHCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHC
DMPMQAQTRLLRALQSGRIRRVGGREEIILDCRIVAATNRDLLPMIAAGTFREDLYYRLA
CCCHHHHHHHHHHHHCCCEEECCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHEEEEE
VVPIELPPLRERADDIPALSQHFLAKAALEGLPRRQLTQAGADLLSRQPWRGNVRELRNF
EEEECCCCHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHH
VYRLALLARDEVIDASTIEPLLAQEATGAARSSESDERRPSDLASAVAAWLSAQNLQPGE
HHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCH
VYDAALAAFERPLFLQILALTGGNQLRAAQILGINRNTLRKRLSDLNITPDEFASRD
HHHHHHHHHCCCHHEEEEECCCCCCEEEEEEECCCHHHHHHHHHHCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1661370 [H]