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The map label for this gene is dus [H]

Identifier: 87199943

GI number: 87199943

Start: 2037905

End: 2038960

Strand: Direct

Name: dus [H]

Synonym: Saro_1926

Alternate gene names: 87199943

Gene position: 2037905-2038960 (Clockwise)

Preceding gene: 87199939

Following gene: 87199944

Centisome position: 57.22

GC content: 63.83

Gene sequence:

>1056_bases
ATGTCCCAGGTTCCCGTTCCGCCCCGCCTCAAGCCCATCCAGGTTGGCCCCGTCACCGTGAGCTGCCCGGTCGTGCTCGC
GCCAATGACGGGCGTTTCGGACCTGCCATTCCGCACGATCGTGCGCCGCTTCGGCTCGGGGCTCAACGTGACCGAGATGA
TTGCGAGCCCTGCGGCCATCCGTGAGACGCGGCAATCCATCCAGAAGGCCGCATGGCACCCGACGGAAGAACCGGTCTCC
ATGCAACTCGTTGGCTGCGAGCCCGAGCAGATGGCCGAAGCTGCCAAGCTTTCGGAAGACAAAGGCGCGGCAATTATCGA
CATCAACATGGGCTGCCCCGTGCGCAAGGTCGTTAACGGGGATGCCGGCTCGGCCTTGATGCGCGACATTCCGTTAGCTA
CCCGCCTGATCGAGGCCACGGTGAAGGCGGTGAAGGTGCCCGTCACCGTAAAGATGCGCATGGGCTGGTGCCACGACAGC
CTGAATGCTCCCGAACTCGCGCGCATTGCCGAGGATCTCGGCGCGAAGATGATTACCGTGCACGGACGCACCCGCAACCA
GATGTACAAGGGCAGTGCCGACTGGGCCTTCGTCCGCAGCGTCAAGGAGGCGGTGTCAATTCCAGTGATCGTCAACGGCG
ACATCTGCGGGATCGAGGATGTCGCTACCGCCATAGAGCAGAGCGGCGCGGACGGCGTGATGATCGGGCGGGGGTCCTAT
GGTCGTCCGTGGCTTCTCGGCCAGATCATGCATTGGCTCGACACCGGCGAGCGTCTGGCGGACCCCCCCCTTGCCGAACA
GTACGCCCTCATTGTCGAGCACTACCACGCGATGCAGGAGCATTACGGCGAGATGACCGGCGTCAACATGGCGCGCAAGC
ATCTCGGCTGGTATACCAAGGGGCTGCACGGCTCAGCCGATTTCCGCAACAAGGTCAACTTCATCGACGACCCAAAGGCC
GTCCTGGCAAGTCTGGCGGATTTCTACGGACGAGCGATCGAACACCAGCACTCCGCCGCCGAGGCTTCCGCTGCGGAGCG
GCGCGCAGCCGCATGA

Upstream 100 bases:

>100_bases
GGCCGCGCCTCTACCAGCGCTTGCCCCGCGCGGGCAACAGCCTTGCCCTTCCGGCCACAATCGACTAAGCGCTGCCTGTT
TTTTAGGCAGATTGCTGACC

Downstream 100 bases:

>100_bases
TCGCGCTCCCTGACTGGCGTCGTCAGGAACCGGGGCAGAAGCCCGAACCGAAGCGCGTCGTCGCGAGCCTTCCGCTTGCG
CTGCTGCAGCTCGATCCCGA

Product: dihydrouridine synthase TIM-barrel protein nifR3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MSQVPVPPRLKPIQVGPVTVSCPVVLAPMTGVSDLPFRTIVRRFGSGLNVTEMIASPAAIRETRQSIQKAAWHPTEEPVS
MQLVGCEPEQMAEAAKLSEDKGAAIIDINMGCPVRKVVNGDAGSALMRDIPLATRLIEATVKAVKVPVTVKMRMGWCHDS
LNAPELARIAEDLGAKMITVHGRTRNQMYKGSADWAFVRSVKEAVSIPVIVNGDICGIEDVATAIEQSGADGVMIGRGSY
GRPWLLGQIMHWLDTGERLADPPLAEQYALIVEHYHAMQEHYGEMTGVNMARKHLGWYTKGLHGSADFRNKVNFIDDPKA
VLASLADFYGRAIEHQHSAAEASAAERRAAA

Sequences:

>Translated_351_residues
MSQVPVPPRLKPIQVGPVTVSCPVVLAPMTGVSDLPFRTIVRRFGSGLNVTEMIASPAAIRETRQSIQKAAWHPTEEPVS
MQLVGCEPEQMAEAAKLSEDKGAAIIDINMGCPVRKVVNGDAGSALMRDIPLATRLIEATVKAVKVPVTVKMRMGWCHDS
LNAPELARIAEDLGAKMITVHGRTRNQMYKGSADWAFVRSVKEAVSIPVIVNGDICGIEDVATAIEQSGADGVMIGRGSY
GRPWLLGQIMHWLDTGERLADPPLAEQYALIVEHYHAMQEHYGEMTGVNMARKHLGWYTKGLHGSADFRNKVNFIDDPKA
VLASLADFYGRAIEHQHSAAEASAAERRAAA
>Mature_350_residues
SQVPVPPRLKPIQVGPVTVSCPVVLAPMTGVSDLPFRTIVRRFGSGLNVTEMIASPAAIRETRQSIQKAAWHPTEEPVSM
QLVGCEPEQMAEAAKLSEDKGAAIIDINMGCPVRKVVNGDAGSALMRDIPLATRLIEATVKAVKVPVTVKMRMGWCHDSL
NAPELARIAEDLGAKMITVHGRTRNQMYKGSADWAFVRSVKEAVSIPVIVNGDICGIEDVATAIEQSGADGVMIGRGSYG
RPWLLGQIMHWLDTGERLADPPLAEQYALIVEHYHAMQEHYGEMTGVNMARKHLGWYTKGLHGSADFRNKVNFIDDPKAV
LASLADFYGRAIEHQHSAAEASAAERRAAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788462, Length=293, Percent_Identity=31.3993174061433, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI239788483, Length=280, Percent_Identity=33.2142857142857, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI40807366, Length=261, Percent_Identity=32.183908045977, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI31742496, Length=268, Percent_Identity=29.1044776119403, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI8923374, Length=279, Percent_Identity=26.1648745519713, Blast_Score=82, Evalue=6e-16,
Organism=Escherichia coli, GI1789660, Length=318, Percent_Identity=39.622641509434, Blast_Score=250, Evalue=8e-68,
Organism=Escherichia coli, GI1788462, Length=230, Percent_Identity=32.1739130434783, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI145693211, Length=310, Percent_Identity=26.7741935483871, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17543114, Length=294, Percent_Identity=31.6326530612245, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17507177, Length=235, Percent_Identity=31.063829787234, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI25144369, Length=222, Percent_Identity=31.0810810810811, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17510279, Length=204, Percent_Identity=28.921568627451, Blast_Score=68, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6323560, Length=229, Percent_Identity=34.4978165938865, Blast_Score=120, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6323433, Length=315, Percent_Identity=24.1269841269841, Blast_Score=99, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6323437, Length=233, Percent_Identity=29.6137339055794, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24585320, Length=281, Percent_Identity=32.7402135231317, Blast_Score=145, Evalue=5e-35,
Organism=Drosophila melanogaster, GI24580595, Length=226, Percent_Identity=32.7433628318584, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI19920448, Length=226, Percent_Identity=32.7433628318584, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI19921524, Length=268, Percent_Identity=28.3582089552239, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI45549423, Length=234, Percent_Identity=26.9230769230769, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 38091; Mature: 37960

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQVPVPPRLKPIQVGPVTVSCPVVLAPMTGVSDLPFRTIVRRFGSGLNVTEMIASPAAI
CCCCCCCCCCCCEEECCEEEECCEEEECCCCCCCCCHHHHHHHHCCCCCHHHHHHCCHHH
RETRQSIQKAAWHPTEEPVSMQLVGCEPEQMAEAAKLSEDKGAAIIDINMGCPVRKVVNG
HHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHCC
DAGSALMRDIPLATRLIEATVKAVKVPVTVKMRMGWCHDSLNAPELARIAEDLGAKMITV
CCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCEEEEE
HGRTRNQMYKGSADWAFVRSVKEAVSIPVIVNGDICGIEDVATAIEQSGADGVMIGRGSY
ECCCCCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHCCCCEEEECCCCC
GRPWLLGQIMHWLDTGERLADPPLAEQYALIVEHYHAMQEHYGEMTGVNMARKHLGWYTK
CCCHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLHGSADFRNKVNFIDDPKAVLASLADFYGRAIEHQHSAAEASAAERRAAA
CCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SQVPVPPRLKPIQVGPVTVSCPVVLAPMTGVSDLPFRTIVRRFGSGLNVTEMIASPAAI
CCCCCCCCCCCEEECCEEEECCEEEECCCCCCCCCHHHHHHHHCCCCCHHHHHHCCHHH
RETRQSIQKAAWHPTEEPVSMQLVGCEPEQMAEAAKLSEDKGAAIIDINMGCPVRKVVNG
HHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHCC
DAGSALMRDIPLATRLIEATVKAVKVPVTVKMRMGWCHDSLNAPELARIAEDLGAKMITV
CCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCEEEEE
HGRTRNQMYKGSADWAFVRSVKEAVSIPVIVNGDICGIEDVATAIEQSGADGVMIGRGSY
ECCCCCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHCCCCEEEECCCCC
GRPWLLGQIMHWLDTGERLADPPLAEQYALIVEHYHAMQEHYGEMTGVNMARKHLGWYTK
CCCHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLHGSADFRNKVNFIDDPKAVLASLADFYGRAIEHQHSAAEASAAERRAAA
CCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7553451 [H]