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The map label for this gene is smeIM [H]

Identifier: 87199857

GI number: 87199857

Start: 1939534

End: 1940676

Strand: Direct

Name: smeIM [H]

Synonym: Saro_1840

Alternate gene names: 87199857

Gene position: 1939534-1940676 (Clockwise)

Preceding gene: 87199856

Following gene: 87199858

Centisome position: 54.46

GC content: 63.08

Gene sequence:

>1143_bases
ATGGCAGTTGCAACCAAGGAACGCGCTAAGGCTCTTCGCGCGGCACCGGCAAAGGTGCTGAAGGCAGACATCGCGCTGCC
CGTGAACCAGATCCTGCGCGGCGATTGCATTGCCGAGATGCGCAAGCTGCCCGACGCCTCCATCGACATGATCTTCGCCG
ATCCGCCCTACAACCTCCAGCTTGGCGGCGATCTGGCTCGTCCCGATGGCAGCCATGTGGACGCCGTCACCAACGATTGG
GACAAGTTCTCGAGCTTTGCCGCCTACGACAAGTTCACGCGCGAATGGCTGGTCGAGGCGCGCCGCCTGCTGAAGCCGGA
TGGTTCGATCTGGGTGATCGGCAGCTACCACAACATCTTCCGCGTGGGTGCGCTGCTGCAGGATCTGGGGTTCTGGATTC
TCAACGACATCATCTGGCGCAAGGCCAACCCGATGCCCAATTTCAAGGGCACCCGCTTCACCAACGCGCACGAAACGCTG
ATCTGGGCGTCGAAGAGCGAGAAGTCGAAGTACACCTTCAACTATCGCGCGATGAAGACCCTGAACGACGAATTGCAGAT
GCGCTCCGACTGGGTTCTGCCGATCTGTTCGGGGCCGGAGCGCCTGCGCCGCAACGGCACCAAGGCGCACCCGACGCAGA
AGCCAGAGGCGCTGCTCTATCGCGTGATGCTTGCGACGACCAACAAGGGCGACGTCGTGCTGGACCCGTTTTTCGGCACT
GGCACCACCGGCGCGGTGGCCAAGCGGCTTGGCCGCAACTGGATCGGCTGCGAACGCGAGGATGACTACATCGAGGTCGC
CAACGAGCGCATCGAACTGGCGCTGCCGCTTGACGAAAGCGCGCTGACGACGATGCAGTCGAAGCGTAGCGCGCCCAAGG
TGGCGTTCGGCGCACTGGTCGAAAGCGGTTATCTGGCTCCCGGCACGCGGCTCACGGCCAAGAAGGGGCGGTTCAATGCG
GTCGTTCGCGCCGACGGGTCGCTTCAGTCCGAAGCCGAGATCGGTTCGATCCACGGGCTCGGGGCAAAGCTCCAGGGTGC
GCCTTCGTGCAATGGCTGGACGTTCTGGCATGTCGAGCACGAAGGCGAGGTGAAGCCGATCGACGCTCTGCGCCAGCTCT
ACCTGCTCGCCGTGGAAGATTGA

Upstream 100 bases:

>100_bases
CGCTGGCTGCGACTCGCCGAAATCTGGCGAGTCGCAGCTTGACGGCGAGTCCGCGATGACTCAAACGGTGGGCTTATCCA
CAAGGGGTCAGGGACTAGAT

Downstream 100 bases:

>100_bases
TCCTCTTGCCAGCGTACTGCGCCCCCGCCTAGGCGGGGGCAGGGGCAAAGGGCCCTGGCGGGGACTGGAAATGCTGAAAG
TCTACATTCGTCCGATCGCG

Product: DNA methylase N-4/N-6

Products: NA

Alternate protein names: M.SmeI; Adenine-specific methyltransferase SmeIP; M.CcrMI ortholog; Modification methylase SmeIP; M.SmeIP [H]

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MAVATKERAKALRAAPAKVLKADIALPVNQILRGDCIAEMRKLPDASIDMIFADPPYNLQLGGDLARPDGSHVDAVTNDW
DKFSSFAAYDKFTREWLVEARRLLKPDGSIWVIGSYHNIFRVGALLQDLGFWILNDIIWRKANPMPNFKGTRFTNAHETL
IWASKSEKSKYTFNYRAMKTLNDELQMRSDWVLPICSGPERLRRNGTKAHPTQKPEALLYRVMLATTNKGDVVLDPFFGT
GTTGAVAKRLGRNWIGCEREDDYIEVANERIELALPLDESALTTMQSKRSAPKVAFGALVESGYLAPGTRLTAKKGRFNA
VVRADGSLQSEAEIGSIHGLGAKLQGAPSCNGWTFWHVEHEGEVKPIDALRQLYLLAVED

Sequences:

>Translated_380_residues
MAVATKERAKALRAAPAKVLKADIALPVNQILRGDCIAEMRKLPDASIDMIFADPPYNLQLGGDLARPDGSHVDAVTNDW
DKFSSFAAYDKFTREWLVEARRLLKPDGSIWVIGSYHNIFRVGALLQDLGFWILNDIIWRKANPMPNFKGTRFTNAHETL
IWASKSEKSKYTFNYRAMKTLNDELQMRSDWVLPICSGPERLRRNGTKAHPTQKPEALLYRVMLATTNKGDVVLDPFFGT
GTTGAVAKRLGRNWIGCEREDDYIEVANERIELALPLDESALTTMQSKRSAPKVAFGALVESGYLAPGTRLTAKKGRFNA
VVRADGSLQSEAEIGSIHGLGAKLQGAPSCNGWTFWHVEHEGEVKPIDALRQLYLLAVED
>Mature_379_residues
AVATKERAKALRAAPAKVLKADIALPVNQILRGDCIAEMRKLPDASIDMIFADPPYNLQLGGDLARPDGSHVDAVTNDWD
KFSSFAAYDKFTREWLVEARRLLKPDGSIWVIGSYHNIFRVGALLQDLGFWILNDIIWRKANPMPNFKGTRFTNAHETLI
WASKSEKSKYTFNYRAMKTLNDELQMRSDWVLPICSGPERLRRNGTKAHPTQKPEALLYRVMLATTNKGDVVLDPFFGTG
TTGAVAKRLGRNWIGCEREDDYIEVANERIELALPLDESALTTMQSKRSAPKVAFGALVESGYLAPGTRLTAKKGRFNAV
VRADGSLQSEAEIGSIHGLGAKLQGAPSCNGWTFWHVEHEGEVKPIDALRQLYLLAVED

Specific function: This methylase recognizes the double-stranded sequence GANTC and causes specific methylation on A-2 on both strands. Ccrm-mediated methylation has important cellular functions. Appears to contribute to the accurate cell-cycle control of DNA replication an

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=281, Percent_Identity=30.2491103202847, Blast_Score=122, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 42300; Mature: 42169

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVATKERAKALRAAPAKVLKADIALPVNQILRGDCIAEMRKLPDASIDMIFADPPYNLQ
CCCCCHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEE
LGGDLARPDGSHVDAVTNDWDKFSSFAAYDKFTREWLVEARRLLKPDGSIWVIGSYHNIF
ECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHH
RVGALLQDLGFWILNDIIWRKANPMPNFKGTRFTNAHETLIWASKSEKSKYTFNYRAMKT
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCEEEEECCCCCCEEEEEEHHHHH
LNDELQMRSDWVLPICSGPERLRRNGTKAHPTQKPEALLYRVMLATTNKGDVVLDPFFGT
HHHHHHHCCCCEEEECCCHHHHHHCCCCCCCCCCHHHHHHHHHHEECCCCCEEECCCCCC
GTTGAVAKRLGRNWIGCEREDDYIEVANERIELALPLDESALTTMQSKRSAPKVAFGALV
CCHHHHHHHHCCCCCCCCCCCCHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCHHHHHHH
ESGYLAPGTRLTAKKGRFNAVVRADGSLQSEAEIGSIHGLGAKLQGAPSCNGWTFWHVEH
HCCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCCEECCCCCCCCCCCCCCEEEEEEEC
EGEVKPIDALRQLYLLAVED
CCCCCHHHHHHHHHEEEECC
>Mature Secondary Structure 
AVATKERAKALRAAPAKVLKADIALPVNQILRGDCIAEMRKLPDASIDMIFADPPYNLQ
CCCCHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEE
LGGDLARPDGSHVDAVTNDWDKFSSFAAYDKFTREWLVEARRLLKPDGSIWVIGSYHNIF
ECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHH
RVGALLQDLGFWILNDIIWRKANPMPNFKGTRFTNAHETLIWASKSEKSKYTFNYRAMKT
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCEEEEECCCCCCEEEEEEHHHHH
LNDELQMRSDWVLPICSGPERLRRNGTKAHPTQKPEALLYRVMLATTNKGDVVLDPFFGT
HHHHHHHCCCCEEEECCCHHHHHHCCCCCCCCCCHHHHHHHHHHEECCCCCEEECCCCCC
GTTGAVAKRLGRNWIGCEREDDYIEVANERIELALPLDESALTTMQSKRSAPKVAFGALV
CCHHHHHHHHCCCCCCCCCCCCHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCHHHHHHH
ESGYLAPGTRLTAKKGRFNAVVRADGSLQSEAEIGSIHGLGAKLQGAPSCNGWTFWHVEH
HCCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCCEECCCCCCCCCCCCCCEEEEEEEC
EGEVKPIDALRQLYLLAVED
CCCCCHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9294447; 11481430 [H]