Click here to switch to the map view.

The map label for this gene is thiC

Identifier: 87199841

GI number: 87199841

Start: 1926312

End: 1928192

Strand: Direct

Name: thiC

Synonym: Saro_1824

Alternate gene names: 87199841

Gene position: 1926312-1928192 (Clockwise)

Preceding gene: 87199836

Following gene: 87199842

Centisome position: 54.09

GC content: 63.85

Gene sequence:

>1881_bases
ATGGCCGACATCAACTCCAAGCTGGAAATCGGCGTCACCACCGGGCCGATCCGCGGCAGCCGCAAGATCCACGTGGAAAG
CGCACGCTTTCCCGGCTTGACGGTGGCGATGCGCGAAATCCAGCTCGAGCCTTCGAGCGGCGAGCCGCCGGTACGCGTCT
ACGATACGTCCGGTCCGTACACCGACCCCAAAGTGACCATCGACATCGCGGCGGGCCTGCCCACTCTGCGTCGCGACTGG
ATCATGGCGCGCGGCGACGTGGAAGAATACGACGCGCGCGAAGTGAAGCCCGAGGACAATGGCCTCAAGGGTCCGGATCG
TTCTGCCGGGGTGCCGCCGTTCCCCAACGTCGTGAAGCGCCCTTTGCGTGCAAAGGCGGGCCAGAACGTCAGCCAGATGC
ACTATGCCCGCCGCGGGATCATCACGCCCGAGATGGAATACGTGGCGATCCGCGAGAACCTTGGCCGCAAGCAGGCCAAG
GAAGCGATGATCCGTGATGGGCAGGACTGGGGTGCGTCGATCCCCGATTACGTGACGCCCGAATTCGTGCGCGACGAAGT
GGCGCGTGGGCGGGCGATCATCCCGTCGAACATCAACCACCCTGAAAGCGAACCGATGGCGATCGGCCGCAACTTCCTGG
TGAAGATCAACGCCAACATCGGCAATTCGGCTGTGGCATCCGACGTCGCGAGCGAAGTCGACAAGATGGTCTGGTCGATC
CGCTGGGGTGCGGATACCGTGATGGACCTTTCGACGGGGCGGAACATCCACGACACCCGCGAATGGATCCTGCGCAATTC
GCCGGTGCCGATCGGCACGGTGCCGATCTACCAGGCGCTCGAAAAAGTCGGCGGCGTGGCCGAGGACCTGACCTGGGAAG
TGTTCCGCGACACGCTGATCGAACAGGCCGAGCAGGGCGTGGACTACTTCACCATCCATGCCGGCGTGCGCCTTCCCTAC
ATCCCGCTTGCCGCCAAGCGCATGACCGGGATCGTCAGCCGTGGCGGTTCGATCATGGCCAAGTGGTGCCTTGCGCATCA
CAAGGAGTCGTTCCTTTACGAGAACTTCGACGAGATCACCGAGATCATGAAGGCCTATGACGTGGCCTATTCGCTGGGCG
ATGGCCTGCGTCCCGGATCGATCTACGACGCCAACGACGAAGCACAGTTCGCCGAACTCTACACGCTGGGCGAACTGACC
AAGCGTGCCTGGGAACAGGACGTGCAGGTGATGATCGAGGGCCCCGGCCACGTGCCGATGCACAAGATCAAGGAGAACAT
GACGAAGCAGCTCGAGGCGTGCGGCGAAGCGCCGTTCTACACGCTCGGGCCGCTCGTCACCGACATCGCGCCGGGATACG
ACCACATCACCAGCGGCATCGGTGCGGCACAGATCGGCTGGTACGGCACGGCGATGCTCTGTTACGTCACGCCCAAGGAG
CATCTCGGCCTGCCGGATCGCGATGACGTGAAGGTCGGCGTGGTGACCTACAAGCTGGCAGCCCACGCCGCCGACCTCGC
CAAGGGCCACCCTGCGGCACAGGCGCGCGATGACGCGCTGAGCAAGGCGCGCTTCGAGTTCCGCTGGCGCGACCAGTTCA
ACCTGTCGCTCGATCCGGAAACCGCCGAGCAGTACCACGACCAGACGCTTCCGGCGGAAGGCGCAAAGACCGCGCACTTC
TGCTCGATGTGCGGTCCGAAGTTCTGCTCGATGAAGATCAGCCAGGAAGTTCGCGACTTTGCCGCCAAGCAGAATGCCGG
CATCGAGACGTTTGTCGCCAACGAGGCCGAAGCCGAGGCCGGCATGAAGGCGATGAGCGACAAGTACGACGAGATGGGCC
GCGAACTGTACATCGGCGCGGGCGGGCGCGAGCACGACTGA

Upstream 100 bases:

>100_bases
TTGAACCTGAACCCGTTAGCACGGGCGGAGGGAAGGGCAGTGCCTCGGCAAAGAACCCGGGCACGCGCGCTCCCCGACCG
CCTGAAAGAGGAGCGCTTGA

Downstream 100 bases:

>100_bases
GGCGGCGCTGCCGCCAGACGATCGGTCAGGACTGTCCCCTTGTCCGGGGACAGTCTTGCCGTCATCCTTTGCGAAAGATC
GCAGGGAGAGCGGCGGATGA

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 626; Mature: 625

Protein sequence:

>626_residues
MADINSKLEIGVTTGPIRGSRKIHVESARFPGLTVAMREIQLEPSSGEPPVRVYDTSGPYTDPKVTIDIAAGLPTLRRDW
IMARGDVEEYDAREVKPEDNGLKGPDRSAGVPPFPNVVKRPLRAKAGQNVSQMHYARRGIITPEMEYVAIRENLGRKQAK
EAMIRDGQDWGASIPDYVTPEFVRDEVARGRAIIPSNINHPESEPMAIGRNFLVKINANIGNSAVASDVASEVDKMVWSI
RWGADTVMDLSTGRNIHDTREWILRNSPVPIGTVPIYQALEKVGGVAEDLTWEVFRDTLIEQAEQGVDYFTIHAGVRLPY
IPLAAKRMTGIVSRGGSIMAKWCLAHHKESFLYENFDEITEIMKAYDVAYSLGDGLRPGSIYDANDEAQFAELYTLGELT
KRAWEQDVQVMIEGPGHVPMHKIKENMTKQLEACGEAPFYTLGPLVTDIAPGYDHITSGIGAAQIGWYGTAMLCYVTPKE
HLGLPDRDDVKVGVVTYKLAAHAADLAKGHPAAQARDDALSKARFEFRWRDQFNLSLDPETAEQYHDQTLPAEGAKTAHF
CSMCGPKFCSMKISQEVRDFAAKQNAGIETFVANEAEAEAGMKAMSDKYDEMGRELYIGAGGREHD

Sequences:

>Translated_626_residues
MADINSKLEIGVTTGPIRGSRKIHVESARFPGLTVAMREIQLEPSSGEPPVRVYDTSGPYTDPKVTIDIAAGLPTLRRDW
IMARGDVEEYDAREVKPEDNGLKGPDRSAGVPPFPNVVKRPLRAKAGQNVSQMHYARRGIITPEMEYVAIRENLGRKQAK
EAMIRDGQDWGASIPDYVTPEFVRDEVARGRAIIPSNINHPESEPMAIGRNFLVKINANIGNSAVASDVASEVDKMVWSI
RWGADTVMDLSTGRNIHDTREWILRNSPVPIGTVPIYQALEKVGGVAEDLTWEVFRDTLIEQAEQGVDYFTIHAGVRLPY
IPLAAKRMTGIVSRGGSIMAKWCLAHHKESFLYENFDEITEIMKAYDVAYSLGDGLRPGSIYDANDEAQFAELYTLGELT
KRAWEQDVQVMIEGPGHVPMHKIKENMTKQLEACGEAPFYTLGPLVTDIAPGYDHITSGIGAAQIGWYGTAMLCYVTPKE
HLGLPDRDDVKVGVVTYKLAAHAADLAKGHPAAQARDDALSKARFEFRWRDQFNLSLDPETAEQYHDQTLPAEGAKTAHF
CSMCGPKFCSMKISQEVRDFAAKQNAGIETFVANEAEAEAGMKAMSDKYDEMGRELYIGAGGREHD
>Mature_625_residues
ADINSKLEIGVTTGPIRGSRKIHVESARFPGLTVAMREIQLEPSSGEPPVRVYDTSGPYTDPKVTIDIAAGLPTLRRDWI
MARGDVEEYDAREVKPEDNGLKGPDRSAGVPPFPNVVKRPLRAKAGQNVSQMHYARRGIITPEMEYVAIRENLGRKQAKE
AMIRDGQDWGASIPDYVTPEFVRDEVARGRAIIPSNINHPESEPMAIGRNFLVKINANIGNSAVASDVASEVDKMVWSIR
WGADTVMDLSTGRNIHDTREWILRNSPVPIGTVPIYQALEKVGGVAEDLTWEVFRDTLIEQAEQGVDYFTIHAGVRLPYI
PLAAKRMTGIVSRGGSIMAKWCLAHHKESFLYENFDEITEIMKAYDVAYSLGDGLRPGSIYDANDEAQFAELYTLGELTK
RAWEQDVQVMIEGPGHVPMHKIKENMTKQLEACGEAPFYTLGPLVTDIAPGYDHITSGIGAAQIGWYGTAMLCYVTPKEH
LGLPDRDDVKVGVVTYKLAAHAADLAKGHPAAQARDDALSKARFEFRWRDQFNLSLDPETAEQYHDQTLPAEGAKTAHFC
SMCGPKFCSMKISQEVRDFAAKQNAGIETFVANEAEAEAGMKAMSDKYDEMGRELYIGAGGREHD

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=618, Percent_Identity=66.9902912621359, Blast_Score=846, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_NOVAD (Q2G7A9)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_497098.1
- STRING:   Q2G7A9
- GeneID:   3918384
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_1824
- eggNOG:   COG0422
- HOGENOM:   HBG285234
- OMA:   MADINSK
- PhylomeDB:   Q2G7A9
- ProtClustDB:   PRK09284
- BioCyc:   NARO279238:SARO_1824-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 69213; Mature: 69082

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: BINDING 219-219 BINDING 248-248 BINDING 277-277 BINDING 313-313 BINDING 413-413 BINDING 440-440

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADINSKLEIGVTTGPIRGSRKIHVESARFPGLTVAMREIQLEPSSGEPPVRVYDTSGPY
CCCCCCCEEEEEEECCCCCCCEEEEECCCCCCCEEEEEEEEECCCCCCCCEEEEECCCCC
TDPKVTIDIAAGLPTLRRDWIMARGDVEEYDAREVKPEDNGLKGPDRSAGVPPFPNVVKR
CCCEEEEEEECCCCHHHHHHEEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
PLRAKAGQNVSQMHYARRGIITPEMEYVAIRENLGRKQAKEAMIRDGQDWGASIPDYVTP
HHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCHHHCCCCCCCCCH
EFVRDEVARGRAIIPSNINHPESEPMAIGRNFLVKINANIGNSAVASDVASEVDKMVWSI
HHHHHHHHCCCEECCCCCCCCCCCCCEECCEEEEEEECCCCCHHHHHHHHHHHHHHHEEE
RWGADTVMDLSTGRNIHDTREWILRNSPVPIGTVPIYQALEKVGGVAEDLTWEVFRDTLI
ECCCCCEEECCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHH
EQAEQGVDYFTIHAGVRLPYIPLAAKRMTGIVSRGGSIMAKWCLAHHKESFLYENFDEIT
HHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHH
EIMKAYDVAYSLGDGLRPGSIYDANDEAQFAELYTLGELTKRAWEQDVQVMIEGPGHVPM
HHHHHHHHHHHHCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCH
HKIKENMTKQLEACGEAPFYTLGPLVTDIAPGYDHITSGIGAAQIGWYGTAMLCYVTPKE
HHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHCCCCHHHHCCCCEEEEEEECCHH
HLGLPDRDDVKVGVVTYKLAAHAADLAKGHPAAQARDDALSKARFEFRWRDQFNLSLDPE
HCCCCCCCCEEEEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEEEECCCCCEEECCH
TAEQYHDQTLPAEGAKTAHFCSMCGPKFCSMKISQEVRDFAAKQNAGIETFVANEAEAEA
HHHHHHCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHH
GMKAMSDKYDEMGRELYIGAGGREHD
HHHHHHHHHHHCCCEEEEECCCCCCC
>Mature Secondary Structure 
ADINSKLEIGVTTGPIRGSRKIHVESARFPGLTVAMREIQLEPSSGEPPVRVYDTSGPY
CCCCCCEEEEEEECCCCCCCEEEEECCCCCCCEEEEEEEEECCCCCCCCEEEEECCCCC
TDPKVTIDIAAGLPTLRRDWIMARGDVEEYDAREVKPEDNGLKGPDRSAGVPPFPNVVKR
CCCEEEEEEECCCCHHHHHHEEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
PLRAKAGQNVSQMHYARRGIITPEMEYVAIRENLGRKQAKEAMIRDGQDWGASIPDYVTP
HHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCHHHCCCCCCCCCH
EFVRDEVARGRAIIPSNINHPESEPMAIGRNFLVKINANIGNSAVASDVASEVDKMVWSI
HHHHHHHHCCCEECCCCCCCCCCCCCEECCEEEEEEECCCCCHHHHHHHHHHHHHHHEEE
RWGADTVMDLSTGRNIHDTREWILRNSPVPIGTVPIYQALEKVGGVAEDLTWEVFRDTLI
ECCCCCEEECCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHH
EQAEQGVDYFTIHAGVRLPYIPLAAKRMTGIVSRGGSIMAKWCLAHHKESFLYENFDEIT
HHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHH
EIMKAYDVAYSLGDGLRPGSIYDANDEAQFAELYTLGELTKRAWEQDVQVMIEGPGHVPM
HHHHHHHHHHHHCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCH
HKIKENMTKQLEACGEAPFYTLGPLVTDIAPGYDHITSGIGAAQIGWYGTAMLCYVTPKE
HHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHCCCCHHHHCCCCEEEEEEECCHH
HLGLPDRDDVKVGVVTYKLAAHAADLAKGHPAAQARDDALSKARFEFRWRDQFNLSLDPE
HCCCCCCCCEEEEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEEEECCCCCEEECCH
TAEQYHDQTLPAEGAKTAHFCSMCGPKFCSMKISQEVRDFAAKQNAGIETFVANEAEAEA
HHHHHHCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHH
GMKAMSDKYDEMGRELYIGAGGREHD
HHHHHHHHHHHCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA