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The map label for this gene is fixL [H]

Identifier: 87199632

GI number: 87199632

Start: 1684254

End: 1686080

Strand: Direct

Name: fixL [H]

Synonym: Saro_1615

Alternate gene names: 87199632

Gene position: 1684254-1686080 (Clockwise)

Preceding gene: 87199631

Following gene: 87199633

Centisome position: 47.29

GC content: 64.04

Gene sequence:

>1827_bases
ATGCCTGTCGCCGTGGCGGTATTCGACCGCGAAATGCGCTACATCGCCTGCAGCCAGCTTTGGCTTAGCGAACATCGCAT
GGCCGAGGCCGATGTCGTCGGGCGCCTGCACTACGATGTGTTTCCCGACATATCGGATACCTGGCGTGCAATTCATCAGC
GCGCGCTTGCCGGCGAGACGCTTTCCAGCAGGCTGGATCGCTACGAGCGTGCGGACGGATCGTCTGACTGGGTCCAATGG
AACATCCAGCCCTGGCACACCGGAAAGGGCGACATCGGCGGGGTCGTGATGTTCGCCCGCGTCGTCACCAACGAGATCGA
AGCCGATCACCAGAAGCACCTCCTCGGTCGGGAACTGGACATGCTGATCGACGGCGCCGTCGATTATGCGATCTTCATGC
TCGACGAAGCGGGGCGGGTCTCGCTGTGGAACCGAGGGGCGGAACGTCTCACCGGATGGTGCCAGGCCGACATCGCGCAA
AGCCACTACGAAAAGCTCTTCGTACAGGCCGATATCGATGCCGCGCTTCCGATCCTGCAGCTCAGGATGGCGGAGATCGT
AAACAGCTACCGCGGGCGCCATTTCATCGCGCGCCGCGACAGCACCGTGATCCTCGCCGACGTCATCCTCACCCCGATCC
GCAGCGAGGGCAGGCTCACCGGGTTCGGCGTCGTGCTTCGCGACGTGACGGGTGAGATGGAAAAGGCGAGGGAGGCCGAG
GCGCGGGAAGCGCAACTGCGGGCCATTCTCGATACCGTACCGGATGCGATGGTGGTGATCGACGAGGATGGTTCGATCCG
CTCGTTCAGCGCGACCGCTGAAAAGATGTTCGGCTACACCGCGCAGGAGGTTCTCGGGCAGAACGTCGCGATGCTGATGC
CCGCGCCCGACAGTTCGCGCCACAGCGATTTCCTGCGCAGTTACCGGGAGACCGGGATCCGCCGCATCATGGGCCAGACC
CGCCGGGTGTTCGGCCGTCGCAAGGACGGCACGACATTTCCGCACGAGCTGACAATTGGCGAGGCGATGGGCGGAGGACG
ACGCCTGTTCACCGGCTTCCTGCGCGACCTGACCGAGCGCGAGGAGGCCGAGGAGCAGTTGCGCCAGCTTCAGGCCGAGC
TTGTCCGCATTTCCCGGATCTCGGCAGTGGGGACCATGGCGACCGCGCTTGCGCACGAGTTGAACCAGCCACTCACTGCA
ATAGCAAACTATGTCCTCACTTCCGCCACGCTGCTGGCCGATCCGGGCGAGGACACGATCGAGATAGTGCGCGAGGCGCT
GGAGGAGGCGGGGCAAGAGGCGGTGCGAGCAGGGGCGATCGTGCAGCGCCTGCGGGCTTTCGTGTCTCGCGGCGAACTGG
ACCGCACGATCGAATCCCCGGCAATTCTCGCGGCGGAGACGCTGGCCCTTTCCGGTCTTGCGGGGCGCCGCCGGCACGTG
TCGGTGGAAGTGGCGGTGCCCAGGACGCTCGGCGAAGTCCTCGTGGACAAGATCCAGATCCAGCAGGTGCTACTAAATCT
TGTGCGCAACGCGTTCGAGGCGCTGGAAGGACAAGGCCAGATACGCGTCGGCGCAAGGGAAATCGCGGGCGGCATGTTGC
AGTTCACCATCGAGGACGACGGTCCCGGCATCGACCCAGAACATCAGGCGGACCTGTTCGAACCTTTCGTCAGCACGAAG
GAGAACGGCATGGGCATCGGCCTCGCGATCTGTCGGACCATCGTCGAAAGCCACGGGGGCAAGCTCTGGCACGAGATGCG
GAATGACGGCGGCACTGCCTTCCATTTCACCATTCCCACCATCCGCGGAGCGCTCGAGAATGACTGA

Upstream 100 bases:

>100_bases
TGCGGGGTATGCGCGAAACTCCGCCTCAGGGGCAGATTGGGGGCAGGGGGTGCTGGAGCCAGGGATTGAAGCCGGGTTTG
GCGCGCTACAGGTAAGCAAC

Downstream 100 bases:

>100_bases
CAAGAAGCTGGTCCACGTCGTCGACGACGAGGAAGGCGTGCGGCGCTCGCTGGATTTCCTGCTGCGCACGACGGGGTACA
ACGTCCAGTGCTGGATCGAT

Product: PAS/PAC sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 608; Mature: 607

Protein sequence:

>608_residues
MPVAVAVFDREMRYIACSQLWLSEHRMAEADVVGRLHYDVFPDISDTWRAIHQRALAGETLSSRLDRYERADGSSDWVQW
NIQPWHTGKGDIGGVVMFARVVTNEIEADHQKHLLGRELDMLIDGAVDYAIFMLDEAGRVSLWNRGAERLTGWCQADIAQ
SHYEKLFVQADIDAALPILQLRMAEIVNSYRGRHFIARRDSTVILADVILTPIRSEGRLTGFGVVLRDVTGEMEKAREAE
AREAQLRAILDTVPDAMVVIDEDGSIRSFSATAEKMFGYTAQEVLGQNVAMLMPAPDSSRHSDFLRSYRETGIRRIMGQT
RRVFGRRKDGTTFPHELTIGEAMGGGRRLFTGFLRDLTEREEAEEQLRQLQAELVRISRISAVGTMATALAHELNQPLTA
IANYVLTSATLLADPGEDTIEIVREALEEAGQEAVRAGAIVQRLRAFVSRGELDRTIESPAILAAETLALSGLAGRRRHV
SVEVAVPRTLGEVLVDKIQIQQVLLNLVRNAFEALEGQGQIRVGAREIAGGMLQFTIEDDGPGIDPEHQADLFEPFVSTK
ENGMGIGLAICRTIVESHGGKLWHEMRNDGGTAFHFTIPTIRGALEND

Sequences:

>Translated_608_residues
MPVAVAVFDREMRYIACSQLWLSEHRMAEADVVGRLHYDVFPDISDTWRAIHQRALAGETLSSRLDRYERADGSSDWVQW
NIQPWHTGKGDIGGVVMFARVVTNEIEADHQKHLLGRELDMLIDGAVDYAIFMLDEAGRVSLWNRGAERLTGWCQADIAQ
SHYEKLFVQADIDAALPILQLRMAEIVNSYRGRHFIARRDSTVILADVILTPIRSEGRLTGFGVVLRDVTGEMEKAREAE
AREAQLRAILDTVPDAMVVIDEDGSIRSFSATAEKMFGYTAQEVLGQNVAMLMPAPDSSRHSDFLRSYRETGIRRIMGQT
RRVFGRRKDGTTFPHELTIGEAMGGGRRLFTGFLRDLTEREEAEEQLRQLQAELVRISRISAVGTMATALAHELNQPLTA
IANYVLTSATLLADPGEDTIEIVREALEEAGQEAVRAGAIVQRLRAFVSRGELDRTIESPAILAAETLALSGLAGRRRHV
SVEVAVPRTLGEVLVDKIQIQQVLLNLVRNAFEALEGQGQIRVGAREIAGGMLQFTIEDDGPGIDPEHQADLFEPFVSTK
ENGMGIGLAICRTIVESHGGKLWHEMRNDGGTAFHFTIPTIRGALEND
>Mature_607_residues
PVAVAVFDREMRYIACSQLWLSEHRMAEADVVGRLHYDVFPDISDTWRAIHQRALAGETLSSRLDRYERADGSSDWVQWN
IQPWHTGKGDIGGVVMFARVVTNEIEADHQKHLLGRELDMLIDGAVDYAIFMLDEAGRVSLWNRGAERLTGWCQADIAQS
HYEKLFVQADIDAALPILQLRMAEIVNSYRGRHFIARRDSTVILADVILTPIRSEGRLTGFGVVLRDVTGEMEKAREAEA
REAQLRAILDTVPDAMVVIDEDGSIRSFSATAEKMFGYTAQEVLGQNVAMLMPAPDSSRHSDFLRSYRETGIRRIMGQTR
RVFGRRKDGTTFPHELTIGEAMGGGRRLFTGFLRDLTEREEAEEQLRQLQAELVRISRISAVGTMATALAHELNQPLTAI
ANYVLTSATLLADPGEDTIEIVREALEEAGQEAVRAGAIVQRLRAFVSRGELDRTIESPAILAAETLALSGLAGRRRHVS
VEVAVPRTLGEVLVDKIQIQQVLLNLVRNAFEALEGQGQIRVGAREIAGGMLQFTIEDDGPGIDPEHQADLFEPFVSTKE
NGMGIGLAICRTIVESHGGKLWHEMRNDGGTAFHFTIPTIRGALEND

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=357, Percent_Identity=29.1316526610644, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI1790436, Length=230, Percent_Identity=29.5652173913043, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1790300, Length=366, Percent_Identity=27.0491803278689, Blast_Score=83, Evalue=6e-17,
Organism=Escherichia coli, GI1786912, Length=225, Percent_Identity=28.4444444444444, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI48994928, Length=404, Percent_Identity=23.7623762376238, Blast_Score=77, Evalue=5e-15,
Organism=Escherichia coli, GI1786600, Length=378, Percent_Identity=23.8095238095238, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI87081921, Length=153, Percent_Identity=26.797385620915, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1790346, Length=227, Percent_Identity=27.7533039647577, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI1789808, Length=218, Percent_Identity=27.0642201834862, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI87081816, Length=253, Percent_Identity=28.4584980237154, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 67499; Mature: 67367

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVAVAVFDREMRYIACSQLWLSEHRMAEADVVGRLHYDVFPDISDTWRAIHQRALAGET
CCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
LSSRLDRYERADGSSDWVQWNIQPWHTGKGDIGGVVMFARVVTNEIEADHQKHLLGRELD
HHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLIDGAVDYAIFMLDEAGRVSLWNRGAERLTGWCQADIAQSHYEKLFVQADIDAALPILQ
HHHHHHHHEEEEEECCCCCEEHHHCCHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHH
LRMAEIVNSYRGRHFIARRDSTVILADVILTPIRSEGRLTGFGVVLRDVTGEMEKAREAE
HHHHHHHHHHCCCEEEEECCCCEEEEHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHH
AREAQLRAILDTVPDAMVVIDEDGSIRSFSATAEKMFGYTAQEVLGQNVAMLMPAPDSSR
HHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCHHHHHHCCCCEEEECCCCCCH
HSDFLRSYRETGIRRIMGQTRRVFGRRKDGTTFPHELTIGEAMGGGRRLFTGFLRDLTER
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHCCCCHHHHHHHHHHHHHH
EEAEEQLRQLQAELVRISRISAVGTMATALAHELNQPLTAIANYVLTSATLLADPGEDTI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHH
EIVREALEEAGQEAVRAGAIVQRLRAFVSRGELDRTIESPAILAAETLALSGLAGRRRHV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHCCCCCCEEE
SVEVAVPRTLGEVLVDKIQIQQVLLNLVRNAFEALEGQGQIRVGAREIAGGMLQFTIEDD
EEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHCCCEEEEEECCC
GPGIDPEHQADLFEPFVSTKENGMGIGLAICRTIVESHGGKLWHEMRNDGGTAFHFTIPT
CCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEECHH
IRGALEND
HHCCCCCC
>Mature Secondary Structure 
PVAVAVFDREMRYIACSQLWLSEHRMAEADVVGRLHYDVFPDISDTWRAIHQRALAGET
CEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
LSSRLDRYERADGSSDWVQWNIQPWHTGKGDIGGVVMFARVVTNEIEADHQKHLLGRELD
HHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLIDGAVDYAIFMLDEAGRVSLWNRGAERLTGWCQADIAQSHYEKLFVQADIDAALPILQ
HHHHHHHHEEEEEECCCCCEEHHHCCHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHH
LRMAEIVNSYRGRHFIARRDSTVILADVILTPIRSEGRLTGFGVVLRDVTGEMEKAREAE
HHHHHHHHHHCCCEEEEECCCCEEEEHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHH
AREAQLRAILDTVPDAMVVIDEDGSIRSFSATAEKMFGYTAQEVLGQNVAMLMPAPDSSR
HHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCHHHHHHCCCCEEEECCCCCCH
HSDFLRSYRETGIRRIMGQTRRVFGRRKDGTTFPHELTIGEAMGGGRRLFTGFLRDLTER
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHCCCCHHHHHHHHHHHHHH
EEAEEQLRQLQAELVRISRISAVGTMATALAHELNQPLTAIANYVLTSATLLADPGEDTI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHH
EIVREALEEAGQEAVRAGAIVQRLRAFVSRGELDRTIESPAILAAETLALSGLAGRRRHV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHCCCCCCEEE
SVEVAVPRTLGEVLVDKIQIQQVLLNLVRNAFEALEGQGQIRVGAREIAGGMLQFTIEDD
EEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHCCCEEEEEECCC
GPGIDPEHQADLFEPFVSTKENGMGIGLAICRTIVESHGGKLWHEMRNDGGTAFHFTIPT
CCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEECHH
IRGALEND
HHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2046550; 1766374 [H]