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The map label for this gene is fixJ [H]

Identifier: 87199633

GI number: 87199633

Start: 1686073

End: 1686729

Strand: Direct

Name: fixJ [H]

Synonym: Saro_1616

Alternate gene names: 87199633

Gene position: 1686073-1686729 (Clockwise)

Preceding gene: 87199632

Following gene: 87199636

Centisome position: 47.34

GC content: 61.49

Gene sequence:

>657_bases
ATGACTGACAAGAAGCTGGTCCACGTCGTCGACGACGAGGAAGGCGTGCGGCGCTCGCTGGATTTCCTGCTGCGCACGAC
GGGGTACAACGTCCAGTGCTGGATCGATGGCGATACCTTTCTCAAGGATGTGGACGGTTCGGTTCCGGCGTGCGTGCTGA
TGGATATCCGCATGCCCGGGTCATCGGGGCTGGAGATCCAGGAACAGATGCCCGAGCATGGACTGGACTTTCCGGTCATC
GTGCTTTCGGGCCATGGAGACATTCACTCGGCGGTAAGGGCGATGAAGGCTGGCGCAGTCGATTTCGTCGAGAAGCCGTT
CAGTCGGGACAAGCTGCTCGATGCCATCGCGAATGCTTTCCAGCGCGTCGAGAACGGCGAGGCGGCGCGTGAGAAGGCGG
TATCGGCGCAGACCCTGGTAGCAAGGCTGACCTCGCGCGAGCAGGAGGTGCTCGAGGGGCTCGCCTGCGGATATCCGAAC
AAGACCATCGCCTACGATCTCGGTATCAGTCCCCGTACGGTCGAGGTTTATCGCGCCAATATCATGACCAAGCTCAAGGT
TCCCAGCTTTGCCGATGCCCTGCGCATTGCGTTCACGGCCGGGCTTGGAATGGAAGCGCAGTGGCGCAAGGAGCACTCCA
CCCAGCGGCTCGGCTGA

Upstream 100 bases:

>100_bases
ATCGTCGAAAGCCACGGGGGCAAGCTCTGGCACGAGATGCGGAATGACGGCGGCACTGCCTTCCATTTCACCATTCCCAC
CATCCGCGGAGCGCTCGAGA

Downstream 100 bases:

>100_bases
TTCCCTTTCCGGAACAAACCGCATTATCCGTATCTCGCGCTCTGGGTAGCGTGGCGGTGCAGGTATGCCGGCCCTCGACC
CCACCCCCCGTCCCCGGCTG

Product: two component LuxR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MTDKKLVHVVDDEEGVRRSLDFLLRTTGYNVQCWIDGDTFLKDVDGSVPACVLMDIRMPGSSGLEIQEQMPEHGLDFPVI
VLSGHGDIHSAVRAMKAGAVDFVEKPFSRDKLLDAIANAFQRVENGEAAREKAVSAQTLVARLTSREQEVLEGLACGYPN
KTIAYDLGISPRTVEVYRANIMTKLKVPSFADALRIAFTAGLGMEAQWRKEHSTQRLG

Sequences:

>Translated_218_residues
MTDKKLVHVVDDEEGVRRSLDFLLRTTGYNVQCWIDGDTFLKDVDGSVPACVLMDIRMPGSSGLEIQEQMPEHGLDFPVI
VLSGHGDIHSAVRAMKAGAVDFVEKPFSRDKLLDAIANAFQRVENGEAAREKAVSAQTLVARLTSREQEVLEGLACGYPN
KTIAYDLGISPRTVEVYRANIMTKLKVPSFADALRIAFTAGLGMEAQWRKEHSTQRLG
>Mature_217_residues
TDKKLVHVVDDEEGVRRSLDFLLRTTGYNVQCWIDGDTFLKDVDGSVPACVLMDIRMPGSSGLEIQEQMPEHGLDFPVIV
LSGHGDIHSAVRAMKAGAVDFVEKPFSRDKLLDAIANAFQRVENGEAAREKAVSAQTLVARLTSREQEVLEGLACGYPNK
TIAYDLGISPRTVEVYRANIMTKLKVPSFADALRIAFTAGLGMEAQWRKEHSTQRLG

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=121, Percent_Identity=36.3636363636364, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790102, Length=154, Percent_Identity=37.012987012987, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1790299, Length=135, Percent_Identity=29.6296296296296, Blast_Score=75, Evalue=2e-15,
Organism=Escherichia coli, GI1788222, Length=210, Percent_Identity=27.1428571428571, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1786599, Length=139, Percent_Identity=28.7769784172662, Blast_Score=66, Evalue=1e-12,
Organism=Escherichia coli, GI1790437, Length=155, Percent_Identity=27.0967741935484, Blast_Score=60, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 24035; Mature: 23904

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKKLVHVVDDEEGVRRSLDFLLRTTGYNVQCWIDGDTFLKDVDGSVPACVLMDIRMPG
CCCCEEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCHHHHHCCCCCCEEEEEEEECCC
SSGLEIQEQMPEHGLDFPVIVLSGHGDIHSAVRAMKAGAVDFVEKPFSRDKLLDAIANAF
CCCCHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHH
QRVENGEAAREKAVSAQTLVARLTSREQEVLEGLACGYPNKTIAYDLGISPRTVEVYRAN
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEEHH
IMTKLKVPSFADALRIAFTAGLGMEAQWRKEHSTQRLG
HHEEECCCCHHHHHHHHHHHCCCCCHHHHHHCCCCCCC
>Mature Secondary Structure 
TDKKLVHVVDDEEGVRRSLDFLLRTTGYNVQCWIDGDTFLKDVDGSVPACVLMDIRMPG
CCCEEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCHHHHHCCCCCCEEEEEEEECCC
SSGLEIQEQMPEHGLDFPVIVLSGHGDIHSAVRAMKAGAVDFVEKPFSRDKLLDAIANAF
CCCCHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHH
QRVENGEAAREKAVSAQTLVARLTSREQEVLEGLACGYPNKTIAYDLGISPRTVEVYRAN
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEEHH
IMTKLKVPSFADALRIAFTAGLGMEAQWRKEHSTQRLG
HHEEECCCCHHHHHHHHHHHCCCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275 [H]