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The map label for this gene is fixJ [H]

Identifier: 87199631

GI number: 87199631

Start: 1683390

End: 1684055

Strand: Direct

Name: fixJ [H]

Synonym: Saro_1614

Alternate gene names: 87199631

Gene position: 1683390-1684055 (Clockwise)

Preceding gene: 87199630

Following gene: 87199632

Centisome position: 47.27

GC content: 63.51

Gene sequence:

>666_bases
ATGGCTGAGGCCCCGATCGTATACGTAGTCGATGATGATGCCAGCGTCCGAGCTTCGATCGGCTTCACCATGCGGAGCGT
CGGTCGCCGTGCCATATGTTTCGAGAGCGGCCAGGGCCTGCTCGAACTCGCCGCGGCCCTTCCGCCCGCGGTCGTCCTGC
TGGACGTCGGCCAGGATGGTTCTGAAGGAGTGAGTGTCCAGCGCGAACTGTGGCGCAGAGGCATCGACTTTCCAGTCGTT
GCGATGGTGGGCAACAGCGATACCCAGACCGCCGTGAACGCGATCAACGCCGGTGTCAGCGATTTCCTGCTCAAGCCGTT
TTCAGCCCAGGATCTCATTGCAACGGTGATCGAGGCCGAGCGCCGTTTTCGCAGTCCGGAACGACAACACGCGCGTGCGC
AGGAGGCGCGGGCGATGCTTGAACGGCTGACCGCGCCCGAACGCCGCGTTCTTGCTGCCGTCACCAGCGGCATGCCCAAT
GCGGAAATCGCAGACGAACTGGGCATTTCGATCAGGACGGTCGAGGTTCATCGCGCGATGATCGTGCGCAAGTTCGAGGT
CCAGTCCTTCACCTCGGTTCTCCGGATCGCCTTGGGAGGTCGGGTCACGCATTGCCCGACCGCGCCGCGTCCGCCTTTGC
ATGATGCGGTCGCTGCCGGGCGATAA

Upstream 100 bases:

>100_bases
GATCTCGACTGTAGTATTATTTTTTAATTAATGATCCGAATCGGTCAGATGCGGACGTTGAAGATCGGGTGGCGACGCTT
TGTACTCACAGGAGCAGTCG

Downstream 100 bases:

>100_bases
CCGGGGAGGCGCGGGGGTCGTGGATTTTCTTGGGATATCTACGAATGGCGCAGGGAGGTGAATGACCTAGTCCGTAAGAA
ACGGGTCAGTGCTGCCATTG

Product: two component LuxR family transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MAEAPIVYVVDDDASVRASIGFTMRSVGRRAICFESGQGLLELAAALPPAVVLLDVGQDGSEGVSVQRELWRRGIDFPVV
AMVGNSDTQTAVNAINAGVSDFLLKPFSAQDLIATVIEAERRFRSPERQHARAQEARAMLERLTAPERRVLAAVTSGMPN
AEIADELGISIRTVEVHRAMIVRKFEVQSFTSVLRIALGGRVTHCPTAPRPPLHDAVAAGR

Sequences:

>Translated_221_residues
MAEAPIVYVVDDDASVRASIGFTMRSVGRRAICFESGQGLLELAAALPPAVVLLDVGQDGSEGVSVQRELWRRGIDFPVV
AMVGNSDTQTAVNAINAGVSDFLLKPFSAQDLIATVIEAERRFRSPERQHARAQEARAMLERLTAPERRVLAAVTSGMPN
AEIADELGISIRTVEVHRAMIVRKFEVQSFTSVLRIALGGRVTHCPTAPRPPLHDAVAAGR
>Mature_220_residues
AEAPIVYVVDDDASVRASIGFTMRSVGRRAICFESGQGLLELAAALPPAVVLLDVGQDGSEGVSVQRELWRRGIDFPVVA
MVGNSDTQTAVNAINAGVSDFLLKPFSAQDLIATVIEAERRFRSPERQHARAQEARAMLERLTAPERRVLAAVTSGMPNA
EIADELGISIRTVEVHRAMIVRKFEVQSFTSVLRIALGGRVTHCPTAPRPPLHDAVAAGR

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=127, Percent_Identity=33.8582677165354, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1788550, Length=106, Percent_Identity=29.2452830188679, Blast_Score=60, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23835; Mature: 23703

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEAPIVYVVDDDASVRASIGFTMRSVGRRAICFESGQGLLELAAALPPAVVLLDVGQDG
CCCCCEEEEECCCCCEEHHHHHHHHHCCCEEEEEECCCCHHHHHHHCCCEEEEEECCCCC
SEGVSVQRELWRRGIDFPVVAMVGNSDTQTAVNAINAGVSDFLLKPFSAQDLIATVIEAE
CCCHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHH
RRFRSPERQHARAQEARAMLERLTAPERRVLAAVTSGMPNAEIADELGISIRTVEVHRAM
HHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHCCEEEEHHHHHHH
IVRKFEVQSFTSVLRIALGGRVTHCPTAPRPPLHDAVAAGR
HHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHCCC
>Mature Secondary Structure 
AEAPIVYVVDDDASVRASIGFTMRSVGRRAICFESGQGLLELAAALPPAVVLLDVGQDG
CCCCEEEEECCCCCEEHHHHHHHHHCCCEEEEEECCCCHHHHHHHCCCEEEEEECCCCC
SEGVSVQRELWRRGIDFPVVAMVGNSDTQTAVNAINAGVSDFLLKPFSAQDLIATVIEAE
CCCHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHH
RRFRSPERQHARAQEARAMLERLTAPERRVLAAVTSGMPNAEIADELGISIRTVEVHRAM
HHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHCCEEEEHHHHHHH
IVRKFEVQSFTSVLRIALGGRVTHCPTAPRPPLHDAVAAGR
HHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2842062; 11481432; 2663474; 1857213; 10647182 [H]