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The map label for this gene is ligD [H]

Identifier: 87198815

GI number: 87198815

Start: 842319

End: 843251

Strand: Reverse

Name: ligD [H]

Synonym: Saro_0793

Alternate gene names: 87198815

Gene position: 843251-842319 (Counterclockwise)

Preceding gene: 87198816

Following gene: 87198812

Centisome position: 23.68

GC content: 63.77

Gene sequence:

>933_bases
ATGAAGGACTTCGCGGGCCGTACCGCCTTCGTCACGGGCGGCGCCAATGGCGTCGGCATCGGCCTTGTCCGCCAGCTCCT
CAACCAGGGGTGCAAGGTCGCCATCGCCGACATCAGGCAGGATTCGATCGACAAGGCTCTGGCCACGCTGGAGGCGGAAG
GATCTGGGCCCGAAGTCATGGGCGTCCAGCTCGACGTGGCAAGCCGCGAGGGCTTCAAGATGGCGGCGGACGAGGTCGAG
GCCAGGTTCGGTCCGGTGTCGATCCTGTGCAACAATGCCGGGGTAAACCTGTTCCAGCCGATCGAGGAATCTTCCTACGA
CGACTGGGACTGGCTGCTGGGCGTGAACCTCCACGGCGTGGTAAACGGGGTGACGACCTTCGTTCCGCGCATGGTCGAGC
GGGTGAAGGCAGGGGAGCAGAAGGGCGGACACGTGGTCAACACCGCGTCGATGGCGGCCTTCCTCGCGGCGGGAAGCCCC
GGCATCTACAATACCACCAAGTTCGCGGTGCGCGGCCTTTCGGAATCGCTGCACTATTCGCTGCTCAAGTACGAGATCGG
GGTTTCGGTACTCTGCCCGGGCCTGGTGAAGAGCTATATCTATGCCAGCGACGACATCCGTCCGGATGCGCTGAAGGGCG
AGATGAAACCAGTGGACAAGACCGCGGTCGAACGGCTTGCGGGCGTGCACGAGTTCGGCATGGAACCGGACGTGATCGGC
GCGCGGGTGATCGAGGCGATGAAGGCCAACCGCCTGCACATCTTCTCGCACCCGGATCACAAGGAAGAGCTGCGCGAGAT
CTTCGACGAGATCATCGCCGAGTATCAGGACTACCCCAAGGACCCCGGCTACGACCAGCGCGTGGCTTTCGAGAAGTTCC
GGGCCGACAGCTTTGCCGAAGCGCGGCGGCAGTCGAGGGCGGCGGATTTCTGA

Upstream 100 bases:

>100_bases
ACAAGGAGACCTTCGCAGCCCTGTTCCGCAATCCGGCGATCACCGACGAAATCGCCCGGCAGAACAAGCTGAAGGAAACC
CGCAAGGCCGGGGTCGAGGC

Downstream 100 bases:

>100_bases
GCGCTGCGACCCCACACGGATAATAAAAAAGGGCGGAGCCGCGAGGCCCCGCCCTTTCTCTATCCGCTCACTGAAACCTT
ACGCGACGTCGCGCAGGGCT

Product: short-chain dehydrogenase/reductase SDR

Products: D-Gluconate; Nicotinamide adenine dinucleotide phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MKDFAGRTAFVTGGANGVGIGLVRQLLNQGCKVAIADIRQDSIDKALATLEAEGSGPEVMGVQLDVASREGFKMAADEVE
ARFGPVSILCNNAGVNLFQPIEESSYDDWDWLLGVNLHGVVNGVTTFVPRMVERVKAGEQKGGHVVNTASMAAFLAAGSP
GIYNTTKFAVRGLSESLHYSLLKYEIGVSVLCPGLVKSYIYASDDIRPDALKGEMKPVDKTAVERLAGVHEFGMEPDVIG
ARVIEAMKANRLHIFSHPDHKEELREIFDEIIAEYQDYPKDPGYDQRVAFEKFRADSFAEARRQSRAADF

Sequences:

>Translated_310_residues
MKDFAGRTAFVTGGANGVGIGLVRQLLNQGCKVAIADIRQDSIDKALATLEAEGSGPEVMGVQLDVASREGFKMAADEVE
ARFGPVSILCNNAGVNLFQPIEESSYDDWDWLLGVNLHGVVNGVTTFVPRMVERVKAGEQKGGHVVNTASMAAFLAAGSP
GIYNTTKFAVRGLSESLHYSLLKYEIGVSVLCPGLVKSYIYASDDIRPDALKGEMKPVDKTAVERLAGVHEFGMEPDVIG
ARVIEAMKANRLHIFSHPDHKEELREIFDEIIAEYQDYPKDPGYDQRVAFEKFRADSFAEARRQSRAADF
>Mature_310_residues
MKDFAGRTAFVTGGANGVGIGLVRQLLNQGCKVAIADIRQDSIDKALATLEAEGSGPEVMGVQLDVASREGFKMAADEVE
ARFGPVSILCNNAGVNLFQPIEESSYDDWDWLLGVNLHGVVNGVTTFVPRMVERVKAGEQKGGHVVNTASMAAFLAAGSP
GIYNTTKFAVRGLSESLHYSLLKYEIGVSVLCPGLVKSYIYASDDIRPDALKGEMKPVDKTAVERLAGVHEFGMEPDVIG
ARVIEAMKANRLHIFSHPDHKEELREIFDEIIAEYQDYPKDPGYDQRVAFEKFRADSFAEARRQSRAADF

Specific function: Catalyzes the C alpha dehydrogenation of arylglycerol- beta-aryl ether (C alpha alcohol type) (compound IV) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI32483357, Length=198, Percent_Identity=32.8282828282828, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI5031737, Length=238, Percent_Identity=28.5714285714286, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI31542939, Length=200, Percent_Identity=29, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI33667109, Length=196, Percent_Identity=30.6122448979592, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI7657478, Length=194, Percent_Identity=29.8969072164948, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI224922801, Length=171, Percent_Identity=29.2397660818713, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI126723191, Length=191, Percent_Identity=30.3664921465969, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI44680136, Length=221, Percent_Identity=28.0542986425339, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI44680133, Length=221, Percent_Identity=28.0542986425339, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI17738292, Length=221, Percent_Identity=28.0542986425339, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI126723750, Length=201, Percent_Identity=28.3582089552239, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI40807363, Length=228, Percent_Identity=24.5614035087719, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI7706318, Length=216, Percent_Identity=26.3888888888889, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI142976729, Length=200, Percent_Identity=26, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI22507405, Length=207, Percent_Identity=28.9855072463768, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI7705925, Length=197, Percent_Identity=29.4416243654822, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI109715829, Length=202, Percent_Identity=24.7524752475248, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1790717, Length=196, Percent_Identity=28.0612244897959, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI87082100, Length=205, Percent_Identity=31.7073170731707, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1787820, Length=206, Percent_Identity=29.126213592233, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI87082160, Length=247, Percent_Identity=26.3157894736842, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789208, Length=195, Percent_Identity=26.1538461538462, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1788459, Length=206, Percent_Identity=23.7864077669903, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787335, Length=211, Percent_Identity=24.6445497630332, Blast_Score=65, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17561402, Length=200, Percent_Identity=31, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17567345, Length=212, Percent_Identity=28.7735849056604, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17560676, Length=199, Percent_Identity=27.1356783919598, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI193203115, Length=202, Percent_Identity=26.7326732673267, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17508895, Length=203, Percent_Identity=25.1231527093596, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17538182, Length=241, Percent_Identity=26.5560165975104, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI193203117, Length=191, Percent_Identity=27.2251308900524, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17538486, Length=206, Percent_Identity=29.6116504854369, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17562910, Length=221, Percent_Identity=24.8868778280543, Blast_Score=64, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17560220, Length=219, Percent_Identity=26.027397260274, Blast_Score=64, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6323882, Length=199, Percent_Identity=26.1306532663317, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI21355631, Length=182, Percent_Identity=31.3186813186813, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI281364892, Length=263, Percent_Identity=24.7148288973384, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24665243, Length=206, Percent_Identity=30.5825242718447, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI221331218, Length=206, Percent_Identity=30.5825242718447, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24665247, Length=207, Percent_Identity=30.9178743961353, Blast_Score=78, Evalue=9e-15,
Organism=Drosophila melanogaster, GI221331216, Length=207, Percent_Identity=30.9178743961353, Blast_Score=78, Evalue=9e-15,
Organism=Drosophila melanogaster, GI21355319, Length=243, Percent_Identity=27.1604938271605, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24643142, Length=202, Percent_Identity=29.7029702970297, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI21356301, Length=195, Percent_Identity=28.7179487179487, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21358495, Length=250, Percent_Identity=24, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24581726, Length=253, Percent_Identity=25.6916996047431, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24639444, Length=230, Percent_Identity=25.6521739130435, Blast_Score=64, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.69 [C]

Molecular weight: Translated: 33860; Mature: 33860

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDFAGRTAFVTGGANGVGIGLVRQLLNQGCKVAIADIRQDSIDKALATLEAEGSGPEVM
CCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHCCCCCCCEEE
GVQLDVASREGFKMAADEVEARFGPVSILCNNAGVNLFQPIEESSYDDWDWLLGVNLHGV
EEEEEECCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHCCCCCCCEEECCCHHHH
VNGVTTFVPRMVERVKAGEQKGGHVVNTASMAAFLAAGSPGIYNTTKFAVRGLSESLHYS
HHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
LLKYEIGVSVLCPGLVKSYIYASDDIRPDALKGEMKPVDKTAVERLAGVHEFGMEPDVIG
HHHHHHCHHHHCHHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHH
ARVIEAMKANRLHIFSHPDHKEELREIFDEIIAEYQDYPKDPGYDQRVAFEKFRADSFAE
HHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCHHHHH
ARRQSRAADF
HHHHHCCCCC
>Mature Secondary Structure
MKDFAGRTAFVTGGANGVGIGLVRQLLNQGCKVAIADIRQDSIDKALATLEAEGSGPEVM
CCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHCCCCCCCEEE
GVQLDVASREGFKMAADEVEARFGPVSILCNNAGVNLFQPIEESSYDDWDWLLGVNLHGV
EEEEEECCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHCCCCCCCEEECCCHHHH
VNGVTTFVPRMVERVKAGEQKGGHVVNTASMAAFLAAGSPGIYNTTKFAVRGLSESLHYS
HHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
LLKYEIGVSVLCPGLVKSYIYASDDIRPDALKGEMKPVDKTAVERLAGVHEFGMEPDVIG
HHHHHHCHHHHCHHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHH
ARVIEAMKANRLHIFSHPDHKEELREIFDEIIAEYQDYPKDPGYDQRVAFEKFRADSFAE
HHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCHHHHH
ARRQSRAADF
HHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+; NADH; NADP+; NADPH [C]

Metal ions: Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.02 {NADP+}} 20 {D-gluconate}} 0.006 {NADPH}} 0.9 {5-keto-D-gluconate}} [C]

Substrates: 5-Dehydro-D-gluconate; H+; Nicotinamide adenine dinucleotide phosphate - reduced [C]

Specific reaction: 5-Dehydro-D-gluconate + H+ + Nicotinamide adenine dinucleotide phosphate - reduced <==> D-Gluconate + Nicotinamide adenine dinucleotide phosphate [C]

General reaction: Redox reaction [C]

Inhibitor: Cd2+; Cs2+; Cu2+; Divalent metal ions; Hg2+; Ni2+; p-Chloromercuribenzoate; Sn2+; Sulfhydryl reagents [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1744051; 8495726; 7764263 [H]