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The map label for this gene is btuE [H]

Identifier: 87198589

GI number: 87198589

Start: 612965

End: 613513

Strand: Reverse

Name: btuE [H]

Synonym: Saro_0565

Alternate gene names: 87198589

Gene position: 613513-612965 (Counterclockwise)

Preceding gene: 87198590

Following gene: 87198588

Centisome position: 17.23

GC content: 66.67

Gene sequence:

>549_bases
TTGCCGGATACCCTCGCCGCCATACCGCTCACCCGCATCGACGGAACCGCGGACAGCCTCGCGAACCATGCCGGCAAGGT
GCTGCTGGTGGTCAACGTCGCATCGAAGTGCGGCCTGACCCCGCAGTACGAGGGACTCGAGGCGCTCTACAAGGCGCGCA
AGGACGAGGGGCTCGAGGTGCTGGGCTTTCCGGCCAACGACTTTGGCGCGCAGGAGCCCGGGTCGCACGAGGAAATCGCC
GAGTTCTGCAAGCTCAACTATGGCGTTTCCTTCCCGCTTTTCGCCAAGGCCGACGTGACAGGCCCGGGCAAGCAGCCGCT
TTACGCCGCGCTGACCGAAGCCGCGCCAACGAAGCAGGGGCCGGCGCAGGAATTCCGCGACCGGCTCAAGGGTTATGGCA
TGACGCCGACCGAGGACCCGGAAGTGCTGTGGAACTTCGAGAAGTTCGTGATCGGACGCGACGGCGAGGTCGCGGCGCGC
TTTGCCCCCGGCGTCACCCCTGACGACCCCGCGCTGCTGGCAGCGATCGACGCCGAACTGGCAAAGTGA

Upstream 100 bases:

>100_bases
CTCCCTTCCGCCTGGTCGAACTGGCACCGGGCGTCACCGCCGACGAGGTGCGCGCCAGAACGACGGCGCACTACGTCGAG
TAACAAAGGGAGAAACTGCC

Downstream 100 bases:

>100_bases
CCGCGTGAGCTACGTCCTCGTCACGGCGAACCGCAATTATTCGAGCTGGTCGCTGCGCCCGTGGGTGCTGATGAAGGCGC
TCGGCATTCCTTTCGAGGAC

Product: glutathione peroxidase

Products: ADP; phosphate; cob(I)alamin [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 182; Mature: 181

Protein sequence:

>182_residues
MPDTLAAIPLTRIDGTADSLANHAGKVLLVVNVASKCGLTPQYEGLEALYKARKDEGLEVLGFPANDFGAQEPGSHEEIA
EFCKLNYGVSFPLFAKADVTGPGKQPLYAALTEAAPTKQGPAQEFRDRLKGYGMTPTEDPEVLWNFEKFVIGRDGEVAAR
FAPGVTPDDPALLAAIDAELAK

Sequences:

>Translated_182_residues
MPDTLAAIPLTRIDGTADSLANHAGKVLLVVNVASKCGLTPQYEGLEALYKARKDEGLEVLGFPANDFGAQEPGSHEEIA
EFCKLNYGVSFPLFAKADVTGPGKQPLYAALTEAAPTKQGPAQEFRDRLKGYGMTPTEDPEVLWNFEKFVIGRDGEVAAR
FAPGVTPDDPALLAAIDAELAK
>Mature_181_residues
PDTLAAIPLTRIDGTADSLANHAGKVLLVVNVASKCGLTPQYEGLEALYKARKDEGLEVLGFPANDFGAQEPGSHEEIAE
FCKLNYGVSFPLFAKADVTGPGKQPLYAALTEAAPTKQGPAQEFRDRLKGYGMTPTEDPEVLWNFEKFVIGRDGEVAARF
APGVTPDDPALLAAIDAELAK

Specific function: Not essential for B12 transport; however, it is an auxiliary component of the transport system [H]

COG id: COG0386

COG function: function code O; Glutathione peroxidase

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutathione peroxidase family [H]

Homologues:

Organism=Homo sapiens, GI15618997, Length=158, Percent_Identity=37.3417721518987, Blast_Score=101, Evalue=3e-22,
Organism=Homo sapiens, GI4557629, Length=153, Percent_Identity=39.2156862745098, Blast_Score=100, Evalue=6e-22,
Organism=Homo sapiens, GI41406084, Length=171, Percent_Identity=38.0116959064327, Blast_Score=91, Evalue=6e-19,
Organism=Homo sapiens, GI6006001, Length=161, Percent_Identity=37.888198757764, Blast_Score=90, Evalue=1e-18,
Organism=Homo sapiens, GI192455698, Length=180, Percent_Identity=31.6666666666667, Blast_Score=90, Evalue=1e-18,
Organism=Homo sapiens, GI90903240, Length=156, Percent_Identity=36.5384615384615, Blast_Score=88, Evalue=5e-18,
Organism=Homo sapiens, GI75709200, Length=156, Percent_Identity=37.8205128205128, Blast_Score=88, Evalue=5e-18,
Organism=Homo sapiens, GI33186887, Length=137, Percent_Identity=38.6861313868613, Blast_Score=85, Evalue=4e-17,
Organism=Homo sapiens, GI32967607, Length=182, Percent_Identity=32.967032967033, Blast_Score=76, Evalue=1e-14,
Organism=Homo sapiens, GI90903238, Length=138, Percent_Identity=36.9565217391304, Blast_Score=75, Evalue=3e-14,
Organism=Escherichia coli, GI1788003, Length=183, Percent_Identity=50.2732240437158, Blast_Score=190, Evalue=5e-50,
Organism=Caenorhabditis elegans, GI17535473, Length=180, Percent_Identity=38.3333333333333, Blast_Score=110, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17506887, Length=157, Percent_Identity=38.2165605095541, Blast_Score=102, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17550320, Length=179, Percent_Identity=31.2849162011173, Blast_Score=88, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI193209493, Length=179, Percent_Identity=30.1675977653631, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71993584, Length=167, Percent_Identity=28.7425149700599, Blast_Score=84, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI193210707, Length=157, Percent_Identity=34.3949044585987, Blast_Score=80, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6322228, Length=158, Percent_Identity=46.8354430379747, Blast_Score=141, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6319721, Length=158, Percent_Identity=44.9367088607595, Blast_Score=136, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6322826, Length=150, Percent_Identity=38.6666666666667, Blast_Score=110, Evalue=2e-25,
Organism=Drosophila melanogaster, GI85726443, Length=176, Percent_Identity=34.6590909090909, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24656772, Length=160, Percent_Identity=38.125, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24656767, Length=160, Percent_Identity=38.125, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24656777, Length=160, Percent_Identity=38.125, Blast_Score=85, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000889
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00255 GSHPx [H]

EC number: NA

Molecular weight: Translated: 19404; Mature: 19273

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS00460 GLUTATHIONE_PEROXID_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDTLAAIPLTRIDGTADSLANHAGKVLLVVNVASKCGLTPQYEGLEALYKARKDEGLEV
CCCCCCCCCHHHCCCCHHHHHHHCCCEEEEEEEHHHCCCCCCHHHHHHHHHHHCCCCCEE
LGFPANDFGAQEPGSHEEIAEFCKLNYGVSFPLFAKADVTGPGKQPLYAALTEAAPTKQG
EECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCHHHHHHHCCCCCCC
PAQEFRDRLKGYGMTPTEDPEVLWNFEKFVIGRDGEVAARFAPGVTPDDPALLAAIDAEL
CHHHHHHHHHCCCCCCCCCHHHEECHHHEEECCCCCEEEEECCCCCCCCCCEEHHHHHHH
AK
CC
>Mature Secondary Structure 
PDTLAAIPLTRIDGTADSLANHAGKVLLVVNVASKCGLTPQYEGLEALYKARKDEGLEV
CCCCCCCCHHHCCCCHHHHHHHCCCEEEEEEEHHHCCCCCCHHHHHHHHHHHCCCCCEE
LGFPANDFGAQEPGSHEEIAEFCKLNYGVSFPLFAKADVTGPGKQPLYAALTEAAPTKQG
EECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCHHHHHHHCCCCCCC
PAQEFRDRLKGYGMTPTEDPEVLWNFEKFVIGRDGEVAARFAPGVTPDDPALLAAIDAEL
CHHHHHHHHHCCCCCCCCCHHHEECHHHEEECCCCCEEEEECCCCCCCCCCEEHHHHHHH
AK
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; cob(I)alamin [Periplasm]; H2O [C]

Specific reaction: ATP + cob(I)alamin [Periplasm] + H2O = ADP + phosphate + cob(I)alamin [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3528129; 9097039; 9278503 [H]