Click here to switch to the map view.

The map label for this gene is lpsJ [H]

Identifier: 87198590

GI number: 87198590

Start: 613531

End: 614190

Strand: Reverse

Name: lpsJ [H]

Synonym: Saro_0566

Alternate gene names: 87198590

Gene position: 614190-613531 (Counterclockwise)

Preceding gene: 87198591

Following gene: 87198589

Centisome position: 17.24

GC content: 64.09

Gene sequence:

>660_bases
ATGAGCACGCAAGACGGACAGAAGGCAGGCTGGAGCCGCGACCAGATGGCGGCGCGCGCCGCGAAGGAACTGCGCGACGG
CTACTACGTGAACCTCGGGATCGGCATTCCGACGCTGGTCGCCAACCACGTGCCCGAAGGCATGACCGTGACGCTCCAGT
CCGAAAACGGAATGCTGGGCATCGGTCCCTTCCCTTATGACGACGAGGTCGATCCGGACCTGATCAACGCCGGCAAGCAG
ACTATCAGCGAGCTGCCGCAGTCCGCCTATTTCGACAGCGCCACCAGCTTTGCGATGATCCGCGGCGGCAAGATCGACCT
CACCGTGCTGGGCGCGATGGAAGTGGCCGAGAACGGCGACATCGCGAACTGGATGGTGCCGGGCAAGATGATCAAGGGCA
TGGGCGGGGCGATGGACCTCGTCGCGGGCGTCAAGAAGATCATCGTGGTCATGGAACACGTCGCCAAGGACGGCAGCCCG
AAGTTCATTCCCACCTGCACCCTGCCGCTGACCGGGCGCAACGTCGTCGACATGATCGTGACGGACCTTGCAGTCTTCCA
GCGTCCGGACCACAACTCTCCCTTCCGCCTGGTCGAACTGGCACCGGGCGTCACCGCCGACGAGGTGCGCGCCAGAACGA
CGGCGCACTACGTCGAGTAA

Upstream 100 bases:

>100_bases
CCGGACGGGCCGGTCGCGCTGGCGCTGGAGCAGCACATCGGGGCCGGCTGGTTCCTGGTGCCGCCTCCGATCCGATGAAT
TCTGACAGGACAGGAAACCG

Downstream 100 bases:

>100_bases
CAAAGGGAGAAACTGCCTTGCCGGATACCCTCGCCGCCATACCGCTCACCCGCATCGACGGAACCGCGGACAGCCTCGCG
AACCATGCCGGCAAGGTGCT

Product: butyryl-CoA:acetate CoA transferase

Products: NA

Alternate protein names: OXCT B; Succinyl CoA:3-oxoacid CoA-transferase [H]

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MSTQDGQKAGWSRDQMAARAAKELRDGYYVNLGIGIPTLVANHVPEGMTVTLQSENGMLGIGPFPYDDEVDPDLINAGKQ
TISELPQSAYFDSATSFAMIRGGKIDLTVLGAMEVAENGDIANWMVPGKMIKGMGGAMDLVAGVKKIIVVMEHVAKDGSP
KFIPTCTLPLTGRNVVDMIVTDLAVFQRPDHNSPFRLVELAPGVTADEVRARTTAHYVE

Sequences:

>Translated_219_residues
MSTQDGQKAGWSRDQMAARAAKELRDGYYVNLGIGIPTLVANHVPEGMTVTLQSENGMLGIGPFPYDDEVDPDLINAGKQ
TISELPQSAYFDSATSFAMIRGGKIDLTVLGAMEVAENGDIANWMVPGKMIKGMGGAMDLVAGVKKIIVVMEHVAKDGSP
KFIPTCTLPLTGRNVVDMIVTDLAVFQRPDHNSPFRLVELAPGVTADEVRARTTAHYVE
>Mature_218_residues
STQDGQKAGWSRDQMAARAAKELRDGYYVNLGIGIPTLVANHVPEGMTVTLQSENGMLGIGPFPYDDEVDPDLINAGKQT
ISELPQSAYFDSATSFAMIRGGKIDLTVLGAMEVAENGDIANWMVPGKMIKGMGGAMDLVAGVKKIIVVMEHVAKDGSPK
FIPTCTLPLTGRNVVDMIVTDLAVFQRPDHNSPFRLVELAPGVTADEVRARTTAHYVE

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG2057

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit B family [H]

Homologues:

Organism=Homo sapiens, GI4557817, Length=206, Percent_Identity=53.8834951456311, Blast_Score=229, Evalue=1e-60,
Organism=Homo sapiens, GI11545841, Length=214, Percent_Identity=52.803738317757, Blast_Score=229, Evalue=2e-60,
Organism=Escherichia coli, GI1788552, Length=206, Percent_Identity=54.8543689320388, Blast_Score=226, Evalue=9e-61,
Organism=Caenorhabditis elegans, GI17531517, Length=210, Percent_Identity=52.3809523809524, Blast_Score=225, Evalue=2e-59,
Organism=Drosophila melanogaster, GI21358325, Length=206, Percent_Identity=57.2815533980583, Blast_Score=252, Evalue=1e-67,
Organism=Drosophila melanogaster, GI24655794, Length=206, Percent_Identity=57.2815533980583, Blast_Score=252, Evalue=1e-67,

Paralogues:

None

Copy number: 369 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012791
- InterPro:   IPR004165
- InterPro:   IPR004164 [H]

Pfam domain/function: PF01144 CoA_trans [H]

EC number: =2.8.3.5 [H]

Molecular weight: Translated: 23534; Mature: 23403

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTQDGQKAGWSRDQMAARAAKELRDGYYVNLGIGIPTLVANHVPEGMTVTLQSENGMLG
CCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHCCCCCEEEEECCCCEEE
IGPFPYDDEVDPDLINAGKQTISELPQSAYFDSATSFAMIRGGKIDLTVLGAMEVAENGD
CCCCCCCCCCCHHHHHHHHHHHHHCCHHHHCCCCCCEEEEECCEEEEEEEEEEHHHCCCC
IANWMVPGKMIKGMGGAMDLVAGVKKIIVVMEHVAKDGSPKFIPTCTLPLTGRNVVDMIV
CCEEECCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHH
TDLAVFQRPDHNSPFRLVELAPGVTADEVRARTTAHYVE
HHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
STQDGQKAGWSRDQMAARAAKELRDGYYVNLGIGIPTLVANHVPEGMTVTLQSENGMLG
CCCCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHCCCCCEEEEECCCCEEE
IGPFPYDDEVDPDLINAGKQTISELPQSAYFDSATSFAMIRGGKIDLTVLGAMEVAENGD
CCCCCCCCCCCHHHHHHHHHHHHHCCHHHHCCCCCCEEEEECCEEEEEEEEEEHHHCCCC
IANWMVPGKMIKGMGGAMDLVAGVKKIIVVMEHVAKDGSPKFIPTCTLPLTGRNVVDMIV
CCEEECCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHH
TDLAVFQRPDHNSPFRLVELAPGVTADEVRARTTAHYVE
HHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9385134 [H]