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The map label for this gene is citE [H]

Identifier: 87198118

GI number: 87198118

Start: 95267

End: 96148

Strand: Direct

Name: citE [H]

Synonym: Saro_0092

Alternate gene names: 87198118

Gene position: 95267-96148 (Clockwise)

Preceding gene: 87198117

Following gene: 87198121

Centisome position: 2.67

GC content: 67.12

Gene sequence:

>882_bases
ATGCCGCCCAGATCCTGGCTCTTCGTACCCGGAGACAGCGAGAAGAAGCTGGGCAAGGCCATGGCCACGGGCGCGCATGC
GGTAATCGTCGACCTCGAGGACGCGGTGGCGCCGGCGGCAAAGCCGCAGGCCCGCGTCCTCGCCCGGGACTGGCTTGGAA
TCCACCGCCAGCACGTGACCGAAAACCGCCCCGTGGCGCGGTGGGTGCGCATCAACGCAATCGACACCGGCATGTGGCGC
GAGGACCTGCAGGTCGTCATGGCTGGCGCGCCAGACGGCGTGATGCTGCCCAAGTGCGAGGGACCAGAACAGGTCCGCCA
GCTTGCCGCTGAAATCTATGAGCTGGAACAGCGCAACCGCGTGCCCAACGGTTCGACGCGGATCCTGCCTCTGGTCAGCG
AGACAGCCAAGTCGGCGCTGACCATCACCAGCTACGTCGACGAACCCATGCCGCGCCTAGCCGGGCTGACATGGGGCGCC
GAAGATCTTTCGGCCGTGCTGGGCGCCACGCGCAAGCGGGATGCCGCCGGCGCGTGGACAGACGCTTTCCGGTTCATCCG
CGCGCAATGCCTGCTGACCTCGCACGCGAAGGGCGTCTGGGCGATCGACACCCTGCACGACGATTTTCGCGACGAGGCGG
GCACGAAGCGGGCGGCCGAGGCCGCGCGAGCCGACGGATTCACCGGCATGCTGGCCATCCACCCATCGCAGGTGCCGATC
ATCAACACGGCCTTTGCTCCGACGGAGGCTGAACTTGCCGAAGCGGAAGCCATTGTCGCACTGTTCGCCGCCAGCCCCTA
TGCCGGGACGCTGCAATACAACGGCCGCATGGTGGACCAGCCGCACCTCAGGATGGCGAAGCAGCTACTCGGCAGAACCT
GA

Upstream 100 bases:

>100_bases
AGGGGGTGCCTGCAACGTGGTATTTCCCGGGCGAAGCAGGCTATACCGACTATCCGACACTGGCCGAGGAAACGGCCATA
GCCTGAAGGAGTGATGCCGC

Downstream 100 bases:

>100_bases
AGCAGGCGCCGACGCCGACGCCGACGCCGCCGGGGTTGCGGGAGTGGCGGCGGGCTCTTCCGCCGCTTCGCTCGCCGAGG
CGCCCTCCGCCTTGCGGCTA

Product: HpcH/HpaI aldolase

Products: acetate; oxaloacetate

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MPPRSWLFVPGDSEKKLGKAMATGAHAVIVDLEDAVAPAAKPQARVLARDWLGIHRQHVTENRPVARWVRINAIDTGMWR
EDLQVVMAGAPDGVMLPKCEGPEQVRQLAAEIYELEQRNRVPNGSTRILPLVSETAKSALTITSYVDEPMPRLAGLTWGA
EDLSAVLGATRKRDAAGAWTDAFRFIRAQCLLTSHAKGVWAIDTLHDDFRDEAGTKRAAEAARADGFTGMLAIHPSQVPI
INTAFAPTEAELAEAEAIVALFAASPYAGTLQYNGRMVDQPHLRMAKQLLGRT

Sequences:

>Translated_293_residues
MPPRSWLFVPGDSEKKLGKAMATGAHAVIVDLEDAVAPAAKPQARVLARDWLGIHRQHVTENRPVARWVRINAIDTGMWR
EDLQVVMAGAPDGVMLPKCEGPEQVRQLAAEIYELEQRNRVPNGSTRILPLVSETAKSALTITSYVDEPMPRLAGLTWGA
EDLSAVLGATRKRDAAGAWTDAFRFIRAQCLLTSHAKGVWAIDTLHDDFRDEAGTKRAAEAARADGFTGMLAIHPSQVPI
INTAFAPTEAELAEAEAIVALFAASPYAGTLQYNGRMVDQPHLRMAKQLLGRT
>Mature_292_residues
PPRSWLFVPGDSEKKLGKAMATGAHAVIVDLEDAVAPAAKPQARVLARDWLGIHRQHVTENRPVARWVRINAIDTGMWRE
DLQVVMAGAPDGVMLPKCEGPEQVRQLAAEIYELEQRNRVPNGSTRILPLVSETAKSALTITSYVDEPMPRLAGLTWGAE
DLSAVLGATRKRDAAGAWTDAFRFIRAQCLLTSHAKGVWAIDTLHDDFRDEAGTKRAAEAARADGFTGMLAIHPSQVPII
NTAFAPTEAELAEAEAIVALFAASPYAGTLQYNGRMVDQPHLRMAKQLLGRT

Specific function: May play a role in fatty acid biosynthesis (Potential) [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. Citrate lyase beta subunit-like subfamily [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=299, Percent_Identity=30.1003344481605, Blast_Score=139, Evalue=2e-33,
Organism=Escherichia coli, GI87081764, Length=293, Percent_Identity=31.740614334471, Blast_Score=111, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17557458, Length=302, Percent_Identity=28.1456953642384, Blast_Score=105, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.3.6

Molecular weight: Translated: 31917; Mature: 31786

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPRSWLFVPGDSEKKLGKAMATGAHAVIVDLEDAVAPAAKPQARVLARDWLGIHRQHVT
CCCCCEEEECCCCHHHHHHHHHCCCEEEEEECHHHHCCCCCCHHHHHHHHHHHHHHHHHC
ENRPVARWVRINAIDTGMWREDLQVVMAGAPDGVMLPKCEGPEQVRQLAAEIYELEQRNR
CCCCEEEEEEEEEECCCCCHHHHHEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
VPNGSTRILPLVSETAKSALTITSYVDEPMPRLAGLTWGAEDLSAVLGATRKRDAAGAWT
CCCCCCEEEEHHHHHHHHHEEHHHHHCCHHHHHHCCCCCHHHHHHHHCCCCCCCCCCHHH
DAFRFIRAQCLLTSHAKGVWAIDTLHDDFRDEAGTKRAAEAARADGFTGMLAIHPSQVPI
HHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEECCCCCCE
INTAFAPTEAELAEAEAIVALFAASPYAGTLQYNGRMVDQPHLRMAKQLLGRT
EEECCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCEECCCHHHHHHHHHHCCC
>Mature Secondary Structure 
PPRSWLFVPGDSEKKLGKAMATGAHAVIVDLEDAVAPAAKPQARVLARDWLGIHRQHVT
CCCCEEEECCCCHHHHHHHHHCCCEEEEEECHHHHCCCCCCHHHHHHHHHHHHHHHHHC
ENRPVARWVRINAIDTGMWREDLQVVMAGAPDGVMLPKCEGPEQVRQLAAEIYELEQRNR
CCCCEEEEEEEEEECCCCCHHHHHEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
VPNGSTRILPLVSETAKSALTITSYVDEPMPRLAGLTWGAEDLSAVLGATRKRDAAGAWT
CCCCCCEEEEHHHHHHHHHEEHHHHHCCHHHHHHCCCCCHHHHHHHHCCCCCCCCCCHHH
DAFRFIRAQCLLTSHAKGVWAIDTLHDDFRDEAGTKRAAEAARADGFTGMLAIHPSQVPI
HHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEECCCCCCE
INTAFAPTEAELAEAEAIVALFAASPYAGTLQYNGRMVDQPHLRMAKQLLGRT
EEECCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCEECCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.345 {acetyl-CoA}} 0.0718 {acetyl-CoA}} 0.0367 {oxaloacetate}} 0.0565 {oxaoacetate}} [C]

Substrates: citrate

Specific reaction: citrate = acetate + oxaloacetate

General reaction: elimination (of an oxo-acid C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]