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The map label for this gene is nemA [H]

Identifier: 87198117

GI number: 87198117

Start: 94149

End: 95252

Strand: Direct

Name: nemA [H]

Synonym: Saro_0091

Alternate gene names: 87198117

Gene position: 94149-95252 (Clockwise)

Preceding gene: 87198115

Following gene: 87198118

Centisome position: 2.64

GC content: 66.21

Gene sequence:

>1104_bases
ATGCATGAACCGCTGTTCCAGCCTGTGCGCCTCGGCGCCATCGAAGCGCCCAATCGCATCATCATGGCCCCGCTCACCCG
TGGCCGCGCGAATCCCCAGGCGGTGCCGACACCGATCATGGGCGAATATTATCGCCAGCGCGCCAGCGCCGGCCTGATCA
TCTCCGAGGCGACCGCGATCAGCCCCGAGGGCGCCGGGTGGCCGAGCGCGCCGGGCATCTGGACCGACGAGCAGACCGAG
GCGTGGAAGCCGATAGTCGATGAAGTTCATGCGGCGGGCGGACGCATCGTCCTGCAGCTTTGGCACATGGGCCGCGTGGT
CCACCCCTATTTCCTCGGCGGCGAGCCACCTGTTTCGGCATCGGCGACGCGTGCACCCGGCCATGCCCATACGCCGGAAG
GCACCAAGGACTACGAGACAGCGCGCCCCCTGCGGCTGGACGAGATTCCCCGTCTGATCGACGATTATGGCCGGGCGGCT
GAAAATGCACGGAAGGCCGGGTTCGACGGGGTGCAGCTCCACGCCGCGAACGGCTACCTTATCGACCAGTTCCTGCGCGA
CGGCACCAACCTTCGCGACGACGATTACGGCGGCAGCCCCGAGAACCGGGTGCGCCTGATGCGCGAGGTGATGGAGCGGC
TGATCTCGATCTGGGGCAAGGAGCGGGTGTCGATCCGCCTTTCGCCCAACGGCGATTCGCAAGGGGTCGACGATAGCAAC
CCCGAGGCAACCTTTGGCGAAGCCGCGCGAGTGCTCGAGGAACTGGGCGTCGGCTTCGTCGAACTTCGCCAGCCGGGACC
GGAAGGCACATTCGGCAACACTGACGTGCCCAAGCAGGACGGCCTGATCCGCTCGATCTACACCGGACCGCTTGTGCTCA
ACAGCGACTACACGCCGGAGGAGGCTGCCGCAGACGTGGCGTCCGGCCGCGCGGACGCGATAAGCTTTGGCCGCCCGTTC
ATCTCCAATCCCGATCTCGTCGAGCGCATCCGCGTGGGCGCGCACCTCAATCCGAACAAGGGGGTGCCTGCAACGTGGTA
TTTCCCGGGCGAAGCAGGCTATACCGACTATCCGACACTGGCCGAGGAAACGGCCATAGCCTGA

Upstream 100 bases:

>100_bases
TCACTTGAATTCGTCATGGGGGCCGCTTAATCCAGTTGCAAAGCGAAACCGAGCTCTTTCATCGCGCGGTGTCGCCGAAG
TCATCAAGGAGCCCCCTAGC

Downstream 100 bases:

>100_bases
AGGAGTGATGCCGCATGCCGCCCAGATCCTGGCTCTTCGTACCCGGAGACAGCGAGAAGAAGCTGGGCAAGGCCATGGCC
ACGGGCGCGCATGCGGTAAT

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MHEPLFQPVRLGAIEAPNRIIMAPLTRGRANPQAVPTPIMGEYYRQRASAGLIISEATAISPEGAGWPSAPGIWTDEQTE
AWKPIVDEVHAAGGRIVLQLWHMGRVVHPYFLGGEPPVSASATRAPGHAHTPEGTKDYETARPLRLDEIPRLIDDYGRAA
ENARKAGFDGVQLHAANGYLIDQFLRDGTNLRDDDYGGSPENRVRLMREVMERLISIWGKERVSIRLSPNGDSQGVDDSN
PEATFGEAARVLEELGVGFVELRQPGPEGTFGNTDVPKQDGLIRSIYTGPLVLNSDYTPEEAAADVASGRADAISFGRPF
ISNPDLVERIRVGAHLNPNKGVPATWYFPGEAGYTDYPTLAEETAIA

Sequences:

>Translated_367_residues
MHEPLFQPVRLGAIEAPNRIIMAPLTRGRANPQAVPTPIMGEYYRQRASAGLIISEATAISPEGAGWPSAPGIWTDEQTE
AWKPIVDEVHAAGGRIVLQLWHMGRVVHPYFLGGEPPVSASATRAPGHAHTPEGTKDYETARPLRLDEIPRLIDDYGRAA
ENARKAGFDGVQLHAANGYLIDQFLRDGTNLRDDDYGGSPENRVRLMREVMERLISIWGKERVSIRLSPNGDSQGVDDSN
PEATFGEAARVLEELGVGFVELRQPGPEGTFGNTDVPKQDGLIRSIYTGPLVLNSDYTPEEAAADVASGRADAISFGRPF
ISNPDLVERIRVGAHLNPNKGVPATWYFPGEAGYTDYPTLAEETAIA
>Mature_367_residues
MHEPLFQPVRLGAIEAPNRIIMAPLTRGRANPQAVPTPIMGEYYRQRASAGLIISEATAISPEGAGWPSAPGIWTDEQTE
AWKPIVDEVHAAGGRIVLQLWHMGRVVHPYFLGGEPPVSASATRAPGHAHTPEGTKDYETARPLRLDEIPRLIDDYGRAA
ENARKAGFDGVQLHAANGYLIDQFLRDGTNLRDDDYGGSPENRVRLMREVMERLISIWGKERVSIRLSPNGDSQGVDDSN
PEATFGEAARVLEELGVGFVELRQPGPEGTFGNTDVPKQDGLIRSIYTGPLVLNSDYTPEEAAADVASGRADAISFGRPF
ISNPDLVERIRVGAHLNPNKGVPATWYFPGEAGYTDYPTLAEETAIA

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=370, Percent_Identity=45.4054054054054, Blast_Score=287, Evalue=9e-79,
Organism=Escherichia coli, GI1789463, Length=346, Percent_Identity=30.0578034682081, Blast_Score=119, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17565138, Length=358, Percent_Identity=26.8156424581006, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17559804, Length=361, Percent_Identity=26.0387811634349, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17564188, Length=353, Percent_Identity=25.7790368271955, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI72001454, Length=355, Percent_Identity=26.4788732394366, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17559802, Length=353, Percent_Identity=26.3456090651558, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17540738, Length=226, Percent_Identity=28.3185840707965, Blast_Score=74, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6325086, Length=378, Percent_Identity=37.3015873015873, Blast_Score=202, Evalue=7e-53,
Organism=Saccharomyces cerevisiae, GI6321973, Length=378, Percent_Identity=35.7142857142857, Blast_Score=190, Evalue=3e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 39922; Mature: 39922

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHEPLFQPVRLGAIEAPNRIIMAPLTRGRANPQAVPTPIMGEYYRQRASAGLIISEATAI
CCCCCCCHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCC
SPEGAGWPSAPGIWTDEQTEAWKPIVDEVHAAGGRIVLQLWHMGRVVHPYFLGGEPPVSA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHCCCCCCEEECCCCCCCC
SATRAPGHAHTPEGTKDYETARPLRLDEIPRLIDDYGRAAENARKAGFDGVQLHAANGYL
CCCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHH
IDQFLRDGTNLRDDDYGGSPENRVRLMREVMERLISIWGKERVSIRLSPNGDSQGVDDSN
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCC
PEATFGEAARVLEELGVGFVELRQPGPEGTFGNTDVPKQDGLIRSIYTGPLVLNSDYTPE
CCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCEEECCCCCCH
EAAADVASGRADAISFGRPFISNPDLVERIRVGAHLNPNKGVPATWYFPGEAGYTDYPTL
HHHHHHHCCCHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCH
AEETAIA
HHHHCCC
>Mature Secondary Structure
MHEPLFQPVRLGAIEAPNRIIMAPLTRGRANPQAVPTPIMGEYYRQRASAGLIISEATAI
CCCCCCCHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEECCCCC
SPEGAGWPSAPGIWTDEQTEAWKPIVDEVHAAGGRIVLQLWHMGRVVHPYFLGGEPPVSA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHCCCCCCEEECCCCCCCC
SATRAPGHAHTPEGTKDYETARPLRLDEIPRLIDDYGRAAENARKAGFDGVQLHAANGYL
CCCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHH
IDQFLRDGTNLRDDDYGGSPENRVRLMREVMERLISIWGKERVSIRLSPNGDSQGVDDSN
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCC
PEATFGEAARVLEELGVGFVELRQPGPEGTFGNTDVPKQDGLIRSIYTGPLVLNSDYTPE
CCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCEEECCCCCCH
EAAADVASGRADAISFGRPFISNPDLVERIRVGAHLNPNKGVPATWYFPGEAGYTDYPTL
HHHHHHHCCCHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCH
AEETAIA
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]