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The map label for this gene is yhcR [H]

Identifier: 86751555

GI number: 86751555

Start: 5045112

End: 5046791

Strand: Direct

Name: yhcR [H]

Synonym: RPB_4457

Alternate gene names: 86751555

Gene position: 5045112-5046791 (Clockwise)

Preceding gene: 86751553

Following gene: 86751556

Centisome position: 94.63

GC content: 69.23

Gene sequence:

>1680_bases
ATGATGACCCTTTTGAAACGTCGCCTCGCCCTGCTCGCGCCCGTATTGGCGCTGGCGCTGGCCTCGACCGCGCTCCGCGC
CGACGATGCGGCGACGTCGGTCGAGCTGCGGATTCTCGCGATCAATGATTTCCACGGCTATCTGCAGCCGCCGCCGGGCG
GCCTGGCGCTGCCCGACCCGGCCGATCCGGACAAGAAGACCAGCGTGCCGGCCGGCGGCGCCGAGCACATGGCGACGCTG
GTCCAACAGTTGCGCGCGGGACACGCCCACAGCATCTTCGTCGCCGCCGGCGACCTGATCGGCGCCAGCCCGTTTTTGTC
GGCGATGTTTCACGACGAGCCGACGATCGAATCGATGTCGCTGATGGGGCTGGCGCTGTCGGCGGTCGGCAATCACGAAT
TCGACGAGGGCCGGACCGAGCTGCTGCGGATGCAGCACGGCGGCTGCCATCCGGTCGACGGCTGCCTCGGGCCGCATCCG
TTTACCGGGGCGAAATTCCAGTATCTCGCCGCCTCGACGGTGGATACCGCGACCGGCAAGACCCTGCTGCCGGCGACCGC
GGTGCGTGACTTCGGCGGCATCCCGGTCGGCTTCATCGGCCTGACGCTGAAGACGACGCCGACCATGGTGTCGCCGCCGG
GCGTCGCCGGGCTGCAGTTCAGGGACGAGGCCGAGACCGTCAACGAACAGGTCGCCGAGCTGAAGGCCCGCGGCGTCGAG
GCCATCGTGGTGCTGATCCACGAAGGCGGCTTTCCGACCGGCGGCATCAACGACTGTCCGGGAATCTCCGGGCCGATCGT
CGAGATTGTCAGGAAGTTCGACAGGGCCGTCGACCTGGTGATCAGCGGCCACACCCACCGCGCCTACACCTGCACGATCG
ACGGCCGGCTGGTCACCAGCGGCGACCGGTACGGCACGCTGGTCACCGCGATCGACCTCGTGCTCGATCCCAAGACCCGC
GACGTCGTCAGCGCCAGGGCTGATAATGTCGTTGTGCGCACCGAGACGCTGGCGAAGGACCCGGCGCAGACCGCGCTGAT
CGACAGCTACGACAGGCTCGCCGGGCCGATCGCGGCGCGGCCCGCCGGCAGCGTCACCGCAGCGGTGTCGCGGATGCCGA
ATGCGGCCGGCGAAAGCGCGCTCGGCGATCTGGTCGCCGACGCTCATCTCGCCGCCACGCGCGACCGCGAGACCGGCGGC
GCCGAGATCGCGATCACCAATCCCGGCGGGCTGCGCGCCGATATCAACGCCGCGGAAGATGGAAAGGTGACGTTCGGCGA
TGTCTTCGCCGCGCAGCCGTTCCGCAATCAGCTGGTGACGATGACGCTGACCGGCGCGCAGCTCAAAGCGGCGCTGGAGC
TGCAATGGCAGGATCCGGCGCGTCCGCGCATCCTGCAGGTGTCGCGCGGCTTCAGCTATGCGTGGGACAATACCGCCGCA
CCGGGCGCGCGCATCGTCGCCGAGCGGATGCTGCTGCACGGCAAGCCGATCACGGCCGAGCGCAGCTACCGCGTCACGAT
CAACGCCTATCTGGCCGCCGGCGGCGACGGCTTCACGCCGTTCACGCAGGGCACCGACCGCCTGACTGGCGTCTACGACG
TCGACGCCCTGTTCGGCTATTTCCGGGCGCACAGCCCGATCGCGCCGGCGCCGCTGGACCGGATCTCGCGCATGAACTGA

Upstream 100 bases:

>100_bases
GCGCTTGGCCGCGCCGATGGACGGCGCCGACGCGCCACAAACAGGTTGCCAGCGCGCCCCGCCTGAGCGATGCCCGCGCC
GAGCGTAGCACACCGGACAC

Downstream 100 bases:

>100_bases
GCGGCCGCGCGCGTATCACGTAAACGTGGTGAACGTATTGCCACCGCGCGCGCTGCTGATCGAATGCAGCATTTTCTTGC
GCCGGACCGTTTCCACTTTG

Product: 5'-nucleotidase-like protein

Products: ribonucleoside; phosphate

Alternate protein names: NA

Number of amino acids: Translated: 559; Mature: 559

Protein sequence:

>559_residues
MMTLLKRRLALLAPVLALALASTALRADDAATSVELRILAINDFHGYLQPPPGGLALPDPADPDKKTSVPAGGAEHMATL
VQQLRAGHAHSIFVAAGDLIGASPFLSAMFHDEPTIESMSLMGLALSAVGNHEFDEGRTELLRMQHGGCHPVDGCLGPHP
FTGAKFQYLAASTVDTATGKTLLPATAVRDFGGIPVGFIGLTLKTTPTMVSPPGVAGLQFRDEAETVNEQVAELKARGVE
AIVVLIHEGGFPTGGINDCPGISGPIVEIVRKFDRAVDLVISGHTHRAYTCTIDGRLVTSGDRYGTLVTAIDLVLDPKTR
DVVSARADNVVVRTETLAKDPAQTALIDSYDRLAGPIAARPAGSVTAAVSRMPNAAGESALGDLVADAHLAATRDRETGG
AEIAITNPGGLRADINAAEDGKVTFGDVFAAQPFRNQLVTMTLTGAQLKAALELQWQDPARPRILQVSRGFSYAWDNTAA
PGARIVAERMLLHGKPITAERSYRVTINAYLAAGGDGFTPFTQGTDRLTGVYDVDALFGYFRAHSPIAPAPLDRISRMN

Sequences:

>Translated_559_residues
MMTLLKRRLALLAPVLALALASTALRADDAATSVELRILAINDFHGYLQPPPGGLALPDPADPDKKTSVPAGGAEHMATL
VQQLRAGHAHSIFVAAGDLIGASPFLSAMFHDEPTIESMSLMGLALSAVGNHEFDEGRTELLRMQHGGCHPVDGCLGPHP
FTGAKFQYLAASTVDTATGKTLLPATAVRDFGGIPVGFIGLTLKTTPTMVSPPGVAGLQFRDEAETVNEQVAELKARGVE
AIVVLIHEGGFPTGGINDCPGISGPIVEIVRKFDRAVDLVISGHTHRAYTCTIDGRLVTSGDRYGTLVTAIDLVLDPKTR
DVVSARADNVVVRTETLAKDPAQTALIDSYDRLAGPIAARPAGSVTAAVSRMPNAAGESALGDLVADAHLAATRDRETGG
AEIAITNPGGLRADINAAEDGKVTFGDVFAAQPFRNQLVTMTLTGAQLKAALELQWQDPARPRILQVSRGFSYAWDNTAA
PGARIVAERMLLHGKPITAERSYRVTINAYLAAGGDGFTPFTQGTDRLTGVYDVDALFGYFRAHSPIAPAPLDRISRMN
>Mature_559_residues
MMTLLKRRLALLAPVLALALASTALRADDAATSVELRILAINDFHGYLQPPPGGLALPDPADPDKKTSVPAGGAEHMATL
VQQLRAGHAHSIFVAAGDLIGASPFLSAMFHDEPTIESMSLMGLALSAVGNHEFDEGRTELLRMQHGGCHPVDGCLGPHP
FTGAKFQYLAASTVDTATGKTLLPATAVRDFGGIPVGFIGLTLKTTPTMVSPPGVAGLQFRDEAETVNEQVAELKARGVE
AIVVLIHEGGFPTGGINDCPGISGPIVEIVRKFDRAVDLVISGHTHRAYTCTIDGRLVTSGDRYGTLVTAIDLVLDPKTR
DVVSARADNVVVRTETLAKDPAQTALIDSYDRLAGPIAARPAGSVTAAVSRMPNAAGESALGDLVADAHLAATRDRETGG
AEIAITNPGGLRADINAAEDGKVTFGDVFAAQPFRNQLVTMTLTGAQLKAALELQWQDPARPRILQVSRGFSYAWDNTAA
PGARIVAERMLLHGKPITAERSYRVTINAYLAAGGDGFTPFTQGTDRLTGVYDVDALFGYFRAHSPIAPAPLDRISRMN

Specific function: Sugar-nonspecific endonuclease that yields nucleotide 3'-monophosphate products. No 5'-nucleotidase activity was detected, using 5'-AMP as the substrate, in the presence of diverse divalent metals and with various pH values [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TNase-like domain [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=565, Percent_Identity=24.6017699115044, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1786687, Length=595, Percent_Identity=22.5210084033613, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1790658, Length=246, Percent_Identity=25.2032520325203, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24652512, Length=469, Percent_Identity=25.5863539445629, Blast_Score=115, Evalue=1e-25,
Organism=Drosophila melanogaster, GI19921980, Length=469, Percent_Identity=25.5863539445629, Blast_Score=115, Evalue=1e-25,
Organism=Drosophila melanogaster, GI161076508, Length=469, Percent_Identity=25.5863539445629, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24641187, Length=579, Percent_Identity=23.4887737478411, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI221329836, Length=571, Percent_Identity=24.6935201401051, Blast_Score=109, Evalue=6e-24,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019948
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR016027
- InterPro:   IPR004365
- InterPro:   IPR016071
- InterPro:   IPR006021 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00746 Gram_pos_anchor; PF00149 Metallophos; PF00565 SNase; PF01336 tRNA_anti [H]

EC number: 3.1.3.5

Molecular weight: Translated: 58893; Mature: 58893

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00785 5_NUCLEOTIDASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTLLKRRLALLAPVLALALASTALRADDAATSVELRILAINDFHGYLQPPPGGLALPDP
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCEECCCCCCCCCCCC
ADPDKKTSVPAGGAEHMATLVQQLRAGHAHSIFVAAGDLIGASPFLSAMFHDEPTIESMS
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHCCCCCHHHHH
LMGLALSAVGNHEFDEGRTELLRMQHGGCHPVDGCLGPHPFTGAKFQYLAASTVDTATGK
HHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCC
TLLPATAVRDFGGIPVGFIGLTLKTTPTMVSPPGVAGLQFRDEAETVNEQVAELKARGVE
EECCHHHHHHCCCCCEEEEEEEEECCCCEECCCCCCCCEECCHHHHHHHHHHHHHHCCCE
AIVVLIHEGGFPTGGINDCPGISGPIVEIVRKFDRAVDLVISGHTHRAYTCTIDGRLVTS
EEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHEEEEEECCCCEEEEEEECCEEEEC
GDRYGTLVTAIDLVLDPKTRDVVSARADNVVVRTETLAKDPAQTALIDSYDRLAGPIAAR
CCCCCHHEEEEEHEECCCHHHHHHHCCCCEEEEEHHHHCCCHHHHHHHHHHHHCCCCCCC
PAGSVTAAVSRMPNAAGESALGDLVADAHLAATRDRETGGAEIAITNPGGLRADINAAED
CCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEEECCCCCC
GKVTFGDVFAAQPFRNQLVTMTLTGAQLKAALELQWQDPARPRILQVSRGFSYAWDNTAA
CCEEECHHECCCCHHCCEEEEEEECCEEEEEEEEEECCCCCCCEEEEECCCCCCCCCCCC
PGARIVAERMLLHGKPITAERSYRVTINAYLAAGGDGFTPFTQGTDRLTGVYDVDALFGY
CHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCCCEEEHHHHHHH
FRAHSPIAPAPLDRISRMN
HHCCCCCCCCCHHHHHCCC
>Mature Secondary Structure
MMTLLKRRLALLAPVLALALASTALRADDAATSVELRILAINDFHGYLQPPPGGLALPDP
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCEECCCCCCCCCCCC
ADPDKKTSVPAGGAEHMATLVQQLRAGHAHSIFVAAGDLIGASPFLSAMFHDEPTIESMS
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHCCCCCHHHHH
LMGLALSAVGNHEFDEGRTELLRMQHGGCHPVDGCLGPHPFTGAKFQYLAASTVDTATGK
HHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCC
TLLPATAVRDFGGIPVGFIGLTLKTTPTMVSPPGVAGLQFRDEAETVNEQVAELKARGVE
EECCHHHHHHCCCCCEEEEEEEEECCCCEECCCCCCCCEECCHHHHHHHHHHHHHHCCCE
AIVVLIHEGGFPTGGINDCPGISGPIVEIVRKFDRAVDLVISGHTHRAYTCTIDGRLVTS
EEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHEEEEEECCCCEEEEEEECCEEEEC
GDRYGTLVTAIDLVLDPKTRDVVSARADNVVVRTETLAKDPAQTALIDSYDRLAGPIAAR
CCCCCHHEEEEEHEECCCHHHHHHHCCCCEEEEEHHHHCCCHHHHHHHHHHHHCCCCCCC
PAGSVTAAVSRMPNAAGESALGDLVADAHLAATRDRETGGAEIAITNPGGLRADINAAED
CCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEEECCCCCC
GKVTFGDVFAAQPFRNQLVTMTLTGAQLKAALELQWQDPARPRILQVSRGFSYAWDNTAA
CCEEECHHECCCCHHCCEEEEEEECCEEEEEEEEEECCCCCCCEEEEECCCCCCCCCCCC
PGARIVAERMLLHGKPITAERSYRVTINAYLAAGGDGFTPFTQGTDRLTGVYDVDALFGY
CHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCCCEEEHHHHHHH
FRAHSPIAPAPLDRISRMN
HHCCCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 5'-ribonucleotide; H2O

Specific reaction: A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969498; 9384377 [H]