Click here to switch to the map view.

The map label for this gene is nreC [H]

Identifier: 86751547

GI number: 86751547

Start: 5033940

End: 5034632

Strand: Direct

Name: nreC [H]

Synonym: RPB_4449

Alternate gene names: 86751547

Gene position: 5033940-5034632 (Clockwise)

Preceding gene: 86751540

Following gene: 86751548

Centisome position: 94.42

GC content: 63.92

Gene sequence:

>693_bases
ATGGACGCGAAATCACCGAACCCACCCCATGGCTCACGTTTGCGGATCTTTCTCGCCGACGATCATCCGATTGTTCTGAG
TGGGATGAAGATGCTCGTCGCCGAAGATCCCGAACTCGAGCTGGTCGGCGAGGCCAATGACGGACCCAAGGCGCTGCGGC
GCGCCATCGAGCTGAGGCCCGACGTCGCGGTGTTCGATCTGTCGATGCCGGGCATGAACGGAATTGACGTCACCGAGAAG
TACCTCGAGGCGCAGCCGAAGGCGCGCGTGCTCGTCCTATCGGTGCACGAGGATGGTGCTTATCTGCGGCGGCTGCTCAA
AATGGGCGTCGGCGGCTACATCCTCAAGCGCTCGGCGACCGACGAACTGATCCGCGGCATCCACGCCGTCGCAGCCGGCG
GGTTGTATCTCGATCCGGCGATCGCGGGGCGCGCCATCGGTCGGGGCGTCCAGCAGGTGTCCATCGACAGCGAAACCAGC
CCGATCTCGGAGCTCAGCACCCGCGAGCTGGAAGTCCTGCGGCTGGCCTCTGTCGGCCACAGCAACAAGGTGATCTCCGC
CAAACTCCAGGTCGGTTCCAAGAGCGTCGAGACCTACAAGGCGCGCGCCATGGACAAACTCGGTTTCACCAACCGGGTCG
AGTTGATCCGCTTCGCTCTCAGCATGGGCTGGCTGGAGAGCGCGGAGCCGTGA

Upstream 100 bases:

>100_bases
GCCCTACAGCATCGGAACGCGGGTCCTGCAATGCAGTCGGCCGAAATCGTGCGGTCGCTCGAGGAGGCAGCCAAGCTGTG
TGGGTTTGGCTGTGTGAACG

Downstream 100 bases:

>100_bases
CGGTGGCTCCTGCGCGATAGTCTGTCCCCGGACCTCGGGCTCCTGCAACCGATGCCGACCCTTGATCTATCGCAACATCG
ATGGGAGCCATCGGCGCAAG

Product: two component LuxR family transcriptional regulator

Products: NA

Alternate protein names: Nitrogen regulation protein C [H]

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MDAKSPNPPHGSRLRIFLADDHPIVLSGMKMLVAEDPELELVGEANDGPKALRRAIELRPDVAVFDLSMPGMNGIDVTEK
YLEAQPKARVLVLSVHEDGAYLRRLLKMGVGGYILKRSATDELIRGIHAVAAGGLYLDPAIAGRAIGRGVQQVSIDSETS
PISELSTRELEVLRLASVGHSNKVISAKLQVGSKSVETYKARAMDKLGFTNRVELIRFALSMGWLESAEP

Sequences:

>Translated_230_residues
MDAKSPNPPHGSRLRIFLADDHPIVLSGMKMLVAEDPELELVGEANDGPKALRRAIELRPDVAVFDLSMPGMNGIDVTEK
YLEAQPKARVLVLSVHEDGAYLRRLLKMGVGGYILKRSATDELIRGIHAVAAGGLYLDPAIAGRAIGRGVQQVSIDSETS
PISELSTRELEVLRLASVGHSNKVISAKLQVGSKSVETYKARAMDKLGFTNRVELIRFALSMGWLESAEP
>Mature_230_residues
MDAKSPNPPHGSRLRIFLADDHPIVLSGMKMLVAEDPELELVGEANDGPKALRRAIELRPDVAVFDLSMPGMNGIDVTEK
YLEAQPKARVLVLSVHEDGAYLRRLLKMGVGGYILKRSATDELIRGIHAVAAGGLYLDPAIAGRAIGRGVQQVSIDSETS
PISELSTRELEVLRLASVGHSNKVISAKLQVGSKSVETYKARAMDKLGFTNRVELIRFALSMGWLESAEP

Specific function: Member of the two-component regulatory system nreB/nreC involved in the control of dissimilatory nitrate/nitrite reduction in response to oxygen. Phosphorylated nreC binds to a GC-rich palindromic sequence at the promoters of the nitrate (narGHJI) and nit

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=210, Percent_Identity=28.5714285714286, Blast_Score=110, Evalue=8e-26,
Organism=Escherichia coli, GI1788712, Length=202, Percent_Identity=29.2079207920792, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1790102, Length=212, Percent_Identity=34.9056603773585, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1788521, Length=205, Percent_Identity=30.7317073170732, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787473, Length=199, Percent_Identity=31.6582914572864, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1786747, Length=205, Percent_Identity=26.3414634146341, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1788546, Length=210, Percent_Identity=28.0952380952381, Blast_Score=81, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 24950; Mature: 24950

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAKSPNPPHGSRLRIFLADDHPIVLSGMKMLVAEDPELELVGEANDGPKALRRAIELRP
CCCCCCCCCCCCEEEEEEECCCCEEEECCEEEEECCCCEEEEECCCCCHHHHHHHHHCCC
DVAVFDLSMPGMNGIDVTEKYLEAQPKARVLVLSVHEDGAYLRRLLKMGVGGYILKRSAT
CEEEEEECCCCCCCCHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHCCCCEEEECCCH
DELIRGIHAVAAGGLYLDPAIAGRAIGRGVQQVSIDSETSPISELSTRELEVLRLASVGH
HHHHHHHHHHHCCCEEECHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCC
SNKVISAKLQVGSKSVETYKARAMDKLGFTNRVELIRFALSMGWLESAEP
CCCEEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDAKSPNPPHGSRLRIFLADDHPIVLSGMKMLVAEDPELELVGEANDGPKALRRAIELRP
CCCCCCCCCCCCEEEEEEECCCCEEEECCEEEEECCCCEEEEECCCCCHHHHHHHHHCCC
DVAVFDLSMPGMNGIDVTEKYLEAQPKARVLVLSVHEDGAYLRRLLKMGVGGYILKRSAT
CEEEEEECCCCCCCCHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHCCCCEEEECCCH
DELIRGIHAVAAGGLYLDPAIAGRAIGRGVQQVSIDSETSPISELSTRELEVLRLASVGH
HHHHHHHHHHHCCCEEECHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCC
SNKVISAKLQVGSKSVETYKARAMDKLGFTNRVELIRFALSMGWLESAEP
CCCEEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA