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The map label for this gene is cofE [H]

Identifier: 86751509

GI number: 86751509

Start: 4995944

End: 4996702

Strand: Reverse

Name: cofE [H]

Synonym: RPB_4408

Alternate gene names: 86751509

Gene position: 4996702-4995944 (Counterclockwise)

Preceding gene: 86751510

Following gene: 86751508

Centisome position: 93.72

GC content: 68.38

Gene sequence:

>759_bases
TTGTCGGTCCGACGTGCTTTGGAGATATTTGCCCTGGAGGGCGTGCCGCTGGTCGAGCCCGGTGACGACCTGGCCGGTCT
GATCCTGGCATCGCTGGCGGATTGCGGCCGGCACCTGCAGGATCACGACATCGTCGTGATCGCGCAGAAAGTGGTCTCGA
AGGCCGAGGGCCGCGGCGTCGATCTGGCGGCGGTGACGCCGTCGCCGCGCGCTGTCGAGATCGCCGCGCAGGCCCACAAG
GATCCGCGGATCGTCGAATTGATCCTCGCGGAATCGAGCGAGGTGCTGCGCGTTCGGCCGGGCGTGATCATCGTCGAGCA
CCGGCTCGGCCTGGTGCTCGCCAATGCCGGCATCGATCGCTCCAATGTAGAGGGCGAAACCGCGCTGCTGCTGCCGCTCG
ATCCCGACGGTTCGGCGCAGCAGATCCGGGAGCAGCTGATCGCCGGGACCGGCGCGACCGTCGGCGTGATGATCATCGAC
AGCATCGGTCGCGCCTGGCGCAACGGCACCATCGGCACCACGATCGGCGCCAGCGGCATCGCCACGATGCGCGATCTGCG
TGGTCAGCCCGATTTGTTCGGCCGCAAGCTCGAGACCACCGAGGTCGGCGCCGCCGACGAAGTCGCGGCAGCGGCGTCGC
TGCTGATGGGGCAGGCCGCGGAGGCGACGCCGGTCGTCGTGGTCCGCGGGGTCGATCTCGCCGGCATCGGCACCGCGCGT
GATCTGCTGCGGCCCAAACACATGGATTTGTTTCGATGA

Upstream 100 bases:

>100_bases
GTGTCGGAGAGCCTCACTTCGGCGCTGATCTGGATCAATAAATTCTATAAGATTCCCGACGCGGGAGTGCGGATCACCGG
CCTCCCCTGAGGAGAGTCGA

Downstream 100 bases:

>100_bases
CCGCGCCTGTTGCAGACGATCACATCATTGCACTCGCCGGCGGCGTCGGCGGCGCCAAGCTGGCGGACGGTCTGGCGCAT
GCGCTCGGCGACAAGCTCAC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MSVRRALEIFALEGVPLVEPGDDLAGLILASLADCGRHLQDHDIVVIAQKVVSKAEGRGVDLAAVTPSPRAVEIAAQAHK
DPRIVELILAESSEVLRVRPGVIIVEHRLGLVLANAGIDRSNVEGETALLLPLDPDGSAQQIREQLIAGTGATVGVMIID
SIGRAWRNGTIGTTIGASGIATMRDLRGQPDLFGRKLETTEVGAADEVAAAASLLMGQAAEATPVVVVRGVDLAGIGTAR
DLLRPKHMDLFR

Sequences:

>Translated_252_residues
MSVRRALEIFALEGVPLVEPGDDLAGLILASLADCGRHLQDHDIVVIAQKVVSKAEGRGVDLAAVTPSPRAVEIAAQAHK
DPRIVELILAESSEVLRVRPGVIIVEHRLGLVLANAGIDRSNVEGETALLLPLDPDGSAQQIREQLIAGTGATVGVMIID
SIGRAWRNGTIGTTIGASGIATMRDLRGQPDLFGRKLETTEVGAADEVAAAASLLMGQAAEATPVVVVRGVDLAGIGTAR
DLLRPKHMDLFR
>Mature_251_residues
SVRRALEIFALEGVPLVEPGDDLAGLILASLADCGRHLQDHDIVVIAQKVVSKAEGRGVDLAAVTPSPRAVEIAAQAHKD
PRIVELILAESSEVLRVRPGVIIVEHRLGLVLANAGIDRSNVEGETALLLPLDPDGSAQQIREQLIAGTGATVGVMIIDS
IGRAWRNGTIGTTIGASGIATMRDLRGQPDLFGRKLETTEVGAADEVAAAASLLMGQAAEATPVVVVRGVDLAGIGTARD
LLRPKHMDLFR

Specific function: Catalyzes the GTP-dependent successive addition of multiple gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form polyglutamated F420 derivatives [H]

COG id: COG1478

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CofE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002847
- InterPro:   IPR008225 [H]

Pfam domain/function: PF01996 F420_ligase [H]

EC number: NA

Molecular weight: Translated: 26394; Mature: 26263

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVRRALEIFALEGVPLVEPGDDLAGLILASLADCGRHLQDHDIVVIAQKVVSKAEGRGV
CCHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHCCCCE
DLAAVTPSPRAVEIAAQAHKDPRIVELILAESSEVLRVRPGVIIVEHRLGLVLANAGIDR
EEEEECCCCCEEEEEECCCCCCCEEEEEECCCCCEEEECCCEEEEEECCCEEEEECCCCC
SNVEGETALLLPLDPDGSAQQIREQLIAGTGATVGVMIIDSIGRAWRNGTIGTTIGASGI
CCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHH
ATMRDLRGQPDLFGRKLETTEVGAADEVAAAASLLMGQAAEATPVVVVRGVDLAGIGTAR
HHHHHCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCEECCCCHH
DLLRPKHMDLFR
HHCCCCHHCCCC
>Mature Secondary Structure 
SVRRALEIFALEGVPLVEPGDDLAGLILASLADCGRHLQDHDIVVIAQKVVSKAEGRGV
CHHHHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHCCCCE
DLAAVTPSPRAVEIAAQAHKDPRIVELILAESSEVLRVRPGVIIVEHRLGLVLANAGIDR
EEEEECCCCCEEEEEECCCCCCCEEEEEECCCCCEEEECCCEEEEEECCCEEEEECCCCC
SNVEGETALLLPLDPDGSAQQIREQLIAGTGATVGVMIIDSIGRAWRNGTIGTTIGASGI
CCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHH
ATMRDLRGQPDLFGRKLETTEVGAADEVAAAASLLMGQAAEATPVVVVRGVDLAGIGTAR
HHHHHCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCEECCCCHH
DLLRPKHMDLFR
HHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]