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The map label for this gene is bioD

Identifier: 86751445

GI number: 86751445

Start: 4928127

End: 4928768

Strand: Reverse

Name: bioD

Synonym: RPB_4343

Alternate gene names: 86751445

Gene position: 4928768-4928127 (Counterclockwise)

Preceding gene: 86751446

Following gene: 86751444

Centisome position: 92.44

GC content: 67.91

Gene sequence:

>642_bases
ATGACCAAGCCCATCGTCGTCACCGGCACCGACACCGGCATCGGCAAGACCGTGTTCGCCGCTGCGCTCGCCGGCGCGCT
CGATGCTTTCTACTGGAAGCCGGTGCAGGCCGGCATCGAGGACGAGACCGACCGGCAGGCGGTGCTGCGGCTGTCCGGCC
TGCCTGACGCGCGCCTGTTGCCGGAGGCGTATCGGCTGAACACGCCGGCCTCGCCACATCTGGCCGCGGAGATCGACGGC
GTGACGATCGATGCCGAAGCGCTCGCGCTGCCGGAGATCGACGGACCGCTGGTGGTCGAGGGCGCCGGTGGGCTGCTGGT
GCCGCTGACGCGCGCGATCACTTACATCGATATCTTCGCACGATGGCGCGCGCCGGTGGTGCTGTGCGCGCGCACCACGC
TCGGCACCATCAATCACACGCTGTTGTCGATCGAGGCGTTGCGTGCGCGCGCGATTCCGCTGCTCGGCGTTGCCTTCATC
GGCGACGAGAATGTCGAATCCGAGCGCGTCATCGTCCAGATCGGCCGCGTGCCGCGATTGGGCCGCCTTCCGCGCCTGGT
CGAGGTGACCGCCGATGCGTTGCGCGCCGCGTTCGCGCAGAACTTCAATACCGACGACTTCCTGAAAGACCCCGCTTCAT
GA

Upstream 100 bases:

>100_bases
AAGGCACCGCGCGGCTGCGCATCGCGCTCACCGCCAATGTCGGCGAGGCGACGATCGCGGATCTGTTTGCGGCGATCGCC
GAAGACATGCGGAAGGCGGC

Downstream 100 bases:

>100_bases
CATCGACTTCGCCGGTCTGGCATCCCTTCACTCAGCACGCGGTGCAGCCCGAGGCGACGTTGATCGCCAAAGGCGAGGGC
GCCTGGCTCGAGACCGCCGA

Product: dithiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MTKPIVVTGTDTGIGKTVFAAALAGALDAFYWKPVQAGIEDETDRQAVLRLSGLPDARLLPEAYRLNTPASPHLAAEIDG
VTIDAEALALPEIDGPLVVEGAGGLLVPLTRAITYIDIFARWRAPVVLCARTTLGTINHTLLSIEALRARAIPLLGVAFI
GDENVESERVIVQIGRVPRLGRLPRLVEVTADALRAAFAQNFNTDDFLKDPAS

Sequences:

>Translated_213_residues
MTKPIVVTGTDTGIGKTVFAAALAGALDAFYWKPVQAGIEDETDRQAVLRLSGLPDARLLPEAYRLNTPASPHLAAEIDG
VTIDAEALALPEIDGPLVVEGAGGLLVPLTRAITYIDIFARWRAPVVLCARTTLGTINHTLLSIEALRARAIPLLGVAFI
GDENVESERVIVQIGRVPRLGRLPRLVEVTADALRAAFAQNFNTDDFLKDPAS
>Mature_212_residues
TKPIVVTGTDTGIGKTVFAAALAGALDAFYWKPVQAGIEDETDRQAVLRLSGLPDARLLPEAYRLNTPASPHLAAEIDGV
TIDAEALALPEIDGPLVVEGAGGLLVPLTRAITYIDIFARWRAPVVLCARTTLGTINHTLLSIEALRARAIPLLGVAFIG
DENVESERVIVQIGRVPRLGRLPRLVEVTADALRAAFAQNFNTDDFLKDPAS

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family

Homologues:

Organism=Escherichia coli, GI1786995, Length=220, Percent_Identity=30.9090909090909, Blast_Score=82, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324385, Length=191, Percent_Identity=36.1256544502618, Blast_Score=89, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOD_RHOP2 (Q2IRY0)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_487941.1
- ProteinModelPortal:   Q2IRY0
- SMR:   Q2IRY0
- STRING:   Q2IRY0
- GeneID:   3912156
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_4343
- eggNOG:   COG0132
- HOGENOM:   HBG650065
- OMA:   WKPIQSG
- ProtClustDB:   PRK00090
- BioCyc:   RPAL316058:RPB_4343-MONOMER
- HAMAP:   MF_00336
- InterPro:   IPR004472
- PIRSF:   PIRSF006755
- TIGRFAMs:   TIGR00347

Pfam domain/function: NA

EC number: =6.3.3.3

Molecular weight: Translated: 22740; Mature: 22609

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKPIVVTGTDTGIGKTVFAAALAGALDAFYWKPVQAGIEDETDRQAVLRLSGLPDARLL
CCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCHHHC
PEAYRLNTPASPHLAAEIDGVTIDAEALALPEIDGPLVVEGAGGLLVPLTRAITYIDIFA
CHHHCCCCCCCCCEEEEECCEEECHHHEECCCCCCCEEEECCCCCHHHHHHHHHHHHHHH
RWRAPVVLCARTTLGTINHTLLSIEALRARAIPLLGVAFIGDENVESERVIVQIGRVPRL
HHCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEEECCCCCC
GRLPRLVEVTADALRAAFAQNFNTDDFLKDPAS
CCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCC
>Mature Secondary Structure 
TKPIVVTGTDTGIGKTVFAAALAGALDAFYWKPVQAGIEDETDRQAVLRLSGLPDARLL
CCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCHHHC
PEAYRLNTPASPHLAAEIDGVTIDAEALALPEIDGPLVVEGAGGLLVPLTRAITYIDIFA
CHHHCCCCCCCCCEEEEECCEEECHHHEECCCCCCCEEEECCCCCHHHHHHHHHHHHHHH
RWRAPVVLCARTTLGTINHTLLSIEALRARAIPLLGVAFIGDENVESERVIVQIGRVPRL
HHCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEEECCCCCC
GRLPRLVEVTADALRAAFAQNFNTDDFLKDPAS
CCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA