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The map label for this gene is paaG [H]

Identifier: 86750715

GI number: 86750715

Start: 4137171

End: 4137971

Strand: Reverse

Name: paaG [H]

Synonym: RPB_3605

Alternate gene names: 86750715

Gene position: 4137971-4137171 (Counterclockwise)

Preceding gene: 86750718

Following gene: 86750714

Centisome position: 77.61

GC content: 64.79

Gene sequence:

>801_bases
ATGCAGTTCAAACACGTCACGCTCGATTTCGACGGCCCGGTCGCGGTCCTCAAGCTCGATCATCCGGAAGTGATGAATGC
GGTCTCGATCGACATGCTGGGCGGCCTCGGCGAGGCGCTCGACGCGATCGAGGACAAGCGCGCCGAAGTGCGCTGCCTGG
TCATCACCGGCGCGGGCCGCGCGTTCTGCACCGGCGCCAATCTGCAGGGCCGCAATTCCGGCAACAACATGAGCCAGAGC
GGCAAGGGCGCCGGCGCCGCGCTCGAGACCGCCTTTCATCCGTTCCTGCGCCGCTTGCGCAAGCTGCATTGCCCGATCGT
CACTTCGGTCAACGGGCCTGCCGCGGGCGCCGGCATGAGCTTCGCGCTGATGGGCGACATGATCCTGTGCGCGCGTTCGT
CCTACTTCCTGCAGGCGTTCCGCCGCATCGGCCTAGTGCCGGATTGCGGCTCGACGTGGTTGCTGCCGCGCCTGGTCGGC
AAGGCGCGCTCGCTCGAATTGTCGCTGCTCGGCGAGAAGCTGCCGGCCGAGAAGGCGCTGGAATGGGGCCTCGTCAATCG
CGTCTACGACGACGCCGAGCTGTGGACCGAAACGATGAAGCTTGCGCAGGAACTCGCCAACGGCCCGACCATAGCGCTGT
CGCTGATCCGCAAGCTGTATTGGGACTCGCCGGAAAATTCGTTCGAGGAGCAGCTCAACCTCGAATTCGAATCCCAGCGC
ATCGCAGGCTCGACCGACGACTTCAAGGAAGGCGTCGGCGCGTTTCTCGAAAAGCGCCCCGCGAAGTTCCAGGGCAAATA
A

Upstream 100 bases:

>100_bases
CGGCGGACCATGGACAAGCGCGCTGCAAAAATCAAATATGGCTCCGGGTTCGCGAGCCTTCCACGCCGCGGAATAACGCG
GAGCAAGCGCGAGGAAACAG

Downstream 100 bases:

>100_bases
GCGCGCGATGGCCGCAACGGCTTTTGTCGAGGCGCTGGCGCGCAGCGTGCGATCGTGGTGCGCCGGCGCGACCGGCGTCC
GCGACGTCGCGCAACTCTCC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MQFKHVTLDFDGPVAVLKLDHPEVMNAVSIDMLGGLGEALDAIEDKRAEVRCLVITGAGRAFCTGANLQGRNSGNNMSQS
GKGAGAALETAFHPFLRRLRKLHCPIVTSVNGPAAGAGMSFALMGDMILCARSSYFLQAFRRIGLVPDCGSTWLLPRLVG
KARSLELSLLGEKLPAEKALEWGLVNRVYDDAELWTETMKLAQELANGPTIALSLIRKLYWDSPENSFEEQLNLEFESQR
IAGSTDDFKEGVGAFLEKRPAKFQGK

Sequences:

>Translated_266_residues
MQFKHVTLDFDGPVAVLKLDHPEVMNAVSIDMLGGLGEALDAIEDKRAEVRCLVITGAGRAFCTGANLQGRNSGNNMSQS
GKGAGAALETAFHPFLRRLRKLHCPIVTSVNGPAAGAGMSFALMGDMILCARSSYFLQAFRRIGLVPDCGSTWLLPRLVG
KARSLELSLLGEKLPAEKALEWGLVNRVYDDAELWTETMKLAQELANGPTIALSLIRKLYWDSPENSFEEQLNLEFESQR
IAGSTDDFKEGVGAFLEKRPAKFQGK
>Mature_266_residues
MQFKHVTLDFDGPVAVLKLDHPEVMNAVSIDMLGGLGEALDAIEDKRAEVRCLVITGAGRAFCTGANLQGRNSGNNMSQS
GKGAGAALETAFHPFLRRLRKLHCPIVTSVNGPAAGAGMSFALMGDMILCARSSYFLQAFRRIGLVPDCGSTWLLPRLVG
KARSLELSLLGEKLPAEKALEWGLVNRVYDDAELWTETMKLAQELANGPTIALSLIRKLYWDSPENSFEEQLNLEFESQR
IAGSTDDFKEGVGAFLEKRPAKFQGK

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=254, Percent_Identity=29.5275590551181, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI31542718, Length=243, Percent_Identity=25.5144032921811, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI116235487, Length=192, Percent_Identity=27.0833333333333, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI4757968, Length=243, Percent_Identity=25.5144032921811, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI49355787, Length=243, Percent_Identity=25.5144032921811, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI4757966, Length=199, Percent_Identity=27.1356783919598, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI51538581, Length=199, Percent_Identity=27.1356783919598, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI25453481, Length=245, Percent_Identity=26.1224489795918, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI51538573, Length=245, Percent_Identity=26.1224489795918, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI70995211, Length=256, Percent_Identity=25.390625, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI45643119, Length=222, Percent_Identity=23.4234234234234, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI260275230, Length=222, Percent_Identity=23.4234234234234, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI260274832, Length=222, Percent_Identity=23.4234234234234, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI221136756, Length=193, Percent_Identity=27.979274611399, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI221136753, Length=193, Percent_Identity=27.979274611399, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI221307494, Length=193, Percent_Identity=27.979274611399, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1787660, Length=253, Percent_Identity=44.2687747035573, Blast_Score=197, Evalue=5e-52,
Organism=Escherichia coli, GI221142681, Length=262, Percent_Identity=30.9160305343511, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1787659, Length=253, Percent_Identity=30.4347826086957, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI87082183, Length=273, Percent_Identity=24.5421245421245, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1788597, Length=261, Percent_Identity=26.0536398467433, Blast_Score=78, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17554946, Length=253, Percent_Identity=28.4584980237154, Blast_Score=112, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI25145438, Length=255, Percent_Identity=30.9803921568627, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17560910, Length=252, Percent_Identity=26.984126984127, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17540306, Length=243, Percent_Identity=26.7489711934156, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17535521, Length=250, Percent_Identity=24.4, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17534483, Length=256, Percent_Identity=23.828125, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17536985, Length=228, Percent_Identity=24.1228070175439, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17558304, Length=216, Percent_Identity=27.3148148148148, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI20129971, Length=250, Percent_Identity=31.2, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24653477, Length=250, Percent_Identity=31.2, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24654903, Length=220, Percent_Identity=26.3636363636364, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI19922422, Length=250, Percent_Identity=24.8, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19920382, Length=250, Percent_Identity=26, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24653139, Length=266, Percent_Identity=27.4436090225564, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21357171, Length=257, Percent_Identity=25.6809338521401, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR011968 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 29002; Mature: 29002

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFKHVTLDFDGPVAVLKLDHPEVMNAVSIDMLGGLGEALDAIEDKRAEVRCLVITGAGR
CCCEEEEEECCCCEEEEEECCHHHHHHHHHHHHHCHHHHHHHHHCCCCCEEEEEEECCCC
AFCTGANLQGRNSGNNMSQSGKGAGAALETAFHPFLRRLRKLHCPIVTSVNGPAAGAGMS
EEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCH
FALMGDMILCARSSYFLQAFRRIGLVPDCGSTWLLPRLVGKARSLELSLLGEKLPAEKAL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCHHCCHHHHHCCCCCHHHHH
EWGLVNRVYDDAELWTETMKLAQELANGPTIALSLIRKLYWDSPENSFEEQLNLEFESQR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCCCCHH
IAGSTDDFKEGVGAFLEKRPAKFQGK
CCCCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MQFKHVTLDFDGPVAVLKLDHPEVMNAVSIDMLGGLGEALDAIEDKRAEVRCLVITGAGR
CCCEEEEEECCCCEEEEEECCHHHHHHHHHHHHHCHHHHHHHHHCCCCCEEEEEEECCCC
AFCTGANLQGRNSGNNMSQSGKGAGAALETAFHPFLRRLRKLHCPIVTSVNGPAAGAGMS
EEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCH
FALMGDMILCARSSYFLQAFRRIGLVPDCGSTWLLPRLVGKARSLELSLLGEKLPAEKAL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCHHCCHHHHHCCCCCHHHHH
EWGLVNRVYDDAELWTETMKLAQELANGPTIALSLIRKLYWDSPENSFEEQLNLEFESQR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCCCCHH
IAGSTDDFKEGVGAFLEKRPAKFQGK
CCCCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]