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The map label for this gene is potF [H]

Identifier: 86750471

GI number: 86750471

Start: 3842918

End: 3844033

Strand: Reverse

Name: potF [H]

Synonym: RPB_3360

Alternate gene names: 86750471

Gene position: 3844033-3842918 (Counterclockwise)

Preceding gene: 86750472

Following gene: 86750462

Centisome position: 72.1

GC content: 62.99

Gene sequence:

>1116_bases
ATGATGCGGAACGATCTCCTTCGACGGCTGCGATTCGTCGGCGCGACGCTGGGGCTTTCGGCGAGCCTCGCCGTGCCGGC
GCTGGCGCAGGATCGCGTGGTCAATTTCTACAACTGGTCGAACTACGTCGCGCCCGGCGTACTCGAGGAGTTCACCCGCG
AAACCGGGATCAAGGTGATCTACGACACCTTCGACGGCAACGAGACGCTGGAGACCAAGCTGCTCGCCGGCAAATCCGGC
TACGACGTCGTCGTGCCGACCGCGTATTTCCTGCAGCGCCAGATCGGCGCGAAGGTGTTCCAGAAGCTCGACCCGACGAA
GCTGCCCAACCTGAAGAACGCCTGGGACGTGGTGACGAAGAAGCTGGCGCTGTACGATCCCGGCAATCACTATGCGGCCA
ACTACATGTGGGGGACCACCGGCATCGGCTACAATGTCGGCGCAGTGAAGCGCATTCTCGGCGAGGGTGCGGTGATCGAC
AGCTGGGACATCGTGTTCAAGCCGGAGAATCTGGCGAAGTTCAAGGAGTGCGGCGTCCAGATGCTGGATTCCGCCGACGA
CATCCTGCCGGCGGCGCTGACCCGTCTCGGCCTCGATCCGAACTCGACCAAGCAGCCGGATCTGGAGAAGGCCGCCGATG
CCGTCGCCAAGGTGCGGCCGTCGGTGCGGAAGTTTCACTCGTCGGAATATCTCAACGCGCTCGCCACCGGCGAAATCTGC
CTCGTCGTCGGCTGGTCCGGCGATATCAAGCAGGCGCAGGCGCGGGCGACCGAAGCCAACAACGGCGTCGAAATCGGCTA
CGCGATCCCGAAAGAAGGAGCGCAGATGTTCTTCGACAATCTGGCTATCCCGGCGGACGCCAAGAACGTCGCCGAGGCGC
ACGAATTGATCAACTTCCTGTTTCGCCCCGAGATCGCCGCCCGCAATTCCGACTTCCTGTCCTACGCCAACGGCAACAAA
GCCAGCCAGGAATTCGTCAACCCGCGTATCTTGAACGACAAGACGATCTATCCGGACGAAGCGATGCAGGCGCGCCTGTT
CGTCATCACCGCGCGCGACGCGGCGACGCAGCGCGTGATCAACCGGCTGTGGACGCGGGTGAAGACCGGGCGGTGA

Upstream 100 bases:

>100_bases
GTCACTTCGGTGGTGCGGTTCTGTTTCGCCAAACAGGATCAGACGCTCGACACCGCGCTCGAGCGGCTGTCGGATGCGGT
GCACAGGCGTTAGGACTTCG

Downstream 100 bases:

>100_bases
AACCGTCCCTGGTCAGGCAGTCCCCGCCATGTCCCGCGCCGACGCTTCGCGAACGACGGGCTTTCCGAGGTCGCGCAGAT
CAATCGTTCCCGGGAGCGCG

Product: extracellular solute-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MMRNDLLRRLRFVGATLGLSASLAVPALAQDRVVNFYNWSNYVAPGVLEEFTRETGIKVIYDTFDGNETLETKLLAGKSG
YDVVVPTAYFLQRQIGAKVFQKLDPTKLPNLKNAWDVVTKKLALYDPGNHYAANYMWGTTGIGYNVGAVKRILGEGAVID
SWDIVFKPENLAKFKECGVQMLDSADDILPAALTRLGLDPNSTKQPDLEKAADAVAKVRPSVRKFHSSEYLNALATGEIC
LVVGWSGDIKQAQARATEANNGVEIGYAIPKEGAQMFFDNLAIPADAKNVAEAHELINFLFRPEIAARNSDFLSYANGNK
ASQEFVNPRILNDKTIYPDEAMQARLFVITARDAATQRVINRLWTRVKTGR

Sequences:

>Translated_371_residues
MMRNDLLRRLRFVGATLGLSASLAVPALAQDRVVNFYNWSNYVAPGVLEEFTRETGIKVIYDTFDGNETLETKLLAGKSG
YDVVVPTAYFLQRQIGAKVFQKLDPTKLPNLKNAWDVVTKKLALYDPGNHYAANYMWGTTGIGYNVGAVKRILGEGAVID
SWDIVFKPENLAKFKECGVQMLDSADDILPAALTRLGLDPNSTKQPDLEKAADAVAKVRPSVRKFHSSEYLNALATGEIC
LVVGWSGDIKQAQARATEANNGVEIGYAIPKEGAQMFFDNLAIPADAKNVAEAHELINFLFRPEIAARNSDFLSYANGNK
ASQEFVNPRILNDKTIYPDEAMQARLFVITARDAATQRVINRLWTRVKTGR
>Mature_371_residues
MMRNDLLRRLRFVGATLGLSASLAVPALAQDRVVNFYNWSNYVAPGVLEEFTRETGIKVIYDTFDGNETLETKLLAGKSG
YDVVVPTAYFLQRQIGAKVFQKLDPTKLPNLKNAWDVVTKKLALYDPGNHYAANYMWGTTGIGYNVGAVKRILGEGAVID
SWDIVFKPENLAKFKECGVQMLDSADDILPAALTRLGLDPNSTKQPDLEKAADAVAKVRPSVRKFHSSEYLNALATGEIC
LVVGWSGDIKQAQARATEANNGVEIGYAIPKEGAQMFFDNLAIPADAKNVAEAHELINFLFRPEIAARNSDFLSYANGNK
ASQEFVNPRILNDKTIYPDEAMQARLFVITARDAATQRVINRLWTRVKTGR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=367, Percent_Identity=48.7738419618529, Blast_Score=374, Evalue=1e-105,
Organism=Escherichia coli, GI1787367, Length=306, Percent_Identity=38.562091503268, Blast_Score=203, Evalue=1e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 41026; Mature: 41026

Theoretical pI: Translated: 8.55; Mature: 8.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRNDLLRRLRFVGATLGLSASLAVPALAQDRVVNFYNWSNYVAPGVLEEFTRETGIKVI
CCHHHHHHHHHHHHHHHCCCCHHCCCHHHHCCEEEEECCCCCCCCHHHHHHHHHCCCEEE
YDTFDGNETLETKLLAGKSGYDVVVPTAYFLQRQIGAKVFQKLDPTKLPNLKNAWDVVTK
EEECCCCCHHHHHEEECCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
KLALYDPGNHYAANYMWGTTGIGYNVGAVKRILGEGAVIDSWDIVFKPENLAKFKECGVQ
HHHEECCCCCEEEEEEEECCCCCCCHHHHHHHHCCCCEEECCEEEECCHHHHHHHHHHHH
MLDSADDILPAALTRLGLDPNSTKQPDLEKAADAVAKVRPSVRKFHSSEYLNALATGEIC
HHCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEE
LVVGWSGDIKQAQARATEANNGVEIGYAIPKEGAQMFFDNLAIPADAKNVAEAHELINFL
EEEECCCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
FRPEIAARNSDFLSYANGNKASQEFVNPRILNDKTIYPDEAMQARLFVITARDAATQRVI
HCCCHHCCCCCCEEECCCCHHHHHHCCCEECCCCCCCCCHHHCEEEEEEECCHHHHHHHH
NRLWTRVKTGR
HHHHHHHCCCC
>Mature Secondary Structure
MMRNDLLRRLRFVGATLGLSASLAVPALAQDRVVNFYNWSNYVAPGVLEEFTRETGIKVI
CCHHHHHHHHHHHHHHHCCCCHHCCCHHHHCCEEEEECCCCCCCCHHHHHHHHHCCCEEE
YDTFDGNETLETKLLAGKSGYDVVVPTAYFLQRQIGAKVFQKLDPTKLPNLKNAWDVVTK
EEECCCCCHHHHHEEECCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
KLALYDPGNHYAANYMWGTTGIGYNVGAVKRILGEGAVIDSWDIVFKPENLAKFKECGVQ
HHHEECCCCCEEEEEEEECCCCCCCHHHHHHHHCCCCEEECCEEEECCHHHHHHHHHHHH
MLDSADDILPAALTRLGLDPNSTKQPDLEKAADAVAKVRPSVRKFHSSEYLNALATGEIC
HHCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEE
LVVGWSGDIKQAQARATEANNGVEIGYAIPKEGAQMFFDNLAIPADAKNVAEAHELINFL
EEEECCCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
FRPEIAARNSDFLSYANGNKASQEFVNPRILNDKTIYPDEAMQARLFVITARDAATQRVI
HCCCHHCCCCCCEEECCCCHHHHHHCCCEECCCCCCCCCHHHCEEEEEEECCHHHHHHHH
NRLWTRVKTGR
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]