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The map label for this gene is miaA

Identifier: 86750457

GI number: 86750457

Start: 3828206

End: 3829138

Strand: Reverse

Name: miaA

Synonym: RPB_3346

Alternate gene names: 86750457

Gene position: 3829138-3828206 (Counterclockwise)

Preceding gene: 86750462

Following gene: 86750456

Centisome position: 71.82

GC content: 70.74

Gene sequence:

>933_bases
GTGCTTATCGCAGGGCCGACCGCCAGCGGCAAGTCGGCGCTCGCGCTGCGGCTTGCGCAGATGCACGGCGGCGTCGTCAT
CAACACCGATTCGATGCAGATGTACCGGGACCTGCGGATCATCACCGCCCGGCCGACGCCGGAAGAGGAGGCGCTGGTCC
CGCATCGGCTCTACGGCACGGTGGACGCCGCGGTGAATTTCTCCGCTGGCGCCTTCGTCGAGGCCGCTGCCGGGGCGCTG
GCCGAGGCGCGCGCTTCGGGCCTGCTGCCGATCTTCATCGGCGGCACCGGGCTGTATTTCAAGGCGCTGACCCGCGGACT
GTCTTTGGTGCCGCCGGTGCCGGCCGAAATCCGCGACGCGGTGCGGCTGCGGCTCGACCGCGATGGCGTGGCGGCGTTGC
ACGCCGAACTGGCCCGGCACGATCCGGCCGCAGCCGAACGGCTGGCCCCGGCCGACCGATCACGGATCGCGCGGGCGCTG
GAAGTGGTGCTGGCGACCGGTCGGCCATTGGCCGACTGGCACGGCGAGGCCTCGCCGCCGCTATTGCCGCCGGACGGCGT
CACGGCGGTGTTTCTCGCGCCCGAGCGCGAGGCGCTGTACGCCCGGATCGACGCCCGATTCGCCGCAATGCTGCGGGCCG
GCGCGCTGGACGAGGTCGCGGCCTTGGCCGAGCGCCGGCTCGACCCGCTGCTGCCGGCGATGAAGGCGCATGGCGTGCCG
GCGCTGATCCGACATCTGCGCGGCGAGATTTCGCTGGACGAGGCGGCCGTCATCGGCGCCGCCGACACGCGGCACTACGC
CAAGCGGCAATTCACCTGGTTCCGGCACCAGTTACCAGAGTTTCGTTGGGTGACGCCTGAGGAGGCGGGGAGGGTGGTGG
ATCAAGAATTTCAGGGCAATCCAAGCTGCACCACCAGCACAGCGTCATCCTGA

Upstream 100 bases:

>100_bases
CACGGTGGAATCCAGCGCGGGACTGTTTGGATTGCAGATGAGCGTGGCGACGAGCGACATGGGAAAAACGGTTCCGGAGC
AGGGGCAAAAGCCGGCGGCC

Downstream 100 bases:

>100_bases
GGTGCGAGCGCAGCGAGCCTCGAAGGATGGAACGACGAAGTCCGTGCCGCCCATCCTGAGGTGCTGCAAAGGTCCGTGAG
ACGGACCTTTGCAGCACCTC

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MLIAGPTASGKSALALRLAQMHGGVVINTDSMQMYRDLRIITARPTPEEEALVPHRLYGTVDAAVNFSAGAFVEAAAGAL
AEARASGLLPIFIGGTGLYFKALTRGLSLVPPVPAEIRDAVRLRLDRDGVAALHAELARHDPAAAERLAPADRSRIARAL
EVVLATGRPLADWHGEASPPLLPPDGVTAVFLAPEREALYARIDARFAAMLRAGALDEVAALAERRLDPLLPAMKAHGVP
ALIRHLRGEISLDEAAVIGAADTRHYAKRQFTWFRHQLPEFRWVTPEEAGRVVDQEFQGNPSCTTSTASS

Sequences:

>Translated_310_residues
MLIAGPTASGKSALALRLAQMHGGVVINTDSMQMYRDLRIITARPTPEEEALVPHRLYGTVDAAVNFSAGAFVEAAAGAL
AEARASGLLPIFIGGTGLYFKALTRGLSLVPPVPAEIRDAVRLRLDRDGVAALHAELARHDPAAAERLAPADRSRIARAL
EVVLATGRPLADWHGEASPPLLPPDGVTAVFLAPEREALYARIDARFAAMLRAGALDEVAALAERRLDPLLPAMKAHGVP
ALIRHLRGEISLDEAAVIGAADTRHYAKRQFTWFRHQLPEFRWVTPEEAGRVVDQEFQGNPSCTTSTASS
>Mature_310_residues
MLIAGPTASGKSALALRLAQMHGGVVINTDSMQMYRDLRIITARPTPEEEALVPHRLYGTVDAAVNFSAGAFVEAAAGAL
AEARASGLLPIFIGGTGLYFKALTRGLSLVPPVPAEIRDAVRLRLDRDGVAALHAELARHDPAAAERLAPADRSRIARAL
EVVLATGRPLADWHGEASPPLLPPDGVTAVFLAPEREALYARIDARFAAMLRAGALDEVAALAERRLDPLLPAMKAHGVP
ALIRHLRGEISLDEAAVIGAADTRHYAKRQFTWFRHQLPEFRWVTPEEAGRVVDQEFQGNPSCTTSTASS

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=309, Percent_Identity=27.5080906148867, Blast_Score=106, Evalue=3e-23,
Organism=Escherichia coli, GI1790613, Length=300, Percent_Identity=37.6666666666667, Blast_Score=171, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI25144712, Length=237, Percent_Identity=25.7383966244726, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324848, Length=293, Percent_Identity=29.0102389078498, Blast_Score=129, Evalue=9e-31,
Organism=Drosophila melanogaster, GI24649830, Length=236, Percent_Identity=32.6271186440678, Blast_Score=103, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_RHOP2 (Q2IUR8)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_486953.1
- ProteinModelPortal:   Q2IUR8
- STRING:   Q2IUR8
- GeneID:   3911148
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_3346
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- BioCyc:   RPAL316058:RPB_3346-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 33228; Mature: 33228

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIAGPTASGKSALALRLAQMHGGVVINTDSMQMYRDLRIITARPTPEEEALVPHRLYGT
CEECCCCCCCCHHHHHHHHHHCCCEEEECCHHHHHHCEEEEECCCCCCCCCCCCHHHHHH
VDAAVNFSAGAFVEAAAGALAEARASGLLPIFIGGTGLYFKALTRGLSLVPPVPAEIRDA
HHHHEECCCCHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHCCCCCCCCCCHHHHHH
VRLRLDRDGVAALHAELARHDPAAAERLAPADRSRIARALEVVLATGRPLADWHGEASPP
HHHHCCCCCHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHCCCCCCCC
LLPPDGVTAVFLAPEREALYARIDARFAAMLRAGALDEVAALAERRLDPLLPAMKAHGVP
CCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHH
ALIRHLRGEISLDEAAVIGAADTRHYAKRQFTWFRHQLPEFRWVTPEEAGRVVDQEFQGN
HHHHHHCCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCEECCHHHHCCHHHHHHCCC
PSCTTSTASS
CCCCCCCCCC
>Mature Secondary Structure
MLIAGPTASGKSALALRLAQMHGGVVINTDSMQMYRDLRIITARPTPEEEALVPHRLYGT
CEECCCCCCCCHHHHHHHHHHCCCEEEECCHHHHHHCEEEEECCCCCCCCCCCCHHHHHH
VDAAVNFSAGAFVEAAAGALAEARASGLLPIFIGGTGLYFKALTRGLSLVPPVPAEIRDA
HHHHEECCCCHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHCCCCCCCCCCHHHHHH
VRLRLDRDGVAALHAELARHDPAAAERLAPADRSRIARALEVVLATGRPLADWHGEASPP
HHHHCCCCCHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHCCCCCCCC
LLPPDGVTAVFLAPEREALYARIDARFAAMLRAGALDEVAALAERRLDPLLPAMKAHGVP
CCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHH
ALIRHLRGEISLDEAAVIGAADTRHYAKRQFTWFRHQLPEFRWVTPEEAGRVVDQEFQGN
HHHHHHCCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCEECCHHHHCCHHHHHHCCC
PSCTTSTASS
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA