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The map label for this gene is leuA

Identifier: 86750441

GI number: 86750441

Start: 3809433

End: 3811007

Strand: Reverse

Name: leuA

Synonym: RPB_3330

Alternate gene names: 86750441

Gene position: 3811007-3809433 (Counterclockwise)

Preceding gene: 86750443

Following gene: 86750440

Centisome position: 71.48

GC content: 64.95

Gene sequence:

>1575_bases
ATGACCACGACTCCGAAGTCCGAGCAGGACCGCGTCATCATTTTCGACACCACCTTGCGCGACGGCGAGCAGTGCCCCGG
CGCCACCATGACGTTCGAGGAGAAGCTGAACGTCGCCAGGATGCTCGACGACATGGGCGTCGACGTCATCGAGGCCGGTT
ATCCGTTTGCTTCCGACGGTGACTTCGAGGCGGTGCACGAGATCGCCAAGCGCTCGAAGAATTCGGTGATCTGCGGATTG
TCGCGCGCGTCGCACAAGGACATCGACCGCTGCGCCGAGGCGATCAAGCCGGCCGAGCGCGGCCGCATCCACACCTTCCT
GTCCACCTCGCCGGTGCACATGAAGTACAAGCTGCAGATGGAAGCAGCCCAGGTCTACGAGATGGTGATCTCGTCGGTGA
CCCGCGCCCGCAACCACACCGACGACGTCGAGTGGTCGGCCGAGGACGCCACCCGCACCGAGTTCGATTTCCTGTGCCGC
TGCATTGAGGCCGCGATCAAGGCCGGCGCCACCACCATCAACCTGCCGGACACGGTCGGCTACGCGGTGCCGGAGGAATA
TCGCGAGATGTTCCGCAAGGTGCGCGAGACGGTGCCGAATTCCGACAAGGCGCGGTTCTCGGTGCACTGCCACAACGACC
TCGGCATGGCGGTGGCCAATTCGATGGCCGGCGTCGCCGGCGGCGCGCGGCAGATCGAGTGCACCATCAACGGCATCGGC
GAGCGCGCCGGCAACGCCGCGCTGGAAGAACTGGTGATGGCGATGCGGGTCCGGCAGGACAAATTGCCGTACTGGAACAA
CATCGAAACCACGATGCTGACCCACGCCTCCAAGACGGTGTCGGCCGCGACTTCGTTCCCGGTGCAGTACAACAAGGCGA
TCGTCGGCCGCAACGCGTTCGCGCACGAGAGCGGCATCCATCAGGACGGCATGATCAAGAATGCCCAGACCTACGAGATC
ATGACGCCGGAGACGGTCGGCGTGAAGGGCACCTCGCTGGTGATGGGCAAGCATTCCGGCCGCGCCGGACTGATCCACAA
GCTGGAAGAGCTGGGCTACAAGCTGTCGCGCAACCAGATCGAGGACGTGTTCGTGCGGTTCAAGGCTTTGGCCGACCGCA
AGAAGGACGTCTACGACGAGGACATCGAGGCGCTGGTCGACGAGCAGTTGCTGCACGGCCAGGATCAGATCAAGCTGAAC
TCGCTGACGGTGATCGCCGGCACCCACGGCCCGCAGCGCGCCACCATGAAGCTCGACGTCGACGGCCAGATTAGAATCGA
GGAAGCCGAAGGCAACGGCCCGGTCGACGCGGTGTTCAACTGCATCAAGGCGCTGGTGCCGCACGACGCCAAGCTGGAGT
TGTATCAGGTTCACGCCGTTACCGAAGGTACCGATGCGCAGGCCGAAGTCTCGGTCCGGCTGTCGCACGAGGGCCGCTCG
ATGACCGCGCGCGCCGCCGATCCGGATACGCTGGTGGCGTCGGCCAAGGCCTATCTGGGCGCGCTCAACAAGATCGTCGC
CAAGCGCCAGCGCGACGTCCGGGAGGCAGTGCCCGCGGTCGCGGCGGCCGGCTGA

Upstream 100 bases:

>100_bases
CCTGAGGGCCGTCTGGGCGTTTGCGCCTGGGCACTCAGGGGTCCGCACGAACGCCAGCCCTTCATGCGCCCGACGGCGCC
CCGACCAAAGGAATTCTCTC

Downstream 100 bases:

>100_bases
GCCGCCTTCATCACGCGATCACGAAGCCGGGGGCGGTGCATATTGCGCCGCCCTCTTTTGCGTTGCAGCAACTTTTTGCC
GCGCCCCTGCTAAAGGGGCA

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase

Number of amino acids: Translated: 524; Mature: 523

Protein sequence:

>524_residues
MTTTPKSEQDRVIIFDTTLRDGEQCPGATMTFEEKLNVARMLDDMGVDVIEAGYPFASDGDFEAVHEIAKRSKNSVICGL
SRASHKDIDRCAEAIKPAERGRIHTFLSTSPVHMKYKLQMEAAQVYEMVISSVTRARNHTDDVEWSAEDATRTEFDFLCR
CIEAAIKAGATTINLPDTVGYAVPEEYREMFRKVRETVPNSDKARFSVHCHNDLGMAVANSMAGVAGGARQIECTINGIG
ERAGNAALEELVMAMRVRQDKLPYWNNIETTMLTHASKTVSAATSFPVQYNKAIVGRNAFAHESGIHQDGMIKNAQTYEI
MTPETVGVKGTSLVMGKHSGRAGLIHKLEELGYKLSRNQIEDVFVRFKALADRKKDVYDEDIEALVDEQLLHGQDQIKLN
SLTVIAGTHGPQRATMKLDVDGQIRIEEAEGNGPVDAVFNCIKALVPHDAKLELYQVHAVTEGTDAQAEVSVRLSHEGRS
MTARAADPDTLVASAKAYLGALNKIVAKRQRDVREAVPAVAAAG

Sequences:

>Translated_524_residues
MTTTPKSEQDRVIIFDTTLRDGEQCPGATMTFEEKLNVARMLDDMGVDVIEAGYPFASDGDFEAVHEIAKRSKNSVICGL
SRASHKDIDRCAEAIKPAERGRIHTFLSTSPVHMKYKLQMEAAQVYEMVISSVTRARNHTDDVEWSAEDATRTEFDFLCR
CIEAAIKAGATTINLPDTVGYAVPEEYREMFRKVRETVPNSDKARFSVHCHNDLGMAVANSMAGVAGGARQIECTINGIG
ERAGNAALEELVMAMRVRQDKLPYWNNIETTMLTHASKTVSAATSFPVQYNKAIVGRNAFAHESGIHQDGMIKNAQTYEI
MTPETVGVKGTSLVMGKHSGRAGLIHKLEELGYKLSRNQIEDVFVRFKALADRKKDVYDEDIEALVDEQLLHGQDQIKLN
SLTVIAGTHGPQRATMKLDVDGQIRIEEAEGNGPVDAVFNCIKALVPHDAKLELYQVHAVTEGTDAQAEVSVRLSHEGRS
MTARAADPDTLVASAKAYLGALNKIVAKRQRDVREAVPAVAAAG
>Mature_523_residues
TTTPKSEQDRVIIFDTTLRDGEQCPGATMTFEEKLNVARMLDDMGVDVIEAGYPFASDGDFEAVHEIAKRSKNSVICGLS
RASHKDIDRCAEAIKPAERGRIHTFLSTSPVHMKYKLQMEAAQVYEMVISSVTRARNHTDDVEWSAEDATRTEFDFLCRC
IEAAIKAGATTINLPDTVGYAVPEEYREMFRKVRETVPNSDKARFSVHCHNDLGMAVANSMAGVAGGARQIECTINGIGE
RAGNAALEELVMAMRVRQDKLPYWNNIETTMLTHASKTVSAATSFPVQYNKAIVGRNAFAHESGIHQDGMIKNAQTYEIM
TPETVGVKGTSLVMGKHSGRAGLIHKLEELGYKLSRNQIEDVFVRFKALADRKKDVYDEDIEALVDEQLLHGQDQIKLNS
LTVIAGTHGPQRATMKLDVDGQIRIEEAEGNGPVDAVFNCIKALVPHDAKLELYQVHAVTEGTDAQAEVSVRLSHEGRSM
TARAADPDTLVASAKAYLGALNKIVAKRQRDVREAVPAVAAAG

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786261, Length=500, Percent_Identity=46.8, Blast_Score=447, Evalue=1e-127,
Organism=Saccharomyces cerevisiae, GI6320071, Length=371, Percent_Identity=28.8409703504043, Blast_Score=146, Evalue=6e-36,
Organism=Saccharomyces cerevisiae, GI6320019, Length=381, Percent_Identity=28.3464566929134, Blast_Score=146, Evalue=9e-36,
Organism=Saccharomyces cerevisiae, GI6324682, Length=539, Percent_Identity=25.2319109461967, Blast_Score=134, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6324225, Length=538, Percent_Identity=25.8364312267658, Blast_Score=128, Evalue=2e-30,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU1_RHOP2 (Q2IUT4)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_486937.1
- ProteinModelPortal:   Q2IUT4
- STRING:   Q2IUT4
- GeneID:   3911132
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_3330
- eggNOG:   COG0119
- HOGENOM:   HBG701974
- OMA:   DQAIISV
- ProtClustDB:   PRK00915
- BioCyc:   RPAL316058:RPB_3330-MONOMER
- HAMAP:   MF_01025_B
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00917
- TIGRFAMs:   TIGR00973

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 57475; Mature: 57344

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTPKSEQDRVIIFDTTLRDGEQCPGATMTFEEKLNVARMLDDMGVDVIEAGYPFASDG
CCCCCCCCCCEEEEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCEEECCCCCCCCC
DFEAVHEIAKRSKNSVICGLSRASHKDIDRCAEAIKPAERGRIHTFLSTSPVHMKYKLQM
CHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHCCHHCCCEEEEECCCCEEEEEEEEH
EAAQVYEMVISSVTRARNHTDDVEWSAEDATRTEFDFLCRCIEAAIKAGATTINLPDTVG
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCC
YAVPEEYREMFRKVRETVPNSDKARFSVHCHNDLGMAVANSMAGVAGGARQIECTINGIG
CCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCEEEEEEEECCC
ERAGNAALEELVMAMRVRQDKLPYWNNIETTMLTHASKTVSAATSFPVQYNKAIVGRNAF
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCHHHHHHHCCCCEECCEEECCCCH
AHESGIHQDGMIKNAQTYEIMTPETVGVKGTSLVMGKHSGRAGLIHKLEELGYKLSRNQI
HHCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHH
EDVFVRFKALADRKKDVYDEDIEALVDEQLLHGQDQIKLNSLTVIAGTHGPQRATMKLDV
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCEEEEEEC
DGQIRIEEAEGNGPVDAVFNCIKALVPHDAKLELYQVHAVTEGTDAQAEVSVRLSHEGRS
CCEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCEEEEEEEECCCCE
MTARAADPDTLVASAKAYLGALNKIVAKRQRDVREAVPAVAAAG
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTTPKSEQDRVIIFDTTLRDGEQCPGATMTFEEKLNVARMLDDMGVDVIEAGYPFASDG
CCCCCCCCCEEEEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCEEECCCCCCCCC
DFEAVHEIAKRSKNSVICGLSRASHKDIDRCAEAIKPAERGRIHTFLSTSPVHMKYKLQM
CHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHCCHHCCCEEEEECCCCEEEEEEEEH
EAAQVYEMVISSVTRARNHTDDVEWSAEDATRTEFDFLCRCIEAAIKAGATTINLPDTVG
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCC
YAVPEEYREMFRKVRETVPNSDKARFSVHCHNDLGMAVANSMAGVAGGARQIECTINGIG
CCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCEEEEEEEECCC
ERAGNAALEELVMAMRVRQDKLPYWNNIETTMLTHASKTVSAATSFPVQYNKAIVGRNAF
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCHHHHHHHCCCCEECCEEECCCCH
AHESGIHQDGMIKNAQTYEIMTPETVGVKGTSLVMGKHSGRAGLIHKLEELGYKLSRNQI
HHCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHH
EDVFVRFKALADRKKDVYDEDIEALVDEQLLHGQDQIKLNSLTVIAGTHGPQRATMKLDV
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCEEEEEEC
DGQIRIEEAEGNGPVDAVFNCIKALVPHDAKLELYQVHAVTEGTDAQAEVSVRLSHEGRS
CCEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCEEEEEEEECCCCE
MTARAADPDTLVASAKAYLGALNKIVAKRQRDVREAVPAVAAAG
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA